FlyBase Allele Report: Dmel\Rheb[UAS.cSa]

General Information

Symbol

Dmel\Rheb^UAS.cSa

Species

D. melanogaster

Name

UAS construct of Saucedo

FlyBase ID

FBal0152251

Feature type

allele

Associated gene

Dmel\Rheb

Associated Insertion(s)

Carried in Construct

P{UAS-Rheb.S}

Also Known As

UAS-Rheb

Key Links

Transgenic product class

wild_type

(Saucedo et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Saucedo et al., 2003)

Mutagen

in vitro construct

(Saucedo et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Rheb.S}

Cytology

Description

Expression of a XhoI/BglII fragment of a full length Rheb EST (GH10361), is under the control of UASt sequences.

(Saucedo et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Rheb

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle, with Scer\GAL4^Act5C.PP

(Reis and Edgar, 2004)

abnormal neuroanatomy | third instar larval stage

(Wong et al., 2015)

abnormal size | adult stage, with Scer\GAL4^GMR.PF

(Saucedo et al., 2003)

increased cell death, with Scer\GAL4^GMR.PU

(Wang et al., 2009)

increased cell size, with Scer\GAL4^en-e16E

(Saucedo et al., 2003)

increased cell size, with Scer\GAL4^GMR.PF

(Stocker et al., 2003)

increased cell size, with Scer\GAL4^GMR.PU

(Wang et al., 2012)

increased cell size | somatic clone, with Scer\GAL4^Act5C.PP

(Saucedo et al., 2003, Stocker et al., 2003)

visible, with Scer\GAL4^en.PU

(Wang et al., 2012)

visible, with Scer\GAL4^GMR.PU

(Wang et al., 2012)

visible, with Scer\GAL4^ptc-559.1

(Hsu et al., 2007)

Phenotype Manifest In

1st posterior cell, with Scer\GAL4^ptc-559.1

(Hsu et al., 2007)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav-C155

(Wong et al., 2015)

eye, with Scer\GAL4^GMR.PF

(Saucedo et al., 2003)

eye, with Scer\GAL4^GMR.PU

(Wang et al., 2012)

fat body | conditional | somatic clone, with Scer\GAL4^GMR.PF

(Stocker et al., 2003)

mitotic cell cycle, with Scer\GAL4^Act5C.PP

(Reis and Edgar, 2004)

NMJ bouton | third instar larval stage, with Scer\GAL4^elav-C155

(Wong et al., 2015)

nucleolus, with Scer\GAL4^en-e16E

(Grewal et al., 2007)

ommatidium, with Scer\GAL4^GMR.PU

(Wang et al., 2009)

photoreceptor neuron, with Scer\GAL4^GMR.PF

(Stocker et al., 2003)

photoreceptor neuron, with Scer\GAL4^GMR.PU

(Wang et al., 2009)

rhabdomere, with Scer\GAL4^GMR.PU

(Wang et al., 2009)

salivary gland | conditional | somatic clone, with Scer\GAL4^GMR.PF

(Stocker et al., 2003)

wing blade posterior compartment, with Scer\GAL4^en.PU

(Wang et al., 2012)

Detailed Description

Statement

Reference

Ectopic expression of Rheb^Scer\UAS.cSa under the Scer\GAL4^elav-C155 driver increases the number of neuromuscular junction boutons in third instar larvae compared to controls.

(Wong et al., 2015)

Expression of Rheb^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PU greatly increases cell size in the compound eyes of adult flies.

Expression of Rheb^Scer\UAS.cSa under the control of Scer\GAL4^en.PU results in an increase in the size of the posterior compartment of the wing relative to the anterior compartment.

(Wang et al., 2012)

Young flies expressing Rheb^Scer\UAS.cSa in the eye under the control of Scer\GAL4^GMR.PU exhibit normal eye morphology, although the ommatidium size is much larger than in controls. However, these flies undergo an age-dependent loss of photoreceptor cells when cultured on a 12-h light/12-h dark cycle. Flies cultured in continuous darkness lose photoreceptor cells more slowly than those exposed to light, indicating that photoreceptor degeneration in Rheb^Scer\UAS.cSa-overexpressing eyes is dependent on phototransduction.

