FlyBase Allele Report: Dmel\gbb[UAS.cKa]

General Information

Symbol

Dmel\gbb^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Khalsa

FlyBase ID

FBal0092971

Feature type

allele

Associated gene

Dmel\gbb

Associated Insertion(s)

Carried in Construct

P{UAS-gbb.K}

Also Known As

UAS-gbb

Key Links

Transgenic product class

cDNA

(Khalsa et al., 1998)

wild_type

(Khalsa et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Khalsa et al., 1998)

wild_type

(Khalsa et al., 1998)

Mutagen

in vitro construct

(Haerry et al., 1998, Khalsa et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-gbb.K}

Cytology

Description

A 1.6kb EcoRI fragment of a gbb cDNA (containing the entire open reading frame) is expressed under the control of UASt regulatory sequences.

(Khalsa et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

gbb

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of fibrodysplasia ossificans progressiva

CEA with sax^{K262H.UAS.Tag:FLAG}

(Le et al., 2018)

model of obesity

CEA

(Hong et al., 2016)

model of diabetes mellitus

CEA

(Hong et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis type 1

modeled by Sod1^G85R

(Held et al., 2019)

model of obesity

is ameliorated by trbl^VDRC.cUa

(Hong et al., 2016)

model of diabetes mellitus

is ameliorated by trbl^VDRC.cUa

(Hong et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: Co-expression of sax^{K262H.UAS.Tag:FLAG} and gbb^UAS.cKa are required to induce constitutive BMP signalling equivalent to that induced by expression of Hsap\ACVR1^{R206H.UAS.Tag:FLAG} alone.

(Le et al., 2018)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

1st posterior cell, with Scer\GAL4^ptc-559.1

(Khalsa et al., 1998)

anterior crossvein, with Scer\GAL4^ptc-559.1

(Evans et al., 2009)

bouton, with gbb²/gbb¹

(McCabe et al., 2003)

germline cell, with Scer\GAL4^twi.PG

(Deshpande et al., 2014)

NMJ bouton | increased number, with Scer\GAL4^how-24B

(James et al., 2014)

synapse, with gbb²/gbb¹

(McCabe et al., 2003)

synapse & neuromuscular junction, with gbb²/gbb¹

(McCabe et al., 2003)

triple row of wing sensilla, with Scer\GAL4^ap-md544

(Takaesu et al., 2005)

triple row of wing sensilla, with Scer\GAL4^C-765

(Takaesu et al., 2005)

type I bouton | increased number | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(James and Broihier, 2011)

type I bouton | increased number | third instar larval stage, with Scer\GAL4^Toll-6-D42

(James and Broihier, 2011)

wing, with Scer\GAL4^A9

(Le and Wharton, 2012, James and Broihier, 2011, Vilmos et al., 2005, Yu et al., 2000, Haerry et al., 1998, Khalsa et al., 1998)

wing, with Scer\GAL4^ap-md544

(Takaesu et al., 2005)

wing, with Scer\GAL4^C-765

(Takaesu et al., 2005)

wing, with Scer\GAL4^ptc-559.1

(Khalsa et al., 1998)

wing | heat sensitive, with Scer\GAL4^en.PU, Scer\GAL80^ts.αTub84B

(Fritsch et al., 2012)

wing basal cell 1, with Scer\GAL4^ptc-559.1

(Khalsa et al., 1998)

wing blade posterior compartment, with Scer\GAL4^en-e16E

(Haerry et al., 1998)

wing cell, with Scer\GAL4^A9

(Yu et al., 2000)

wing cell, with Scer\GAL4^ptc-559.1

(Khalsa et al., 1998)

wing disc, with Scer\GAL4^en-e16E

(Haerry et al., 1998)

wing vein, with Scer\GAL4^71B

(Khalsa et al., 1998)

wing vein, with Scer\GAL4^A9

(Bangi and Wharton, 2006, Khalsa et al., 1998)

wing vein, with Scer\GAL4^ptc-559.1

(Kang and Bier, 2010)

wing vein | ectopic, with Scer\GAL4^71B

(Khalsa et al., 1998)

wing vein | ectopic, with Scer\GAL4^A9

(Le and Wharton, 2012, Yu et al., 2000)

wing vein | ectopic, with Scer\GAL4^ptc-559.1

(Kang and Bier, 2010, Khalsa et al., 1998)

wing vein L2, with Scer\GAL4^A9

(Haerry et al., 1998)

wing vein L5, with Scer\GAL4^A9

(Haerry et al., 1998)

