FlyBase Allele Report: Dmel\Dronc[51]

Nc^I24/Nc⁵¹ and Nc^I29/Nc⁵¹ transheterozygotes exhibit a delay in vCrz neuron apoptosis. At 7 hours after puparium formation 75% vCrz neurons are still present although these all die by 48 hours after puparium formation.

(Lee et al., 2011)

S2P^KG08356/S2P¹, Nc⁵¹/+ and S2P^KG08356/S2P¹, Nc⁵¹/Nc⁵¹ mutant larvae exhibit lethality between second and third instar stages. Lethality is rescued partially by supplementing fly food with fatty acids, but viability is still reduced compared to S2P^KG08356/S2P¹ mutants.

(Amarneh et al., 2009)

Nc⁵¹ mosaic wings retain Disc\RFP^{hs.DsRedT4.T:nls5}-positive wing epithelial cells 4-11 days after eclosion, whereas control epithelial cells undergo apoptosis moments after eclosion.

Wing blemishes are not seen in Nc⁵¹ heterozygotes.

(Link et al., 2007)

Programmed cell death inCrz-expressing neurons in the ventral nerve cord is significantly delayed in Nc⁵¹/Nc^I24 mutants. Approximately 12 neurons still survive at 7 hours after pupal formation, while the number is reduced to approximately 8 at 16 hours after pupal formation, and none at 48 hours after pupal formation.

Nc⁵¹/Nc^I29 mutants exhibit a delay in programmed cell death in Crz-expressing neurons in the ventral nerve cord during pupation.

(Choi et al., 2006)

Dendrites of Nc⁵¹ mutant C4da neuron clones appear normal at larval stages. However, unlike wild-type, these neurons fail to properly prune their larval dendrites during metamorphosis and most display relatively intact primary and secondary larval dendritic arbors at 18 hours after puparium formation.

(Kuo et al., 2006)

Nc²/Nc⁵¹ mutant third instar larvae have melanotic nodules found in the hemocoel or in association with He-positive lymph glands. They also show gut melanisation not accompanied by tissue overgrowth or hemocyte encapsulation.

(Minakhina and Steward, 2006)

Nc⁵¹ homozygous embryos hatch at the normal time, but the larvae develop more slowly than wild-type. These larvae fail to pupariate, but continue to grow for some time after controls pupariate. The resulting giant larvae die at 10-12 days after wild-type larvae pupariate. Unlike wild-type larvae, these larvae are not induced to pupariate early by feeding with ecdysone at the mid-third instar.

Nc⁵¹ homozygous larvae fail to exhibit cell death in the larval midgut ,as wild-type larvae do, at end of the third larval instar (assayed by Tunel staining) or in response to feeding with ecdysone at mid-third instar (assayed by acridine orange staining).

(Waldhuber et al., 2005)

Nc⁵¹ homozygous embryos display head involution defects. Post-embryonic growth of these animals is slower than in wild-type and both larval moults and pupation are delayed. However, once they do reach the wandering late third instar larval stage, these larvae are generally bigger wild-type counterparts and have larger and sometimes deformed discs and brain. They also exhibit hyperplasia of the blood cells. These animals die during early pupal stages, sometimes with visible melanotic tumors.

Nc⁵¹ homozygous embryos laid by Nc^KG02994/Nc⁵¹ mothers do not hatch and have more severe head involution defects than Nc⁵¹ homozygotes laid by wild-type mothers. These have embryos decreased levels of cell death, as assayed by tunel staining, in the epidermis at stage 12 and the ventral nerve cord at stage 15/16. Consistent with a reduction in cell death, increases in cell number are see in multiple cases: Extra cells are associated with Bolwig's organ at stage 15 and extra cells are present in the ventral nerve cord at late embryonic stages

Nc^KG02994/Nc⁵¹ adults have a higher frequency of post-alar bristle duplication than Nc^KG02994 homozygous adults.

Adult eyes completely populated by Nc⁵¹ homozygous somatic clone cells are normal sized but rough. This is not due to supernumerary photoreceptor cells, however, ommatidial pre-clusters are irregularly spaced immediately posterior to the furrow in late third instar eye discs of this genotype.

No obvious defects are present in the wings of newly eclosed flies carrying Nc⁵¹ homozygous clones. But, the wings of these flies develop melanized blotches as they age.

Cultured hemocytes isolated from third instar Nc⁵¹ homozygous larvae are much less sensitive to induction of apoptosis by a variety of pro-apoptotic agents (cycloheximide, Actinomycin D, etoposide (Topoisomerase II inhibitor), ethanol, and smac mimetic) than wild-type controls. In addition, levels of constitutive apoptosis in these cells are significantly lower than controls.

