FlyBase Allele Report: Dmel\mask[10.22]

General Information

Symbol

Dmel\mask^10.22

Species

D. melanogaster

Name

FlyBase ID

FBal0134057

Feature type

allele

Associated gene

Dmel\mask

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Sidor et al., 2019)

loss of function allele

(Smith et al., 2002)

Mutagen

ethyl methanesulfonate

(Smith et al., 2002)

Progenitor genotype

Cytology

Description

Amino acid replacement: K873term.

(Smith et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:24,237,872..24,237,872 [+]

Nucleotide change:

A24237872T

Amino acid change:

Reported amino acid change:

K873term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Smith et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by park²⁵, park^Δ21

(Zhu et al., 2015)

modeled by Pink1^B9

(Zhu et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

park[25]/park[Δ21] transheterozygotes were used as the disease model.

(Zhu et al., 2015)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage | somatic clone

(Smith et al., 2002)

decreased cell number | larval stage | somatic clone

(Smith et al., 2002)

increased cell death | larval stage | somatic clone

(Smith et al., 2002)

increased cell death | recessive | somatic clone

(Smith et al., 2002)

viable (with mask^EY13048)

(Zhu et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

mask^10.22/Df(3R)BSC317 wandering third instar larvae have more autophagosomes in somatic muscles.

(Zhu et al., 2017)

mask^10.22/Df(3R)BSC317 mutant third instar larvae display reduced connectivity of the mitochondrial network as well as defects in the subcellular distribution of mitochondria in a body wall muscle.

(Zhu et al., 2015)

Homozygous clones in the eye-antennal disc are significantly smaller than wild-type twin spot clones. The mutant cells do not appear to be significantly smaller than wild-type cells. The mutant cells show an increase in apoptosis compared to the wild-type cells. Some cells within clones in the third larval instar eye disc begin photoreceptor differentiation, but the ommatidia within clones contain less than the normal number of photoreceptor cells. Adult flies show loss of outer and inner photoreceptor cells within many clonal ommatidia. Analysis of mosaic ommatidia containing both wild-type and homozygous cells indicates that there is a partial requirement for mask function for the differentiation of R1-R8 photoreceptor cells. The R7 or R6 cell is never homozygous in mosaic ommatidia and the number of homozygous R1 cells in mosaic ommatidia is significantly reduced. Large homozygous clones in the eye (generated using the "ey-flp" method) can proliferate and form much of the eye. However, many photoreceptors, including the majority of R7 cells, fail to differentiate. Eggs derived from females containing homozygous follicle cells have fused or closely apposed dorsal appendages.

(Smith et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

mask^10.22 is an enhancer of visible phenotype of Ras85D^N17.hs.2sev

(Smith et al., 2002)

mask[+]/mask^10.22 is an enhancer of increased cell death phenotype of csw^CS.hs.2sev

(Smith et al., 2002)

mask[+]/mask^10.22 is an enhancer of visible phenotype of csw^CS.hs.2sev

(Smith et al., 2002)

Suppressor of

Statement

Reference

mask[+]/mask^10.22 is a suppressor | partially of abnormal flight phenotype of park^Δ21/park²⁵

(Zhu et al., 2015)

mask^EY13048/mask^10.22 is a suppressor | partially of abnormal flight phenotype of park^Δ21/park²⁵

(Zhu et al., 2015)

mask^EY13048/mask^10.22 is a suppressor | partially of abnormal flight phenotype of Pink1^B9

(Zhu et al., 2015)

mask[+]/mask^10.22 is a suppressor | partially of abnormal flight phenotype of Pink1^B9

(Zhu et al., 2015)

mask^10.22 is a suppressor of visible phenotype of Ras85D^V12.sev

(Smith et al., 2002)

mask[+]/mask^10.22 is a suppressor of visible phenotype of csw::Src64B^hs.2sev

(Smith et al., 2002)

mask[+]/mask^10.22 is a suppressor of visible phenotype of rl^D334N.hs.sev

(Smith et al., 2002)

mask[+]/mask^10.22 is a suppressor of visible phenotype of sev^S11.Tag:MYC

(Smith et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

mask^10.22, park^Δ21/park²⁵ has adult external thorax phenotype, suppressible by Atg18a^KG03090/Atg18a[+]

(Zhu et al., 2015)

Enhancer of

Statement

Reference

mask^10.22 is an enhancer of ommatidium phenotype of Ras85D^N17.hs.2sev

(Smith et al., 2002)

mask[+]/mask^10.22 is an enhancer of eye disc phenotype of csw^CS.hs.2sev

(Smith et al., 2002)

mask[+]/mask^10.22 is an enhancer of ommatidium phenotype of csw^CS.hs.2sev

(Smith et al., 2002)

Suppressor of

Statement

Reference

mask[+]/mask^10.22 is a suppressor | partially of adult external thorax phenotype of Pink1^B9

(Zhu et al., 2015)

mask^EY13048/mask^10.22 is a suppressor | partially of adult external thorax phenotype of Pink1^B9

(Zhu et al., 2015)

mask^EY13048/mask^10.22 is a suppressor | partially of mitochondrion | adult stage phenotype of Pink1^B9

(Zhu et al., 2015)

mask^EY13048/mask^10.22 is a suppressor | partially of indirect flight muscle cell phenotype of Pink1^B9

