FlyBase Allele Report: Dmel\krz[S047819]

Embryos from krz^S047819 maternal germline clones show delayed ventral furrow formation and large ventral holes. The same is observed in embryos from krz^S047819 homozygous mothers rescued to adulthood by krz^VL-A.Tag:SBP.

(Chai et al., 2019)

krz^d1/krz^S047819 mutant animals survive until the third larval instar, where they become immobile and flaccid and form melanotic tumors. The imaginal discs of these mutant larvae are very reduced in size and display increased cell death.

Expression of krz^Scer\UAS.cMa in the central nervous system under the control of Scer\GAL4^wor.PA rescues the lethality of krz^d1/krz^S047819. The surviving flies are sterile and display slightly folded wings. Wing discs in rescued larvae have a normal appearance.

(Molnar et al., 2011)

49% of krz^S047819 mutant cuticles display a large hole either at the anterior or posterior region of the embryo, 31% of mutant embryos display two holes, and 17% of the cuticles display twisting along the anterior-posterior axis. Mutants display gastrulation defects and embryonic lethality.

The embryonic nervous system is disrupted in stage 15 krz^S047819 embryos, and animals display holes in the nerve cord, or a twisted nerve cord phenotype.

(Tipping et al., 2010)

Homozygous adults which have been rescued from lethality by expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^hs.PB during development (using twice daily heat shocks) die at a faster rate than heterozygous siblings or wild-type controls on medium containing no added NaCl. On medium containing 0.4M NaCl, homozygous adults die at a much faster rate than heterozygous siblings or wild-type controls.

(Johnson et al., 2008)

krz^S047819 homozygous adults which have been rescued from lethality by expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^hs.PB show a reduced initial activity phase (during the first minute) in an open field arena compared to control flies. The spontaneous locomotor activity is not significantly different from control flies from the second minute onwards (the assay was carried out for 10 minutes).

krz^S047819 homozygous adults which have been rescued from lethality by expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^hs.PB and which are also expressing krz^Scer\UAS.cRa under the control of Scer\GAL4^{Or83b.T:Hsim\VP16} still show a reduced initial activity phase in an open field arena compared to control flies.

There are no significant differences in spontaneous activity between krz^S047819 homozygous adults which have been rescued from lethality by expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^hs.PB and control flies measured in the dark over a period of days.

krz^S047819 homozygous adults which have been rescued from lethality by expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^hs.PB fail to show a robust increase in activity after mechanical stimulation, in contrast to control flies.

(Liu et al., 2007)

The majority of homozygous krz^S047819 mutants die as embryos, and less than 25% reach the third instar larval stage

(Mukherjee et al., 2005)

Homozygotes die throughout development. Most animals fail to hatch and none of the surviving larvae live to puparium formation. Prior to death the mutant larvae become immobile and flaccid. Third instar larvae develop melanotic tumours, which form throughout the body cavity but most frequently collect in the posterior of the larva. Tumours are frequently found floating free in the haemocoel. Large numbers of lamellocytes are found circulating through the haemocoel of mutant third instar larvae (these cells typically form in wild-type pupae). The fat bodies of mutant larvae shortly after the second moult have a rough appearance compared to those of wild-type larvae. At approximately 18-20 hours after the second moult the fat bodies of surviving mutant larvae begin to dissociate into individual cells (the wild-type fat body does not normally dissociate until after pupariation). Melanotic tumours begin to form on the surface of the mutant fat bodies shortly after the second moult.

(Roman et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

krz^S047819 has lethal phenotype, suppressible by Scer\GAL4^da.PU/Hsap\ARRB1^{UAS.cAa.Tag:HA}

(Chai et al., 2019)

krz^S047819 has lethal phenotype, suppressible by Scer\GAL4^da.PU/Hsap\ARRB2^{UAS.cAa.Tag:HA}

(Chai et al., 2019)

Scer\GAL4^da.G32, krz^S047819/krz[+], rl^Sem.S.UAS has visible phenotype, suppressible by krz^{UAS.Tag:GS-TAP}/Scer\GAL4^da.G32

(Tipping et al., 2010)

