FlyBase Allele Report: Dmel\pirk[EY00723]

General Information

Symbol

Dmel\pirk^EY00723

Species

D. melanogaster

Name

FlyBase ID

FBal0157158

Feature type

allele

Associated gene

Dmel\pirk

Associated Insertion(s)

P{EPgy2}pirk^EY00723

Carried in Construct

Also Known As

pirk^ey

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EPgy2}pirk^EY00723

Cytology

Description

Insertion 62bp upstream of the translational start site.

(Lhocine et al., 2008)

Allele components

Component

Use(s)

Inserted element

P{EPgy2}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:21,661,979..21,661,979 [+]

(Paredes et al., 2011, Gendrin et al., 2009, Aggarwal et al., 2008, Lhocine et al., 2008, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response

(Aggarwal et al., 2008)

abnormal immune response, with Scer\GAL4^da.G32

(Gendrin et al., 2009)

fertile

(Lhocine et al., 2008)

short lived

(Lhocine et al., 2008)

short lived | conditional

(Paredes et al., 2011)

viable

(Lhocine et al., 2008)

Phenotype Manifest In

Detailed Description

Statement

Reference

pirk^EY00723/pirk^EY00723 mutants do not exhibit a significant change in survival in response to P. rettgeri or L. monocytogenes infection, as compared to wild type.

(Morris et al., 2016)

pirk^EY00723 flies infected with E.carotovora show reductions in mean lifespan compared to wild-type.

(Paredes et al., 2011)

pirk^EY00723 mutant flies display a higher than wild-type (2.5 fold) immune response to genital infection with E.carotovora.

(Gendrin et al., 2009)

pirk^EY00723 mutant flies infected with the gram-negative pathogen Erwinia carotovora carotovora show significantly improved survival compared to wild-type flies.

(Aggarwal et al., 2008)

pirk[EY00723] homozygous mutant animals are viable and fertile but display a marked reduction of their life span, as compared to controls.

(Lhocine et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Df(2R)PGRP-SC^Δ, PGRP-LB^Δ, pirk^EY00723 has increased occurrence of cell division | adult stage phenotype

(Paredes et al., 2011)

PGRP-LB^Δ/Df(2R)PGRP-SC^Δ, Dredd¹, pirk^EY00723 has long lived phenotype

(Paredes et al., 2011)

PGRP-LB^Δ/Df(2R)PGRP-SC^Δ, Rel^E20, pirk^EY00723 has long lived phenotype

(Paredes et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ and pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ, Df(3L)PGRP-SB^Δ5 flies are viable, although not fully fertile.

After oral infection with E. carotovora, the addition of pirk^EY00723 to PGRP-LB^Δ; Df(3L)PGRP-SC1^Δ results in an enhancement of the immune response locally and systemically. As with septic injury, pirk^EY00723, PGRP-LB^Δ; Df(3L)PGRP-SC1^Δ flies being to day within 4 days of oral infection with E. carotovora.

pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ flies begin to die within 4 days of oral infection with E. carotovora. Although pirk^EY00723 Df(2R)PGRP-SC^Δ flies show the same level of response as pirk^EY00723 flies, it is important to note that the response of pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ flies is significantly higher than that of pirk^EY00723 Df(2R)PGRP-SC^Δ flies.

The stronger immune responses to E. carotovora oral infection of Df(2R)PGRP-SC^Δ PGRP-LB^Δ flies is correlated with a further decrease in lifespan compared to single mutant flies. pirk^EY00723 Df(2R)PGRP-SC^Δ; PGRP-LB^Δ mutant flies do not simply show an incremental reduction in their lifespan, but rather die rapidly with oral E. carotovora infection, decreasing 50% after only 5 days. These flies are also susceptible to oral infection with dead sonicated E. carotovora, demonstrating that is it not bacteria that are killing the fly but rather its own excessive immune response.

Dredd¹; pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ and pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ, Rel^E20 flies, with impaired imd pathway activity due to the presence of Dredd or Rel mutations, exhibit an increased lifespan upon oral infection with E. carotovora bacteria, compared to pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ flies.

pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ and Df(2R)PGRP-SC^Δ; PGRP-LB^Δ flies in unchallenged conditions exhibit a marked reduction in lifespan.

The level of epithelium renewal, as evidenced by the number of mitotic cells along the midgut, is very high in pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ flies in the absence of bacterial infection, approaching the level seen in infected wild-type guts. The mitotic index of these flies doubles between unchallenged to E. carotovora oral infection conditions, suggesting that the level of epithelium renewal in the triple mutant is approaching the limit of cells available to undergo mitosis.

(Paredes et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG15678^EY00723

Pirk^EY00723

(Neyen et al., 2016)

pirk^EY00723

(Maitra et al., 2019, Khadilkar et al., 2017, Kurz et al., 2017, Tavignot et al., 2017, Bosco-Drayon et al., 2012)

pirk^ey

(Morris et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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