FB2024_03 , released June 25, 2024
Allele: Dmel\Atx2UAS.cSa
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General Information
Symbol
Dmel\Atx2UAS.cSa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Satterfield
FlyBase ID
FBal0141943
Feature type
allele
Associated gene
Dmel\Atx2
Associated Insertion(s)
Carried in Construct
P{UAS-Atx2.S}
Also Known As
UAS-Atx2, UAS-Datx2
Key Links
Transgenic product class
cDNA
(Satterfield et al., 2002)
wild_type
(Satterfield et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Satterfield et al., 2002)
wild_type
(Satterfield et al., 2002)
Mutagen
in vitro construct
(Satterfield et al., 2002)
Progenitor genotype
Carried in construct
P{UAS-Atx2.S}
Cytology
Description

UASt regulatory sequences drive expression of a full length Atx2 cDNA (EST GH27029).

(Satterfield et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Atx2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing Atx2Scer\UAS.cSa in the developing eye under the control of Scer\GAL4GMR.PF have a mildly rough external eye surface, but exhibit no obvious degeneration externally or internally.

      Expression of Atx2Scer\UAS.cSa in differentiated photoreceptor neurons under the control of Scer\GAL4ninaE.PD are born with the normal complement of seven visible photoreceptors per ommatidium but progressive degeneration is seen, reducing the number of photoreceptors from 7 per ommatidium to on average 5.9 by 18 days. A stronger insertion of Atx2Scer\UAS.cSa results in mild, but significant, photoreceptor loss even in 1 day old flies, which becomes progressively worse with age, reaching 1.5 photoreceptors per ommatidium by 18 days.

      (Lessing and Bonini, 2008)

      Expression of Atx2Scer\UAS.cSa under the control of Scer\GAL4GMR.PU results in mildly disorganized ommatidia and interommatidial bristles as well as morphological defects in the photoreceptor neurons of adult eye. Expression under the Scer\GAL4sca-109-68 driver does not affect the number of macrochate in adult flies.

      (Al-Ramahi et al., 2007)

      Atx2X1 flies rescued by Atx2Scer\UAS.cSa (in the absence of a Scer\GAL4 driver) are uncoordinated, can have a rough eye phenotype and have bent and forked thoracic sensory bristles. Many of the bristles have highly disorganised cuticular ridges and the actin bundles are shorter and thinner than normal. Expression of Atx2Scer\UAS.cSa under the control of Scer\GAL4GMR.PF results in a rough eye phenotype. Punctate actin aggregates and disorganised actin clusters are seen in the eye discs. Expression of Atx2Scer\UAS.cSa under the control of Scer\GAL4ey.PB results in lethality and a loss of head structures.

      (Satterfield et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Suppressed by
      Statement
      Reference

      Atx2UAS.cSa, Scer\GAL4ninaE.PD has photoreceptor | conditional phenotype, suppressible | partially by pAbpUAS.cSa, Scer\GAL4ninaE.PD

      (Lessing and Bonini, 2008)
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Atx2UAS.cSa, Scer\GAL430Y is a non-suppressor of adult brain phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL430Y

      (Ghosh and Feany, 2004)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      One copy of Df(2R)ED3610 enhances the visible photoreceptor loss seen when Atx2Scer\UAS.cSa is expressed in differentiated photoreceptor neurons under the control of Scer\GAL4ninaE.PD. 1.3 photoreceptors are seen per ommatidium in 6 day old flies, as opposed to the seven seen in wild type. This compares to 1.7 when Atx2Scer\UAS.cSa is expressed alone.

      Expression of pAbpScer\UAS.cSa partially suppresses the visible photoreceptor loss seen when Atx2Scer\UAS.cSa is expressed in differentiated photoreceptor neurons under the control of Scer\GAL4ninaE.PD. On average 5.1 photoreceptors are seen per ommatidium in 18 day old flies, as opposed to the seven seen in wild type. This compares to 2.9 when Atx2Scer\UAS.cSa is expressed alone.

      (Lessing and Bonini, 2008)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of Atx2Scer\UAS.cSa and Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 in the developing eye under the control of Scer\GAL4GMR.PF produces a more severe phenotype than in either mutant alone. The flies exhibit strong eye degeneration with loss of pigmentation and severe collapse of the retina.

      Expression of Hsap\ATXN3fl.Q84.Scer\UAS.T:Hsap\MYC does not enhance the eye phenotypes seen when Atx2Scer\UAS.cSa is expressed in the developing eye under the control of Scer\GAL4GMR.PF.

      Co-expression of Atx2Scer\UAS.cSa and Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 in differentiated photoreceptor neurons under the control of Scer\GAL4ninaE.PD enhances the photoreceptor loss seen when either construct is expressed alone. Flies are still born with the normal complement of seven visible photoreceptors, but whereas at six days the single mutants appear near normal, co-expression results in only 4.7 photoreceptors per ommatidium. At 18 days almost complete photoreceptor loss (1.3 photoreceptors per ommatidium) is seen.

      Co-expression of Atx2Scer\UAS.cSa and Hsap\ATXN3fl.Q84.Scer\UAS.T:Hsap\MYC in differentiated photoreceptor neurons under the control of Scer\GAL4ninaE.PD enhances the visible photoreceptor loss seen when either construct is expressed alone. At 18-23 days only 2.9 photoreceptors are seen per ommatidium, as opposed to the seven seen in wild type. This compares to 6.3 and 6.97 in Atx2Scer\UAS.cSa and Hsap\ATXN3fl.Q84.Scer\UAS.T:Hsap\MYC respectively.

      Expression of Atx2Scer\UAS.cSa enhances the rate of inclusion formation seen when Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 is expressed in differentiated photoreceptor neurons under the control of Scer\GAL4ninaE.PD. Inclusions are more prominent at 24 hours, but by four days appear similar to when Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 is expressed alone.

      (Lessing and Bonini, 2008)

      The defective adult eye morphology (disorganization of ommatidia and loss of bristles, retinal tissue loss, photoreceptor cell defects) observed in flies expressing either Hsap\ATXN182Q.Scer\UAS or Atx2Scer\UAS.cSa under the control of Scer\GAL4GMR.PU are exacerbated further when the two transgenes are co-expressed. The eye defects in Hsap\ATXN182Q.Scer\UAS expressing flies are partially suppressed by combination with a single copy of Atx2X1. This suppression of the eye phenotype can be reverted (and the phenotype worsened again) by combination with Atx2Scer\UAS.cSa.

      The mild loss of macrochate observed in flies expressing Hsap\ATXN182Q.Scer\UAS under the control of Scer\GAL4sca-109-68 is strongly exacerbated by co-expression of Atx2Scer\UAS.cSa.

      (Al-Ramahi et al., 2007)
      Complementation and Rescue Data
      Rescues

      Atx2UAS.cSa rescues Atx206490

      (Satterfield et al., 2002)
      Comments

      P{UAS-Atx2.S} insertions can rescue the lethality of Atx206490 homozygotes in the absence of a Scer\GAL4 driver, presumably due to leaky transgenic expression of Atx2.

      (Satterfield et al., 2002)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Atx2Scer\UAS.cSa
      Atx2UAS.cSa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Satterfield
      (Satterfield et al., 2002)
      Secondary FlyBase IDs
        References (7)