FlyBase Allele Report: Dmel\Cks85A[ex15]

General Information

Symbol

Dmel\Cks85A^ex15

Species

D. melanogaster

Name

FlyBase ID

FBal0190414

Feature type

allele

Associated gene

Dmel\Cks85A

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Ghorbani et al., 2011)

Mutagen

Progenitor genotype

Cytology

Description

Excision of P{EP}Cap-G^EP2346

(Swan et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous larvae develop more slowly than wild type and after an extended third larval instar they die after pupariation, prior to the differentiation of adult structures.

The salivary glands of feeding third instar Cks85A^ex15/Df(3R)BSC197 larvae are significantly smaller and have less nuclei undergoing DNA replication than those of controls. The ploidy of salivary gland cell nuclei is reduced in homozygous wandering third instar larvae compared to wild type.

The brains of homozygous third instar larvae are smaller than those of controls and some of the cells appear larger than normal. Approximately 6% of the cells are tetraploid and less than 1% have a higher ploidy.

Differentiated cells posterior to the morphogenetic furrow appear normal in homozygous eye discs but cells anterior the furrow have larger nuclei than normal which appear polyploid. This phenotype is more severe at higher temperature.

Flow cytometry indicates that wing discs from newly wandering homozygous third instar larvae contain a population of tetraploid (8n) cells. This population is more prominent at higher temperatures. A small but reproducible peak corresponding to 16n cells appears at 29[o]C. Extensive apoptosis is seen in the homozygous wing discs.

Expression of Cks85A^GD13737 under the control of Scer\GAL4^en-e16E in a Cks85A^ex15/+ background at 29[o]C leads to increased spacing of the wing hairs near the anterior/posterior compartment boundary.

Heterozygous flies derived from homozygous female germline clones are viable and appear phenotypically normal. Homozygous animals derived from homozygous female germline clones survive to the larval/pupal transition and show the same degree of polyploidy as maternally rescued larvae raised in parallel.

(Ghorbani et al., 2011)

Cks85A^ex15 mutants grow more slowly than wild-type and are lethal at the third instar/pupal transition. This lethality can be rescued through ectopic expression of Cks85A^{Scer\UAS.T:Zzzz\FLAG}, under the control of Scer\GAL4^da.G32. Cks85A^ex15 mutants do not exhibit any obvious mitotic defects in zygotic tissues. Germline clone analysis of Cks85A^ex15 reveals that Cks85A is not required maternally.

(Swan et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Cks85A^ex15 has abnormal mitotic cell cycle | third instar larval stage phenotype, enhanceable by Skp2[+]/Skp2^ex9

(Ghorbani et al., 2011)

Suppressed by

Statement

Reference

Cks85A^ex15 has abnormal mitotic cell cycle | third instar larval stage phenotype, suppressible by Cks30A^KO/Cks30A^KO

(Ghorbani et al., 2011)

Cks85A^ex15 has lethal | P-stage phenotype, suppressible | partially by Skp2^UASp.Tag:HA/Scer\GAL4^da.G32

(Ghorbani et al., 2011)

Cks85A^ex15 has abnormal mitotic cell cycle | third instar larval stage phenotype, suppressible by Skp2^UASp.Tag:HA/Scer\GAL4^da.G32

(Ghorbani et al., 2011)

Cks85A^ex15 has increased cell death | third instar larval stage phenotype, suppressible by Scer\GAL4^en-e16E/BacA\p35^UAS.cHa

(Ghorbani et al., 2011)

NOT suppressed by

Statement

Reference

Cks85A^ex15 has abnormal mitotic cell cycle | third instar larval stage phenotype, non-suppressible by Cks30A^UAS.Tag:FLAG/Scer\GAL4^da.G32

(Ghorbani et al., 2011)

Other

Statement

Reference

Cks85A^ex15, Scer\GAL4^da.G32, Skp2^UASp.Tag:HA has lethal | pharate adult stage phenotype

(Ghorbani et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cks85A^ex15 has eye disc | third instar larval stage phenotype, enhanceable by Skp2[+]/Skp2^ex9

(Ghorbani et al., 2011)

Suppressed by

Statement

Reference

Cks85A^ex15 has eye disc | third instar larval stage phenotype, suppressible by Cks30A^KO/Cks30A^KO

(Ghorbani et al., 2011)

Cks85A^ex15 has eye disc | third instar larval stage phenotype, suppressible by Skp2^UASp.Tag:HA/Scer\GAL4^da.G32

(Ghorbani et al., 2011)

Cks85A^ex15 has wing disc | third instar larval stage phenotype, suppressible by Scer\GAL4^en-e16E/BacA\p35^UAS.cHa

(Ghorbani et al., 2011)

NOT suppressed by

Statement

Reference

Cks85A^ex15 has eye disc | third instar larval stage phenotype, non-suppressible by Cks30A^UAS.Tag:FLAG/Scer\GAL4^da.G32

(Ghorbani et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The polyploidy seen in the eye discs of homozygous Cks85A^ex15 wandering third instar larvae anterior to the morphogenetic furrow is suppressed in Cks85A^ex15 Cks30A^KO double homozygotes, but is not suppressed by expression of Cks30A^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^da.G32.

The polyploidy seen in the eye discs of homozygous Cks85A^ex15 wandering third instar larvae anterior to the morphogenetic furrow is enhanced by Skp2^ex9/+.

Cks85A^ex15, Skp2^ex9 double homozygotes display the same lethal phase and same degree of polyploidy in cells anterior to the morphogenetic furrow in the eye disc as Skp2^ex9 single mutants.

Expression of Skp2^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 partially rescues the lethality of Cks85A^ex15 homozygotes: the animals progress to the pharate adult stage and die during or shortly after escaping from the pupal case.

Expression of Skp2^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 rescues the polyploidy seen anterior to the morphogenetic furrow in the eye discs of homozygous Cks85A^ex15 wandering third instar larvae at 29[o]C.

(Ghorbani et al., 2011)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^en-e16E in the posterior compartment suppresses the apoptosis seen in Cks85A^ex15 third larval instar wing discs.

(Ghorbani et al., 2011)

Complementation and Rescue Data

Rescued by

Cks85A^ex15 is rescued by Scer\GAL4^da.G32/Cks85A^UAS.Tag:FLAG

(Ghorbani et al., 2011)

Cks85A^ex15 is rescued by Scer\GAL4^da.G32/Cks85A^UAS.Tag:FLAG

(Swan et al., 2005)

Comments

Expression of Cks85A^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^da.G32 rescues the polyploidy seen in nuclei anterior to the morphogenetic furrow in the eye discs of Cks85A^ex15 homozygous larvae.

(Ghorbani et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58999

w^*; Cks85A^ex15/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Cks85A^ex15

(Vasavan et al., 2024, Ghorbani et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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