FlyBase Allele Report: Dmel\Duox[RNAi.UAS]

General Information

Symbol

Dmel\Duox^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0190061

Feature type

allele

Associated gene

Dmel\Duox

Associated Insertion(s)

Carried in Construct

P{UAS-Duox.RNAi}

Also Known As

UAS-Duox-RNAi, UAS-Duox-IR, UAS-duox RNAi, Duox RNAi, UAS-Duox^RNAi

Key Links

Transgenic product class

RNAi_reagent

(Ha et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ha et al., 2005)

Mutagen

in vitro construct

(Ha et al., 2005, Ha et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Duox.RNAi}

Cytology

Description

UASt regulatory sequences drive expression of a head-to-tail inverted repeat of a 510bp fragment encoding amino acids 976 to 1145 of Duox. The inverted repeats are separated by an intronic spacer.

(Ha et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Duox

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^cad-em459

(Ha et al., 2005, Ha et al., 2005)

abnormal immune response, with Scer\GAL4^da.G32

(Ha et al., 2009, Ha et al., 2009, Ha et al., 2005, Ha et al., 2005)

chemical resistant, with Scer\GAL4^Uro.PU

(Li et al., 2020)

increased cell death | third instar larval stage, with Scer\GAL4^en.PU

(Anh et al., 2011)

lethal, with Scer\GAL4^Act5C.PU

(Anh et al., 2011)

lethal, with Scer\GAL4^Tub.PU

(Anh et al., 2011)

lethal - all die before end of pupal stage, with Scer\GAL4^c179

(Anh et al., 2011)

short lived | nutrition conditional, with Scer\GAL4^da.G32

(Ha et al., 2009)

some die during pupal stage, with Scer\GAL4^c179

(Anh et al., 2011)

visible, with Scer\GAL4^ap.PU

(Hurd et al., 2015)

Phenotype Manifest In

ommatidium, with Scer\GAL4^GMR.PU

(Anh et al., 2011)

wing, with Scer\GAL4^ap.PU

(Hurd et al., 2015)

wing, with Scer\GAL4^Bx-MS1096

(Anh et al., 2011)

wing, with Scer\GAL4^en.PU

(Anh et al., 2011)

wing vein, with Scer\GAL4^en.PU

(Anh et al., 2011)

Detailed Description

Statement

Reference

Expression of Duox^dsRNA.UAS under the control of Scer\GAL4^Uro.PU leads to improved fly resistance to paraquat.

(Li et al., 2020)

Expression of Duox^dsRNA.UAS under the control of Scer\GAL4^NP0001 in axenic adults colonized with Lp[SD] (Lp isolated from conventionally raised PGRP-SD^sk1 homozygotes) do not exhibit any changes in the occurrence of cell division in gut when compared to controls.

Expression of Duox^dsRNA.UAS under the control of Scer\GAL4^NP0001 does not lead to any changes in adult life span when compared to controls.

(Iatsenko et al., 2018)

Expression of Cy^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ap.PU causes a slight downward curvature or cupping of the wing. Ultrastructurally, the dorsal and ventral cuticles are rarely in direct contact, but are instead separated by a gap filled with disordered material.

(Hurd et al., 2015)

Expression of Duox^{dsRNA.Scer\UAS} using Scer\GAL4^GMR.PU results in dark circles inside ommatidia.

Expression of Duox^{dsRNA.Scer\UAS} using Scer\GAL4^ey.PU, Scer\GAL4^tey-5053A or Scer\GAL4^rho.PL does not produce detectable phenotypes.

Expression of Duox^{dsRNA.Scer\UAS} using Scer\GAL4^en.PU or Scer\GAL4^Bx-MS1096 results in held-out and fragile wings. With Scer\GAL4^en.PU, wing veins are also indistinct and the posterior compartment is paler.

In flies containing 2 copies of both Scer\GAL4^en.PU and Duox^{dsRNA.Scer\UAS}, the posterior area of wings appears lightly crinkled and damaged around 3 days after hatching. After 6 days, the damage increases in severity. Penetrance is 100% after 6 days.

