FlyBase Allele Report: Dmel\ds[UAS.cTa]

Expressing ds^Scer\UAS.cTa under the control of Scer\GAL4^hh.PU results in smaller relative posterior wing compartment area, as compared to wild-type controls.

Expressing ds^Scer\UAS.cTa under the controls of Scer\GAL4^en.PU results in smaller anterior-posterior cross-vein distance as compared to wild-type controls.

Expression of ds^Scer\UAS.cTa under the control of Scer\GAL4^hh.PU in a ds^38k/ds^36D background leads to high lethality during early pupal stages, with only a few adult survivors that show severe wing defects, as compared to ds^38k/ds^36D controls.

(Revilla-Yates et al., 2015)

Overexpression of ds^Scer\UAS.cTa under the control of Scer\GAL4^Bx-MS1096 results in wing shape, posterior ridge and wing hair polarity phenotypes.

Overexpression of ds^Scer\UAS.cTa under the control of Scer\GAL4^salm-459.2 does not affect wing hair polarity.

(Hogan et al., 2011)

At 24 hours after puparium formation, the majority of microtubules in wing cells within the expression domain of animals co-expressing ds^Scer\UAS.cTa and ds^EP-822 under the control of Scer\GAL4^en-e16E in the posterior wing compartment align themselves along the proximodistal axis, as occurs in wild-type wing cells. Wing hair polarity in the posterior compartment is not disrupted in the mutant adults, but the adjacent anterior cells (up to 5-6 cells) misorient their hairs away from the anterior/posterior compartment boundary.

(Harumoto et al., 2010)

Generalized overexpression of ds^Scer\UAS.cTa under the control of Scer\GAL4^arm.PS disrupts denticle belt row structure and disturbs denticle polarity, most strongly in the A compartment.

Overexpression of ds^Scer\UAS.cTa in the P compartment (under the control of Scer\GAL4^en-e16E) depolarizes denticle belt row 2 while row 3 polarity is reversed to now point forwards. This effet on row 3 indicates that there has been a propagation of a polarity change to the next cell.

Scer\GAL4^ptc-559.1-led expression of ds^Scer\UAS.cTa depolarized denticle belt row 2 in the third stage larva. Both rows 1 and 3 point up towards the neighboring cells of row 2 and, in both, their polarity is reversed from that of the wild-type. Rows 4-6 are unaffected. Row 0, as is normal, points forwards. These changes in polarity are not accompanied by any gross changes in denticle shape or size suggesting that they retain their proper identity.

(Repiso et al., 2010)

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) that are expressing ds^Scer\UAS.cTa under the control of Scer\GAL4^αTub84B.PL in a wild-type background can result in a reversal of cell polarity in wild-type cells posterior to the clone (hairs behind the clone point forwards). This phenotype is weak.

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) that are expressing ds^Scer\UAS.cTa under the control of Scer\GAL4^αTub84B.PL in a ds^unspecified background do not change the cell polarity of cells surrounding the clone.

(Casal et al., 2006)

S2 cells that express ds^Scer\UAS.cTa under the control of Scer\GAL4^Act5C.PI, aggregate.

Expression of ds^Scer\UAS.cTa in the wing causes hairs to orient away from the region of misexpression when driven by Scer\GAL4^salm-459.2, Scer\GAL4^Dll-md23 or Scer\GAL4^en-e16E.

Wings that express ds^Scer\UAS.cTa under the control of Scer\GAL4^αTub84B.PL causes a foreshortening along the proximodistal axis of the wing blade as the anterior and posterior crossveins are abnormally close.

