FlyBase Allele Report: Dmel\p38b[UAS.cAa]

General Information

Symbol

Dmel\p38b^UAS.cAa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Adachi-Yamada

FlyBase ID

FBal0096730

Feature type

allele

Associated gene

Dmel\p38b

Associated Insertion(s)

Carried in Construct

P{UAS-p38b.A}

Also Known As

UAS-p38b, UAS-p38b⁺

Key Links

Transgenic product class

wild_type

(Adachi-Yamada et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Adachi-Yamada et al., 1999)

Mutagen

in vitro construct

(Adachi-Yamada et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-p38b.A}

Cytology

Description

p38b is expressed under the control of UAS regulatory sequences.

(Adachi-Yamada et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

p38b

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm, with Scer\GAL4^cry.PZ

(Vrailas-Mortimer et al., 2014)

abnormal circadian rhythm, with Scer\GAL4^P0.5.Pdf

(Vrailas-Mortimer et al., 2014)

abnormal heat stress response, with Scer\GAL4^Mef2.PR

(Vrailas-Mortimer et al., 2011)

abnormal immune response | adult stage, with Scer\GAL4^da.G32

(Shinzawa et al., 2009)

abnormal immune response | adult stage, with Scer\GAL4^Pxn.PS

(Shinzawa et al., 2009)

abnormal neuroanatomy, with Scer\GAL4^cry.PZ

(Vrailas-Mortimer et al., 2014)

abnormal neuroanatomy, with Scer\GAL4^P0.5.Pdf

(Vrailas-Mortimer et al., 2014)

abnormal oxidative stress response, with Scer\GAL4^Mef2.PR

(Vrailas-Mortimer et al., 2011)

abnormal oxidative stress response, with Scer\GAL4^p38b-83

(Vrailas-Mortimer et al., 2011)

abnormal starvation stress response, with Scer\GAL4^Mef2.PR

(Vrailas-Mortimer et al., 2011)

long lived, with Scer\GAL4^DJ757

(Vrailas-Mortimer et al., 2011)

long lived, with Scer\GAL4^EDTP-DJ694

(Vrailas-Mortimer et al., 2011)

long lived, with Scer\GAL4^how-24B

(Vrailas-Mortimer et al., 2011)

long lived, with Scer\GAL4^Mef2.PR

(Vrailas-Mortimer et al., 2011)

long lived, with Scer\GAL4^Mhc.PW

(Vrailas-Mortimer et al., 2011)

long lived, with Scer\GAL4^Mhc.PW, Scer\GAL80^ts.αTub84B

(Vrailas-Mortimer et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

Overexpression of p38b^Scer\UAS.cAa in clock neurons using either Scer\GAL4^P0.5.Pdf or Scer\GAL4^cry.PZ results in an increased tau as well as an increase in the number of arrhythmic animals.

(Vrailas-Mortimer et al., 2014)

Flies expressing p38b^Scer\UAS.cAa in muscles throughout their lifetimes under the control of either Scer\GAL4^Mhc.PW, Scer\GAL4^Mef2.PR, Scer\GAL4^how-24B, Scer\GAL4^EDTP-DJ694 or Scer\GAL4^DJ757 live significantly longer than controls. Pan-neuronal expression under the control of Scer\GAL4^elav-C155 has no effect on lifespan.

Expression of p38b^Scer\UAS.cAa in muscles under the control of Scer\GAL4^Mhc.PW, and restricted to post-eclosion stages using Scer\GAL80^ts.αTub84B, results in a modest increase in lifespan compared to controls. Restricting p38b^Scer\UAS.cAa expression to adult stage neurons under the control of Scer\GAL4^elav-C155 has no effect on lifespan.

Expression of p38b^Scer\UAS.cAa in muscles under the control of Scer\GAL4^Mhc.PW suppresses the lethality that is usually seen in response to heat and dry starvation. No suppression of the phenotype occurs when p38b^Scer\UAS.cAa is expressed in neurons under the control of Scer\GAL4^elav-C155.

Expression of p38b^Scer\UAS.cAa in muscles under the control of Scer\GAL4^Mef2.PR confers additional resistance to hydrogen peroxide-induced oxidative stress compared to wild type. A similar phenotype is seen when p38b^Scer\UAS.cAa is expressed in the p38Kb expression domain under the control of Scer\GAL4^p38b-83.

(Vrailas-Mortimer et al., 2011)

Expression of p38b^Scer\UAS.cAa using Scer\GAL4^da.G32 or Scer\GAL4^Pxn.PS suppresses Salmonella-induced lethality without a subsequent reduction in bacterial load.

The tolerance of p38b^Scer\UAS.cAa Scer\GAL4^da.G32 flies to Salmonella infection is abolished if the engulfing function of hemocytes is blocked through the introduction of non-digestible latex beads.

Following infection with Salmonella, Scer\GAL4^Pxn.PS, p38b^Scer\UAS.cAa flies show an increase in intracellular persistence of Salmonella. This contrasts with controls, in which the intracellular Salmonella count clearly decreases during infection.

(Shinzawa et al., 2009)

Suppression by Df(2L)b82a2 or p38b^DN.Scer\UAS of the wing phenotype caused by tkv^CA.Scer\UAS expressed under the control of Scer\GAL4^71B is reduced if the flies are also coexpressing p38b^Scer\UAS.cAa.

