FlyBase Allele Report: Dmel\osk[A87]

General Information

Symbol

Dmel\osk^A87

Species

D. melanogaster

Name

FlyBase ID

FBal0141009

Feature type

allele

Associated gene

Dmel\osk

Associated Insertion(s)

ZAM{}osk^A87

Carried in Construct

Key Links

Allele class

loss of function allele

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Kanke and Macdonald, 2015)

loss of function allele

(Jenny et al., 2006, Vanzo and Ephrussi, 2002)

Mutagen

Progenitor genotype

Associated Insertion(s)

ZAM{}osk^A87

Cytology

Description

Contains a ZAM element in the first exon of the osk gene.

(Jenny et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

embryonic/larval segmentation phenotype | maternal effect (with Df(3R)osk)

(Kanke and Macdonald, 2015)

embryonic/larval segmentation phenotype | maternal effect (with Df(3R)osk), with osk^{M1R.INV121-182.Tag:HA}

(Kanke and Macdonald, 2015)

embryonic/larval segmentation phenotype | maternal effect (with Df(3R)osk), with osk^{M1R.Δ91-120.Tag:HA}

(Kanke and Macdonald, 2015)

embryonic/larval segmentation phenotype | maternal effect (with Df(3R)osk), with osk^{M1R.Δ118-135.Tag:HA}

(Kanke and Macdonald, 2015)

embryonic/larval segmentation phenotype | maternal effect (with Df(3R)osk), with osk^{M1R.Δ121-150.Tag:HA}

(Kanke and Macdonald, 2015)

female sterile (with Df(3R)p-XT103)

(Vazquez-Pianzola et al., 2011, Jenny et al., 2006)

lethal - all die during embryonic stage | maternal effect (with osk¹)

(Szuperak et al., 2005)

some die during embryonic stage | maternal effect (with osk¹)

(Szuperak et al., 2005)

Phenotype Manifest In

egg chamber (with Df(3R)p-XT103)

(Jenny et al., 2006)

embryo, with Df(3R)p-XT103/osk^+tMa

(Morais-de-Sá et al., 2013)

embryo (with Df(3R)p-XT103), with osk^+tMa

(Morais-de-Sá et al., 2013)

embryonic/larval cuticle | maternal effect (with Df(3R)osk)

(Kanke and Macdonald, 2015)

embryonic/larval cuticle | maternal effect (with Df(3R)osk), with osk^{M1R.INV121-182.Tag:HA}

(Kanke and Macdonald, 2015)

embryonic/larval cuticle | maternal effect (with Df(3R)osk), with osk^{M1R.Δ91-120.Tag:HA}

(Kanke and Macdonald, 2015)

embryonic/larval cuticle | maternal effect (with Df(3R)osk), with osk^{M1R.Δ118-135.Tag:HA}

(Kanke and Macdonald, 2015)

embryonic/larval cuticle | maternal effect (with Df(3R)osk), with osk^{M1R.Δ121-150.Tag:HA}

(Kanke and Macdonald, 2015)

embryonic abdomen, with osk^mM1mM2

(Jenny et al., 2006)

embryonic abdomen, with osk^mM1SLmM2

(Jenny et al., 2006)

embryonic abdomen (with Df(3R)p-XT103), with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, osk^3'UTR.UASp

(Jenny et al., 2006)

embryonic abdomen (with osk¹)

(Jenny et al., 2006)

embryonic abdomen (with osk²)

(Jenny et al., 2006)

embryonic abdomen (with osk⁹)

(Jenny et al., 2006)

embryonic abdomen (with osk^mM1mM2)

(Jenny et al., 2006)

embryonic abdomen (with osk^mM1SLmM2)

(Jenny et al., 2006)

embryonic abdominal segment, with Df(3R)p-XT103/osk^+tMa

(Morais-de-Sá et al., 2013)

embryonic abdominal segment (with Df(3R)p-XT103), with osk^+tMa

(Morais-de-Sá et al., 2013)

germline cell (with Df(3R)p-XT103), with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, osk^3'UTR.UASp

(Jenny et al., 2006)

germline cell (with osk¹)

(Jenny et al., 2006)

germline cell (with osk²)

(Jenny et al., 2006)

germline cell (with osk⁹)

(Jenny et al., 2006)

karyosome (with Df(3R)p-XT103)

(Jenny et al., 2006)

oocyte (with Df(3R)p-XT103)

(Vazquez-Pianzola et al., 2011)

Detailed Description

Statement

Reference

Segmentation defects are observed in embryos from osk^A87/Df(3R)osk mothers expressing osk^{M1R.Δ118-135.T:Ivir\HA1}.