Detailed Description

Statement

Reference

The expression of gbb^UAS.cKa under the control of Scer\GAL4^A58 does not lead to any obvious third instar larval C4da neuron morphology defects, as compared to controls.

(Hoyer et al., 2018)

Over expression of gbb^Scer\UAS.cKa, under the control of Scer\GAL4^twi.PG results in a relatively minor but statistically significant reduction in the total number of germ cells.

(Deshpande et al., 2014)

Expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^how-24B results in a neuromuscular junction overgrowth phenotype.

(James et al., 2014)

Expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^en.PU from the onset of pupal development (the temperature sensitive allele Scer\GAL80^ts.αTub84B is used to restrict the stage during which expression occurs) results in wing defects which are more prominent in the posterior half.

(Fritsch et al., 2012)

Expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 results in a variable wing phenotype, marked by ectopic vein material.

(Le and Wharton, 2012)

Larvae expressing gbb^Scer\UAS.cKa under the control of Scer\GAL4^D42 display a 40% increase in the number of boutons at neuromuscular junction (NMJ) 6/7 and a 62% increase in type I boutons at NMJ 4. Scer\GAL4^OK6-mediated expression of gbb^Scer\UAS.cKa has a comparable effect.

Overexpression of gbb^Scer\UAS.cKa using Scer\GAL4^A9 results in a severe wing blistering phenotype in 79% of females and a more severe phenotype, including unfurling and blistering of the wing in 93% of males.

(James and Broihier, 2011)

Expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^ptc-559.1 results in an ectopic wing vein phenotype.

(Kang and Bier, 2010)

Expression of gbb^Scer\UAS.cKa in the wing with Scer\GAL4^ptc-559.1 induces an expanded region of ectopic vein where the anterior crossvein would normally form.

(Evans et al., 2009)

Overexpression of gbb^Scer\UAS.cKa post-synaptically under the control of Scer\GAL4^eve.RN2 increases synaptic current amplitude compared to controls.

(Fradkin et al., 2008)

Expression of gbb^Scer\UAS.cKa in the wing disc at 25^oC, under the control of Scer\GAL4^A9, produces wings divided into four classes of phenotypic severity according to the amount of extra wing vein material present in the wing.

(Bangi and Wharton, 2006)

The proximal 25% of the anterior margin of a gbb^Scer\UAS.cKa/Scer\GAL4^ap-md544 wing has no bristles. More distally, the triple row reappears but is quite irregular. When gbb^Scer\UAS.cKa is driven in all the cells of the wing blade with Scer\GAL4^C-765, the phenotype is similar but less severe.

(Takaesu et al., 2005)

Overexpression of gbb^Scer\UAS.cKa, under the regulation of Scer\GAL4^A9, can result in wing disruption.

(Vilmos et al., 2005)

Postsynaptic expression of gbb^UAS.cKa, under the control of Scer\GAL4^eve.RN2, results in the averaged amplitude of synaptic currents recorded from whole-cell voltage clamp recordings of aCC/RP2 motoneurons being significantly increased compared with controls. In contrast, expression of gbb^UAS.cKa in presynaptic cholinergic interneurons, driven by Scer\GAL4^ChAT.7.4, does not affect the averaged synaptic current amplitude.

(Baines, 2004)

gbb^Scer\UAS.cKa; Scer\GAL4^C587 females do not have increased numbers of germ-line stem cells in their germaria.

(Song et al., 2004)

Expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 produces slightly broadened wings consisting of solid veins.

