FlyBase Allele Report: Dmel\N[int.SH.UAS]

General Information

Symbol

Dmel\N^int.SH.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0093233

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{UAS-N.intra.SH}

Also Known As

UAS-N^intra, UAS-Nintra, UAS-Notch^intra, Nintra, UAS-N^icd

Key Links

Transgenic product class

inframe_deletion

(Zecchini et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Zecchini et al., 1999)

Mutagen

in vitro construct

(Klein and Martinez Arias, 1998)

Progenitor genotype

Carried in construct

P{UAS-N.intra.SH}

Cytology

Description

The extracellular and transmembrane domains of N are deleted but the DNA coding for the intracellular domain is kept intact.

(Zecchini et al., 1999)

UAS regulatory sequences drive expression of a constitutively active form of N.

(Klein and Martinez Arias, 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^insc.PU

(Bivik et al., 2016)

decreased occurrence of cell division | embryonic stage, with Scer\GAL4^GMR42G10

(Bivik et al., 2016)

lethal, with Scer\GAL4^klu-G410

(Bishop et al., 1999)

visible, with Scer\GAL4^ap-md544

(Bishop et al., 1999)

visible, with Scer\GAL4^sca-P309

(Reddy and Rodrigues, 1999)

Phenotype Manifest In

chemosensory sensory organ & wing vein L1 & glial cell, with Scer\GAL4^hs.PB

(van de Bor and Giangrande, 2001)

chemosensory sensory organ & wing vein L3 & glial cell, with Scer\GAL4^hs.PB

(van de Bor and Giangrande, 2001)

dorsal mesothoracic disc & filamentous actin | somatic clone, with Scer\GAL4^Act5C.PI

(Major and Irvine, 2005)

eo neuron, with Scer\GAL4^hs.PB

(van de Bor and Giangrande, 2001)

external sensory organ precursor cell IIa, with Scer\GAL4^hs.PB

(van de Bor and Giangrande, 2001)

external sensory organ precursor cell IIb, with Scer\GAL4^hs.PB

(van de Bor and Giangrande, 2001)

eye, with Scer\GAL4^ey.PH

(Dominguez and de Celis, 1998)

mechanosensory sensory organ & wing vein L1 & glial cell, with Scer\GAL4^hs.PB

(van de Bor and Giangrande, 2001)

mechanosensory sensory organ & wing vein L3 & glial cell, with Scer\GAL4^hs.PB

(van de Bor and Giangrande, 2001)

muscle founder cell, with Scer\GAL4^da.G32

(Giebel, 1999)

muscle founder cell, with Scer\GAL4^how-24B

(Giebel, 1999)

nervous system, with Scer\GAL4^da.G32

(Giebel et al., 1997)

tarsal segment 4, with Scer\GAL4^ap-md544

(Bishop et al., 1999)

tarsal segment 4 & joint, with Scer\GAL4^ap-md544

(Bishop et al., 1999)

tormogen cell, with Scer\GAL4^hs.PB

(van de Bor and Giangrande, 2001)

tormogen cell, with Scer\GAL4^sca-P309

(Reddy and Rodrigues, 1999)

tormogen cell & antennal segment 3 | conditional ts, with Scer\GAL4^sca-P309

(Sen et al., 2003)

tormogen cell & antennal segment 3 | ectopic | conditional ts, with Scer\GAL4^sca-P309

(Sen et al., 2003)

trichogen cell, with Scer\GAL4^hs.PB

(van de Bor and Giangrande, 2001)

trichogen cell, with Scer\GAL4^sca-P309

(Reddy and Rodrigues, 1999)

trichogen cell & antennal segment 3 | conditional ts, with Scer\GAL4^sca-P309

(Sen et al., 2003)

trichogen cell & antennal segment 3 | ectopic | conditional ts, with Scer\GAL4^sca-P309

(Sen et al., 2003)

visceral mesoderm, with Scer\GAL4^how-24B

(Lawrence et al., 2001)

Detailed Description

Statement

Reference

Expression of N^{int.SH.Scer\UAS} under the control of Scer\GAL4^insc.PU in embryos leads to a significant reduction in thoracic and abdominal neuroblast daughter cell proliferation at stage 12 (but not stage 14), but no significant difference in thoracic or abdominal neuroblast proliferation at stage 12 or 14, as compared to controls.

(Bivik et al., 2016)

Clones of N^{int.SH.Scer\UAS} in the wing disc, driven by Scer\GAL4^Act5C.PI, have no autonomous effect on F-actin. Occasionally, upregulation of F-actin is observed at the clone edges consistent with N ligand expression.