Expression of Duox^{dsRNA.Scer\UAS} using Scer\GAL4^en.PU eliminates reactive oxygen species in the posterior compartment of the adult wing.

Expression of Duox^{dsRNA.Scer\UAS} using Scer\GAL4^en.PU results in increased apoptosis in the posterior compartment of the wing disc.

The amino acid composition of and the average amount of amino acids in Duox^{dsRNA.Scer\UAS}, Scer\GAL4^en.PU adult wings is similar to controls. However, the catechol component (which is an important precursor in cuticular sclerotization and melanization) in the mutant wings is significantly lower than in controls wings. Mutant wings also show a decreased dityrosine component in hydrolysis samples, indicating reduced dityrosine linkages in the cuticle.

(Anh et al., 2011)

Flies expressing Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 show a normal lifespan on dead yeast medium, but show reduced survival compared to controls on live yeast medium.

Flies expressing Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 and raised on live yeast medium for 1 hour have a similar number of living yeast in the gut (assayed as the number of colony-forming units (CFUs) from homogenates of surface-sterilized intestines) immediately after the live yeast feeding period, but in contrast to wild-type flies (where the number of initial CFUs decreases to a low level starting from 24 hours after ingestion), the initial CFUs in the mutant gut are maintained for 48 hours after ingestion and increase steadily thereafter.

(Ha et al., 2009)

Flies expressing Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 show reduced survival after gut infection (via feeding) with Erwinia carotovora carotovora strain 15 compared to control flies.

(Ha et al., 2009)

Flies expressing Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^cad-em459 show a reduced survival rate compared to control flies when naturally infected with E.coli, S.cerevisiae, M.luteus or S.typhimurium. Flies expressing Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 show a reduced survival rate compared to control flies when naturally infected with "Ecc15" (P.carotovorum.carotovorum).

(Ha et al., 2005)

Flies expressing Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 or Scer\GAL4^cad-em459 show a reduced survival rate compared to control flies when naturally infected with "Ecc15" (P.carotovorum.carotovorum). Flies expressing Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^c564 show a normal survival rate when naturally infected with "Ecc15" (P.carotovorum.carotovorum). Ingested "Ecc15" (P.carotovorum.carotovorum) bacteria persist and are able to proliferate throughout the intestinal tracts of flies expressing Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32, in contrast to normal flies.

(Ha et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Duox^RNAi.UAS, Scer\GAL4^en.PU has increased cell death | third instar larval stage phenotype, suppressible by Df(3L)H99/+

(Anh et al., 2011)

Duox^RNAi.UAS, Scer\GAL4^cad-em459 has abnormal immune response phenotype, suppressible by Irc^RNAi.UAS, Scer\GAL4^cad-em459

(Ha et al., 2005)

Duox^RNAi.UAS, Scer\GAL4^cad-em459 has abnormal immune response phenotype, suppressible by Hsap\DUOX1^UAS.cHa, Scer\GAL4^cad-em459

(Ha et al., 2005)

Duox^RNAi.UAS, Scer\GAL4^cad-em459 has abnormal immune response phenotype, suppressible by Hsap\DUOX2^UAS.cHa, Scer\GAL4^cad-em459

(Ha et al., 2005)

NOT Enhancer of

Statement

Reference

Scer\GAL4^NP0001/Duox^RNAi.UAS is a non-enhancer of increased occurrence of cell division | adult stage phenotype of PGRP-SD^sk1

(Iatsenko et al., 2018)

Scer\GAL4^NP0001/Duox^RNAi.UAS is a non-enhancer of short lived phenotype of PGRP-SD^sk1

(Iatsenko et al., 2018)

Suppressor of

Statement

Reference

Scer\GAL4^Uro.PU/Duox^RNAi.UAS is a suppressor of abnormal immune response phenotype of mat^SK1

(Li et al., 2020)

Scer\GAL4^Uro.PU/Duox^RNAi.UAS is a suppressor of increased mortality | conditional phenotype of mat^SK1

(Li et al., 2020)

Duox^RNAi.UAS, Scer\GAL4^da.G32 is a suppressor of short lived phenotype of Mkp3^GD8219, Scer\GAL4^da.G32

