FlyBase Allele Report: Dmel\loqs[f00791]

(Fukunaga et al., 2012, Liu et al., 2012, Han et al., 2011, Marques et al., 2010, Reich et al., 2009, Forstemann et al., 2005, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen et al., 2006)

modeled by Hsap\ATXN3^{fl.Q84.UAS.Tag:MYC}

(Bilen et al., 2006)

modeled by Hsap\MAPT^UAS.cWa

(Bilen et al., 2006)

modeled by Hsap\MAPT^R406W.UAS

(Bilen et al., 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

loqs^f00791 mutant flies have a significantly shortened lifespan and show late-onset brain morphological deterioration. The brains of young adults appear normal, but by 25 days the flies have developed large vacuoles in the retina and lamina of the brain.

(Liu et al., 2012)

loqs^f00791 mutants display a reduction in dsRNA processing and a subsequent ~60% reduction in siRNA levels.

(Marques et al., 2010)

The longitudinal and commissural axon structures of the central nervous system are disrupted in homozygous embryos.

Approximately 70% of homozygotes die before adulthood, and surviving males have reduced fertility and surviving females are sterile. Surviving adults are often stuck in the food.

Homozygous adults have significantly reduced locomotor activity compared to controls.

(Bennett et al., 2008)

loqs^f00791/loqs^KO females have an average of 0.5 germline stem cells (GSCs) per germarium at 10 days after eclosion, compared to an average of 2-3 GSCs per germarium in wild-type females at this age. Differentiated cystoblasts with posterior-positioned fusomes are often seen at the normal location of GSCs in the germaria of loqs^f00791/loqs^KO females.

(Park et al., 2007)

loqs^f00791 mutants exhibit minimal disruption to the eye.

(Bilen et al., 2006)

Only 17% of embryos derived from homozygous males mated to wild-type females hatch. Mutant males contain crystals of Ste protein in their testes. Mutant females have small ovaries and are completely sterile, failing to lay any eggs. The mutant ovarioles contain a smaller than normal germarium and contain only 2 or 3 previtellogenic egg chambers and a late-stage egg chamber (in contrast to the developmentally ordered array of 6-8 egg chambers seen in wild-type ovarioles). The mutant germarium contains only a few germ-line cells, which are not organised into distinct cysts. Spectrosomes can not be detected in the mutant germaria of 3-4 day old females, suggesting that no stem cells remain in these flies. The follicle cell layer is significantly reduced compared to wild-type. Mature oocytes in the mutant ovarioles have normal dorsal appendages.

(Forstemann et al., 2005)

loqs^f00791 mutant females are completely sterile while males are approximately 60-70% sterile when compared with the control crosses between heterozygous and wild-type flies. Although mutant testes appear normal, mutant ovaries contain a few maturing egg chambers and a shriveled germarium with few healthy germline stem cells. The mutant ovary does not sustain continuous egg chamber production since germline stem cells can not be properly maintained.

(Jiang et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

loqs[+]/loqs^f00791 is an enhancer of partially lethal - majority die | male phenotype of Df(1)roX2Δ, lncRNA:roX1^ex33A

(Menon and Meller, 2012)

loqs^f00791, Scer\GAL4^GMR.PF is an enhancer of increased cell death phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

loqs^f00791 is an enhancer of increased cell death phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

loqs^f00791, Scer\GAL4^GMR.PF is an enhancer of increased cell death phenotype of Hsap\ATXN3^{fl.Q84.UAS.Tag:MYC}, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

loqs^f00791, Scer\GAL4^GMR.PF is an enhancer of increased cell death phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

Suppressor of

Statement

Reference

loqs^f00791/loqs^f00791 is a suppressor of abnormal eye color phenotype of w^RNAi.GMR

(Forstemann et al., 2005)

Other

Statement

Reference

Df(1)roX2Δ, lncRNA:roX1^ex40A, loqs^f00791 has partially lethal - majority die | male phenotype

(Menon and Meller, 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

loqs^f00791, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

loqs^f00791, Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

loqs^f00791, Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

loqs^f00791, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\ATXN3^{fl.Q84.UAS.Tag:MYC}, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

loqs^f00791, Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Hsap\ATXN3^{fl.Q84.UAS.Tag:MYC}, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

loqs^f00791, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

loqs^f00791, Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

loqs^f00791, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

Suppressor of

Statement

Reference

loqs^f00791 is a suppressor of eye phenotype of w^RNAi.GMR

(Marques et al., 2010)

loqs^f00791/r2d2¹ is a suppressor of eye phenotype of w^RNAi.GMR

(Marques et al., 2010)

loqs[+]/loqs^f00791 is a suppressor of male germline cell phenotype of mael^M391/Df(3L)79E-F

(Pek et al., 2009)

loqs^f00791/loqs^f00791 is a suppressor of pigment cell phenotype of w^RNAi.GMR

(Forstemann et al., 2005)

NOT Suppressor of

Statement

Reference

loqs^f00791/loqs^KO is a non-suppressor of female germline cell phenotype of bam^unspecified

(Park et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The survival of roX1^ex33A Df(1)roX2Δ adult males is reduced if they are also heterozygous for loqs^f00791.

roX1^ex40A Df(1)roX2Δ ; loqs^f00791/loqs^f00791 males show less than 50% survival.

