FlyBase Allele Report: Dmel\par-1[Δ-16]

General Information

Symbol

Dmel\par-1^Δ-16

Species

D. melanogaster

Name

FlyBase ID

FBal0121620

Feature type

allele

Associated gene

Dmel\par-1

Associated Insertion(s)

Carried in Construct

Also Known As

par-1^Δ16, par1^Δ16

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Cox et al., 2001)

Progenitor genotype

par-1^k06323

(Cox et al., 2001)

Cytology

Description

The complete kinase domain and most of the linker region of par-1 has been removed by a 16 kb deletion.

(Cox et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage (with par-1^k06323)

(Zhang et al., 2007)

abnormal neuroanatomy | pupal stage | somatic clone

(Herzmann et al., 2017)

female sterile

(Cox et al., 2001)

lethal | recessive

(Cox et al., 2001)

Phenotype Manifest In

border follicle cell, with Scer\GAL4^c306, par-1^{RNAi.UAS.cMDa}

(McDonald et al., 2008)

border follicle cell | somatic clone

(McDonald et al., 2008)

dendrite | pupal stage | somatic clone

(Herzmann et al., 2017)

follicle cell & microtubule | somatic clone

(Doerflinger et al., 2003)

follicle cell | somatic clone

(Benton and St. Johnston, 2003)

larval multidendritic class IV neuron | pupal stage | somatic clone

(Herzmann et al., 2017)

NMJ bouton (with par-1^k06323)

(Zhang et al., 2007)

nurse cell ring canal | germline clone

(Cox et al., 2001)

oocyte & pericentriolar material

(Cox et al., 2001)

oocyte | germline clone

(Cox et al., 2001)

subsynaptic reticulum (with par-1^k06323)

(Zhang et al., 2007)

Detailed Description

Statement

Reference

par-1^Δ-16 mutant class IV dendritic arborizing neuron MARCM clones display severe pruning defects at 18 h after pupariation.

(Herzmann et al., 2017)

Border follicle cell migration is disrupted in mosaic egg chambers containing homozygous follicle cell clones.

The severity of the border follicle cell migration defects seen in females expressing par-1^{dsRNA.Scer\UAS.cMDa} under the control of Scer\GAL4^c306 are increased if the flies are also heterozygous for par-1^Δ-16.

(McDonald et al., 2008)

A small fraction of par-1^Δ-16/par-1^k06323 animals survive to late larval stages. Some boutons at the neuromuscular junction of muscle 6/7 are smaller than normal and irregularly shaped in these mutant larvae, and the number of boutons is slightly, but significantly increased compared to normal. The subsynaptic reticulum (SSR) is expanded in the mutant type I boutons, with the overall SSR to bouton area ratio being higher than that of controls.

The frequency of the miniature excitatory junctional current is slightly increased in par-1^k06323/par-1^Δ-16 animals compared to controls, while the amplitude is not significantly altered. The amplitude of the evoked excitatory junctional current is increased in the mutant animals compared to controls, and the quantal content is also significantly increased.

(Zhang et al., 2007)

Homozygous follicle cell clones generated before the formation of the follicle cell epithelium show severe defects in epithelial organisation.

(Benton and St. Johnston, 2003)

Mutant follicle cells show an increase in the density of microtubules compared to their wild-type neighbours in mosaic egg chambers (when the egg chambers are fixed using an optimised procedure for preserving microtubules).

(Doerflinger et al., 2003)

Germ-line clones of par-1^Δ-16 yield no vitellogenic oocytes. Mutant females fail to produce any eggs. Mutant egg chambers contain 16 polyploid nurse cells, and no oocyte. The egg chambers are also blocked at stage 5 to 6 of oogenesis. The fusome develops normally in this egg chambers. However ring canal formation and organisation is disrupted. In mosaic egg chambers the posterior-most germ-cell (which would become the oocyte) does not show the characteristic cluster of the four largest ring canals. This cell also normally characterised by the presence of an active microtubule organisation centre (MTOC) when the developing egg chamber is in region 3 of the germarium. This is also not present in mutant egg chambers. In stage 2 mutant egg chambers, there is no apparent posterior accumulation of microtubules, but rather the microtubules remain evenly distributed within the mutant cyst. Finally in later stage mosaic egg chambers there remains no posterior accumulation of microtubules or visible MTOC formation. When homozygous mutant follicles cells surround wild-type germ-line cells, the normal development of 15 nurse cells and a single oocyte occurs. Within the mutant follicle cells there is a loss of microtubules (indicated by the abolishment of αtubulin expression). The cells also loses their characteristic columnar shape and appear irregular in size and shape. Secondly a disruption can be seen in the monolayer such that two follicle cells appear to be stacked on top of one another.

