FlyBase Allele Report: Dmel\Src64B[PI]

Local transposition of P{lwB.D}BGT-T063-w.mut; the original P{lwB.D}BGT-T063-w.mut insertion remains, and a second insertion (P{lwB.D}Src64B^PI) is present in the second intron, approximately 2.5kb downstream of the original insertion.

(Dodson et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Nicolai et al., 2003)

female semi-sterile | recessive

(Djagaeva, 2005, Dodson et al., 1998)

male fertile

(Dodson et al., 1998)

viable

(Djagaeva, 2005, Nicolai et al., 2003, Jackson and Berg, 2002, Dodson et al., 1998)

Phenotype Manifest In

adult mushroom body

(Nicolai et al., 2003)

alpha-lobe & axon

(Nicolai et al., 2003)

beta-lobe & axon

(Nicolai et al., 2003)

egg

(Dodson et al., 1998)

egg chamber

(Djagaeva, 2005, Dodson et al., 1998)

egg chamber | germline clone

(Djagaeva, 2005)

inner nurse cell ring canal rim

(Jackson and Berg, 2002)

nurse cell

(Takahashi et al., 2005, Dodson et al., 1998)

nurse cell ring canal

(Dodson et al., 1998)

outer nurse cell ring canal rim

(Jackson and Berg, 2002)

presumptive embryonic salivary gland

(Harris and Beckendorf, 2007)

Detailed Description

Statement

Reference

Mutant embryos have salivary glands that curve ventrally at the tip, instead of lying parallel to the midline as in wild-type embryos.

(Harris and Beckendorf, 2007)

Src64B^PI embryos show no salivary gland abnormalities.

(Chandrasekaran and Beckendorf, 2005)

After several generations, the fertility of Src64B^PI homozygous lines increases and the number and severity of egg chamber defects decreases. In the ovarioles of Src64B^PI mutants, 25% contain fused egg chambers at 18^oC; this value drops to 15% at 25^oC. Germline clones for Src64B^PI have similar morphology (18^oC - 30%).

(Djagaeva, 2005)

Ovaries of Src64B^PI mutants show nurse cell fusions.

(Takahashi et al., 2005)

Src64B^PI is homozygous viable and shows a clear mushroom body phenotype. The beta lobes fuse, the alpha axons are reduced in number and both sets of axons show defasciculation. When analyzed in clones in the mushroom body, the axons of the beta-lobe cross the interhemispheric boundary. This phenotype is more penetrant when Src64B^PI is hemizygous than when it is homozygous.

(Nicolai et al., 2003)

The diameters of the inner and outer rims of the ring canals are smaller than normal in homozygous females.

(Jackson and Berg, 2002)

Homozygous females have reduced fertility; eggs laid by these females hatch at a reduced frequency (5.5%) compared to wild-type. Egg chambers of homozygous females show a defect in cytoplasmic transfer; in contrast to wild-type, 55% of late stage homozygous egg chambers have nurse cell cytoplasm remaining at the anterior end of the oocyte. Eggs from these females range in length from 50-100% wild-type egg length. Ring canals are smaller than wild-type, and show defects in growth during both early and late stages of their morphogenesis. Approximately 70% of stage 9-10 egg chambers show fusions between nurse cells. 45% of stage 10A egg chambers contain fewer than 15 ring canals.

(Dodson et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Src64B^PI has abnormal neuroanatomy phenotype, enhanceable by Df(2R)lio²

(Nicolai et al., 2003)

Enhancer of

Statement

Reference

Src64B[+]/Src64B^PI is an enhancer of embryonic/larval segmentation phenotype phenotype of Src42A^26-1/Src42A[+], Stat92E⁰⁶³⁴⁶

(Sotillos et al., 2013)

Src42A^E1, Src42A[+], Src64B[+], Src64B^PI is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Fak^K24/Fak^N30

(Tsai et al., 2008)

Suppressor of

Statement

Reference

Src64B^PI is a suppressor of visible phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

Src64B^PI is a suppressor of visible phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

Src64B^PI is a suppressor of lethal | pupal stage phenotype of Csk^j1D8/Csk^S030003

(Read et al., 2004)

Src64B[+]/Src64B^PI is a suppressor of abnormal neuroanatomy phenotype of RhoGAPp190^RNAi.GAP.UAS, Scer\GAL4^ey-OK107

(Billuart et al., 2001)

Other

Statement

Reference

Src42A^E1/Src42A[+], Src64B^PI has abnormal neuroanatomy | third instar larval stage phenotype

(Tsai et al., 2008)