(Menon and Meller, 2012)

loqs^f00791 mutant flies carrying two copies of w^IR.GMR exhibit impaired w silencing and display orange-red eyes.

loqs^f00791 r2d2¹ double mutant flies carrying two copies of w^IR.GMR exhibit impaired w silencing and display orange-red eyes.

loqs^f00791 r2d2¹ double mutants display a reduction in dsRNA processing and a subsequent ~80% reduction in siRNA levels.

(Marques et al., 2010)

The differentiation defects seen in the testes of mael^M391/Df(3L)79E-F males are partially rescued by loqs^f00791/+.

(Pek et al., 2009)

The germ cell tumour phenotype seen in bam^unspecified females is not suppressed by loqs^f00791/loqs^KO. The double mutant germaria retain germline stem cells.

(Park et al., 2007)

Eye degeneration due to expression of Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} in the eye (under the control of Scer\GAL4^GMR.PF) is enhanced in a loqs^f00791 background, resulting in a severely degenerated eye with complete loss of pigmentation.

Eye degeneration due to expression of Hsap\MJD^{fl.Q84.Scer\UAS.T:Hsap\MYC} in the eye (under the control of Scer\GAL4^GMR.PF) is enhanced in a loqs^f00791 background, resulting in dramatically reduced retinal thickness.

Eye degeneration due to expression of Hsap\MAPT^Scer\UAS.cWa in the eye (under the control of Scer\GAL4^GMR.PF) is enhanced in a loqs^f00791 background.

Eye degeneration due to expression of Hsap\MAPT^{R406W.Scer\UAS} in the eye (under the control of Scer\GAL4^GMR.PF) is enhanced in a loqs^f00791 background.

(Bilen et al., 2006)

The silencing of the w gene seen in flies carrying P{GMR-wIR} is less efficient if the flies are also homozygous for loqs^f00791; flies carrying two copies of P{GMR-wIR} and homozygous for loqs^f00791 do not have completely white eyes (in contrast to flies carrying two copies of P{GMR-wIR} in a wild-type background).

(Forstemann et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

loqs^f00791/loqs^KO is rescued by loqs^PB.Tag:MYC

(Park et al., 2007)

loqs^f00791/loqs^KO is rescued by Scer\GAL4^{VP16.nanos.UTR}/loqs^{PB.UASp.Tag:MYC}

(Park et al., 2007)

Not rescued by

loqs^f00791/loqs^KO is not rescued by loqs^PA.Tag:MYC

(Park et al., 2007)

loqs^f00791/loqs^KO is not rescued by loqs^{PB.UASp.Tag:MYC}/Scer\GAL4^bab1-Agal4-5

(Park et al., 2007)

loqs^f00791/loqs^KO is not rescued by Scer\GAL4^C587/loqs^{PB.UASp.Tag:MYC}

(Park et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

18371

w¹¹¹⁸; PBac{WH}loqs^f00791/CyO

Kyoto

114651

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; PBac{WH}loqs^f00791 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

Separable from: a second mutation on the chromosome. The loqs^f00791 mutation does not cosegregate with the female sterility of the "f00791" chromosome.

(Park et al., 2007)

Comments

Precise excision of the insertion reverts the female sterile phenotype.

(Forstemann et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CG6866^f00791

(Gene Disruption Project members and Exelixis, 2005)

R3D1^f00791

(Bilen et al., 2006)

dRax^f00791

(Bennett et al., 2008)

loqs⁰⁰⁷⁹¹

(Vagin et al., 2006)

loqs^f00791

(Eyk et al., 2019, Fukunaga et al., 2012, Liu et al., 2012, Menon and Meller, 2012, Dufourt et al., 2011, Han et al., 2011, Liu et al., 2011, Marques et al., 2010, Pek et al., 2009, Reich et al., 2009, Zhou et al., 2009, Czech et al., 2008, Park et al., 2007, Park et al., 2007, Yang et al., 2007)

r3d1^PB

(Jiang et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (24)

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