Scer\GAL4^da.G32, krz^S047819/krz[+], rl^Sem.S.UAS has partially lethal - majority die phenotype, suppressible by krz^{UAS.Tag:GS-TAP}/Scer\GAL4^da.G32

(Tipping et al., 2010)

Enhancer of

Statement

Reference

krz^S047819/krz[+] is an enhancer of visible phenotype of Scer\GAL4^da.G32, rl^Sem.S.UAS

(Tipping et al., 2010)

krz^S047819/krz[+] is an enhancer of partially lethal - majority die phenotype of Scer\GAL4^da.G32, rl^Sem.S.UAS

(Tipping et al., 2010)

krz^S047819/krz[+] is an enhancer of visible | recessive phenotype of dx^S1M

(Mukherjee et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^da.G32, krz^S047819/krz[+], rl^Sem.S.UAS has wing vein phenotype, suppressible by krz^{UAS.Tag:GS-TAP}/Scer\GAL4^da.G32

(Tipping et al., 2010)

Scer\GAL4^da.G32, krz^S047819/krz[+], rl^Sem.S.UAS has eye phenotype, suppressible by krz^{UAS.Tag:GS-TAP}/Scer\GAL4^da.G32

(Tipping et al., 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^da.G32, krz^S047819/krz[+], rl^Sem.S.UAS has wing phenotype, non-suppressible by krz^{UAS.Tag:GS-TAP}/Scer\GAL4^da.G32

(Tipping et al., 2010)

Enhancer of

Statement

Reference

krz^S047819/krz[+] is an enhancer of wing phenotype of Scer\GAL4^da.G32, rl^Sem.S.UAS

(Tipping et al., 2010)

krz^S047819/krz[+] is an enhancer of wing vein phenotype of Scer\GAL4^da.G32, rl^Sem.S.UAS

(Tipping et al., 2010)

krz^S047819/krz[+] is an enhancer of eye phenotype of Scer\GAL4^da.G32, rl^Sem.S.UAS

(Tipping et al., 2010)

krz^S047819/krz[+] is an enhancer of wing margin phenotype of dx^S1M

(Mukherjee et al., 2005)

krz^S047819/krz[+] is an enhancer of wing vein phenotype of dx^S1M

(Mukherjee et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^da.G32 in a krz^S047819/+ mutant background results in the development of even more additional veins in the wing and a further reduction in wing size.

Expression of rl^{Sem.S.Scer\UAS} and krz^{Scer\UAS.T:Zzzz\GS-TAP} under the control of Scer\GAL4^da.G32 in a krz^S047819/+ mutant background restores the wing vein phenotype to that seen in animals expressing just rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^da.G32 in a wild-type background. Wing size is larger in these flies compared to animals expressing rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^da.G32 in a wild-type background.

The mild irregularities in ommatidial pattern observed in animals expressing rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^da.G32 is strongly enhanced in a krz^S047819/+ mutant background, and smaller rougher eyes are observed.

Expression of krz^{Scer\UAS.T:Zzzz\GS-TAP} restores the regular ommatidial pattern and size of the eye in animals expressing rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^da.G32 in a krz^S047819/+ mutant background.

The survival rate of animals expressing rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^da.G32 drops to 8% in a krz^S047819/+ mutant background.

The survival rate of animals expressing rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^da.G32 in a krz^S047819/+ mutant background increases to 38% when krz^{Scer\UAS.T:Zzzz\GS-TAP} is co-expressed.

(Tipping et al., 2010)

The notching and vein thickening phenotypes seen in dx^S1M hemizygous males are enhanced by krz^S047819/+. Survival of N^Ax-9/N^Ax-E2 dx^ENU/+ flies to adulthood is significantly decreased by krz^S047819/+.