(Yu et al., 2000)

Expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^dpp.blk1 does not produce a mutant phenotype. Expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^en-e16E results in overgrowth of the wing disc and defects restricted to the posterior compartment of the wing.

(Haerry et al., 1998)

Flies expressing gbb^Scer\UAS.cKa under the control of Scer\GAL4^ptc-559.1 show ectopic vein material anterior to the anterior/posterior boundary in the wing, associated with a decrease in intervein tissue between veins L3 and L4. Flies expressing gbb^Scer\UAS.cKa under the control of Scer\GAL4^71B have wing blisters and ectopic vein material. Flies expressing gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 show excessive vein formation in the wing.

(Khalsa et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, enhanceable by sax⁴/sax[+]

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, enhanceable by Scer\GAL4^A9/tkv^UAS.Tag:HA

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, enhanceable by Df(2R)H23/+

(Bangi and Wharton, 2006)

NOT Enhanced by

Statement

Reference

Scer\GAL4^ptc-559.1, gbb^UAS.cKa has visible phenotype, non-enhanceable by Scer\GAL4^ptc-559.1/kek4^UAS.GFP

(Evans et al., 2009)

Suppressed by

Statement

Reference

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, suppressible | partially by Scer\GAL4^A9/sax^UAS.Tag:FLAG

(Le and Wharton, 2012)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, suppressible by Hsap\ACVR1^UAS.Tag:FLAG, Scer\GAL4^A9

(Le and Wharton, 2012)

Scer\GAL4^ptc-559.1, gbb^UAS.cKa has visible phenotype, suppressible by Scer\GAL4^ptc-559.1/sog^UAS.cYa

(Kang and Bier, 2010)

Scer\GAL4^ptc-559.1, gbb^UAS.cKa has visible phenotype, suppressible by Scer\GAL4^ptc-559.1, gbb^UAS.cKa

(Kang and Bier, 2010)

Scer\GAL4^ptc-559.1, gbb^UAS.cKa has visible phenotype, suppressible by Scer\GAL4^ptc-559.1/kek5^UAS.GFP

(Evans et al., 2009)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, suppressible by Scer\GAL4^A9/cv-2^UAS.cSa

(Serpe et al., 2008)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, suppressible | partially by Scer\GAL4^A9/sax^UAS.Tag:HA

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, suppressible | partially by tkv⁷/tkv[+]

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, suppressible by tkv⁵/tkv[+]

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, suppressible by tkv⁸/tkv[+]

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, suppressible by put[+]/put¹³⁵

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, suppressible by Mad¹²/Mad[+]

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, suppressible by Med[+]/Med¹

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, suppressible by shn^3.3/shn[+]

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, suppressible by Scer\GAL4^A9/sog^UAS.cYa

(Yu et al., 2000)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, suppressible by Scer\GAL4^A9/sog^{supersog1.UAS}

(Yu et al., 2000)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, suppressible | partially by Scer\GAL4^A9/tkv^1ΔGSK.UAS

(Haerry et al., 1998)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, suppressible by Scer\GAL4^A9/sax^ΔI.UAS

(Haerry et al., 1998)

Scer\GAL4^ptc-559.1, gbb^UAS.cKa has visible phenotype, suppressible | partially by dpp^UAS.cHa/Scer\GAL4^ptc-559.1

(Khalsa et al., 1998)

Scer\GAL4^71B, gbb^UAS.cKa has visible phenotype, suppressible | partially by dpp^UAS.cHa/Scer\GAL4^71B

(Khalsa et al., 1998)

Scer\GAL4^A9, gbb^UAS.cKa has visible phenotype, suppressible | partially by dpp^UAS.cHa/Scer\GAL4^A9

(Khalsa et al., 1998)

NOT suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, gbb^UAS.cKa has visible phenotype, non-suppressible by Scer\GAL4^ptc-559.1/kek4^UAS.GFP

(Evans et al., 2009)

Suppressor of

Statement

Reference

Scer\GAL4^how-24B/gbb^UAS.cKa is a suppressor of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^how-24B, frac^UAS.cMa

