Homozygous ab1C olfactory receptor neurons (ORNs) generated using MARCM show normal responses to CO[[2]] in 6 day old flies. A decrease in CO[[2]] response is observed in these neurons in 20 day old flies, at all concentrations of CO[[2]] tested.

Homozygous ab3A ORNs generated using MARCM show a normal odour response to low concentrations of either methyl butyrate (0.05% and lower) or butyric acid (1% and lower). However, at near saturating concentrations, there is a small decrease in the odour response for both these odorants.

Uptake of 40-80mM trehalose solution is significantly lower in G-sα60A^R60/+ flies than in wild-type controls. This effect is not seen with lower concentrations of trehalose.

Gαs^R60 mutant flies show resistance to V. cholerae infection compared to controls.

Somatic clones of G-sα60A^R60 homozygous cells persist in the adult wing blade after surrounding heterozygous or wild-type cells have died.

Low-frequency nerve stimulation at 0.5 mM Ca²⁺ evokes robust synaptic currents in G-sα60A^R60 embryos. However, at 0.2 mM Ca²⁺ nerve stimulation often fails to evoke synaptic currents in homozygous G-sα60A^R60 embryos while currents are evoked in G-sα60A^R60/+ embryos. G-sα60A^R60 mutants show minimal synaptic facilitation during tetanic stimulation and lack of post-tetanic potentiation, compared to G-sα60A^R60/+ embryos. Additionally, during and after tetanus, asynchronous transmitter release is not enhanced to the same levels in G-sα60A^R60 homozygotes as in G-sα60A^R60/+ heterozygotes. Miniature synaptic currents (mSCs) are smaller in amplitude and less frequent in G-sα60A^R60 embryos when tested in saline with normal concentrations of K⁺ than in heterozygotes. In high concentrations of K⁺ saline, G-sα60A^R60 embryos produce less frequent mSCs with smaller average amplitudes and the amplitude distribution is skewed toward larger amplitudes compared to G-sα60A^R60/+ embryos. The mean amplitude of glutamate-induced inward currents is not different in G-sα60A^R60 homozygotes and heterozygotes, showing that the total number of postsynaptic receptors is not different. Additionally, the unitary glutamate receptor channel current is not different and the pre-synaptic receptors for metabotropic glutamate and octopamine do not have an altered response in G-sα60A^R60 embryos.

38% of homozygous embryos die (the percentage of embryos dying is not increased if they are derived from females carrying homozygous germ-line clones), the remaining homozygous larvae die either as they hatch or shortly after hatching. Larvae that hatch show little or no movement and do not grow. Ovaries of females carrying homozygous germ-line clones appear normal. 11% of G-sα60A^R60/G-sα60A^R19 embryos die. 44% of G-sα60A^R60/G-sα60A^R19 embryos derived from females carrying G-sα60A^R60 homozygous germ-line clones die. 27% of G-sα60A^R60/Df(2R)or-BR11 embryos die. 48% of G-sα60A^R60/Df(2R)or-BR11 embryos derived from females carrying G-sα60A^R60 homozygous germ-line clones die. G-sα60A^R60/G-sα60A^R19 embryos derived from females carrying G-sα60A^R60 or G-sα60A^R19 homozygous germ-line clones do not have cuticle defects.

Gαs^R60/G-salpha60A[+] is a suppressor of scutellar bristle | increased number phenotype of Chi^e5.5

Gαs^R60/G-salpha60A[+] is a suppressor of scutellar bristle | increased number phenotype of Ssdp^L7