(Zhu et al., 2015)

mask^EY13048/mask^10.22 is a suppressor of mitochondrion | third instar larval stage phenotype of Pink1^B9

(Zhu et al., 2015)

mask^EY13048/mask^10.22 is a suppressor of embryonic/larval somatic muscle cell | third instar larval stage phenotype of Pink1^B9

(Zhu et al., 2015)

mask[+]/mask^10.22 is a suppressor | partially of adult external thorax phenotype of park^Δ21/park²⁵

(Zhu et al., 2015)

mask^EY13048/mask^10.22 is a suppressor | partially of adult external thorax phenotype of park^Δ21/park²⁵

(Zhu et al., 2015)

mask^EY13048/mask^10.22 is a suppressor | partially of mitochondrion | adult stage phenotype of park^Δ21/park²⁵

(Zhu et al., 2015)

mask^EY13048/mask^10.22 is a suppressor | partially of indirect flight muscle cell phenotype of park^Δ21/park²⁵

(Zhu et al., 2015)

mask^EY13048/mask^10.22 is a suppressor of mitochondrion | third instar larval stage phenotype of park^Δ21/park²⁵

(Zhu et al., 2015)

mask^EY13048/mask^10.22 is a suppressor of embryonic/larval somatic muscle cell | third instar larval stage phenotype of park^Δ21/park²⁵

(Zhu et al., 2015)

mask^10.22 is a suppressor of ommatidium phenotype of Ras85D^V12.sev

(Smith et al., 2002)

mask[+]/mask^10.22 is a suppressor of photoreceptor cell R7 | ectopic phenotype of csw::Src64B^hs.2sev

(Smith et al., 2002)

mask[+]/mask^10.22 is a suppressor of photoreceptor cell R7 | ectopic phenotype of rl^D334N.hs.sev

(Smith et al., 2002)

mask[+]/mask^10.22 is a suppressor of photoreceptor cell R7 | ectopic phenotype of sev^S11.Tag:MYC

(Smith et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The indentations on the thorax of adult males hemizygous for Pink1^B9 as well as their reduced ability to fly can be partially suppressed, in a dose-dependent manner, by combination with mask^10.22/+ or mask^10.22/mask^EY13048 alleles, which leads to significantly lower penetrance of the thoracic phenotype and increased flight ability compared to Pink1^B9 mutants alone.

The disturbed morphology of both mitochondria and indirect flight muscles characteristic for adult Pink1^B9 hemizygote males is substantially suppressed by combination with mask^10.22/+ or mask^10.22/mask^EY13048 alleles.

The indentations on the thorax of adult park²⁵/park^Δ21 transheterozygotes as well as their reduced ability to fly can be partially suppressed, in a dose-dependent manner, by combination with mask^10.22/+ or mask^10.22/mask^EY13048 alleles, which leads to significantly lower penetrance of the thoracic phenotype and increased flight ability compared to park²⁵/park^Δ21 mutants alone.

The disturbed morphology of both mitochondria and indirect flight muscles characteristic for adult park²⁵/park^Δ21 transheterozygotes is substantially suppressed by combination with mask^10.22/+ or mask^10.22/mask^EY13048 alleles.

The enlarged or aggregated mitochondria phenotype observed in the body wall muscles of park²⁵/park^Δ21 transheterozygote third instar larvae is suppressed by combination with mask^10.22/mask^EY13048 alleles.

The enlarged or aggregated mitochondria phenotype observed in the body wall muscles of Pink1^B9 hemizygous male third instar larvae is suppressed either by combination with mask^10.22/mask^EY13048 alleles or by ectopic expression of park^Scer\UAS.cGa under the control of Scer\GAL4^how-24B.

(Zhu et al., 2015)

Enhances the csw^CS.hs.2sev rough eye phenotype. mask^10.22 dominantly suppresses the ectopic R7 phenotype caused by sev^{S11.T:Hsap\MYC}; the double mutant flies have an average of 1.8 ectopic R7 cells per ommatidium. mask^10.22 dominantly suppresses the ectopic R7 phenotype caused by csw::Src64B^hs.2sev; the double mutant flies have an average of 0.6 ectopic R7 cells per ommatidium. One copy of mask^10.22 weakly suppresses the Ras85D^V12.sev rough eye phenotype. One copy of mask^10.22 weakly enhances the Ras85D^N17.hs.2sev rough eye phenotype. The number of apoptotic cells in csw^CS.hs.2sev third larval instar eye discs is increased, both anterior and posterior to the morphogenetic furrow if the flies are also carrying one copy of mask^10.22. The rl^D334N.hs.sev ectopic R7 cell phenotype is partially suppressed by mask^10.22/+; the double mutant flies have an average of 1.2 ectopic R7 cells per ommatidium.

(Smith et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mask^10.22/Df(3R)BSC317 is rescued by mask^UAS.cZa

(Zhu et al., 2017)

mask^10.22/Df(3R)BSC317 is rescued by mask^UAS.cZa/Scer\GAL4^how-24B

(Zhu et al., 2015)

Comments

Expressing mask^UAS.cZa under the control of Scer\GAL4^how-24B rescues the over-accumulation of autophagosomes and increases the amount of autolysosomes in somatic muscles from mask^10.22/Df(3R)BSC317 wandering third instar larvae.

(Zhu et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mask^10.22

(Martinez et al., 2021, Sidor et al., 2019, Zhu et al., 2015, Sidor et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (6)

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