FlyBase Allele Report: Dmel\lgs[20F]

General Information

Symbol

Dmel\lgs^20F

Species

D. melanogaster

Name

FlyBase ID

FBal0135670

Feature type

allele

Associated gene

Dmel\lgs

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Kramps et al., 2002)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q384term.

(Kramps et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

4:440,969..440,969 [-]

Nucleotide change:

C440969T

Amino acid change:

Q389term | lgs-PA

Reported amino acid change:

Q384term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Reported amino acid change refers to shorter CDS.

(Kramps et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

denticle | ectopic

(Hoffmans and Basler, 2004)

embryonic/first instar larval cuticle

(Hoffmans and Basler, 2004)

embryonic/first instar larval cuticle | maternal effect

(Townsley et al., 2004)

imaginal disc

(Kramps et al., 2002)

Detailed Description

Statement

Reference

lgs^20F homozygous embryos make a shortened cuticle with an excess of ventral denticles at the expense of naked cuticle.

(Hoffmans and Basler, 2004)

lgs^20F mutant embryos that lack maternal and zygotic lgs function display a cuticle denticle lawn that is characteristic of a complete loss of wg signalling.

(Townsley et al., 2004)

Discs from mutant larvae are abnormally small. Homozygous mutant somatic clones can be made by rescuing lgs^20F with lgs^αTub84B.PK and FLPing out lgs to create clones that are lgs^20F and Scer\GAL4^αTub84B.PK. When this is done, clones typically undergo only a few divisions after they are generated in the presumptive wing blade, then the mutant cells stop proliferating and either die or are actively eliminated from the disc epithelium.

(Kramps et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

lgs^20F has lethal | larval stage phenotype, suppressible by lgs::pygo^{ΔPHD.HD2.αTub84B}

(Kramps et al., 2002)

lgs^20F has lethal | larval stage phenotype, suppressible by Hsap\BCL9^αTub84B.PK

(Kramps et al., 2002)

lgs^20F has lethal | segment polarity expression pattern | germline clone | larval stage phenotype, suppressible by Hsap\BCL9^αTub84B.PK

(Kramps et al., 2002)

NOT suppressed by

Statement

Reference

lgs^20F has lethal | segment polarity expression pattern | germline clone | larval stage phenotype, non-suppressible by sgg^M11

(Kramps et al., 2002)

Suppressor of

Statement

Reference

lgs[+]/lgs^20F is a suppressor of visible phenotype of Chi^e5.5

(van Tienen et al., 2017)

lgs[+]/lgs^20F is a suppressor of visible phenotype of arm^S56F.GMR

(van Tienen et al., 2017)

lgs^20F is a suppressor of visible phenotype of wg^hs.2sev

(Kramps et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

lgs^20F has embryonic/first instar larval cuticle | maternal effect phenotype, suppressible by Scer\GAL4^da.G32/arm^{UAS.Tag:MYC,Tag:NLS(SV40-largeT)}

(Townsley et al., 2004)

Suppressor of

Statement

Reference

lgs[+]/lgs^20F is a suppressor of wing phenotype of Chi^e5.5

(van Tienen et al., 2017)

lgs[+]/lgs^20F is a suppressor of eye phenotype of arm^S56F.GMR

(van Tienen et al., 2017)

lgs^20F is a suppressor of eye phenotype of wg^hs.2sev

(Kramps et al., 2002)

lgs^20F is a suppressor of ommatidium phenotype of wg^hs.2sev

(Kramps et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The wing notch phenotype seen in Chi^e5.5/+ mutants is partially suppressed by the presence of lgs^20F/+.

lgs^20F/+ suppresses the rough eye phenotype seen in flies expressing arm^S56F.GMR.

(van Tienen et al., 2017)

Overexpression of arm^{Scer\UAS.T:Hsap\MYC,T:SV40\nls2}, under the regulation of Scer\GAL4^da.G32 in lgs^20F mutants which lack both maternal and zygotic lgs function converts the lgs^20F denticle lawn into an entirely naked cuticle along the ventral midline.

(Townsley et al., 2004)

lgs^20F, sgg^M11 homozygous mutant germ-line clones are indistinguishable from lgs^20F germ-line clones.

(Kramps et al., 2002)

Xenogenetic Interactions

Statement

Reference

The addition of Hsap\BCL9^αTub84B.PK to lgs^20F animals leads to a partial suppression of the larval lethal phenotype, though 80% of animals die as pharate adults. lgs^20F embryos lacking both maternal and zygotic lgs are fully rescued by maternal expression of Hsap\BCL9^αTub84B.PK.

(Kramps et al., 2002)

Complementation and Rescue Data

Rescued by

lgs^20F is rescued by lgs^{1-583.αTub84B}

(Kramps et al., 2002)

Partially rescued by

lgs^20F is partially rescued by lgs^{HD1.HA3.HD2.αTub84B.Tag:HA}

(Kramps et al., 2002)

lgs^20F is partially rescued by lgs^{HD1.Gal11.HD2.αTub84B}

(Kramps et al., 2002)

Comments

Images (0)