(Miller et al., 2011)

Scer\GAL4^ptc-559.1, gbb^UAS.cKa is a suppressor of visible phenotype of Scer\GAL4^ptc-559.1, gbb^UAS.cKa

(Kang and Bier, 2010)

gbb^UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of abnormal neuroanatomy phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Saja et al., 2010)

NOT Suppressor of

Statement

Reference

gbb^UAS.cKa/Scer\GAL4^elav.PLu is a non-suppressor of abnormal neuroanatomy phenotype of Neto¹⁰⁹

(Sulkowski et al., 2014)

Scer\GAL4^G14/gbb^UAS.cKa is a non-suppressor of abnormal neuroanatomy phenotype of Neto¹⁰⁹

(Sulkowski et al., 2014)

gbb^UAS.cKa, Scer\GAL4^A9 is a non-suppressor of lethal phenotype of Fs^UAS.cPa, Scer\GAL4^A9

(Pentek et al., 2009)

gbb^UAS.cKa/Scer\GAL4^eve.RN2 is a non-suppressor of abnormal neurophysiology | heat sensitive phenotype of ChAT^ts2

(Fradkin et al., 2008)

Other

Statement

Reference

gbb¹, gbb^UAS.cKa has abnormal neurophysiology | heat sensitive phenotype

(Baines, 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^A9, gbb^UAS.cKa has wing vein phenotype, enhanceable by sax⁴/sax[+]

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has wing vein phenotype, enhanceable by Scer\GAL4^A9/tkv^UAS.Tag:HA

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has wing vein phenotype, enhanceable by Df(2R)H23/+

(Bangi and Wharton, 2006)

NOT Enhanced by

Statement

Reference

Scer\GAL4^ptc-559.1, gbb^UAS.cKa has anterior crossvein phenotype, non-enhanceable by Scer\GAL4^ptc-559.1/kek4^UAS.GFP

(Evans et al., 2009)

Suppressed by

Statement

Reference

Scer\GAL4^A9, gbb^UAS.cKa has wing phenotype, suppressible | partially by Scer\GAL4^A9/sax^UAS.Tag:FLAG

(Le and Wharton, 2012)

Scer\GAL4^A9, gbb^UAS.cKa has wing phenotype, suppressible by Hsap\ACVR1^UAS.Tag:FLAG, Scer\GAL4^A9

(Le and Wharton, 2012)

Scer\GAL4^Toll-6-D42, gbb^UAS.cKa has type I bouton | increased number | third instar larval stage phenotype, suppressible by cmpy^UAS.cJa/Scer\GAL4^Toll-6-D42

(James and Broihier, 2011)

Scer\GAL4^RapGAP1-OK6, gbb^UAS.cKa has type I bouton | increased number | third instar larval stage phenotype, suppressible by cmpy^UAS.cJa/Scer\GAL4^RapGAP1-OK6

(James and Broihier, 2011)

Scer\GAL4^A9, gbb^UAS.cKa has wing phenotype, suppressible | partially by cmpy^UAS.cJa/Scer\GAL4^A9

(James and Broihier, 2011)

Scer\GAL4^ptc-559.1, gbb^UAS.cKa has wing vein | ectopic phenotype, suppressible by Scer\GAL4^ptc-559.1/sog^UAS.cYa

(Kang and Bier, 2010)

Scer\GAL4^ptc-559.1, gbb^UAS.cKa has wing vein | ectopic phenotype, suppressible by Scer\GAL4^ptc-559.1, gbb^UAS.cKa

(Kang and Bier, 2010)

Scer\GAL4^ptc-559.1, gbb^UAS.cKa has anterior crossvein phenotype, suppressible by Scer\GAL4^ptc-559.1/kek5^UAS.GFP

(Evans et al., 2009)

Scer\GAL4^A9, gbb^UAS.cKa has wing phenotype, suppressible by Scer\GAL4^A9/cv-2^UAS.cSa

(Serpe et al., 2008)