Segmentation defects are observed in embryos from osk^A87/Df(3R)osk mothers expressing osk^{M1R.Δ121-150.T:Ivir\HA1}.

Segmentation defects are observed in embryos from osk^A87/Df(3R)osk mothers expressing osk^{M1R.Δ91-120.T:Ivir\HA1}.

Segmentation defects are observed in embryos from osk^A87/Df(3R)osk mothers expressing osk^{M1R.INV121-182.T:Ivir\HA1}.

(Kanke and Macdonald, 2015)

Expression of osk^t.WT in osk¹/Df(3R)p-XT103 mutant mothers results in embryos containing an average of 24 pole cells, as in wild-type.

One copy of osk^t.WT suppresses the arrest in oogenesis found in osk^A87/Df(3R)p-XT103. However, 10% of the resulting embryos are missing abdominal segments, displaying a weak posterior group phenotype.

(Morais-de-Sá et al., 2013)

osk^A87/Df(3R)p-XT103 females fail to lay eggs and are sterile owing to the early arrest of oogenesis.

(Vazquez-Pianzola et al., 2011)

osk¹/osk^A87, osk²/osk^A87 and osk⁹/osk^A87 transheterozygous embryos show the 'posterior group' phenotype as they lack an abdomen and germline.

osk^A87/Df(3R)p-XT103 females fail to lay eggs although oocytes are determined and begin to develop, but then arrest. Defects become evident in the egg chambers at stage 2, when fragmentation of the normally compact karyosome is observed. osk^A87/Df(3R)p-XT103 egg chambers continue to develop until stage 7, when they begin to degenerate.

osk^A87/Df(3R)p-XT103 females that carry either the osk^mM1mM2 or the osk^mM1SLmM2 transgene are able to lay eggs but the resulting embryos lack abdomens.

Embryos produced by osk^A87/Df(3R)p-XT103 females that express osk^{3'UTR.Scer\UAS.P\T}, under the control of both Scer\GAL4^{otu.T:Hsim\VP16} and Scer\GAL4^{nos.UTR.T:Hsim\VP16}, show the 'posterior group' phenotype.

(Jenny et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

osk[+]/osk^A87 is a suppressor | maternal effect | partially of some die during embryonic stage | maternal effect | cold sensitive phenotype of klar^YG3

(Gaspar et al., 2014)

osk[+]/osk^A87 is a suppressor | maternal effect | partially of some die during embryonic stage | maternal effect phenotype of klar^YG3/klar¹

(Gaspar et al., 2014)

Other

Statement

Reference

Pym^{UASp.Tag:FLAG}, Scer\GAL4^{VP16.nanos.UTR}, osk[+]/osk^A87 has female semi-sterile phenotype

(Ghosh et al., 2014)

Pym^{UASp.Tag:FLAG}, Scer\GAL4^{VP16.nanos.UTR}, osk[+]/osk^A87 has grandchildless phenotype

(Ghosh et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

osk[+]/osk^A87 is a suppressor of embryonic head phenotype of mira^{αTub67C.mGFP6}

(Chang et al., 2011)

Other

Statement

Reference

osk[+]/osk^A87, pAbp^37-2 has embryo phenotype

(Vazquez-Pianzola et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The bicaudal phenotype of embryos derived from mira^{αTub67C.T:Avic\GFP-m6}/+ females is almost completely suppressed (99.2%) if the females are also heterozygous for osk^A87. Although these embryos form normal heads, only 0.6% of them hatch.

(Chang et al., 2011)

Females double heterozygous for pAbp^37-2 and osk^A87 produce much more frequently embryos with strong posterior phenotypes than females heterozygous for each of these mutations alone.

(Vazquez-Pianzola et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

osk⁹

(Jenny et al., 2006)

osk²

(Jenny et al., 2006)

osk¹

(Jenny et al., 2006)

Rescued by

osk^A87/Df(3R)osk is rescued by osk^Tag:HA

(Kanke and Macdonald, 2015)

osk^A87/Df(3R)p-XT103 is rescued by osk^{3'UTR.UASp.cVPa}

(Vazquez-Pianzola et al., 2011)

osk^A87/Df(3R)osk is rescued by osk^+t8

(Reveal et al., 2010)

osk^A87/Df(3R)osk is rescued by osk^BRE.AB-

(Reveal et al., 2010)

osk^A87/Df(3R)p-XT103 is rescued by osk^+t6.45

(Jenny et al., 2006)

osk^A87/Df(3R)p-XT103 is rescued by osk^M1L

(Jenny et al., 2006)

osk^A87/Df(3R)p-XT103 is rescued by osk^M139L

(Jenny et al., 2006)

osk^A87/Df(3R)p-XT103 is rescued by Scer\GAL4^VP16.otu/Scer\GAL4^{VP16.nanos.UTR}/osk^UASp.cJa

