FlyBase Allele Report: Dmel\eys[1]

General Information

Symbol

Dmel\eys¹

Species

D. melanogaster

Name

FlyBase ID

FBal0193672

Feature type

allele

Associated gene

Dmel\eys

Associated Insertion(s)

Carried in Construct

Also Known As

spam¹

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Zelhof, 2006)

Mutagen

ethyl methanesulfonate

(Zelhof, 2006)

Progenitor genotype

Cytology

Description

Nucleotide substitution: T957A.

(Zelhof, 2006)

Amino acid replacement: Y319term.

(Zelhof, 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:2,338,961..2,338,961 [+]

Nucleotide change:

T2338961A

Reported nucleotide change:

T957A

Amino acid change:

Y330term | eys-PE; Y330term | eys-PF

Reported amino acid change:

Y319term

Comment:

The difference between the reported and annotated positions of the amino acid change in the mutant results from the use of a downstream translation start in the previous eys annotation and the publication. The annotation was updated in T5.55 to start at an in-frame ATG which is 11aa upstream.

(Zelhof, 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of retinal degeneration

CEA

(Gurudev et al., 2014)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm

(Sehadova et al., 2009)

uncoordinated | adult stage | heat sensitive

(Sehadova et al., 2009)

viable

(Zelhof et al., 2006)

Phenotype Manifest In

eye

(Nie et al., 2012)

ommatidium

(Zelhof et al., 2006)

photoreceptor neuron | chemical conditional

(Gurudev et al., 2014)

photoreceptor neuron | conditional

(Gurudev et al., 2014)

rhabdomere

(Nie et al., 2012, Zelhof et al., 2006)

rhabdomere | P-stage

(Nie et al., 2015)

rhabdomere of eye photoreceptor cell

(Gurudev et al., 2014)

Detailed Description

Statement

Reference

eys¹/eys¹ mutant pupae display fusion of rhabdomeres and a loss of the inter-rhabdomeral lumen.

(Nie et al., 2015)

In eys¹ mutants, all or nearly all rhabdomeres are fused to a single rhabdomere cluster and no inter-rhabdomeral space is formed.

eys¹ mutant photoreceptor cells undergo light-dependent degeneration. The retinal degeneration can be prevented in the absence of dietary vitamin A.

(Gurudev et al., 2014)

One copy of eys¹ does not lead to rhabdomere fusion.

(Nie et al., 2014)

eys¹ mutant eyes exhibit no inter-rhabdomeral space and the rhabdomeres remain juxtaposed to each other.

(Nie et al., 2012)

Mutant files show severe defects in entrainment to temperature cycles in assays studying locomotor rhythms.

Mutant flies show an uncoordinated phenotype at 37[o]C, which is largely suppressed if the humidity is greater than 90%.

(Sehadova et al., 2009)

eys¹ mutant adults have eyes with apparently normal microvilli. However, these eyes show a striking failure of the rhabdomeres to separate from each other, including a complete loss of inter-rhabdomeral space, irregular rhabdomere morphology and improper rhabdomeral contacts.

(Zelhof et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by +/Df(1)ED6829

(Nie et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by Act5C^G0245/Act5C[+]

(Nie et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by zip^HMS01618/Scer\GAL4^GMR.PU

(Nie et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by zip^DN.UAS.GFP/Scer\GAL4^GMR.PU

(Nie et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by sqh^HMS00437/Scer\GAL4^GMR.PU

(Nie et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by sna^HMS01252/Scer\GAL4^GMR.PU

(Nie et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by Rho1^JF02809/Scer\GAL4^GMR.PU

(Nie et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by Rok^JF03225/Scer\GAL4^GMR.PU

(Nie et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by Mbs^N300.UAS/Scer\GAL4^GMR.PU

(Nie et al., 2014)

Suppressed by

Statement

Reference

eys¹ has rhabdomere of eye photoreceptor cell phenotype, suppressible | partially by crb^11A22

(Gurudev et al., 2014)

eys[+]/eys¹, prom¹ has rhabdomere of eye photoreceptor cell phenotype, suppressible by crb^11A22/crb[+]

(Gurudev et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, suppressible by sqh^{E20E21.UAS.cCa}/Scer\GAL4^Pph13.1.6/Scer\GAL4^GMR.PU

(Nie et al., 2014)

eys¹, prom¹ has rhabdomere phenotype, suppressible by Hsap\PROM1^UAS.cNa/Scer\GAL4^GMR.PF/Hsap\EYS^UAS.cNa

(Nie et al., 2012)

eys¹, prom¹ has eye phenotype, suppressible by Hsap\PROM1^UAS.cNa/Scer\GAL4^GMR.PF/Hsap\EYS^UAS.cNa

(Nie et al., 2012)

eys¹ has eye phenotype, suppressible | partially by Scer\GAL4^hs.PU/Scer\GAL4^GMR.PF/Hsap\EYS^UAS.cNa

(Nie et al., 2012)

eys¹ has rhabdomere phenotype, suppressible | partially by Scer\GAL4^hs.PU/Scer\GAL4^GMR.PF/Hsap\EYS^UAS.cNa

(Nie et al., 2012)

