FlyBase Allele Report: Dmel\shrb[4]

General Information

Symbol

Dmel\shrb⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0102405

Feature type

allele

Associated gene

Dmel\shrb

Associated Insertion(s)

Carried in Construct

Also Known As

shrub^4-1, shrub⁴, shrb^4-1

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Gao et al., 1999)

Progenitor genotype

Cytology

Description

A 31bp deletion in the 2nd exon causes the shrb⁴ phenotype.

(Sweeney et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates fragile X syndrome

modeled by Fmr1^Δ50M

(Vita and Broadie, 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone | third instar larval stage

(Bäumers et al., 2019)

abnormal neuroanatomy | cell autonomous | pupal stage | somatic clone

(Loncle et al., 2015)

abnormal neuroanatomy | embryonic stage

(Sweeney et al., 2006)

lethal - all die during embryonic stage

(Sweeney et al., 2006)

some die during embryonic stage

(Sweeney et al., 2006)

Phenotype Manifest In

dendrite | cell autonomous | pupal stage | somatic clone

(Loncle et al., 2015)

dendrite | dorsal | embryonic stage

(Gao et al., 1999)

dendritic arborizing neuron | embryonic stage

(Sweeney et al., 2006)

dendritic arborizing neuron | embryonic stage (with Df(2R)Np5)

(Sweeney et al., 2006)

dendritic arborizing neuron | embryonic stage (with In(2LR)w45-32n)

(Sweeney et al., 2006)

embryonic/larval tracheocyte | embryonic stage

(Drees et al., 2023)

endosome | embryonic stage

(Drees et al., 2023)

larval dorsal multidendritic neuron | embryonic stage 16

(Sweeney et al., 2006)

larval dorsal multidendritic neuron | embryonic stage 16 (with Df(2R)Np5)

(Sweeney et al., 2006)

larval dorsal multidendritic neuron | embryonic stage 16 (with In(2LR)w45-32n)

(Sweeney et al., 2006)

larval dorsal multidendritic neuron | embryonic stage 17

(Sweeney et al., 2006)

larval dorsal multidendritic neuron | embryonic stage 17 (with Df(2R)Np5)

(Sweeney et al., 2006)

larval dorsal multidendritic neuron | embryonic stage 17 (with In(2LR)w45-32n)

(Sweeney et al., 2006)

larval dorsal multidendritic neuron ddaA | somatic clone

(Sweeney et al., 2006)

larval dorsal multidendritic neuron ddaB | somatic clone

(Sweeney et al., 2006)

larval dorsal multidendritic neuron ddaC | cell autonomous | pupal stage | somatic clone

(Loncle et al., 2015)

larval dorsal multidendritic neuron ddaC | somatic clone

(Sweeney et al., 2006)

larval dorsal multidendritic neuron ddaD | somatic clone

(Sweeney et al., 2006)

larval dorsal multidendritic neuron ddaE | somatic clone

(Sweeney et al., 2006)

larval dorsal multidendritic neuron ddaF | somatic clone

(Sweeney et al., 2006)

wing disc | somatic clone

(Bäumers et al., 2019)

Detailed Description

Statement

Reference

shrb⁴ homozygosity is cell lethal in the wing disc, as no clones are observed.

(Bäumers et al., 2019)

shrb⁴/shrb⁴ MARCM ddaC mutant clones show dendrite pruning defects at 18hr APF, compared to controls.

(Loncle et al., 2015)

shrb⁴ mutants do not exhibit an eye phenotype.

(Ahmad et al., 2009)

shrb⁴ mutants exhibit increased dendritic branching. Mutant ddaA neurons exhibit spine-like protrusions that are much longer than on wild-type ddaA neurons.

shrb⁴ zygotic mutant embryos do not exhibit detectable axon guidance or fasciculation defects in central nervous system and sensory neurons. However, some DA (dendritic arborisation) neurons exhibit abnormal axonal branching.

(Sweeney et al., 2006)

shrb⁴ mutant embryos die before reaching the larval stage. At 15-16 hours after egg-laying, dorsal dendrites in shrb⁴ mutants exhibit multiple dorsally oriented branches, compared to wild-type dorsal dendrites, which exhibit two dorsally oriented dendrites. At 17-18 and 19-20 hours after egg-laying, dendritic arborization (DA) neurons exhibit greatly reduced dendritic fields, with lateral branches often failing to reach the adjacent segment boundaries. The average dendritic field of dorsal clusters is reduced in shrb⁴ mutant embryos. Dendritic growth is similarly affected in embryos with the shrb⁴ mutation in trans with Df(2R)Np5 or In(2LR)w45-32n.

The shape of the dendritic trees of shrb⁴ mutant somatic clone ddaE neurons is similar to that in wild-type, but the number of dendritic termini is increased significantly because of an increase in fine, higher-order branches. shrb⁴ mutant clones of ddaF and ddaB neurons exhibit similar results. shrb⁴ ddaC somatic clone neurons exhibit a nearly 100% increase in dendritic termini. They also exhibit a high degree of branching complexity near the cell body. Dendritic fields of shrb⁴ ddaC somatic clones are also reduced in size. shrb⁴ mutant ddaA or ddaD neurons extend longer spine-like protrusions, in comparison to wild-type.

shrb⁴ mutant PNS neuron clones exhibit ectopic axonal branches in 21% in cases.

