FlyBase Allele Report: Dmel\sv[UAS.cKa]

General Information

Symbol

Dmel\sv^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kavaler

FlyBase ID

FBal0096662

Feature type

allele

Associated gene

Dmel\sv

Associated Insertion(s)

Carried in Construct

P{UAS-sv.K}

Also Known As

UAS-PAX2, UAS-D-Pax2, UAS-sv, UAS-DPax2

Key Links

Transgenic product class

cDNA

(Kavaler et al., 1999)

wild_type

(Kavaler et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kavaler et al., 1999)

wild_type

(Kavaler et al., 1999)

Mutagen

in vitro construct

(Kavaler et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-sv.K}

Cytology

Description

UASt regulatory sequences drive expression of a 2.8kb EcoRI-XbaI fragment of the sv cDNA clone cpx1, which contains the entire protein coding region and 0.25kb of 3' untranslated sequences.

(Kavaler et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult head, with Scer\GAL4^ey.PH

(Jiao et al., 2001)

antenna, with Scer\GAL4^ey.PH

(Jiao et al., 2001)

chaeta, with Scer\GAL4^{neur-GAL4-A101}

(Andrews et al., 2009)

cone cell | increased number, with Scer\GAL4^sev.EP

(Charlton-Perkins et al., 2011)

external sensory organ | adult stage | somatic clone, with Scer\GAL4^{neur-GAL4-A101}

(Moore et al., 2004)

eye, with Scer\GAL4^ey.PH

(Jiao et al., 2001)

head bristle & tormogen cell, with Scer\GAL4^Bx-MS1096

(Kavaler et al., 1999)

head bristle & tormogen cell | conditional ts, with Scer\GAL4^hs.PB

(Kavaler et al., 1999)

head bristle & trichogen cell, with Scer\GAL4^Bx-MS1096

(Kavaler et al., 1999)

head bristle & trichogen cell | conditional ts, with Scer\GAL4^hs.PB

(Kavaler et al., 1999)

macrochaeta, with Scer\GAL4^Bx-MS1096

(Kavaler et al., 1999)

orbital bristle & trichogen cell | conditional ts, with Scer\GAL4^hs.PB

(Kavaler et al., 1999)

rhabdomere R7, with Scer\GAL4^sev.EP

(Charlton-Perkins et al., 2011)

scutum & macrochaeta & tormogen cell | conditional ts, with Scer\GAL4^hs.PB

(Kavaler et al., 1999)

scutum & macrochaeta & trichogen cell | conditional ts, with Scer\GAL4^hs.PB

(Kavaler et al., 1999)

scutum & microchaeta & tormogen cell, with Scer\GAL4^Bx-MS1096

(Kavaler et al., 1999)

scutum & microchaeta & trichogen cell, with Scer\GAL4^Bx-MS1096

(Kavaler et al., 1999)

scutum & microchaeta & trichogen cell | conditional ts, with Scer\GAL4^hs.PB

(Kavaler et al., 1999)

tormogen cell | adult stage | somatic clone, with Scer\GAL4^{neur-GAL4-A101}

(Moore et al., 2004)

trichogen cell | adult stage | ectopic | somatic clone, with Scer\GAL4^{neur-GAL4-A101}

(Moore et al., 2004)

wing, with Scer\GAL4^Bx-MS1096

(Kavaler et al., 1999)

Detailed Description

Statement

Reference

Ectopic expression of sv^Scer\UAS.cKa under the control of Scer\GAL4^sev.EP causes 26% of ommatidia to form one additional cone cell, an increase that is concomitant with a reduction in R7 photoreceptors. Compared to wild-type eyes, there is no significant change in primary pigment cell number in eyes expressing sv^Scer\UAS.cKa via Scer\GAL4^sev.EP.

(Charlton-Perkins et al., 2011)

Expression of sv^Scer\UAS.cKa under the control of Scer\GAL4^{neur-GAL4-A101} results in external sensory organs with two (9%) and three (1%) hair cells as well as the normal one hair (90%).

(Andrews et al., 2009)

Expression of sv^Scer\UAS.cKa under the control of Scer\GAL4^{neur-GAL4-A101} in clones in the notum results in external sensory organs with multiple hair shafts, some with misshapen external cells and in some cases loss of external cells.