(Cox et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

par-1^Δ-16 has abnormal neuroanatomy | somatic clone | pupal stage phenotype, suppressible | partially by tau^MR22/tau[+]

(Herzmann et al., 2017)

Suppressor of

Statement

Reference

par-1^Δ-16/par-1[+] is a suppressor of abnormal neuroanatomy phenotype of dlg1⁷

(Zhang et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

par-1^Δ-16 has larval multidendritic class IV neuron | somatic clone | pupal stage phenotype, suppressible | partially by tau^MR22/tau[+]

(Herzmann et al., 2017)

Suppressor of

Statement

Reference

par-1^Δ-16/par-1[+] is a suppressor of eye phenotype of Hsap\DAPK1^UAS.cWa, Scer\GAL4^GMR.PF

(Wu et al., 2011)

par-1^Δ-16/par-1[+] is a suppressor of ommatidium phenotype of Hsap\DAPK1^UAS.cWa, Scer\GAL4^GMR.PF

(Wu et al., 2011)

par-1^Δ-16/par-1[+] is a suppressor | partially of photoreceptor neuron phenotype of Hsap\DAPK1^UAS.cWa, Scer\GAL4^GMR.PF

(Wu et al., 2011)

par-1^Δ-16/par-1[+] is a suppressor of eye phenotype of Hsap\DAPK1^K42A.UAS, Scer\GAL4^GMR.PF

(Wu et al., 2011)

par-1^Δ-16/par-1[+] is a suppressor of ommatidium phenotype of Hsap\DAPK1^K42A.UAS, Scer\GAL4^GMR.PF

(Wu et al., 2011)

par-1^Δ-16/par-1[+] is a suppressor | partially of photoreceptor neuron phenotype of Hsap\DAPK1^K42A.UAS, Scer\GAL4^GMR.PF

(Wu et al., 2011)

par-1^Δ-16/par-1[+] is a suppressor of NMJ bouton phenotype of dlg1⁷

(Zhang et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The pruning defects observed in class IV dendritic arborizing neuron par-1^Δ-16 mutant MARCM clones can be significantly suppressed by combination with a single copy of tau^MR22.

(Herzmann et al., 2017)

par-1^Δ-16/+ partially suppresses the decrease in the number of boutons at the neuromuscular junction which is seen in dlg1⁷ homozygotes.

(Zhang et al., 2007)

Xenogenetic Interactions

Statement

Reference

A par-1^Δ-16 heterozygous background alleviates the rough-eye phenotype seen upon expression of Hsap\DAPK1^{K42A.Scer\UAS} in the eye under the control of Scer\GAL4^GMR.PF. Photoreceptor neurons are partially rescued in these flies, albeit with abnormal numbers and arrangements.

A par-1^Δ-16 heterozygous background alleviates the rough-eye phenotype seen upon expression of Hsap\DAPK1^Scer\UAS.cWa in the eye under the control of Scer\GAL4^GMR.PF. Photoreceptor neurons are partially rescued in these flies, albeit with abnormal numbers and arrangements.

(Wu et al., 2011)

Complementation and Rescue Data

Fails to complement

par-1^70D3

(McDonald et al., 2008)

par-1^42D9

(McDonald et al., 2008)

par-1^36G4

(McDonald et al., 2008)

Partially rescued by

par-1^Δ-16 is partially rescued by par-1^{UAS.RR.Tag:FLAG}/Scer\GAL4^GMR57C10

(Herzmann et al., 2017)

par-1^Δ-16 is partially rescued by Scer\GAL4^GMR57C10/par-1^UAS.cSa

(Herzmann et al., 2017)

Not rescued by

par-1^Δ-16 is not rescued by Scer\GAL4^GMR57C10/par-1^KN.UAS

(Herzmann et al., 2017)

Comments

The pruning defects observed in class IV dendritic arborizing neuron par-1^Δ-16 mutant MARCM clones can be significantly rescued by expression of either par-1^Scer\UAS.cSa or par-1^{Scer\UAS.RR.T:Zzzz\FLAG} but not par-1^KN.Scer\UAS (with Scer\GAL4^GMR57C10) in the mutant clones.

(Herzmann et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

par-1^Δ-16

par-1^Δ16

(Herzmann et al., 2017, McDonald et al., 2008, Zhang et al., 2007, Benton and St. Johnston, 2003, Doerflinger et al., 2003)

par1^Δ16

(Walther et al., 2016, Wang et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (9)

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