(Major and Irvine, 2005)

When N^{int.SH.Scer\UAS}, Scer\GAL4^sca-P309 animals are kept at 16^oC until 10 h APF, and then shifted to 28^oC (to activate Scer\GAL4^sca-P309 in secondary progenitors of external sensory organs), adult antenna show a variety of defects. Multiple sockets are seen, and sensilla with two shafts arising from a socket.

(Sen et al., 2003)

When N^{int.SH.Scer\UAS} is driven by Scer\GAL4^how-24B in the mesoderm differentiation of the visceral mesoderm is disrupted.

(Lawrence et al., 2001)

When expression is driven by Scer\GAL4^hs.PB at the time of the birth of the sensory organ precursor cells all L3 glia and most L1 glia are absent. Transformations affecting sensory organ precursor IIb (into IIa), the glial precursor (into IIIb) and the neuron (into a thecogen cell) are observed. When expression is driven in non-gliogenic sensory organ lineages, partial or total transformation of neurons in thecogen sheath cells is observed. The strongest defect is a sensory organs of 6 cells, four being thecogen cells.

(van de Bor and Giangrande, 2001)

Expression of N^{int.SH.Scer\UAS} under the control of Scer\GAL4^klu-G410 results in lethality. Expression of N^{int.SH.Scer\UAS} under the control of Scer\GAL4^ap-md544 results in a hyper-jointed fourth tarsal segment; double ball joints with reversed polarity are formed. In addition, the interjoint region is reduced.

(Bishop et al., 1999)

Embryos carrying N^{int.SH.Scer\UAS} driven by Scer\GAL4^da.G32 or Scer\GAL4^how-24B appear to have no muscle founder cells, and fail to differentiate muscle fibres. Embryos carrying N^{int.SH.Scer\UAS} driven by Scer\GAL4^da.G32 have no PNS structures.

(Giebel, 1999)

Flies carrying N^{int.SH.Scer\UAS} expressed under the control of Scer\GAL4^sca-P309 (reared at 18^oC throughout development and raised to 25^oC at the beginning of pupation) show duplication of the tormogen at the expense of the trichogen cell, leading to double sockets. Three-socket and four-socket phenotypes are also seen.

(Reddy and Rodrigues, 1999)

When expression is driven by Scer\GAL4^pnr-MD237 or Scer\GAL4^da.G32 no effect on dorsal closure is seen.

(Zecchini et al., 1999)

Rough eye phenotype.

(Dominguez and de Celis, 1998)

Scer\GAL4^da.G32 mediated expression of N^{int.SH.Scer\UAS} blocks neural development completely.

(Giebel et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

N^int.SH.UAS, Scer\GAL4^da.G32 has peripheral nervous system phenotype, suppressible by H^UAS.cGb, Scer\GAL4^da.G32

(Giebel, 1999)

N^int.SH.UAS, Scer\GAL4^how-24B has muscle founder cell phenotype, suppressible by H^UAS.cGb, Scer\GAL4^how-24B

(Giebel, 1999)

N^int.SH.UAS, Scer\GAL4^how-24B has embryonic/larval somatic muscle cell phenotype, suppressible by H^UAS.cGb, Scer\GAL4^how-24B

(Giebel, 1999)

N^int.SH.UAS, Scer\GAL4^how-24B has visceral muscle fiber phenotype, suppressible by H^UAS.cGb, Scer\GAL4^how-24B

(Giebel, 1999)

N^int.SH.UAS, Scer\GAL4^da.G32 has muscle founder cell phenotype, suppressible by H^UAS.cGb, Scer\GAL4^da.G32

(Giebel, 1999)

N^int.SH.UAS, Scer\GAL4^da.G32 has embryonic/larval somatic muscle cell phenotype, suppressible by H^UAS.cGb, Scer\GAL4^da.G32

(Giebel, 1999)

N^int.SH.UAS, Scer\GAL4^da.G32 has visceral muscle fiber phenotype, suppressible by H^UAS.cGb, Scer\GAL4^da.G32

(Giebel, 1999)

N^int.SH.UAS, Scer\GAL4^da.G32 has central nervous system phenotype, suppressible by H^UAS.cGb, Scer\GAL4^da.G32

(Giebel, 1999)

NOT suppressed by

Statement

Reference

N^int.SH.UAS, Scer\GAL4^da.G32 has muscle founder cell phenotype, non-suppressible by l(1)sc^UAS.cHa/da^UAS.cGa, Scer\GAL4^da.G32