(Ha et al., 2009)

Duox^RNAi.UAS, Scer\GAL4^da.G32 is a suppressor of increased cell death phenotype of Mkp3^GD8219, Scer\GAL4^da.G32

(Ha et al., 2009)

Duox^RNAi.UAS, Scer\GAL4^cad-em459 is a suppressor of abnormal immune response phenotype of Irc^RNAi.UAS, Scer\GAL4^cad-em459

(Ha et al., 2005)

NOT Suppressor of

Statement

Reference

Scer\GAL4^NP0001/Duox^RNAi.UAS is a non-suppressor of increased occurrence of cell division | adult stage phenotype of PGRP-SD^sk1

(Iatsenko et al., 2018)

Scer\GAL4^NP0001/Duox^RNAi.UAS is a non-suppressor of short lived phenotype of PGRP-SD^sk1

(Iatsenko et al., 2018)

Phenotype Manifest In

Suppressed by

Statement

Reference

Duox^RNAi.UAS, Scer\GAL4^en.PU has wing phenotype, suppressible by Df(3L)H99/+

(Anh et al., 2011)

Duox^RNAi.UAS, Scer\GAL4^en.PU has wing vein phenotype, suppressible by Df(3L)H99/+

(Anh et al., 2011)

NOT Enhancer of

Statement

Reference

Scer\GAL4^NP0001/Duox^RNAi.UAS is a non-enhancer of adult gut phenotype of PGRP-SD^sk1

(Iatsenko et al., 2018)

Suppressor of

Statement

Reference

Duox^RNAi.UAS, Scer\GAL4^da.G32 is a suppressor of adult posterior midgut epithelium phenotype of Mkp3^GD8219, Scer\GAL4^da.G32

(Ha et al., 2009)

NOT Suppressor of

Statement

Reference

Scer\GAL4^NP0001/Duox^RNAi.UAS is a non-suppressor of adult gut phenotype of PGRP-SD^sk1

(Iatsenko et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Df(3L)H99/+ suppresses the adult wing phenotype and the increased apoptosis in posterior wing discs seen in Duox^{dsRNA.Scer\UAS}, Scer\GAL4^en.PU flies.

(Anh et al., 2011)

The shortened lifespan and increased apoptosis seen in the posterior midgut of flies expressing Mkp3^GD8219 under the control of Scer\GAL4^da.G32 is significantly rescued by co-expression of Duox^{dsRNA.Scer\UAS}.

(Ha et al., 2009)

Flies co-expressing Irc^{dsRNA.Scer\UAS} and Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^cad-em459 show a normal survival rate when naturally infected with "Ecc15" (P.carotovorum.carotovorum).

(Ha et al., 2005)

Xenogenetic Interactions

Statement

Reference

The survival rates of flies expressing Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^cad-em459 after natural infection with "Ecc15" (P.carotovorum.carotovorum) are markedly improved if they co-express either Hsap\DUOX1^Scer\UAS.cHa or Hsap\DUOX2^Scer\UAS.cHa.

(Ha et al., 2005)

Complementation and Rescue Data

Rescued by

Duox^RNAi.UAS is rescued by Scer\GAL4^da.G32/Duox^UAS.cHa

(Ha et al., 2009)

Duox^RNAi.UAS is rescued by Scer\GAL4^da.G32/Duox^UAS.cHa

(Ha et al., 2005)

Duox^RNAi.UAS is rescued by Duox^UAS.cHa/Scer\GAL4^cad-em459

(Ha et al., 2005)

Not rescued by

Duox^RNAi.UAS is not rescued by Duox^ΔPHD.UAS/Scer\GAL4^da.G32

(Ha et al., 2005)

Duox^RNAi.UAS is not rescued by Duox^ΔPHD.UAS/Scer\GAL4^cad-em459

(Ha et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Cy^{dsRNA.Scer\UAS}

Duox^RNAi.UAS

Duox^RNAi

(Tamamouna et al., 2021)

Duox^{dsRNA.Scer\UAS}

Duox^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (21)

Report Sections

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