(Matakatsu and Blair, 2006)

ds^Scer\UAS.cTa; Scer\GAL4^Act5C transfected S2 cells do not aggregate. ds^Scer\UAS.cTa; Scer\GAL4^salm-459.2 wings have minor planar polarity defects, with a small region proximal to the posterior crossvein being most severely effected, and a region distal to the anterior crossvein, which is truncated, being more mildly affected. ds^Scer\UAS.cTa; Scer\GAL4^en-e16E also have only mild planar polarity, restricted to a region of the proximal wing immediately anterior to the anterior-posterior compartment boundary. ds^Scer\UAS.cTa; Scer\GAL4^Dll-md23 wings have a more severe planar polarity phenotype, with the normal wing hair polarity largely restricted to the regions close to the anterior-posterior boundary apart from its distal tip. ds^Scer\UAS.cTa; Scer\GAL4^αTub84B.PP wings, by contrast, have completely normal wing hair planar polarity. Experiments using Scer\GAL80^ts.αTub84B indicate that the expression of ds using ds^Scer\UAS.cTa; Scer\GAL4^salm-459.2 is only able to disrupt planar cell polarity in adult wings when it occurs in a window around late larval and early pupal stages (before 18 hours after puparium formation).

(Matakatsu and Blair, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^salm-459.2, ds^UAS.cTa has abnormal planar polarity phenotype, enhanceable by fj^d1/fj^d1

(Matakatsu and Blair, 2004)

Suppressed by

Statement

Reference

Scer\GAL4^Act5C.PI, ds^UAS.cTa has abnormal planar polarity | somatic clone | adult stage phenotype, suppressible by dachs¹/dachs^GC13

(Brittle et al., 2012)

NOT Suppressor of

Statement

Reference

ds^UAS.cTa/Scer\GAL4^GH146 is a non-suppressor of abnormal neuroanatomy | somatic clone | cell autonomous phenotype of Tbcd¹

(Okumura et al., 2015)

Scer\GAL4^Act.PU/ds^UAS.cTa is a non-suppressor of abnormal planar polarity phenotype of ft⁸/ft^G-rv

(Matakatsu and Blair, 2012)

Other

Statement

Reference

Scer\GAL4^Bx-MS1096, ds^UAS.cTa, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, ds^UAS.cTa, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ds^UAS.cTa, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ds^UAS.cTa, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^salm-459.2, ds^UAS.cTa has wing hair phenotype, enhanceable by fj^d1/fj^d1

(Matakatsu and Blair, 2004)

Suppressed by

Statement

Reference

Scer\GAL4^Act5C.PI, ds^UAS.cTa has ommatidium | somatic clone | adult stage phenotype, suppressible by dachs¹/dachs^GC13

(Brittle et al., 2012)

NOT Suppressor of

Statement

Reference

ds^UAS.cTa/Scer\GAL4^GH146 is a non-suppressor of adult antennal lobe projection neuron DL1 adPN | somatic clone | cell autonomous phenotype of Tbcd¹

(Okumura et al., 2015)

Other

Statement

Reference

Scer\GAL4^Bx-MS1096, ds^UAS.cTa, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ds^UAS.cTa, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

Scer\GAL4^Act5C.PU, ds^UAS.cTa, ft^UAS.cMa has S2 cell-line phenotype

(Matakatsu and Blair, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ds^Scer\UAS.cTa under the control of Scer\GAL4^GH146 does not rescue the defects seen in TBCD¹ mutant DL1 projection neuron clones.

(Okumura et al., 2015)

Expression of ds^Scer\UAS.cTa under the control of Scer\GAL4^Act.PU in ft^G-rv/ft⁸ animals does not detectably improve hair planar cell polarity defects in the abdomen.

(Matakatsu and Blair, 2012)

When ds^Scer\UAS.cTa is overexpressed under the control of Scer\GAL4^Bx-MS1096 in a pk³⁰ mutant wing, the pk³⁰ wing hair phenotype is modified to a more distal polarity in the anterior and distal posterior regions.

Overexpression of ds^Scer\UAS.cTa under the control of Scer\GAL4^salm-459.2 in a pk³⁰ mutant wings results in changes in wing hair polarity (relative to the wild-type pattern) outside of the main Scer\GAL4^salm-459.2 expression domain.

(Hogan et al., 2011)

Clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are doubly mutant for ds^unspecified and ft^unspecified and are also expressing ds^Scer\UAS.cTa under the control of Scer\GAL4^αTub84B.PL can reverse the cell polarity of cells posterior to the clone.

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are co-expressing both fj^Scer\UAS.cZa and ds^Scer\UAS.cTa under the control of Scer\GAL4^αTub84B.PL result in a reversal of cell polarity in cells anterior to the clone.