(Giebel, 1999)

Suppressor of

Statement

Reference

N^int.SH.UAS/Scer\GAL4^dpp.blk1 is a suppressor of wing margin phenotype of Psn^I2/Psn^B3

(Klein et al., 2000)

N^int.SH.UAS/Scer\GAL4^dpp.blk1 is a suppressor of wing disc phenotype of Psn^I2/Psn^B3

(Klein et al., 2000)

N^int.SH.UAS, Scer\GAL4^ey.PH is a suppressor | partially of eye phenotype of Scer\GAL4^ey.PH, fng^UAS.cKa

(Dominguez and de Celis, 1998)

N^int.SH.UAS, Scer\GAL4^ey.PH is a suppressor | partially of eye phenotype of Scer\GAL4^ey.PH, caup^UAS.cdCa

(Dominguez and de Celis, 1998)

NOT Suppressor of

Statement

Reference

N^int.SH.UAS/Scer\GAL4^dpp.blk1 is a non-suppressor of wing disc phenotype of Su(H)⁸/Su(H)²

(Klein et al., 2000)

Other

Statement

Reference

N^int.SH.UAS, Scer\GAL4^ey.PH, fng^UAS.cKa has ommatidium phenotype

(Dominguez and de Celis, 1998)

N^int.SH.UAS, Scer\GAL4^ey.PH, caup^UAS.cdCa has ommatidium phenotype

(Dominguez and de Celis, 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of l(1)sc^Scer\UAS.cHa and da^Scer\UAS.cGa does not rescue the mesoderm phenotype of N^{int.SH.Scer\UAS} driven by Scer\GAL4^da.G32.

Co-expression of H^Scer\UAS.cGb and N^{int.SH.Scer\UAS} driven by Scer\GAL4^da.G32 or Scer\GAL4^how-24B produces embryos with fewer muscle founder cells than wild-type, but more than seen in embryos with N^{int.SH.Scer\UAS} alone (driven by Scer\GAL4^da.G32 or Scer\GAL4^how-24B) alone. Also well differentiated muscles are found, in contrast to N^{int.SH.Scer\UAS} alone.

Co-expression of H^Scer\UAS.cGb and N^{int.SH.Scer\UAS} driven by Scer\GAL4^da.G32 results in the differentiation of CNS and some PNS structures, in contrast to N^{int.SH.Scer\UAS} alone (driven by Scer\GAL4^da.G32).

The addition of l(1)sc^Scer\UAS.cHa and da^Scer\UAS.cGa does not rescue the mesoderm phenotype of N^{int.SH.Scer\UAS} driven by Scer\GAL4^da.G32. Co-expression of H^Scer\UAS.cGb and N^{int.SH.Scer\UAS} driven by Scer\GAL4^da.G32 or Scer\GAL4^how-24B produces embryos with fewer muscle founder cells than wild-type, but more than seen in embryos with N^{int.SH.Scer\UAS} alone (driven by Scer\GAL4^da.G32 or Scer\GAL4^how-24B) alone. Also well differentiated muscles are found, in contrast to N^{int.SH.Scer\UAS} alone. Co-expression of H^Scer\UAS.cGb and N^{int.SH.Scer\UAS} driven by Scer\GAL4^da.G32 CNS and some PNS structures are seen to differentiate in contrast to N^{int.SH.Scer\UAS} alone (driven by Scer\GAL4^da.G32).

(Giebel, 1999)

Scer\GAL4^da.G32 mediated expression of N^{int.SH.Scer\UAS} blocks neural development completely, even when co-expressed with da^Scer\UAS.cGa and l(1)sc^Scer\UAS.cHa.

(Giebel et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

N^int.SH.UAS/Scer\GAL4^da.G32 partially rescues N^unspecified

(Zecchini et al., 1999)

Comments

Rescue of the neurogenic phenotype is good in both the dorsal and ventral regions of the embryo. The dorsal closure defect is not significantly rescued.

(Zecchini et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

L. Seugnet M. Haenlin

(Klein and Martinez Arias, 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

N^icd

(Tapanes-Castillo and Baylies, 2004)

N^{int.SH.Scer\UAS}

N^int.SH.UAS

N^intra

(Tapanes-Castillo and Baylies, 2004)

NICD

(Bivik et al., 2016)

Notch^intra

(Das and Knust, 2018)

Name Synonyms

Nintra

(Zecchini et al., 1999, Klein and Martinez Arias, 1998)

Secondary FlyBase IDs

References (27)

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