The eyes of Scer\GAL4^GMR.PF driven Rheb^Scer\UAS.cSa flies are similar in size to controls and have normal retinal morphology when the flies are young. However, few R1-R6 rhabdomeres are detected in light-exposed 30-day old flies, although R7 rhabdomeres are generally present.

Flies expressing Rheb^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF lose major photoreceptor neurons after 30 days of light/dark culture.

(Wang et al., 2009)

Expression of Rheb^Scer\UAS.cSa under the control of Scer\GAL4^en-e16E increases nucleolar size, which is an index of ribosome biogenesis.

(Grewal et al., 2007)

Expression of Rheb^Scer\UAS.cSa under the control of Scer\GAL4^ptc-559.1 results in a 15% increased in the distance between wing veins L3 and L4 compared to controls.

(Hsu et al., 2007)

Rheb^Scer\UAS.cSa; Scer\GAL4^GMR.PF flies have highly enlarged eyes. The wings of Rheb^Scer\UAS.cSa; Scer\GAL4^en-e16E flies have an enlarged posterior compartment (22% expansion; P < 0.0002), with minimal disruption of patterning. Wing hair density in the posterior compartment is decreased to 0.74 of wild-type, corresponding to a 34% increase in adult wing cell area (P > 0.0004). Analysis of cell size by forward scatter analysis of dissociated wing disc cells of the genotype Rheb^Scer\UAS.cSa; Scer\GAL4^Act5C.PP, shows an increase of up to 65% in mean FSC compared to wild-type. The fraction of these cells in G1 is reduced from 43% to 16% compared to wild-type. The doubling time of these cells is only slightly reduced compared to wild-type (13.7 hours vs 13.9 hours for controls). (All of the above data comes from Scer\GAL4^Act5C.PP clones induced 88 hours after egg laying, and harvested/analysed as third instar larvae). In Rheb^Scer\UAS.cSa animals, somatic clones of Scer\GAL4^Act5C.PP cells in the gut, proventriculus and fat body. Such clones induced in fat body prior to endoreplication encompass approximately 2.5 times the area of control cells (P < 0.02) and contain, on average, 64% more DNA (P<0.007) when assayed in wandering, fed L3 larvae. In second instar larvae starved for 72 hours, these clone cells continue to grow, whereas surrounding wild-type cells do not.

(Saucedo et al., 2003)

Rheb^Scer\UAS.cSa ; Scer\GAL4^Act5C.PP clones in the wing imaginal disc attain a significantly larger size than surrounding wild-type cells. This enlargement is caused by a significant increase in cell size (a 48% increase in area covered per cell). Observation of mosaic ommatidia shows that this phenotype is cell autonomous. In contrast, the cell doubling time remains unchanged in these cells compared to control cells (10.5 h versus 10.8 h). The increase in clonal area 40 hours after induction is caused by larger cells (n = 20, P = 0.00002), whereas the number of cells is not significantly altered (P = 0.087). Rheb^Scer\UAS.cSa ; Scer\GAL4^GMR.PF animals have enlarged but fully differentiated photoreceptor cells (rhabdomere size is increased by 66%). Under normal nutrient availability, Rheb^Scer\UAS.cSa ; Scer\GAL4^Act5C.PP clone cells in the salivary gland and fat body display only a mild size increase. In larvae starved for amino acids, Rheb^Scer\UAS.cSa ; Scer\GAL4^Act5C.PP clone cells in the salivary glands display a several fold increase in size and contain much more DNA than non-expressing neighbouring cells, but they do not reach normal size and ploidy. However, Rheb^Scer\UAS.cSa ; Scer\GAL4^Act5C.PP clone cells in the fat body cells of these larvae display a complete reversion the starvation phenotype: the size, DNA content, and appearance (amount of vesicles) of these cells are indistinguishable from non-starved cells.