Scer\GAL4^A9, gbb^UAS.cKa has wing vein phenotype, suppressible | partially by Scer\GAL4^A9/sax^UAS.Tag:HA

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has wing vein phenotype, suppressible | partially by tkv⁷/tkv[+]

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has wing vein phenotype, suppressible by tkv⁵/tkv[+]

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has wing vein phenotype, suppressible by tkv⁸/tkv[+]

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has wing vein phenotype, suppressible by put[+]/put¹³⁵

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has wing vein phenotype, suppressible by Mad¹²/Mad[+]

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has wing vein phenotype, suppressible by Med[+]/Med¹

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has wing vein phenotype, suppressible by shn^3.3/shn[+]

(Bangi and Wharton, 2006)

Scer\GAL4^A9, gbb^UAS.cKa has wing phenotype, suppressible by Scer\GAL4^A9/tsg^UAS.cYa

(Vilmos et al., 2005)

Scer\GAL4^A9, gbb^UAS.cKa has wing phenotype, suppressible by Scer\GAL4^A9/sog^UAS.cYa/tsg^UAS.cYa

(Vilmos et al., 2005)

Scer\GAL4^A9, gbb^UAS.cKa has wing cell phenotype, suppressible by Scer\GAL4^A9/sog^UAS.cYa

(Yu et al., 2000)

Scer\GAL4^A9, gbb^UAS.cKa has wing vein | ectopic phenotype, suppressible by Scer\GAL4^A9/sog^UAS.cYa

(Yu et al., 2000)

Scer\GAL4^A9, gbb^UAS.cKa has wing phenotype, suppressible by Scer\GAL4^A9/sog^UAS.cYa

(Yu et al., 2000)

Scer\GAL4^A9, gbb^UAS.cKa has wing phenotype, suppressible by Scer\GAL4^A9/sog^{supersog1.UAS}

(Yu et al., 2000)

Scer\GAL4^A9, gbb^UAS.cKa has wing phenotype, suppressible | partially by Scer\GAL4^A9/tkv^1ΔGSK.UAS

(Haerry et al., 1998)

Scer\GAL4^A9, gbb^UAS.cKa has wing phenotype, suppressible by Scer\GAL4^A9/sax^ΔI.UAS

(Haerry et al., 1998)

Scer\GAL4^ptc-559.1, gbb^UAS.cKa has wing vein phenotype, suppressible | partially by dpp^UAS.cHa/Scer\GAL4^ptc-559.1

(Khalsa et al., 1998)

Scer\GAL4^ptc-559.1, gbb^UAS.cKa has wing phenotype, suppressible | partially by dpp^UAS.cHa/Scer\GAL4^ptc-559.1

(Khalsa et al., 1998)

Scer\GAL4^71B, gbb^UAS.cKa has wing vein phenotype, suppressible | partially by dpp^UAS.cHa/Scer\GAL4^71B

(Khalsa et al., 1998)

Scer\GAL4^71B, gbb^UAS.cKa has wing phenotype, suppressible | partially by dpp^UAS.cHa/Scer\GAL4^71B

(Khalsa et al., 1998)

Scer\GAL4^A9, gbb^UAS.cKa has wing vein phenotype, suppressible | partially by dpp^UAS.cHa/Scer\GAL4^A9

(Khalsa et al., 1998)

Scer\GAL4^A9, gbb^UAS.cKa has wing phenotype, suppressible | partially by dpp^UAS.cHa/Scer\GAL4^A9

(Khalsa et al., 1998)

NOT suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, gbb^UAS.cKa has anterior crossvein phenotype, non-suppressible by Scer\GAL4^ptc-559.1/kek4^UAS.GFP

(Evans et al., 2009)

Scer\GAL4^A9, gbb^UAS.cKa has wing phenotype, non-suppressible by Scer\GAL4^A9/cv^UAS.cVa

(Vilmos et al., 2005)

Scer\GAL4^A9, gbb^UAS.cKa has wing vein phenotype, non-suppressible by Xlae\noggin^UAS.cYa/Scer\GAL4^A9

