FlyBase Allele Report: Dmel\Cbl[F165]

General Information

Symbol

Dmel\Cbl^F165

Species

D. melanogaster

Name

FlyBase ID

FBal0118589

Feature type

allele

Associated gene

Dmel\Cbl

Associated Insertion(s)

Carried in Construct

Also Known As

D-cbl^F165

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Pai et al., 2000)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q116term.

(Pai et al., 2000)

Nucleotide substitution: C?T.

(Pai et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:8,425,654..8,425,654 [+]

Nucleotide change:

C8425654T

Reported nucleotide change:

C?T

Amino acid change:

Q116term | Cbl-PA; Q116term | Cbl-PB

Reported amino acid change:

Q116term

(Pai et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal endomitotic cell cycle | adult stage | somatic clone

(Wang et al., 2015)

abnormal endomitotic cell cycle | third instar larval stage

(Wang et al., 2015)

lethal | pupal stage | recessive

(Pai et al., 2006)

lethal | recessive

(Jekely et al., 2005)

partially lethal - majority die | recessive | third instar larval stage

(Pai et al., 2006)

Phenotype Manifest In

border follicle cell | somatic clone

(Jekely et al., 2005)

cephalopharyngeal skeleton | germline clone | rescuable maternal effect

(Pai et al., 2000)

cephalopharyngeal skeleton | maternal effect | somatic clone

(Pai et al., 2000)

cytoophidium | adult stage | female | somatic clone

(Wang et al., 2015)

cytoophidium | somatic clone | third instar larval stage

(Wang et al., 2015)

dorsal appendage | maternal effect, with Cbl^L.Hsp83

(Pai et al., 2006)

dorsal appendage | maternal effect | somatic clone

(Pai et al., 2000)

egg chorion | maternal effect, with Cbl^L.Hsp83

(Pai et al., 2006)

egg chorion | maternal effect | somatic clone

(Pai et al., 2000)

embryo | maternal effect | somatic clone

(Pai et al., 2000)

follicle cell | adult stage | somatic clone

(Wang et al., 2015)

salivary gland | somatic clone | third instar larval stage

(Wang et al., 2015)

salivary gland | third instar larval stage

(Wang et al., 2015)

ventral denticle belt | maternal effect | somatic clone

(Pai et al., 2000)

wing | somatic clone

(Pai et al., 2000)

wing vein | ectopic, with Cbl^S.Hsp83

(Pai et al., 2006)

wing vein | ectopic | somatic clone

(Pai et al., 2000)

Detailed Description

Statement

Reference

Cbl^F165 heterozygote adult females do not display any defects in the number of cytoophidia (CTPsyn filaments) in the egg chambers (when kept at 29[o]C) or in DNA replication (measured by BrdU incorporation) in stage 9 egg chambers compared to respective controls.

Majority of follicle cells in Cbl^F165 homozygous somatic clones display significantly reduced number or complete absence of cytoophidia (CTPsyn filaments) in stage 9 and 10 egg chambers compared to controls. However, the cytoophidia are maintained as normal in Cbl^F165 mutant clones in younger egg chambers - before stage 6 and still in the mitotic stages of their development and the macro-cytoophidia in germline clone cells are also not affected. Decrease in cytoophidia numbers is also observed Cbl^F165/Cbl^F165 somatic clones in salivary glands of third instar larvae.

Follicle cells in Cbl^BB28 homozygous mutant somatic clones display endocycle defects in stage 9 and 10A (but not 10B) egg chambers: the number of cells in S-phase (BrdU positive) is significantly decreased compared to controls.

Cbl^F165 homozygous third instar larvae are of a normal size but their salivary glands are smaller and display endocycle defects (measured by BrdU incorporation) compared to wild-type. However, average nuclear size of cells in Cbl^F165 somatic clones (both in salivary glands in third instar larvae and follicle cells in adult females) is not significantly changed compared to twin clone controls.

(Wang et al., 2015)

Cbl^F165 heterozyous ovaries do not exhibit any abnormalities.

(Epstein et al., 2009)

Cbl^F165 mutant cells are preferentially located at the front of the cluster during border cell posterior and dorsal migration in mosaic border cell clusters consisting of both wild-type and mutant cells.

(Bianco et al., 2007)

Border follicle cells are correctly specified in somatic clones of Cbl^F165 homozygous cells but their migration is severely defective and sometimes fails completely. However, around 50% of migrating clusters do eventually reach the oocyte.