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are co-expressing both ft^Scer\UAS.cMa and ds^Scer\UAS.cTa under the control of Scer\GAL4^αTub84B.PL result in a reversal of cell polarity in cells anterior to the clone.

The weak reversal of cell polarity phenotype that is seen in cells posterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are expressing ds^Scer\UAS.cTa under the control of Scer\GAL4^αTub84B.PL is not enhanced if the clones are induced in a fj^unspecified background compared to a wild-type background.

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are co-expressing both fz^Scer\UAS.cUa and ds^Scer\UAS.cTa under the control of Scer\GAL4^αTub84B.PL and the clones have been induced in a ds^unspecified background, result in reversal of cell polarity in cells anterior to the clone.

(Casal et al., 2006)

S2 cells co-transfected with ft^Scer\UAS.cMa and ds^Scer\UAS.cTa, under the control of Scer\GAL4^Act5C.PI, aggregate, as do cells co-transfected with ds^Scer\UAS.cTa and ft^{ΔICD.Scer\UAS.T:Ivir\HA}.

(Matakatsu and Blair, 2006)

S2 cells co-transfected with ds^Scer\UAS.cTa, ft^Scer\UAS.cMa and Scer\GAL4^Act5C aggregate into clusters of about 50 cells. This aggregation occurs even if 2 groups of cells are transfected separately with ds^Scer\UAS.cTa; Scer\GAL4^Act5C or ft^Scer\UAS.cMa; Scer\GAL4^Act5C and then mixed. Formation of these aggregates is inhibited by the presence of EGTA. Co-transfection of fj^Scer\UAS.cZa with ds^Scer\UAS.cTa, ft^Scer\UAS.cMa and Scer\GAL4^Act5C into S2 cells has no detectable effect on their aggregation, or the sensitivity of aggregates to agitation. The planar polarity defects seen in the wings of ds^Scer\UAS.cTa; Scer\GAL4^salm-459.2 flies are mildly enhanced by fj^d1/fj^d1 : polarity defects are seen outside the regions of the wing affected by ds^Scer\UAS.cTa; Scer\GAL4^salm-459.2 alone.

(Matakatsu and Blair, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^ds-gal4/ds^UAS.cTa partially rescues ds⁰⁵¹⁴²

(Matakatsu and Blair, 2006)

ds^UAS.cTa/Scer\GAL4^αTub84B.PL partially rescues ds⁰⁵¹⁴²

(Matakatsu and Blair, 2006)

Scer\GAL4^αTub84B.PP/ds^UAS.cTa partially rescues ds⁰⁵¹⁴²

(Matakatsu and Blair, 2004)

Fails to rescue

ds^UAS.cTa fails to rescue ds^38k/ds^D36

(Revilla-Yates et al., 2015)

Comments

Expression of ds^Scer\UAS.cTa under the control of Scer\GAL4^hh.PU fails to rescue the wing defects of ds^38k/ds^36D adults.

(Revilla-Yates et al., 2015)

Expression of ds^Scer\UAS.cTa, under the control of Scer\GAL4^αTub84B.PL, rescues the planar cell polarity defects of ds⁰⁵¹⁴² wings in all areas except the most proximal regions of the wing blade. Expression driven by Scer\GAL4^ds-gal4 partially rescues planar cell polarity in the proximal wing but does not fully rescue the loss of the crossveins.

(Matakatsu and Blair, 2006)

Uniform ds^Scer\UAS.cTa expression using Scer\GAL4^αTub84B.PP rescues the planar cell polarity defects of ds⁰⁵¹⁴²/ds⁰⁵¹⁴² flies in the distal region between L3 and L4, but not in the proximal region just posterior to L1.

(Matakatsu and Blair, 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Takara

(Matakatsu and Blair, 2004)

Comments

Carried in a plasmid and transfected into S2 cells for use in cell aggregation assays.

(Matakatsu and Blair, 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ds^Scer\UAS.cTa

(Casal et al., 2006)

ds^UAS.cTa

Name Synonyms

Saccharomyces cerevisiae UAS construct of Takara

(Matakatsu and Blair, 2004)

Secondary FlyBase IDs

References (20)

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