(Yu et al., 2000)

Suppressor of

Statement

Reference

Scer\GAL4^how-24B/gbb^UAS.cKa is a suppressor of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of Scer\GAL4^how-24B, frac^UAS.cMa

(Miller et al., 2011)

Scer\GAL4^ptc-559.1, gbb^UAS.cKa is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^ptc-559.1, gbb^UAS.cKa

(Kang and Bier, 2010)

gbb^UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Saja et al., 2010)

gbb^UAS.cKa/Scer\GAL4^ap-md544 is a suppressor of larval ventral nerve cord phenotype of Scer\GAL4^ap-md544, robo1^UAS.cKa

(Allan et al., 2003)

gbb^UAS.cKa/Scer\GAL4^ap-md544 is a suppressor of embryonic/larval neuron phenotype of Scer\GAL4^ap-md544, robo1^UAS.cKa

(Allan et al., 2003)

NOT Suppressor of

Statement

Reference

gbb^UAS.cKa/Scer\GAL4^elav.PLu is a non-suppressor of terminal bouton phenotype of Neto¹⁰⁹

(Sulkowski et al., 2014)

Scer\GAL4^G14/gbb^UAS.cKa is a non-suppressor of terminal bouton phenotype of Neto¹⁰⁹

(Sulkowski et al., 2014)

Other

Statement

Reference

Hsap\ACVR1^UAS.Tag:FLAG, Scer\GAL4^A9, gbb^UAS.cKa has wing vein phenotype

(Le and Wharton, 2012)

Scer\GAL4^A9, cv^UAS.cVa, gbb^UAS.cKa, sog^UAS.cYa has crossvein phenotype

(Vilmos et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The ability of gbb^Scer\UAS.cKa expressed under the control of Scer\GAL4^Toll-6-D42 to rescue the electrophysiological defects seen at the neuromuscular junction in gbb¹/gbb² larvae is impaired but not completely abolished if the larvae are also homozygous for cmpy^Δ8.

(James et al., 2014)

Expression of gbb^Scer\UAS.cKa under the control of either Scer\GAL4^elav.PLu or Scer\GAL4^G14 does not suppress the reduction in NMJ bouton number seen in Neto¹⁰⁹ mutant larvae.

(Sulkowski et al., 2014)

The severity of the wing phenotype caused by expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 is reduced by co-expression of sax^{Scer\UAS.T:Zzzz\FLAG}.

(Le and Wharton, 2012)

Co-expression of cmpy^Scer\UAS.cJa suppresses the neuromuscular overgrowth phenotype caused by expression of gbb^Scer\UAS.cKa using either Scer\GAL4^D42 or Scer\GAL4^OK6.

Co-expression of cmpy^Scer\UAS.cJa suppresses the wing blistering phenotype caused by Scer\GAL4^A9-mediated expression of gbb^Scer\UAS.cKa in males and females.

(James and Broihier, 2011)

Mesodermal expression of gbb^Scer\UAS.cKa (under the control of Scer\GAL4^how-24B) suppresses the phenotype observed with frac^Scer\UAS.cMa over-expression (48% to 28%).

(Miller et al., 2011)

Co-expression of either sog^Scer\UAS.cYa or Hip14^Scer\UAS.cKa suppresses the ectopic wing vein phenotype caused by expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^ptc-559.1.

(Kang and Bier, 2010)

Overexression of gbb^Scer\UAS.cKa with Ppt1^Scer\UAS.cKa, both under the control of Scer\GAL4^GMR.PF suppresses the visual system degeneration seen upon Ppt1^Scer\UAS.cKa expression alone.

(Saja et al., 2010)

The ectopic vein phenotype in the area where the anterior crossvein normally forms resulting from the Scer\GAL4^ptc-559.1 driven expression of gbb^Scer\UAS.cKa can be suppressed by co-expressing kek5^{Scer\UAS.T:Avic\GFP} in the wing.