Fak^N30/Fak^KG00304, Src64B[+]/Src64B^PI has abnormal neuroanatomy | third instar larval stage phenotype

(Tsai et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Src64B^PI has egg chamber phenotype, enhanceable by Btk29A[+]/Btk^k05610

(Djagaeva, 2005)

Src64B^PI has beta-lobe & axon phenotype, enhanceable by Df(2R)lio²

(Nicolai et al., 2003)

Src64B^PI has mushroom body phenotype, enhanceable by Df(2R)lio²

(Nicolai et al., 2003)

NOT Enhanced by

Statement

Reference

Src64B^PI has nurse cell phenotype, non-enhanceable by Src42A^26-1

(Takahashi et al., 2005)

Suppressed by

Statement

Reference

Src64B^PI has outer nurse cell ring canal rim phenotype, suppressible by Akap200^k07118a/Akap200[+]

(Jackson and Berg, 2002)

Src64B^PI has inner nurse cell ring canal rim phenotype, suppressible by Akap200^k07118a/Akap200[+]

(Jackson and Berg, 2002)

Enhancer of

Statement

Reference

Src64B[+]/Src64B^PI is an enhancer of embryonic/larval spiracle phenotype of Src42A^26-1/Src42A[+], Stat92E⁰⁶³⁴⁶

(Sotillos et al., 2013)

Src64B[+]/Src64B^PI is an enhancer of embryonic head phenotype of Src42A^26-1/Src42A[+], Stat92E⁰⁶³⁴⁶

(Sotillos et al., 2013)

Src64B^PI is an enhancer of embryonic/larval dorsal tracheal branch | embryonic stage phenotype of Src42A^myri

(Shindo et al., 2008)

Src42A^E1, Src42A[+], Src64B[+], Src64B^PI is an enhancer of neuromuscular junction | third instar larval stage phenotype of Fak^K24/Fak^N30

(Tsai et al., 2008)

Src64B^PI is an enhancer of presumptive embryonic salivary gland phenotype of Btk^k00206

(Chandrasekaran and Beckendorf, 2005)

Src64B[+]/Src64B^PI is an enhancer of embryo | dorsal closure stage phenotype of Src42A^26-1

(Takahashi et al., 2005)

Suppressor of

Statement

Reference

Src64B^PI is a suppressor of eye phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

Src64B^PI is a suppressor of eye phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

Src64B[+]/Src64B^PI is a suppressor of mushroom body vertical lobe phenotype of RhoGAPp190^RNAi.GAP.UAS, Scer\GAL4^ey-OK107

(Billuart et al., 2001)

Other

Statement

Reference

Src42A^E1/Src42A[+], Src64B^PI has neuromuscular junction | third instar larval stage phenotype

(Tsai et al., 2008)

Fak^N30/Fak^KG00304, Src64B[+]/Src64B^PI has neuromuscular junction | third instar larval stage phenotype

(Tsai et al., 2008)

Fak^N30/Fak^KG00304, Src64B[+]/Src64B^PI has NMJ bouton | increased number | third instar larval stage phenotype

(Tsai et al., 2008)

Btk^k00206, Src64B^PI has embryonic head segment phenotype

(Chandrasekaran and Beckendorf, 2005)

Src42A^26-1, Src64B[+]/Src64B^PI has embryonic leading edge cell phenotype

(Takahashi et al., 2005)

Src42A^26-1, Src64B^PI has tracheal branch primordium phenotype

(Takahashi et al., 2005)

Src42A^26-1, Src64B[+]/Src64B^PI has embryonic head phenotype

(Takahashi et al., 2005)

Src42A^26-1, Src64B[+]/Src64B^PI has Bolwig organ phenotype

(Takahashi et al., 2005)

Src42A^26-1, Src64B[+]/Src64B^PI has tracheal branch primordium phenotype

(Takahashi et al., 2005)

Src42A^26-1, Src64B^PI has mitotic domain 1 | extended germ band stage phenotype

(Takahashi et al., 2005)

Src42A^26-1, Src64B^PI has embryonic leading edge cell phenotype

(Takahashi et al., 2005)

Src42A^26-1, Src64B^PI has embryonic head phenotype

(Takahashi et al., 2005)

Src42A^26-1, Src64B^PI has Bolwig organ phenotype

(Takahashi et al., 2005)

Src42A^myri, Src64B^PI has embryonic/first instar larval cuticle | dorsal phenotype

(Tateno et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Src42A^26-1/+ ; Src64B^PI/+ double heterozygous embryos have normal cuticles.