(Jekely et al., 2005)

Females containing somatic clones of Cbl^F165 induced in the follicle cells produce dorsalised embryos and egg shells; in some cases egg shells with two dorsal appendages positioned further apart than normal are produced (characteristic of a weakly dorsalised phenotype), and partially or completely dorsalised embryos are produced as evidenced by the loss of ventrally derived structures. Only clones at ventral positions within the follicle cell epithelium result in a dorsalised embryo phenotype, such as a missing head skeleton, reduction of ventral structures (such as denticle belts) in the thoracic and anterior abdominal segments and a twisted embryo. Clones that are confined to the dorsal half of the follicle epithelium do not produce a visible embryonic mutant phenotype. When dorsolateral follicle cells are homozygous for Cbl^F165, dorsal appendages are shifted to more lateral positions than normal. When more lateral cells (adjacent to the dorsal appendages) are homozygous for Cbl^F165, extra or wider dorsal appendages are produced. Somatic clones of Cbl^F165 induced in the wing result in an extra vein phenotype. Embryos lacking both maternal and zygotic Cbl function (derived from homozygous germline clones) have a distinct head defect. Zygotic Cbl function is able to rescue the maternal lack of Cbl function, resulting in normal embryos that hatch.

(Pai et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cbl^F165 has abnormal endomitotic cell cycle | somatic clone | adult stage phenotype, suppressible by Ctps^UAS.Tag:FLAG/Scer\GAL4^hs.PU

(Wang et al., 2015)

NOT suppressed by

Statement

Reference

Cbl^F165 has abnormal endomitotic cell cycle | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^hs.PU/Ctps^{C399G.UAS.Tag:FLAG}

(Wang et al., 2015)

Enhancer of

Statement

Reference

Cbl^F165 is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

Cbl^F165/Cbl[+] is an enhancer of abnormal endomitotic cell cycle | adult stage phenotype of Ctps^GD4740, Scer\GAL4^CY2

(Wang et al., 2015)

NOT Enhancer of

Statement

Reference

Cbl^F165 is a non-enhancer of visible phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Suppressor of

Statement

Reference

Cbl^F165 is a suppressor of increased cell death phenotype of Rbf^120a

(Truscott et al., 2014)

Cbl^F165/Cbl[+] is a suppressor | partially of abnormal endomitotic cell cycle phenotype of Fmr1^Δ50M/Fmr1³

(Epstein et al., 2009)

Cbl^F165/Cbl[+] is a suppressor of visible phenotype of Scer\GAL4^ap-md544, Socs36E^UASp.cCa

(Callus and Mathey-Prevot, 2002)

NOT Suppressor of

Statement

Reference

Cbl^F165 is a non-suppressor of visible phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cbl^F165 has border follicle cell | somatic clone phenotype, enhanceable by Egfr^UAS.cBa/Scer\GAL4^slbo.2.6

(Jekely et al., 2005)

Cbl^F165 has border follicle cell | somatic clone phenotype, enhanceable by Scer\GAL4^slbo.2.6/Pvr^UAS.cDa

(Jekely et al., 2005)

Cbl^F165 has border follicle cell | somatic clone phenotype, enhanceable by spri^6G1/spri^6G1

(Jekely et al., 2005)

Suppressed by

Statement

Reference

Cbl^F165 has follicle cell | somatic clone | adult stage phenotype, suppressible by Ctps^UAS.Tag:FLAG/Scer\GAL4^hs.PU

(Wang et al., 2015)

Cbl^F165 has cytoophidium | female | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^hs.PU/Ctps^{C399G.UAS.Tag:FLAG}

(Wang et al., 2015)

Cbl^F165 has follicle cell | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^hs.PU/Ctps^{C399G.UAS.Tag:FLAG}

(Wang et al., 2015)

Cbl^F165 has border follicle cell | somatic clone phenotype, suppressible by grk^unspecified/grk[+]

(Jekely et al., 2005)

NOT suppressed by

Statement

Reference

Cbl^F165 has cytoophidium | female | somatic clone | adult stage phenotype, non-suppressible by Ctps^UAS.Tag:FLAG/Scer\GAL4^hs.PU

(Wang et al., 2015)

Cbl^F165 has follicle cell | somatic clone | adult stage phenotype, non-suppressible by Ctps^UAS.Tag:FLAG/Scer\GAL4^hs.PU

(Wang et al., 2015)

Cbl^F165 has follicle cell | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^hs.PU/Ctps^{C399G.UAS.Tag:FLAG}

(Wang et al., 2015)

Enhancer of

Statement

Reference

Cbl^F165 is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

Cbl^F165/Cbl[+] is an enhancer of follicle cell | adult stage phenotype of Ctps^GD4740, Scer\GAL4^CY2

(Wang et al., 2015)

Cbl^F165/Cbl[+] is an enhancer of nucleus | adult stage phenotype of Ctps^GD4740, Scer\GAL4^CY2

(Wang et al., 2015)

Cbl^F165/Cbl[+] is an enhancer of interommatidial precursor cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}, cindr^RNAi.UAS

(Johnson et al., 2012)

Cbl^F165/Cbl[+] is an enhancer of primary pigment cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}, cindr^RNAi.UAS