(Jenny et al., 2006)

osk^A87/Df(3R)p-XT103 is rescued by Scer\GAL4^VP16.otu/Scer\GAL4^{VP16.nanos.UTR}/osk^Δi.123.UASp

(Jenny et al., 2006)

osk^A87/Df(3R)p-XT103 is rescued by osk^TTgAY.B

(Munro et al., 2006)

osk^A87/Df(3R)p-XT103 is rescued by osk^13TTTAY

(Munro et al., 2006)

osk^A87/Df(3R)p-XT103 is rescued by Scer\GAL4^VP16.otu/Scer\GAL4^{VP16.nanos.UTR}/osk^UASp.primary

(Hachet and Ephrussi, 2004)

Partially rescued by

osk^A87/Df(3R)osk is partially rescued by osk^M1R.Tag:HA

(Kanke and Macdonald, 2015)

osk^A87/Df(3R)osk is partially rescued by osk^{M1R.Δ151-180.Tag:HA}

(Kanke and Macdonald, 2015)

osk^A87/Df(3R)osk is partially rescued by osk^{M1R.Δ40-84.Δ196-423.Tag:HA}

(Kanke and Macdonald, 2015)

osk^A87/Df(3R)osk is partially rescued by osk^{Δ121-150.Tag:HA}

(Kanke and Macdonald, 2015)

osk^A87/Df(3R)p-XT103 is partially rescued by osk^S244A.S248A

(Morais-de-Sá et al., 2013)

osk^A87/Df(3R)p-XT103 is partially rescued by Scer\GAL4^VP16.otu/Scer\GAL4^{VP16.nanos.UTR}/osk^3'UTR.UASp

(Jenny et al., 2006)

osk^A87/Df(3R)p-XT103 is partially rescued by osk^mM1mM2

(Jenny et al., 2006)

osk^A87/Df(3R)p-XT103 is partially rescued by osk^mM1SLmM2

(Jenny et al., 2006)

Not rescued by

osk^A87/Df(3R)osk is not rescued by osk^{M1R.INV121-182.Tag:HA}

(Kanke and Macdonald, 2015)

osk^A87/Df(3R)osk is not rescued by osk^{M1R.Δ91-120.Tag:HA}

(Kanke and Macdonald, 2015)

osk^A87/Df(3R)osk is not rescued by osk^{M1R.Δ118-135.Tag:HA}

(Kanke and Macdonald, 2015)

osk^A87/Df(3R)osk is not rescued by osk^{M1R.Δ121-150.Tag:HA}

(Kanke and Macdonald, 2015)

osk^A87/Df(3R)p-XT103 is not rescued by osk^{3'UTR.ΔAR-a.UASp}

(Vazquez-Pianzola et al., 2011)

osk^A87/Df(3R)p-XT103 is not rescued by osk^{3'UTR.ΔAR-b.UASp}

(Vazquez-Pianzola et al., 2011)

osk^A87/Df(3R)osk is not rescued by osk^BRE.C-

(Reveal et al., 2010)

osk^A87/Df(3R)p-XT103 is not rescued by osk^{fs(1)K10.3'UTR.UAS}/Scer\GAL4^VP16.otu/Scer\GAL4^{VP16.nanos.UTR}

(Jenny et al., 2006)

osk^A87/Df(3R)p-XT103 is not rescued by osk^TTgAY.A

(Munro et al., 2006)

osk^A87/Df(3R)p-XT103 is not rescued by osk^TTgAY.C

(Munro et al., 2006)

osk^A87/Df(3R)p-XT103 is not rescued by osk^TTgAY.D

(Munro et al., 2006)

osk^A87/Df(3R)p-XT103 is not rescued by Scer\GAL4^VP16.otu/Scer\GAL4^{VP16.nanos.UTR}/osk^{UASp.Δi.1.2.3}

(Hachet and Ephrussi, 2004)

Comments

Almost all embryos from osk^A87/Df(3R)osk mothers expressing osk^{Δ121-150.T:Ivir\HA1} display normal segmentation.

Embryos from osk^A87/Df(3R)osk mothers expressing osk^{M1R.Δ40-84.Δ196-423.T:Ivir\HA1} display normal segmentation.

Almost all embryos from osk^A87/Df(3R)osk mothers expressing osk^{M1R.Δ40-84.Δ196-423.T:Ivir\HA1} display normal segmentation.

Segmentation defects are observed in embryos from osk^A87/Df(3R)osk mothers expressing osk^{M1R.Δ118-135.T:Ivir\HA1}.