(Moore et al., 2004)

Pharate adults expressing sv^Scer\UAS.cKa under the control of Scer\GAL4^ey.PH not only lack eyes, but often have no head except for the proboscis, while the thorax and abdomen are wild type. The headless phenotype is fully penetrant but shows variable expressivity. Four classes of phenotype are seen; class I pharate adults lack all head structures derived from the eye-antennal discs, class II consists of eyeless flies which lack most head structures and both antennae, class III consists of eyeless animals with large parts of the head missing but one or both antennae present and class IV consists of animals in which most of the head and one or both eyes of reduced size are present.

(Jiao et al., 2001)

Flies carrying sv^Scer\UAS.cKa and Scer\GAL4^hs.PB are normal when raised at 25^oC. A 15 minute heat shock at 37^oC during the pupal stage results in lethality, usually well before the pharate adult stage. A small fraction of the pupae develop to the pharate adult stage, and they all have some bristles on the notum and head that have extra shaft structures and lack sockets. Most commonly two smaller shafts are seen flanking a more normal-sized central shaft. Approximately 1/3 of the notal microchaetae are affected, and the orbital bristles on the head consistently show this phenotype. Flies expressing sv^Scer\UAS.cKa under the control of Scer\GAL4^Bx-MS1096 die as pharate adults with grossly malformed wings and bristle abnormalities. Bristles with extra shaft structures and lacking sockets are seen in particular regions of the notum and head, commonly having two smaller shafts flanking a more normal-sized central shaft. The microchaetae in the rows nearest the dorsocentral bristles consistently show this phenotype.

(Kavaler et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, sv^UAS.cKa has visible phenotype, suppressible | partially by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Jiao et al., 2001)

Scer\GAL4^ey.PH, sv^UAS.cKa has visible phenotype, suppressible by Myc^UAS.cZa, Scer\GAL4^ey.PH

(Jiao et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^sev.EP, sv^UAS.cKa has cone cell | increased number phenotype, enhanceable by Scer\GAL4^sev.EP/pros^RNAi.UAS

(Charlton-Perkins et al., 2011)

Scer\GAL4^sev.EP, sv^UAS.cKa has rhabdomere R7 phenotype, enhanceable by Scer\GAL4^sev.EP/pros^RNAi.UAS

(Charlton-Perkins et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, sv^UAS.cKa has adult head phenotype, suppressible by Myc^UAS.cZa, Scer\GAL4^ey.PH

(Jiao et al., 2001)

Scer\GAL4^ey.PH, sv^UAS.cKa has adult head phenotype, suppressible | partially by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Jiao et al., 2001)

Suppressor of

Statement

Reference

Scer\GAL4^{neur-GAL4-A101}/sv^UAS.cKa is a suppressor of chaeta | somatic clone phenotype of seq^U5

(Andrews et al., 2009)

Scer\GAL4^{neur-GAL4-A101}/sv^UAS.cKa is a suppressor of tormogen cell | somatic clone phenotype of seq^U5

(Andrews et al., 2009)

Other

Statement

Reference

Scer\GAL4^sev.EP, pros^L.UAS, sv^UAS.cKa has cone cell | increased number phenotype

(Charlton-Perkins et al., 2011)

Scer\GAL4^sev.EP, pros^RNAi.UAS, sv^UAS.cKa has eye phenotype

(Charlton-Perkins et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of both pros^L.Scer\UAS and sv^Scer\UAS.cKa consistently converts one extra cell into a cone cell. In contrast, no change in photoreceptor cell or primary pigment cell number is observed.

Co-expression of sv^Scer\UAS.cKa and pros^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sev.EP significantly increases the transformation of R7 photoreceptors into cone cells, with the majority of ommatidia forming five cone cells and lacking R7s. Mild eye roughening is also observed.

(Charlton-Perkins et al., 2011)

Expression of sv^Scer\UAS.cKa under the control of Scer\GAL4^{neur-GAL4-A101} in homozygous seq^U5 clones results in external sensory organs with 1 hair (89%) or 2 hairs (11%).

(Andrews et al., 2009)

Expression of both sv^Scer\UAS.cKa and ham^Scer\UAS.cMa under the control of Scer\GAL4^{neur-GAL4-A101} in clones in the notum results in the loss of external cells in almost 100% of external sensory organs in the clone.

(Moore et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58452

w^*; P{neoFRT}42D; P{UAS-sv.K}1/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

sv^Scer\UAS.cKa

sv^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kavaler

(Kavaler et al., 1999)

Secondary FlyBase IDs

References (10)

Report Sections

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