(Johnson et al., 2012)

Cbl^F165/Cbl[+] is an enhancer of cone cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}, cindr^RNAi.UAS

(Johnson et al., 2012)

NOT Enhancer of

Statement

Reference

Cbl^F165 is a non-enhancer of eye phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Suppressor of

Statement

Reference

Cbl^F165 is a suppressor of eye disc phenotype of Rbf^120a

(Truscott et al., 2014)

Cbl^F165/Cbl[+] is a suppressor of nurse cell phenotype of Fmr1³

(Epstein et al., 2009)

Cbl^F165/Cbl[+] is a suppressor | partially of nurse cell phenotype of Fmr1^Δ50M/Fmr1³

(Epstein et al., 2009)

Cbl^F165/Cbl[+] is a suppressor | partially of nurse cell | increased number phenotype of Fmr1³

(Epstein et al., 2009)

Cbl^F165 is a suppressor of eye photoreceptor cell | somatic clone phenotype of mop^T612

(Miura et al., 2008)

Cbl^F165/Cbl[+] is a suppressor | partially of anterior crossvein phenotype of Scer\GAL4^en-e16E, Socs36E^UASp.cCa

(Callus and Mathey-Prevot, 2002)

Cbl^F165/Cbl[+] is a suppressor | partially of wing phenotype of Scer\GAL4^en-e16E, Socs36E^UASp.cCa

(Callus and Mathey-Prevot, 2002)

NOT Suppressor of

Statement

Reference

Cbl^F165/Cbl[+] is a non-suppressor of nurse cell | increased number phenotype of Fmr1^Δ50M/Fmr1³

(Epstein et al., 2009)

Cbl^F165 is a non-suppressor of eye phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Other

Statement

Reference

Cbl^F165, Ctps^GD4740, Scer\GAL4^CY2 has egg phenotype

(Wang et al., 2015)

Cbl^F165, Prosβ6¹ has egg chamber phenotype

(Wang et al., 2015)

Cbl^F165, Prosβ6¹ has cytoophidium | female | adult stage phenotype

(Wang et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The number of cytoophidia (CTPsyn filaments) in the egg chambers of adult Prosβ6¹,Cbl^F165 double heterozygote females is significantly reduced compared to either Prosβ6¹ or Cbl^F165 single heterozygotes.

About a fifth of eggs laid by Cbl^F165 heterozygous females expressing CTPsyn^GD4740 under the control of Scer\GAL4^CY2 has a defective collapsed phenotype that is not observed in eggs laid by Cbl^F165/+ flies.

The loss of cytoophidia (CTPsyn filaments) observed in somatic clones of Cbl^F165 homozygous mutant follicle cells is not suppressed by expression of CTPsyn^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^hs.PU in the clones, although the CTPsyn^{Scer\UAS.T:Zzzz\FLAG} protein can be incorporated in the cytoophidia filament structure together with endogenous CTPsyn protein in wild-type cells.

The loss of cytoophidia observed in somatic clones of Cbl^F165 homozygous mutant follicle cells is suppressed by expression of CTPsyn^{C399G.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^hs.PU in the clones, although the CTPsyn^{C399G.Scer\UAS.T:Zzzz\FLAG} protein cannot be incorporated in the cytoophidia filament structure together with endogenous CTPsyn protein in wild-type cells.

The endocycle defects (decrease in DNA replication measured by BrdU incorporation) seen in somatic clones of Cbl^F165 homozygous mutant follicle cells is partially suppressed by expression of CTPsyn^{Scer\UAS.T:Zzzz\FLAG} but not suppressed by expression of CTPsyn^{C399G.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^hs.PU in the clones.

The decrease in percentage of follicle cells undergoing DNA replication (measured by BrdU incorporation) observed in stage 9 egg chambers as well as the decreased follicle cell nuclear size in stage 10A egg chamber expressing CTPsyn^GD4740 under the control of Scer\GAL4^CY2 is further decreased by combination with Cbl^F165 in heterozygous state.

(Wang et al., 2015)

The generation of Cbl^F165 mutant eye disc clones suppresses the amount of cell death seen in Rbf^120a mutant eye discs.

(Truscott et al., 2014)

Cbl^F165/+ enhances the mis-patterning of interommatidial precursor cells, primary primary pigment cells and cone cells caused by co-expression of cindr^{dsRNA.Scer\UAS} and cindr^{dsRNA.PC.PD.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

(Johnson et al., 2012)

Cbl^F165 is a dominant suppressor of the germ cell proliferation defects in Fmr1³ and Fmr1³/Fmr1^Δ50M mutant ovaries.

The fewer germ cell phenotype in Fmr1³ mutant ovaries is completely suppressed by Cbl^F165/+, while the supernumerary germ cell phenotype is suppressed only partially.