Almost all embryos from osk^A87/Df(3R)osk mothers expressing osk^{M1R.Δ151-180.T:Ivir\HA1} display normal segmentation.

Segmentation defects are observed in embryos from osk^A87/Df(3R)osk mothers expressing osk^{M1R.Δ121-150.T:Ivir\HA1}.

Segmentation defects are observed in embryos from osk^A87/Df(3R)osk mothers expressing osk^{M1R.Δ91-120.T:Ivir\HA1}.

Segmentation defects are observed in embryos from osk^A87/Df(3R)osk mothers expressing osk^{M1R.INV121-182.T:Ivir\HA1}.

Almost all embryos from osk^A87/Df(3R)osk mothers expressing osk^{M1R.T:Ivir\HA1} display normal segmentation.

(Kanke and Macdonald, 2015)

Expression of osk^{3'UTR.attB.Scer\UAS.P\T} driven by a combination of Scer\GAL4^{nos.UTR.T:Hsim\VP16} and Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} is sufficient to rescue the early oogenesis arrest and egg-less phenotype of osk^A87/Df(3R)p-XT103 females.

Expression of osk^{3'UTR.ΔAR-a.Scer\UAS.P\T} driven by a combination of Scer\GAL4^{nos.UTR.T:Hsim\VP16} and Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} fails to rescue the egg-less phenotype of osk^A87/Df(3R)p-XT103 females and egg chambers do not develop beyond stage 7.

Expression of osk^{3'UTR.ΔAR-b.Scer\UAS.P\T} driven by a combination of Scer\GAL4^{nos.UTR.T:Hsim\VP16} and Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} fails to rescue the egg-less phenotype of osk^A87/Df(3R)p-XT103 females and egg chambers do not develop beyond stage 7.

(Vazquez-Pianzola et al., 2011)

The cuticle defects of embryos derived from osk^A87/Df(3R)osk females are rescued by osk^+t8.

osk^BRE.AB- almost completely rescues the cuticle defects of embryos derived from osk^A87/Df(3R)osk females; 96% have wild-type cuticles, while 4% have head defects.

83% of embryos derived from osk^A87/Df(3R)osk females carrying osk^BRE.C- have 0-1 abdominal segments, and 14% have 2-7 abdominal segments.

(Reveal et al., 2010)

Expression of the either the osk^mM1mM2 or the osk^mM1SLmM2 transgene rescues the egg-less phenotype of osk^A87/Df(3R)p-XT103 females but the embryos produced lack an abdomen.

Expression of osk^{Scer\UAS.P\T.cJa}, under the control of both Scer\GAL4^{otu.T:Hsim\VP16} and Scer\GAL4^{nos.UTR.T:Hsim\VP16}, rescues the early oogenesis arrest of osk^A87/Df(3R)p-XT103 females, as well as the 'posterior group' phenotype of the progeny (lack of abdomen and germline).

Expression of the intronless osk^{Δi.123.Scer\UAS.P\T}, under the control of both Scer\GAL4^{otu.T:Hsim\VP16} and Scer\GAL4^{nos.UTR.T:Hsim\VP16}, rescues the early oogenesis arrest of osk^A87/Df(3R)p-XT103 females.

Expression of osk^{3'UTR.Scer\UAS.P\T}, under the control of both Scer\GAL4^{otu.T:Hsim\VP16} and Scer\GAL4^{nos.UTR.T:Hsim\VP16}, rescues the early oogenesis and egg-less phenotype of osk^A87/Df(3R)p-XT103 females and the karyosome defect. However, the 'posterior group' phenotype of the embryos produced from these females is not rescued.

(Jenny et al., 2006)

osk^13TTTAY rescues the stage 6 oocyte arrest phenotype of osk^A87/Df(3R)p-XT103 females.

osk^13TTgAY rescues the stage 6 oocyte arrest phenotype of osk^A87/Df(3R)p-XT103 females. However, embryos derived from osk^A87/Df(3R)p-XT103;osk^13TTgAY mothers display a fully penetrant osk mutant maternal-effect phenotype in which the abdomen fails to form.

Expression of osk^TTgAY.A, osk^TTgAY.C or osk^TTgAY.D fails to rescue the osk RNA and protein localisation defects observed in osk^A87/Df(3R)p-XT103 oocytes.

Expression of the osk^TTgAY.B transgene fully rescues the osk RNA and protein localisation defects observed in osk^A87/Df(3R)p-XT103 oocytes.

(Munro et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

M. Erdelyi D. St Johnston A. Ephrussi

(Vanzo and Ephrussi, 2002)

Mutagen comment: this allele was discovered in an EMS mutagenesis screen, although it was found to have a ZAM insertion.

(Jenny et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Name Synonyms

Secondary FlyBase IDs

References (33)

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