(Stocker et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rheb^UAS.cSa has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by wnd[+]/wnd³

(Wong et al., 2015)

Rheb^UAS.cSa, Scer\GAL4^GMR.PU has increased cell death phenotype, suppressible by Atg1^{ninaE.Tag:MYC}

(Wang et al., 2009)

Rheb^UAS.cSa, Scer\GAL4^Act5C.PP has abnormal mitotic cell cycle phenotype, suppressible by Dp^a1/Dp^a2

(Reis and Edgar, 2004)

NOT suppressed by

Statement

Reference

Rheb^UAS.cSa, Scer\GAL4^GMR.PU has visible phenotype, non-suppressible by Lst8¹

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^en.PU has visible phenotype, non-suppressible by Lst8¹

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has visible phenotype, non-suppressible by Lst8^UAS.cWa, Scer\GAL4^GMR.PU

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has visible phenotype, non-suppressible by Lst8¹

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^en.PU, mTor^UAS.cWa, raptor^UAS.cWa has visible phenotype, non-suppressible by Lst8^UAS.cWa, Scer\GAL4^en.PU

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^en.PU, mTor^UAS.cWa, raptor^UAS.cWa has visible phenotype, non-suppressible by Lst8¹

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^GMR.PU has increased cell size phenotype, non-suppressible by Lst8¹

(Wang et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^arm.PU/Rheb^UAS.cSa is a non-suppressor of decreased body size | third instar larval stage phenotype of Tcs3^e01173

(Rojas-Benitez et al., 2015)

Scer\GAL4^αTub84B.PL/Rheb^UAS.cSa is a non-suppressor of abnormal mitotic cell cycle | somatic clone phenotype of mTor^ΔP

(Saucedo et al., 2003)

Scer\GAL4^αTub84B.PL/Rheb^UAS.cSa is a non-suppressor of decreased cell size | somatic clone phenotype of mTor^ΔP

(Saucedo et al., 2003)

Other

Statement

Reference

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has visible phenotype

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^en.PU, mTor^UAS.cWa, raptor^UAS.cWa has visible phenotype

(Wang et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rheb^UAS.cSa, Scer\GAL4^elav-C155 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by wnd[+]/wnd³

(Wong et al., 2015)

Rheb^UAS.cSa, Scer\GAL4^elav-C155 has NMJ bouton | third instar larval stage phenotype, suppressible by wnd[+]/wnd³

(Wong et al., 2015)

Rheb^UAS.cSa, Scer\GAL4^Act5C.PP has mitotic cell cycle phenotype, suppressible by Dp^a1/Dp^a2

(Reis and Edgar, 2004)

Rheb^UAS.cSa, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by S6k^l-1/S6k^l-1

(Saucedo et al., 2003)

NOT suppressed by

Statement

Reference

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has eye phenotype, non-suppressible by Lst8^UAS.cWa, Scer\GAL4^GMR.PU

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has eye phenotype, non-suppressible by Lst8¹

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has ommatidium phenotype, non-suppressible by Lst8^UAS.cWa, Scer\GAL4^GMR.PU

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has ommatidium phenotype, non-suppressible by Lst8¹

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by Lst8¹

(Wang et al., 2012)

Suppressor of

Statement

Reference

Rheb^UAS.cSa, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF, Tsc1^UAS.cPa, gig^UAS.cTa

(Saucedo et al., 2003)

NOT Suppressor of

Statement

Reference

raptor^UAS.cWa, mTor^UAS.cWa, Rheb^UAS.cSa, Scer\GAL4^en.PU is a non-suppressor of wing blade posterior compartment phenotype of Lst8^UAS.cWa, Scer\GAL4^en.PU

(Wang et al., 2012)

Scer\GAL4^en.PU/Rheb^UAS.cSa is a non-suppressor of wing blade posterior compartment phenotype of Lst8¹

(Wang et al., 2012)

Scer\GAL4^en.PU, raptor^UAS.cWa, mTor^UAS.cWa, Rheb^UAS.cSa is a non-suppressor of wing blade posterior compartment phenotype of Lst8¹

(Wang et al., 2012)

Other

Statement

Reference

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has eye phenotype

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has ommatidium phenotype

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^en.PU, mTor^UAS.cWa, raptor^UAS.cWa has wing blade posterior compartment phenotype

(Wang et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of neuromuscular junction boutons in third instar larvae expressing Rheb^Scer\UAS.cSa under the Scer\GAL4^elav-C155 driver is significantly suppressed by combination with wnd³/+.