(Chew et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Dronc^I24/Dronc⁵¹ has abnormal cell death phenotype, enhanceable by Strica⁴

(Lee et al., 2011)

Suppressed by

Statement

Reference

Dronc^I24/Dronc⁵¹ has abnormal cell death phenotype, suppressible by Dredd^L23

(Lee et al., 2011)

Enhancer of

Statement

Reference

Dronc⁵¹ is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^insc-Mz1407, numb^S2D.UAS

(Ouyang et al., 2011)

Suppressor of

Statement

Reference

Dronc⁵¹/Dronc[+] is a suppressor | partially of decreased size | adult stage phenotype of Scer\GAL4^nub.PU, syd^GLC01419

(Ahmad et al., 2021)

Dronc⁵¹/Dronc[+] is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^nub.PU, syd^GLC01419

(Ahmad et al., 2021)

Dronc⁵¹/Nc[+] is a suppressor of abnormal neurophysiology phenotype of ninaE¹⁷, ninaE^P37H

(Griciuc et al., 2014)

Dronc⁵¹/Nc[+] is a suppressor of abnormal neuroanatomy phenotype of ninaE¹⁷, ninaE^P37H

(Griciuc et al., 2014)

Dronc⁵¹/Nc[+] is a suppressor of viable | conditional phenotype of S2P^KG08356/S2P¹

(Amarneh et al., 2009)

Dronc⁵¹/Dronc⁵¹ is a suppressor of viable | conditional phenotype of S2P^KG08356/S2P¹

(Amarneh et al., 2009)

NOT Suppressor of

Statement

Reference

Dronc⁵¹ is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of fzy¹/fzy⁵⁰³²

(Kuang et al., 2014)

Dronc⁵¹ is a non-suppressor of decreased cell number | larval stage phenotype of fzy¹/fzy⁵⁰³²

(Kuang et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dronc^I24/Dronc⁵¹ has neuron phenotype, suppressible by Dredd^L23

(Lee et al., 2011)

Enhancer of

Statement

Reference

Dronc⁵¹ is an enhancer of neuroblast phenotype of Scer\GAL4^insc-Mz1407, numb^S2D.UAS

(Ouyang et al., 2011)

Dronc⁵¹ is an enhancer of type II neuroblast | increased number phenotype of Scer\GAL4^insc-Mz1407, numb^S2D.UAS

(Ouyang et al., 2011)

Dronc⁵¹/Nc[+] is an enhancer of wing phenotype of Dark^CD4

(Chew et al., 2004)

Suppressor of

Statement

Reference

Dronc⁵¹/Nc[+] is a suppressor of rhabdomere of eye photoreceptor cell phenotype of ninaE¹⁷, ninaE^P37H

(Griciuc et al., 2014)

Dronc⁵¹/Nc[+] is a suppressor of eye phenotype of grim^GMR.PH

(Waldhuber et al., 2005)

Dronc⁵¹/Nc[+] is a suppressor of eye phenotype of rpr^GMR.PH

(Waldhuber et al., 2005)

NOT Suppressor of

Statement

Reference

Dronc⁵¹ is a non-suppressor of neuroblast | larval stage phenotype of fzy¹/fzy⁵⁰³²

(Kuang et al., 2014)

Dronc⁵¹/Nc[+] is a non-suppressor of eye phenotype of hid^GMR.PG

(Waldhuber et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Retinal degeneration and light-response defects are strongly suppressed in ninaE^P37H, ninaE¹⁷, Nc⁵¹/+ flies.

(Griciuc et al., 2014)

Nc⁵¹ does not suppress the neuroblast loss seen in fzy⁵⁰³²/fzy¹ mutant larval brains.

(Kuang et al., 2014)

No viable vCrz neurons are detected at 7 hours after puparium formation in Dredd^L23; Nc^I24/Nc⁵¹ double mutants.

(Lee et al., 2011)

A Nc⁵¹ heterozygous background enhances ectopic neuroblast formation due to numb^S2D.Scer\UAS expression.

(Ouyang et al., 2011)

A Nc⁵¹ heterozygous background enhances the melanotic blemishes found on Ark^CD4 mutant wings.

(Link et al., 2007)

Nc⁵¹/+ partially suppresses the small, rough eye phenotype caused by rpr^GMR.PH or grim^GMR.PH but not that caused by W^GMR.PG.

(Waldhuber et al., 2005)

The melanized wing spot phenotype of Ark^CD4 homozygotes is enhanced by Nc⁵¹/+.

(Chew et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Dronc⁵¹ is partially rescued by Dronc^UAS.EGFP/Scer\GAL4^hs.PB

(Waldhuber et al., 2005)

Dronc⁵¹ is partially rescued by Dronc^+t.drf

(Chew et al., 2004)

Not rescued by

Dronc⁵¹ is not rescued by Dronc^C318S.UAS/Scer\GAL4^hs.PB

(Waldhuber et al., 2005)

Comments

Nc^+t.drf rescues the partially lethality of Nc⁵¹/Nc⁵¹ homozygotes with a few animals rescued to adulthood and up to 50% reaching the pharate adult stage. The delays in larval moults seen in Nc⁵¹/Nc⁵¹ animals are also partially rescued by this transgene.

(Chew et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

23284

y¹ w^*; Dronc⁵¹/TM3, Sb¹

Notes on Origin

Discoverer

Associated with: CG6685⁵¹

(Chew et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

DRONC⁵¹

(Waldhuber et al., 2005)

Dronc51

(Griciuc et al., 2014)

Dronc⁵¹

Dronc^LOF

(Griciuc et al., 2014)

Nc⁵¹

(Kuang et al., 2014)

dron⁵¹

(Lee et al., 2013)

dronc⁵¹

(Ahmad et al., 2021, Verghese et al., 2012, Lee et al., 2011, Amarneh et al., 2009, Link et al., 2007, Minakhina et al., 2007, Choi et al., 2006, Kuo et al., 2006, Minakhina and Steward, 2006, Chew et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (15)

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