FlyBase Allele Report: Dmel\topi[Z3-0707]

General Information

Symbol

Dmel\topi^Z3-0707

Species

D. melanogaster

Name

FlyBase ID

FBal0158556

Feature type

allele

Associated gene

Dmel\topi

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Perezgasga et al., 2004)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q261term.

(Perezgasga et al., 2004)

Nucleotide substitution: C784T.

(Perezgasga et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:9,776,126..9,776,126 [+]

Nucleotide change:

C9776126T

Reported nucleotide change:

C784T

Amino acid change:

Q262term | topi-PA

Reported amino acid change:

Q261term

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement.

(Perezgasga et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | adult stage (with topi^Z3-2139)

(Lu and Fuller, 2015)

abnormal meiotic cell cycle | male

(Perezgasga et al., 2004)

female fertile

(Perezgasga et al., 2004)

male sterile

(Perezgasga et al., 2004)

viable

(Perezgasga et al., 2004)

Phenotype Manifest In

nuclear lamina | adult stage | male (with topi^Z3-2139)

(Trost et al., 2016)

nucleolus | adult stage (with topi^Z3-2139)

(Lu and Fuller, 2015)

secondary spermatocyte

(Perezgasga et al., 2004)

spermatid

(Perezgasga et al., 2004)

spermatocyte | adult stage (with topi^Z3-2139)

(Lu and Fuller, 2015)

Detailed Description

Statement

Reference

topi^Z3-2139/topi^Z3-0707 heterozygote males show morphological defects of the nuclear lamina in stage S4-S6 spermatocytes (the lamina has more ballon-like shape, largely lacking the deformations that correlate with chromosome distribution seen in wild-type), while in stage S3 the lamina is of wild-type appearance.

(Trost et al., 2016)

topi^Z3-2139/topi^Z3-0707 mutant spermatocytes exhibit meiotic arrest and other defects, including bivalent chromosomes condensing to spherical shapes away from the nuclear periphery, and failure of the nucleolus to fully break down.

(Lu and Fuller, 2015)

The testes of topi^Z3-0707/topi^Z3-0707 adult males contain germ cell cysts up to and including mature primary spermatocytes, but lack germ-cells in meiotic division or spermatid differentiation stages. Degenerating primary spermatocytes are present in the distal region of the testis.

(Perezgasga et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

38441

bw¹; topi^Z3-0707/TM6B, Tb¹

Notes on Origin

Discoverer

Zuker

(Perezgasga et al., 2004)

From C.Zucker's collection of viable, male sterile lines.

(Perezgasga et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

topi^Z0707

(Lu and Fuller, 2015)

topi^Z3-0707

(Trost et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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