(Adachi-Yamada et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^cry.PZ, p38b^UAS.cAa has abnormal circadian rhythm phenotype, suppressible by per⁰¹/per[+]

(Vrailas-Mortimer et al., 2014)

Scer\GAL4^cry.PZ, p38b^UAS.cAa has abnormal neuroanatomy phenotype, suppressible by Mef2^EnR.UAS, Scer\GAL4^cry.PZ

(Vrailas-Mortimer et al., 2014)

Scer\GAL4^Mef2.PR, p38b^UAS.cAa has long lived phenotype, suppressible by Sod2^RNAi.UAS.cKa/Scer\GAL4^Mef2.PR

(Vrailas-Mortimer et al., 2011)

Suppressor of

Statement

Reference

p38b^UAS.cAa, Scer\GAL4^cry.PZ is a suppressor of abnormal circadian rhythm phenotype of Mef2^EnR.UAS, Scer\GAL4^cry.PZ

(Vrailas-Mortimer et al., 2014)

p38b^UAS.cAa/Scer\GAL4^Mef2.PR is a suppressor of short lived phenotype of p38a¹, p38b^Δ25

(Vrailas-Mortimer et al., 2011)

p38b^UAS.cAa/Scer\GAL4^Mef2.PR is a suppressor of abnormal oxidative stress response phenotype of p38a¹, p38b^Δ25

(Vrailas-Mortimer et al., 2011)

p38b^UAS.cAa/Scer\GAL4^Mef2.PR is a suppressor of partially lethal - majority die phenotype of p38a¹, p38b^Δ25

(Vrailas-Mortimer et al., 2011)

p38b^UAS.cAa/Scer\GAL4^Mef2.PR is a suppressor of abnormal locomotor behavior | adult stage phenotype of p38a¹, p38b^Δ25

(Vrailas-Mortimer et al., 2011)

p38b^UAS.cAa, Scer\GAL4^sca-537.4 is a suppressor of visible phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/p38b^UAS.cAa is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of p38a¹, p38b^Δ25

(Vrailas-Mortimer et al., 2011)

Scer\GAL4^elav-C155/p38b^UAS.cAa is a non-suppressor of short lived phenotype of p38a¹, p38b^Δ25

(Vrailas-Mortimer et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

p38b^UAS.cAa, Scer\GAL4^sca-537.4 is a suppressor of microchaeta phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

p38b^UAS.cAa, Scer\GAL4^sca-537.4 is a suppressor of macrochaeta phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

A per⁰¹ heterozygous background rescues the circardian arrhythmia phenotype found upon expression of p38b^Scer\UAS.cAa under the control of Scer\GAL4^cry.PZ.

Overexpression of p38b^Scer\UAS.cAa background, rescues the arrhythmia phenotype in a Mef2^{Scer\UAS.T:Rep-en} expressing flies, from 84% of flies to 53% displaying arrhythmia.

Expression of p38b^Scer\UAS.cAa under the control of Scer\GAL4^cry.PZ results in an increased level of tau in cry-expressing neurons, which is further enhanced by inhibition of Mef2 through co-expression of Mef2^{Scer\UAS.T:Rep-en}.

(Vrailas-Mortimer et al., 2014)

Expression of p38b^Scer\UAS.cAa in muscles under the control of Scer\GAL4^Mef2.PR significantly rescues the reduction in lifespan seen in p38b^Δ25 Mpk2¹ double mutants. No rescue is seen when p38b^Scer\UAS.cAa is expressed in neurons under the control of Scer\GAL4^elav-C155.

Expression of p38b^Scer\UAS.cAa in muscles under the control of Scer\GAL4^Mef2.PR suppresses the geotaxis and walking defects seen in p38b^Δ25 Mpk2¹ double mutant females. No suppression is seen when p38b^Scer\UAS.cAa is expressed pan-neuronally under the control of Scer\GAL4^elav-C155.

Expression of p38b^Scer\UAS.cAa in muscles under the control of Scer\GAL4^Mef2.PR significantly rescues the increased sensitivity to hydrogen peroxide-induced oxidative stress seen in p38b^Δ25 Mpk2¹ double mutants.

Expression of p38b^Scer\UAS.cAa in muscles under the control of Scer\GAL4^Mef2.PR completely rescues the reduced viability seen in p38b^Δ25 Mpk2¹ double mutants.

Expression of Sod2^{dsRNA.Scer\UAS.cKa} suppresses the lifespan extension phenotype seen when p38b^Scer\UAS.cAa is expressed in muscles under the control of Scer\GAL4^Mef2.PR.

(Vrailas-Mortimer et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

p38b^UAS.cAa/Scer\GAL4^da.G32 rescues p38b^ex9

(Shinzawa et al., 2009)

Comments

Expression of p38b^Scer\UAS.cAa under the control of Scer\GAL4^da.G32 rescues the survival rate of Mpk2¹³ p38b^156A adults infected with either Enterobacter cloacae, Listeria monocytogenes or Beauveria bassiana to a level close to wild type.

(Chen et al., 2010)

Scer\GAL4^da.G32-mediated expression of p38b^Scer\UAS.cAa rescues the Salmonella-induced mortality of p38b^ex9 flies.

(Shinzawa et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

p38b^Scer\UAS.cAa

p38b^UAS.cAa

p38b^WT

(Balakireva et al., 2006)

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Adachi-Yamada

(Adachi-Yamada et al., 1999)

Secondary FlyBase IDs

References (12)

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