(Sweeney et al., 2006)

Exhibits reduced lateral branches in the dorsal cluster of embryonic dendrites. The dorsal dendrites extend only a fraction of the normal length and produce a few weakly elaborating lateral branches. The dendritic field in stage 17 embryos is drastically reduced. This is seen in over 95% of embryos. Mutants show no gross defects in muscle, anterior-posterior or dorsal-ventral patterning. The number of peripheral nervous system (PNS) neurons is the same as in wild-type. es and ch neurons retain their single dendrite morphology. There is no detectable abnormality in PNS and central nervous system axons.

(Gao et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

shrb⁴ has lethal | embryonic stage phenotype, suppressible | partially by Scer\GAL4^109(2)80/Mmus\Chmp4b^UAS.cSa

(Sweeney et al., 2006)

shrb⁴ has lethal | embryonic stage phenotype, suppressible | partially by Scer\GAL4^109(2)80/Mmus\Chmp4c^UAS.cSa

(Sweeney et al., 2006)

Enhancer of

Statement

Reference

shrb[+]/shrb⁴, Scer\GAL4^GMR.PF is an enhancer of increased cell death phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Suppressor of

Statement

Reference

shrb[+]/shrb⁴ is a suppressor of lethal - all die before end of larval stage | cold sensitive phenotype of Vps60^9.2

(Bäumers et al., 2019)

shrb[+]/shrb⁴ is a suppressor | partially of increased mortality during development phenotype of Ist1[+]/Ist1⁵¹, Vps60^9.2

(Bäumers et al., 2019)

Other

Statement

Reference

Ist1[+]/Ist1⁵¹, Vps60^9.2, shrb[+]/shrb⁴ has lethal - all die before end of larval stage phenotype

(Bäumers et al., 2019)

Ist1[+]/Ist1⁵¹, Vps60^9.2, shrb[+]/shrb⁴ has lethal - all die before end of pupal stage | heat sensitive phenotype

(Bäumers et al., 2019)

l(2)gd1^d7/l(2)gd1^SH0495, shrb[+]/shrb⁴ has lethal | third instar larval stage phenotype

(Troost et al., 2012)

Scer\GAL4^cv-c-C5, l(2)gd1^UAS.cJa, shrb[+]/shrb⁴ has visible phenotype

(Troost et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

shrb[+]/shrb⁴, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Other

Statement

Reference

Scer\GAL4^cv-c-C5, l(2)gd1^UAS.cJa, shrb[+]/shrb⁴ has wing vein phenotype

(Troost et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Rab7^GD11800 does not suppress the ectopic N activation phenotype in shrb⁴ follicle cell clones.

(Schneider et al., 2013)

The wing phenotype caused by expression of l(2)gd1^Scer\UAS.cJa under the control of Scer\GAL4^C5 is strongly modified if the flies are also heterozygous for shrb⁴.

(Troost et al., 2012)

Xenogenetic Interactions

Statement

Reference

A single copy of shrb⁴ significantly enhances the Hsap\CHMP2B^{Intron5.Scer\UAS} Scer\GAL4^GMR.PF phenotype in 1-day-old flies.

A shrb⁴ heterozygous background significantly enhances the Scer\GAL4^GMR.PF :Hsap\CHMP2B^{Intron5.Scer\UAS} phenotype.

(Ahmad et al., 2009)

Expression of Mmus\Chmp4b^Scer\UAS.cSa under the control of Scer\GAL4^109(2)80 partially rescues the lethality of shrb⁴ mutants (to approximately 70% survival rate).

Expression of Mmus\Chmp4c^Scer\UAS.cSa under the control of Scer\GAL4^109(2)80 partially rescues the lethality of shrb⁴ mutants (to approximately 40% survival rate).

(Sweeney et al., 2006)

Complementation and Rescue Data

Rescued by

shrb⁴ is rescued by shrb^UAS.cSa/Scer\GAL4^αTub84B.PL

(Sweeney et al., 2006)

shrb⁴ is rescued by Scer\GAL4^109(2)80/shrb^UAS.cSa

(Sweeney et al., 2006)

Partially rescued by

shrb⁴ is partially rescued by Scer\GAL4^hh-Gal4/shrb^UAS.Tag:MYC

(Bäumers et al., 2019)

Comments

Rescue of shrb⁴ embryonic lethality with shrb^Scer\UAS.cSa, under the control of Scer\GAL4^αTub84B.PL allows survival of 99% of mutant embryos to the third instar larval stage.

Expression of two copies of shrb^Scer\UAS.cSa in neurons of the peripheral nervous system, under the control of Scer\GAL4^109(2)80, rescues the average dendritic field size of dorsal cluster DA neurons in shrb⁴ mutants to near wild-type levels. The rescued dorsal clusters contain a number of lateral branches that are largely absent in shrb⁴ mutant clusters

(Sweeney et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Induced on: a chromosome containing Avic\GFP^{S65T.Scer\UAS} driven by Scer\GAL4^109(2)80.

(Gao et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

shrb^4-1

(McMillan et al., 2016, Drusenheimer et al., 2015, Ahmad et al., 2009)

shrb⁴

(Juhász et al., 2008, Sweeney et al., 2006)

shrub^4-1

(Baeumers et al., 2020, Bäumers et al., 2019, Schneider et al., 2013, Troost et al., 2012)

shrub⁴

(Drees et al., 2023, Vita and Broadie, 2017, Loncle et al., 2015, Dong et al., 2014)

Name Synonyms

shrub4

(Sweeney et al., 2006)

Secondary FlyBase IDs

References (15)

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