FB2024_03 , released June 25, 2024
Allele: Dmel\crokKG06053a
Open Close
General Information
Symbol
Dmel\crokKG06053a
Species
D. melanogaster
Name
FlyBase ID
FBal0148289
Feature type
allele
Associated gene
Dmel\crok
Associated Insertion(s)
P{SUPor-P}crokKG06053a
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Gene Disruption Project members, 2001-)
Progenitor genotype
Associated Insertion(s)
P{SUPor-P}crokKG06053a
Cytology
Description

Insertion in the second protein coding exon.

(Nilton et al., 2010)
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous and crokKG06053a/Df(2L)esc-P3-0 stage 16 embryos have an elongated and convoluted dorsal trunk compared to wild-type controls. The luminal chitin matrix appears wider than normal and is amorphous in the mutant dorsal trunks. Ultrastructurally, the trachea of mutant stage 16 embryos have reduced and irregular septate junctions and the tracheal cells contain large unusual vesicle-like structures.

      The paracellular diffusion barrier is defective in mutant embryos; 10kDa rhodamine-conjugated dextran injected into the body cavity diffuses rapidly into the tracheal and salivary gland lumens in mutant embryos, in contrast to wild type.

      (Nilton et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      crokKG06053a is rescued by Scer\GAL469B/crokUAS.cNa

      (Nilton et al., 2010)
      Comments

      Expression of crokScer\UAS.cNa under the control of Scer\GAL469B rescues the tracheal defects seen in crokKG06053a embryos.

      (Nilton et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Comments
      Comments

      Excision of the insertion can revert the lethality and the mutant tracheal phenotype.

      (Nilton et al., 2010)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      CG17218KG06053
      (Nilton et al., 2010)
      CG17218KG06053a
      crokKG06053a
      (Tempesta et al., 2017, Deligiannaki et al., 2015)
      crokKG6053
      (Hall et al., 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)