The fewer germ cell phenotype in Fmr1³/Fmr1^Δ50M ovaries is largely suppressed by Cbl^F165/+, while the supernumerary germ cell phenotype is not suppressed at all.

(Epstein et al., 2009)

The loss of photoreceptor cells seen in homozygous mop^T612 clones in the eye disc is rescued if the clones are also homozygous for Cbl^F165.

(Miura et al., 2008)

Defective migration of border follicle cells in Cbl^F165 homozygous somatic clones is suppressed by grk^unspecified/+ but enhanced by Egfr^Scer\UAS.cBa or Pvr^Scer\UAS.cDa with Scer\GAL4^slbo.2.6.

spri^6G1/spri^6G1 also enhances the migration defects seen in border follicle cells in Cbl^F165 homozygous somatic clones. I these animals, border follicle cell clusters rarely reach the oocyte.

(Jekely et al., 2005)

Expression of Socs36E^{Scer\UAS.P\T.cCa} under the control of Scer\GAL4^en-e16E in a Cbl^F165/+ background results in a partial rescue of the Socs36E^{Scer\UAS.P\T.cCa} anterior crossvein phenotype, with a reduction in penetrance from 95 to 48%. The penetrance of the outstretched wing phenotype of Scer\GAL4^ap-md544>Socs36E^{Scer\UAS.P\T.cCa} flies is reduced from 82 to 54% when flies have a Cbl^F165/+ background.

(Callus and Mathey-Prevot, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Cbl^F165 is rescued by Cbl^L.Hsp83

(Wang and Pai, 2011)

Cbl^F165 is rescued by Cbl^S.Hsp83

(Wang and Pai, 2011)

Cbl^F165 is rescued by Cbl^L.mPR.Hsp83

(Wang and Pai, 2011)

Cbl^F165 is rescued by Cbl^S.Hsp83

(Pai et al., 2006)

Cbl^F165 is rescued by Cbl^{S.αTub84B.PJ}

(Jekely et al., 2005)

Cbl^F165 is rescued by Cbl^{L.αTub84B.PJ}

(Jekely et al., 2005)

Partially rescued by

Cbl^F165 is partially rescued by Cbl^S.Hsp83

(Wang et al., 2015)

Cbl^F165 is partially rescued by Cbl^L.mPR.Hsp83

(Wang et al., 2015)

Not rescued by

Cbl^F165 is not rescued by Cbl^{S.Δ70Z.Hsp83}

(Wang et al., 2015)

Cbl^F165 is not rescued by Cbl^{L.Δ70Z.Hsp83}

(Wang et al., 2015)

Cbl^F165 is not rescued by Cbl^{L.Δ70Z.Hsp83}

(Wang and Pai, 2011)

Cbl^F165 is not rescued by Cbl^{Ring.S.αTub84B}

(Jekely et al., 2005)

Comments

Expression of either Cbl^L.mPR.Hsp83 or Cbl^S.Hsp83 in the follicle cell somatic clones homozygous for Cbl^F165 partially rescues the loss of cytoophidia in stage 9 egg chambers - the proportion of cells completely lacking cytoophidia is significantly decreased.

Expression of either Cbl^{S.Δ70Z.Hsp83} or Cbl^{L.Δ70Z.Hsp83} in the follicle cell somatic clones homozygous for Cbl^F165 does not rescues the loss of cytoophidia (CTPsyn filaments) in stage 9 egg chambers.

(Wang et al., 2015)

Expression of Cbl^L.mPR.Hsp83 rescues the lethality of Cbl^F165, and 75-80% of animals develop to adults.

Expression of Cbl^{L.Δ70Z.Hsp83} fails to rescue Cbl^F165.

(Wang and Pai, 2011)

Lethality due to Cbl^F165/Cbl^F165 is rescued by Cbl^{S.αTub84B.PJ} or Cbl^{L.αTub84B.PJ} as is the border cell migration defect seen in Cbl^F165 homozygous somatic clones. In contrast, Cbl^{C369A.SαTub84B.PJ} fails to rescue either of these phenotypes.

(Jekely et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9676

w¹¹¹⁸; Cbl^F165 P{neoFRT}80B e^s ca¹/TM3, Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Cbl^F165

(Wang et al., 2015, Johnson et al., 2012, Wang and Pai, 2011, Janssens et al., 2010, Chang et al., 2008, Miura et al., 2008, Bianco et al., 2007, Jekely et al., 2005)

D-cbl^F165

(Pai et al., 2006, Pai et al., 2000)

cbl^F165

(Bala Tannan et al., 2018, Ketosugbo et al., 2017, Pi et al., 2011, Epstein et al., 2009, Johnson and Cagan, 2009, Sullivan and Rubin, 2002)

d-cbl[F165]

(Callus and Mathey-Prevot, 2002)

dCbl^F165

(Truscott et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (18)

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