The ectopic vein phenotype in the area where the anterior crossvein normally forms resulting from the Scer\GAL4^ptc-559.1 driven expression of gbb^Scer\UAS.cKa is not influenced by co-expressing kek4^{Scer\UAS.T:Avic\GFP} in the wing.

(Evans et al., 2009)

Overexpression of gbb^Scer\UAS.cKa post-synaptically under the control of Scer\GAL4^eve.RN2 fails to suppress the reduced synaptic currents found in Cha^ts2 mutant second instar larvae raised at 18[o]C.

(Fradkin et al., 2008)

Expression of cv-2^Scer\UAS.cSa suppresses the wing phenotypes seen when gbb^Scer\UAS.cKa is expressed under the control of Scer\GAL4^A9.

(Serpe et al., 2008)

Coexpression of gbb^Scer\UAS.cKa in the wing disc with sax^{Scer\UAS.T:Ivir\HA1}, under the control of Scer\GAL4^A9, results in an enhancement of the Scer\GAL4^A9>gbb^Scer\UAS.cKa wing vein phenotype; all wings show the mildest form of vein phenotype in the gbb^Scer\UAS.cKa-sax^{Scer\UAS.T:Ivir\HA1} coexpressing wings, while less than 10% of wings show this mild phenotype in gbb^Scer\UAS.cKa wings.

Expression of gbb^Scer\UAS.cKa, under the control of Scer\GAL4^A9, in a sax⁴/+ background partially suppresses the extra wing vein phenotype; over 90% of flies expressing both UAS transgenes show the most severe form of the Scer\GAL4^A9>gbb^Scer\UAS.cKa phenotype, compared to about 20% of Scer\GAL4^A9>gbb^Scer\UAS.cKa flies.

Coexpression of tkv^{Scer\UAS.T:Ivir\HA1} and gbb^Scer\UAS.cKa enhances the Scer\GAL4^A9>gbb^Scer\UAS.cKa wing vein phenotype so that a higher percentage of wings have extensive amounts of wing vein tissue.

Expression of gbb^Scer\UAS.cKa, under the control of Scer\GAL4^A9, in a tkv⁷/+ background suppresses the extra wing vein phenotype of Scer\GAL4^A9>gbb^Scer\UAS.cKa flies. Around 80% of Scer\GAL4^A9>gbb^Scer\UAS.cKa; tkv⁷/+ wings show a very mild vein phenotype, while around 20% appear wild type.

(Bangi and Wharton, 2006)

Co-expression of cv^Scer\UAS.cVa with gbb^Scer\UAS.cKa, under the regulation of Scer\GAL4^A9 has no detectable effect on the overexpression phenotype of gbb^Scer\UAS.cKa (wing disruption). Co-expression of cv^Scer\UAS.cVa and sog^Scer\UAS.cYa, with gbb^Scer\UAS.cKa, under the regulation of Scer\GAL4^A9 re-establishes the intervein tissue and the longitudinal veins with a series of expanded crossveins between L2 and L3 on the wing. Therefore, cv and sog interact together to suppress the wing phenotype of gbb. Co-expression of tsg^Scer\UAS.cYa and sog^Scer\UAS.cYa, along with gbb^Scer\UAS.cKa, under the regulation of Scer\GAL4^A9 partially suppresses the excess gbb^Scer\UAS.cKa wing disruption phenotype. Co-expression of tsg^Scer\UAS.cYa and gbb^Scer\UAS.cKa, under the regulation of Scer\GAL4^A9 suppresses the excess gbb phenotype.

(Vilmos et al., 2005)

Postsynaptic expression of gbb^Scer\UAS.cKa, under the control of Scer\GAL4^eve.RN2, fails to potentiate synaptic current amplitude in aCC/RP2 neurons when expressed in a Cha^ts2 homozygous background at 29^oC, compared to in a Cha^ts2/+ control background. If larvae are allowed to recover at 20^oC for another 22 hours, the ability of gbb^Scer\UAS.cKa to potentiate synaptic current amplitude in a Cha^ts2 background is restored.