Src42A^26-1/+ ; Stat92E⁰⁶³⁴⁶/+ double heterozygous embryos show defects in head formation and have segmentation and spiracle defects. This phenotype is enhanced if the embryos are also heterozygous for Src64B^PI.

(Sotillos et al., 2013)

Src64B^PI enhances the tracheal defects seen in Src42A^myri mutant embryos. Many dorsal branches fail to extend correctly, remaining in a multicellular state despite the progression of dorsal closure.

(Shindo et al., 2008)

Significant overgrowth of the neuromuscular junction (NMJ) (increased bouton number per muscle area and increased NMJ length per muscle area) is seen in Src64B^PI/+ Fak^N30/Fak^KG00304 third instar larvae.

Significant overgrowth of the NMJ (increased bouton number per muscle area) is seen in Src42A^E1/+ ; Src64B^PI/Src64B^PI third instar larvae.

The overgrowth at the NMJ (increased bouton number per muscle area) seen in Fak^N30/Fak^K24 third instar larvae is enhanced if they are also carrying both Src42A^E1/+ and Src64B^PI/+.

(Tsai et al., 2008)

drl^R343 ; Src64B^PI double heterozygotes show salivary gland guidance defects; the tips of the salivary glands curve ventromedially towards the central nervous system, instead of lying parallel to the midline as in wild-type embryos.

The salivary glands of drl^R343 ; Src64B^PI double homozygous embryos show a similar frequency of guidance defects as that seen in either of the single mutants alone.

(Harris and Beckendorf, 2007)

Btk29A^k00206; Src64B^PI double mutant embryos suffer gross abnormalities, such as lack of head segments. Btk29A^k00206/+; Src64B^PI/+ double heterozygotes have no salivary gland defects, while around one third of Btk29A^k00206/+; Src64B^PI double mutants show invagination defects.

(Chandrasekaran and Beckendorf, 2005)

Reducing the Btk29A dose in Src64B^PI homozygotes enhances the defective ovariole phenotype: 70% of ovarioles contain fused egg chambers in Btk29A^k05610/+; Src64B^PI mutants.

(Djagaeva, 2005)

34% of Src42A^26-1; Src64B^PI/+ embryos show a dorsal open phenotype and head involution defects. These embryos have misrouted longitudinal axons, but appear to have a normal number of neurons. The formation of Bolwig's organ and the trachea is significantly disrupted. Src42A^26-1; Src64B^PI embryos show more severe phenotypes than Src42A^26-1; Src64B^PI/+ embryos, with no germband retraction. The ovaries of Src42A^26-1/+; Src64B^PI/+ mutants show no nurse cell fusions. The nurse cell phenotype of Src64B^PI ovaries is not enhanced in Src42A^26-1; Src64B^PI double mutants. Src42A^26-1; Src64B^PI/+ embryos exhibit almost normal cell-shape change at 9 hours AEL, but at later stages the leading edge is frequently kinked with partial dorsal-most epidermal cell deformation.

(Takahashi et al., 2005)

The small ring canal phenotype see in Src64B^PI homozygous females is dominantly suppressed by Akap200^k07118a.

(Jackson and Berg, 2002)

Src64B^PI capt¹⁰ double heterozygotes do not show significant midline crossing defects in the embryonic central nervous system.

(Wills et al., 2002)

Src42A^myri; Src64B^PI double homozygous embryos have a mild but clear dorsal open phenotype.

(Tateno et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Src64B^Δ17

(Dodson et al., 1998)

Partially rescued by

Src64B^PI is partially rescued by Src64B^αTub67C.PD

(Dodson et al., 1998)

Comments

Does not complement the cytoplasmic transfer defect of Src64B^Δ17.

(Dodson et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7379

y¹ w^*; P{lwB.D}BGT-T063-w.mut P{lwB.D}Src64B^PI

Notes on Origin

Discoverer

Comments

The reduction in female fertility and the defect in cytoplasmic transfer are reverted by excision of the P{lwB.D} element in the second intron.

(Dodson et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Src64^P1

(Harris and Beckendorf, 2007, Nicolai et al., 2003, Tateno et al., 2000)

Src64^PI

(Billuart et al., 2001)

Src64B^P1

(Sotillos et al., 2013, Shindo et al., 2008, Djagaeva, 2005, Djagaeva et al., 2005, Read et al., 2005, Takahashi et al., 2005)

Src64B^PI

(Tsai et al., 2008)

src64^P1

(Wills et al., 2002)

src64BP1

(Alajati et al., 2020)

Name Synonyms

Secondary FlyBase IDs

FBal0103951

References (17)

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