FB2024_03 , released June 25, 2024
Allele: Dmel\mbf12
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General Information
Symbol
Dmel\mbf12
Species
D. melanogaster
Name
FlyBase ID
FBal0155019
Feature type
allele
Associated gene
Dmel\mbf1
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Jindra et al., 2004, Liu et al., 2003)
Progenitor genotype
Cytology
Description

Imprecise excision of a P{lacW} element inserted just upstream of mbf1, resulting in a 2082bp deletion of genomic sequence extending from the original insertion site into the mbf1 coding region.

(Jindra et al., 2004)

Imprecise excision of a P-element has deleted from 211 bp upstream of the initiation codon of mbf1 to 857 bp downstream of the stop codon.

(Liu et al., 2003)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:16,574,643..16,576,726 [+]
Comment:

Excision deletion location based on report that deletion extends from 211bp upstream of AUG to 857bp downstream of stop codon and has an extent of 2082bp.

(Jindra et al., 2004, Liu et al., 2003)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      mbf12 heterozygous or homozygous males do not show ectopic sex comb teeth on the second leg.

      (Nishioka et al., 2018)

      Homozygous first instar larvae fed on a diet containing 0.1 or 0.3% hydrogen peroxide reach adulthood about 3.5 times less frequently than controls. Homozygous adult males (derived from homozygous females mated to homozygous males) exposed to 0.5% hydrogen peroxide have a median survival time of 67 hours, compared to 93 hours for controls. mbf12/Df(3L)st7P hemizygous adults derived from Df(3L)st7P/+ females mated to mbf12/+ males live on average 64 hours on 0.5% hydrogen peroxide, indicating that there is no maternal effect. into 'turbans', and the peduncle and lobes are absent in most flies.

      (Jindra et al., 2004)

      mbf12 homozygous animals derived from mbf12 heterozygous parents are completely viable. However breakages in CNS and tracheal networks are seen in these embryos at a very low penetrance (2-3%). A variable fraction of progeny from mbf12 homozygous females die before stage 5, regardless of whether the paternal genotype is mbf12 or mbf1 wild-type. Embryos that develop have no segmentation defects and develop to adults with no obvious morphological defects.

      (Liu et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Other
      Statement
      Reference

      JraIA109, mbf12 has visible phenotype

      (Jindra et al., 2004)

      kay1, mbf12 has visible phenotype

      (Jindra et al., 2004)
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Statement
      Reference

      Psc1, mbf1[+]/mbf12 has posterior wing margin | male phenotype, suppressible by pcmΔ1

      (Nishioka et al., 2018)
      Enhancer of
      Statement
      Reference

      mbf1[+]/mbf12 is an enhancer of sex comb tooth | ectopic phenotype of Psc1

      (Nishioka et al., 2018)

      pcmhs.PG, mbf1[+], mbf12, pcmΔ1/pcmΔ1 is an enhancer of sex comb tooth | ectopic phenotype of Psc1

      (Nishioka et al., 2018)

      mbf1[+]/mbf12 is an enhancer of sex comb tooth | ectopic phenotype of Pc6

      (Nishioka et al., 2018)

      pcmhs.PG, mbf1[+], mbf12, pcmΔ1/pcmΔ1 is an enhancer of sex comb tooth | ectopic phenotype of Pc6

      (Nishioka et al., 2018)
      NOT Enhancer of
      Statement
      Reference

      mbf1[+], mbf12, mbf1+t4574 is a non-enhancer of sex comb tooth | ectopic phenotype of Psc1

      (Nishioka et al., 2018)

      mbf1[+], mbf12, mbf1+t4574 is a non-enhancer of sex comb tooth | ectopic phenotype of Pc6

      (Nishioka et al., 2018)
      NOT Suppressor of
      Statement
      Reference

      mbf1[+]/mbf12, pcmΔ1/pcmΔ1 is a non-suppressor of sex comb tooth | ectopic phenotype of Pc6

      (Nishioka et al., 2018)

      mbf1[+]/mbf12, pcmΔ1/pcmΔ1 is a non-suppressor of sex comb tooth | ectopic phenotype of Psc1

      (Nishioka et al., 2018)

      mbf1[+]/mbf12, pcm5/pcm5 is a non-suppressor of sex comb tooth | ectopic phenotype of Psc1

      (Nishioka et al., 2018)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The heat-shock-induced expression of pcmhs.PG in mbf12 heterozygotes leads to a low penetrance ectopic sex comb teeth phenotype, as compared to controls.

      Psc1, mbf12 double heterozygotes show adult wing defects along the posterior wing margin in males, which are suppressed by pcmΔ1 hemizygosity.

      The ectopic sex comb teeth phenotype of Psc1 heterozygous males is partially suppressed by homozygosity for either pcmΔ1 or pcm5 and is incrementally exacerbated by heterozygosity and homozygosity for mbf12; the phenotype suppression by either pcmΔ1 or pcm5 homozygosity is annulled by heterozygosity for mbf12; the phenotype enhancement induced by mbf12 heterozygosity is annulled by one copy of mbf1+t4574. The ectopic sex comb teeth phenotype of Psc1 heterozygous males is also exacerbated by the triple combinations of heterozygosity for mbf12, homozygosity for either pcmΔ1 or pcm5 and heat-shock-induced expression of pcmhs.PG.

      Similarly, the ectopic sex comb teeth phenotype of Pc6 heterozygous males is partially suppressed by homozygosity for pcmΔ1 and is exacerbated by heterozygosity for mbf12; the phenotype enhancement by mbf12 heterozygosity is annulled by one copy of mbf1+t4574; the phenotype suppression by pcmΔ1 homozygosity is annulled by heterozygosity for mbf12. The ectopic sex comb teeth phenotype of Pc6 heterozygous males is also exacerbated by the triple combination of mbf12 heterozygosity, pcmΔ1 homozygosity and heat-shock-induced expression of pcmhs.PG.

      (Nishioka et al., 2018)

      JraIA109/+ ; mbf12 double mutant adults treated with hydrogen peroxide at the onset of metamorphosis often have cuticle defects, which manifest as a depressed patch of naked cuticle at the dorsal midline. mbf12 kay1/mbf12 double mutant adults treated with hydrogen peroxide at the onset of metamorphosis often have cuticle defects, which manifest as a depressed patch of naked cuticle at the dorsal midline.

      (Jindra et al., 2004)

      The penetrance of breakages in CNS and tracheal networks of mbf12 homozygous embryos is dominantly enhanced by apttdf-PΔ3.

      (Liu et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      mbf12 is rescued by mbf1+t4574

      (Jindra et al., 2004)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      mbf1-
      (Liu et al., 2003)
      mbf12
      (Nishioka et al., 2018)
      mbf1Δ1
      Name Synonyms
      Secondary FlyBase IDs
        References (3)