(Wong et al., 2015)

Lst8¹ does not suppress the increased cell size seen in the eyes of flies expressing Rheb^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PU.

Lst8¹ des not suppress the overgrowth of the posterior compartment of the wing relative to the anterior compartment which is seen in flies expressing Rheb^Scer\UAS.cSa under the control of Scer\GAL4^en.PU.

Flies co-expressing Tor^Scer\UAS.cWa, raptor^Scer\UAS.cWa and Rheb^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PU completely lack photoreceptor cells in the eye. Evidence of cell death, such as accumulation of prominent vacuoles and highly condensed particles, is seen. This phenotype is still seen if the flies are either also co-expressing Lst8^Scer\UAS.cWa or are mutant for Lst8¹.

Flies co-expressing Tor^Scer\UAS.cWa, raptor^Scer\UAS.cWa and Rheb^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PU (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit expression to 4 hours immediately following pupa formation) show a dramatic increase in ommatidial size compared to controls. This phenotype is still seen if the flies are either also co-expressing Lst8^Scer\UAS.cWa or are mutant for Lst8¹.

Flies co-expressing Tor^Scer\UAS.cWa, raptor^Scer\UAS.cWa and Rheb^Scer\UAS.cSa under the control of Scer\GAL4^en.PU (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit expression to 4 hours immediately following pupa formation) show overgrowth of the posterior compartment of the wing relative to the anterior compartment. This phenotype is still seen if the flies are either also co-expressing Lst8^Scer\UAS.cWa or are mutant for Lst8¹.

(Wang et al., 2012)

Co-expression of Atg1^{ninaE.T:Hsap\MYC} with Scer\GAL4^GMR.PF Rheb^Scer\UAS.cSa suppresses the neuronal degeneration found upon expression of Rheb^Scer\UAS.cSa alone.

(Wang et al., 2009)

The distance between wing veins L3 and L4 is smaller than normal in flies co-expressing Rheb^Scer\UAS.cSa and Tctp^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ptc-559.1, resembling the phenotype seen in flies expressing Tctp^{dsRNA.Scer\UAS} alone under the control of Scer\GAL4^ptc-559.1.

(Hsu et al., 2007)

The compensatory elongation of G2 phase seen in cells expressing Rheb^Scer\UAS.cSa driven by Scer\GAL4^Act5C.PP is not as pronounced in a Dp^a1/Dp^a2 background.

Cells expressing Rheb^Scer\UAS.cSa driven by Scer\GAL4^Act5C.PP in a Dp^a1/Dp^a2 background, the compensatory elongation of the G2 phase, is not as pronounced seen in dm^Scer\UAS.cZa expressing cells.

(Reis and Edgar, 2004)

Eye overgrowth in Rheb^Scer\UAS.cSa; Scer\GAL4^GMR.PF animals is not suppressed by S6k^l-1/S6k^l-1, but ommatidia are smaller and less disorganized than those of Rheb^Scer\UAS.cSa; Scer\GAL4^GMR.PF alone, and the resulting eyes have a distinctive puckered appearance. The small size and increase in proportion of cells in G1 seen in cell from Tor^ΔP homozygous wing disc clones, is unafected by the expression of Rheb^Scer\UAS.cSa in the clone cells (driven by Scer\GAL4^αTub84B.PL when Scer\GAL80^αTub84B.PL is removed in the same mitotic recombination event that creates the Tor^ΔP homozygous clone.)

(Saucedo et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^hs.PB/Rheb^UAS.cSa partially rescues Rheb^PΔ2/Rheb^PΔ1

(Saucedo et al., 2003)

Comments

With or without heat-shock, Rheb^Scer\UAS.cSa; Scer\GAL4^hs.PB partially rescues the growth defect of Rheb^PΔ1/Rheb^PΔ2 animals, enabling them to reach the second larval stage before arresting.

(Saucedo et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

80932

w^*; P{UAS-Rheb.S}13.1; P{UAS-mTor.WT}III

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Rheb^Scer\UAS.cSa

Rheb^UAS.cSa

Name Synonyms

UAS construct of Saucedo

(Saucedo et al., 2003)

Secondary FlyBase IDs

References (16)

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