FlyBase Allele Report: Dmel\Nos[C]

General Information

Symbol

Dmel\Nos^C

Species

D. melanogaster

Name

FlyBase ID

FBal0182479

Feature type

allele

Associated gene

Dmel\Nos

Associated Insertion(s)

Carried in Construct

Also Known As

dNOS^C

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Yakubovich et al., 2010)

Mutagen

ethyl methanesulfonate

(Regulski et al., 2004)

Progenitor genotype

Cytology

Description

Amino acid replacement: G585E.

(Regulski et al., 2004)

Nucleotide substitution: G1942A.

(Regulski et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:10,816,228..10,816,228 [-]

Nucleotide change:

G10816228A

Reported nucleotide change:

G?A

Amino acid change:

G584E | Nos-PA; G584E | Nos-PF; G333E | Nos-PK

Reported amino acid change:

G585E

(Regulski et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | P-stage (with Nos¹)

(Rabinovich et al., 2016)

abnormal neuroanatomy | P-stage (with Nos^Δ15)

(Rabinovich et al., 2016)

abnormal neurophysiology | third instar larval stage

(Robinson et al., 2018)

viable

(Yakubovich et al., 2010)

viable (with Nos^Δ15)

(Yakubovich et al., 2010)

Phenotype Manifest In

adult mushroom body gamma-lobe | P-stage (with Nos¹)

(Rabinovich et al., 2016)

adult mushroom body gamma-lobe | P-stage (with Nos^Δ15)

(Rabinovich et al., 2016)

axon | P-stage (with Nos¹)

(Rabinovich et al., 2016)

axon | P-stage (with Nos^Δ15)

(Rabinovich et al., 2016)

neuromuscular junction | third instar larval stage

(Robinson et al., 2018)

ventral longitudinal muscle cell | third instar larval stage

(Robinson et al., 2018)

Detailed Description

Statement

Reference

Nos^C third instar larvae exhibit significantly reduced paired pulse ratio at 20 ms ISI; significantly enhanced mEJC frequencies; and significantly increased vesicle pool size in NMJs of ventral longitudinal m6 in abdominal segments 2 and 3 when compared to controls.

(Robinson et al., 2018)

A subset of Nos^Δ15/Nos^C or Nos^C/Nos¹ mutants display pruning defects and/or precocious regrowth of axons in the mushroom body gamma-lobe, as compared to Nos^Δ15/+, Nos¹/+ or Nos^C/+ controls.

(Rabinovich et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

FlyBase curator comment: FBrf0210138 demonstrates that the lethality attributed to the Nos^C mutation in FBrf0180083 is in fact due to a separable second-site lethal on the chromosome.

(Regulski et al., 2004)

Separable from: a secondary lethal mutation on the chromosome, which maps roughly 0.08 map units (approximately 22kb) from the Nos^C lesion.

(Yakubovich et al., 2010)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

NOS^C

(Robinson et al., 2018)

Nos^C

(Kuntz et al., 2017, Yakubovich et al., 2010)

dNOS^C

(Rabinovich et al., 2016, Pilgram et al., 2011, Regulski et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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