(Mukherjee et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

krz⁴

(Roman et al., 2000)

Fails to complement

krz³

(Roman et al., 2000)

krz^L8

(Roman et al., 2000)

krz^S095214

(Roman et al., 2000)

Rescued by

krz^S047819 is rescued by krz^{ΔLIQLD.Tag:SBP}

(Chai et al., 2019)

krz^S047819 is rescued by krz^F-A.Tag:SBP

(Chai et al., 2019)

krz^S047819 is rescued by krz^R-A.Tag:SBP

(Chai et al., 2019)

krz^S047819 is rescued by krz^R-E.Tag:SBP

(Chai et al., 2019)

krz^S047819 is rescued by krz^Tag:SBP

(Chai et al., 2019)

krz^S047819 is rescued by krz^VL-A.Tag:SBP

(Chai et al., 2019)

krz^S047819 is rescued by krz^+t5.7

(Tipping et al., 2010)

krz^S047819 is rescued by krz^UAS.Tag:TAP/Scer\GAL4^da.G32

(Kyriakakis et al., 2008)

krz^S047819 is rescued by krz^{UAS.Tag:GS-TAP}/Scer\GAL4^da.G32

(Kyriakakis et al., 2008)

krz^S047819 is rescued by krz^+t5.8

(Roman et al., 2000)

krz^S047819 is rescued by krz^UAS.cRa/Scer\GAL4^hs.PB

(Roman et al., 2000)

krz^S047819 is rescued by krz^+t12

(Roman et al., 2000)

Partially rescued by

krz^S047819 is partially rescued by krz^KK-A.Tag:SBP

(Chai et al., 2019)

krz^S047819 is partially rescued by krz^{KKR-A.Tag:SBP}

(Chai et al., 2019)

krz^S047819 is partially rescued by krz^{RVL-A.Tag:SBP}

(Chai et al., 2019)

krz^S047819/krz^d1 is partially rescued by krz^UAS.cMa/Scer\GAL4^wor.PA

(Molnar et al., 2011)

krz^S047819 is partially rescued by Scer\GAL4^elav-C155/krz^UAS.cRa

(Roman et al., 2000)

Not rescued by

krz^S047819 is not rescued by krz^{KKVL-A.Tag:SBP}

(Chai et al., 2019)

krz^S047819 is not rescued by Scer\GAL4^Kr.PM/krz^UAS.cRa

(Roman et al., 2000)

Comments

(Molnar et al., 2011)

Expression of krz^+t5.7 in krz^S047819 mutant animals fully rescues the embryonic nervous system and cuticule phenotype.

(Tipping et al., 2010)

Expression of krz^{Scer\UAS.T:Zzzz\TAP} under the control of Scer\GAL4^da.G32 in a krz^S047819 mutant background completely rescues lethality.

Expression of krz^{Scer\UAS.T:Zzzz\GS-TAP} under the control of Scer\GAL4^da.G32 in a krz^S047819 mutant background completely rescues lethality.

(Kyriakakis et al., 2008)

Fully complements the male sterility of mod⁰⁷⁵⁷⁰. Lethality is rescued by krz^+t12. krz^+t5.8 rescues the lethality, formation of melanotic tumours and disaggregation of fat bodies seen in krz^S047819 animals. Expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^hs.PB using heat shock is sufficient to rescue some of the krz^S047819 mutant animals at each larval instar. Rescue of lethality becomes significantly more efficient at older stages. Expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^Kr.PM does not rescue the lethality of krz^S047819 animals. Expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^elav-C155 rescues the lethality of krz^S047819 animals. Approximately 1/3 of the rescued animals fail to expand their wings after eclosion. Melanotic tumours are visible in the abdomens of most of the rescued animals.

(Roman et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Complements: l(3)S041303^S041303. Complements: l(3)S024314b^S024314b. Complements: l(3)S099801b^S099801b. Complements: l(3)S134408b^S134408b. Complements: l(3)06886⁰⁶⁸⁸⁶. Complements: l(3)rH304^rH304. Complements: l(3)06497⁰⁶⁴⁹⁷. Complements: mod⁰⁷⁵⁷⁰. The P{lacW} element is responsible for the lethal phenotype.

(Roman et al., 2000)

ird6¹ fails to complement the lethality of krz^S047819.

(Wu et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

478/19

(Roman et al., 2000)

krz¹

(Chai et al., 2019, Couturier et al., 2014, Anjum et al., 2013, Molnar et al., 2011, Tipping et al., 2010, Johnson et al., 2008, Kyriakakis et al., 2008, Liu et al., 2007, Mukherjee et al., 2005, Wu et al., 2001, Roman et al., 2000)

krz^S047819

krz^s047819

(Tipping et al., 2010)

kurtz¹

(Roman et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (12)

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