(Baines, 2004)

The solid vein phenotype caused by expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 is strongly suppressed by co-expression of sog^Scer\UAS.cYa or of sog^{supersog1.Scer\UAS}.

(Yu et al., 2000)

Flies expressing gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 at 25^oC have slightly larger wings than normal with minor venation defects along veins L2 and L5. Flies expressing gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 at 30^oC have blistered and highly pigmented wings. The wing phenotype caused by expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 is partially suppressed if the flies are also coexpressing tkv^{1ΔGSK.Scer\UAS}, and is almost completely suppressed if the flies are also coexpressing sax^ΔI.Scer\UAS.

(Haerry et al., 1998)

Xenogenetic Interactions

Statement

Reference

The wing phenotype caused by expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 is suppressed by co-expression of Hsap\ACVR1^{Scer\UAS.T:Zzzz\FLAG} and the resulting flies have a reduction in wing size and a loss of wing vein material.

(Le and Wharton, 2012)

The solid vein phenotype caused by expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 is not suppressed by co-expression of Xlae\noggin^Scer\UAS.cYa.

(Yu et al., 2000)

Complementation and Rescue Data

Rescues

gbb^UAS.cKa/Scer\GAL4^Toll-6-D42 rescues gbb²/gbb¹

(James et al., 2014)

Scer\GAL4^fat/gbb^UAS.cKa rescues gbb¹

(Ballard et al., 2010)

Scer\GAL4^{VP16.nanos.UTR}/gbb^UAS.cKa rescues gbb⁴

(Shivdasani and Ingham, 2003)

Partially rescues

Scer\GAL4^BG380/gbb^UAS.cKa partially rescues gbb²/gbb¹

(McCabe et al., 2003)

Scer\GAL4^G14/gbb^UAS.cKa partially rescues gbb²/gbb¹

(McCabe et al., 2003)

Scer\GAL4^elav-C155/gbb^UAS.cKa partially rescues gbb²/gbb¹

(McCabe et al., 2003)

gbb^UAS.cKa partially rescues gbb²/gbb¹

(McCabe et al., 2003)

Scer\GAL4^how-24B/gbb^UAS.cKa partially rescues gbb⁴/gbb¹

(Khalsa et al., 1998)

gbb^UAS.cKa/Scer\GAL4^hs.PB partially rescues gbb⁴/gbb¹

(Khalsa et al., 1998)

Comments

Expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^Toll-6-D42 rescues the reduction in spontaneous miniature excitatory junction potential frequency, evoked excitatory junction potential amplitude and quantal content which is seen at the neuromuscular junction in gbb¹/gbb² larvae.

(James et al., 2014)

The phenotypes associated with homozygous gbb¹ mutants (including lethality, larval transparency and abnormal fatty acid uptake and transport to the fat body) are rescued by expression of gbb^Scer\UAS.cKa driven by Scer\GAL4^fat.

(Ballard et al., 2010)

The presence of P{UAS-gbb.K}9.9 alone (in the absence of any driver) can rescue survival of gbb¹/gbb² animals to 3rd instar or pupal stage. The additional presence of Scer\GAL4^G14 rescues bouton density and spontaneous mini-EJP (excitatory junctional potential) frequency to wild-type levels, and partially rescues spontaneous mini-EJP amplitude, evoked EJP frequency and levels of neurotransmitter release at synapses. Essentially identical results are seen when Scer\GAL4^BG380 is used instead (although bouton density not measured). However, when Scer\GAL4^elav-C155 is used there is only a slight rescue of bouton density, but the frequency and amplitude of spontaneous mini-EJPs, the amplitude of evoked EJPs and the levels of neurotransmitter release at synapses are all rescued to wild-type levels.

(McCabe et al., 2003)

The testis defects seen in gbb⁴ homozygous males are rescued by expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Shivdasani and Ingham, 2003)

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Wild-type

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Notes on Origin

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Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

gbb^Scer\UAS.cKa

gbb^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Khalsa

(Khalsa et al., 1998)

Secondary FlyBase IDs

References (40)

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