Enhancer of

Statement

Reference

eys[+]/eys¹ is an enhancer of ommatidium phenotype of chp[+]/chp², prom¹

(Zelhof et al., 2006)

eys[+]/eys¹ is an enhancer of rhabdomere phenotype of chp², eyc[+]/prom¹

(Zelhof et al., 2006)

eys[+]/eys¹ is an enhancer of ommatidium phenotype of chp², eyc[+]/prom¹

(Zelhof et al., 2006)

eys[+]/eys¹ is an enhancer of rhabdomere phenotype of chp[+]/chp², prom¹

(Zelhof et al., 2006)

Other

Statement

Reference

eys[+]/eys¹, prom¹ has rhabdomere of eye photoreceptor cell phenotype

(Gurudev et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype

(Nie et al., 2014)

eys[+]/eys¹, zip^HMS01618 has retina phenotype

(Nie et al., 2014)

eys¹, prom¹ has eye phenotype

(Nie et al., 2012)

eys¹, prom¹ has rhabdomere phenotype

(Nie et al., 2012)

eys¹, eyc[+]/prom¹ has ommatidium phenotype

(Zelhof et al., 2006)

eys¹, eyc[+]/prom¹ has rhabdomere phenotype

(Zelhof et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The eys¹ ommatidial phenotype is partially suppressed by crb^11A22. The number of rhabdomere clusters and individual rhabdomeres is increased, but no inter-rhabdomeral space is formed.

Flies trans-heterozygous for prom¹ and eys¹ show partial fusion of rhabdomeres. The phenotype is variable along the length of the retina.

The prom¹/+, eys¹/+ fusion phenotype is suppressed along the entire length of the rhabdomeres by heterozygous crb^11A22.

(Gurudev et al., 2014)

One copy of both eys¹ and prom¹ results in a partial failure to form a continuous open inter-rhabdomeral space (retinal lumen), exhibited by the presence of juxtaposed rhabdomeres. This phenotype is significantly enhanced by one copy of either Df(1)ED6829 or Act5C^G0245.

Expression of zip^HMS01618 under the control of Scer\GAL4^GMR.PU enhances the rhabdomere fusion seen in eys¹ prom¹ double heterozygotes.

Expression of zip^{DN.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PU enhances the rhabdomere fusion seen in eys¹ prom¹ double heterozygotes.

Expression of sqh^HMS00437 under the control of Scer\GAL4^GMR.PU enhances the rhabdomere fusion seen in eys¹ prom¹ double heterozygotes.

Expression of sqh^{E20E21.Scer\UAS.cCa} under the control of Scer\GAL4^GMR.PU and Scer\GAL4^Pph13.1.6 suppresses the rhabdomere fusion seen in eys¹ prom¹ double heterozygotes.

Expression of sna^HMS01252 under the control of Scer\GAL4^GMR.PU enhances the rhabdomere fusion seen in eys¹ prom¹ double heterozygotes.

Expression of Rho1^JF02809 under the control of Scer\GAL4^GMR.PU enhances the rhabdomere fusion seen in eys¹ prom¹ double heterozygotes.

Expression of Rok^JF03225 under the control of Scer\GAL4^GMR.PU enhances the rhabdomere fusion seen in eys¹ prom¹ double heterozygotes.

Expression of Mbs^{N300.Scer\UAS} under the control of Scer\GAL4^GMR.PU enhances the rhabdomere fusion seen in eys¹ prom¹ double heterozygotes.

Expression of zip^HMS01618 under the control of Scer\GAL4^GMR.PU in a eys¹ mutant background results in fused rhabdomeres as early as 48 hours after pupal formation.

(Nie et al., 2014)

Although the rhabdomere phenotype is recessive in eys¹ and prom¹ single mutants, eys¹/+, prom¹/+ double mutant eyes show a failure of rhabdomere separation and loss of a continuous inter-rhabdomeral space.

prom¹/prom¹; chp²/+ double mutant flies show suppression of the prom¹/prom¹ rhabdomeral phenotype. However, in prom¹/prom¹, eys¹/+; chp²/+ flies the rhabdomeres are stuck together, so the mild prom¹/prom¹; chp²/+ phenotype is enhanced in prom¹/prom¹, eys¹/+; chp²/+ flies.

(Zelhof et al., 2006)

In prom¹/prom¹, eys¹/+; chp²/+ triple mutant flies, the rescue of the prom¹/prom¹ phenotype is reduced compared to the prom¹/prom¹; chp²/+ flies, with most ommatidia in the triple mutants showing between 0 and 3 individual rhabdomeres and no ommatidia showing more than 3 individual rhabdomeres.

(Zelhof, 2006)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\EYS^Scer\UAS.cNa in the developing eye under the control of Scer\GAL4^GMR.PF and Scer\GAL4^hs.PU partially and mildly suppresses the eys¹ mutant eye phenotype, with only pockets of inter-rhabdomeral space nd many rhabdomeres remaining juxtaposed to each other.

Expression of Hsap\EYS^Scer\UAS.cNa and Hsap\PROM1^Scer\UAS.cNa in eys¹, prom¹ double mutants results in rescue of the eye phenotype, with separation of the rhabdomeres and the presence of inter-rhabdomeral space.

(Nie et al., 2012)

Complementation and Rescue Data

Partially rescued by

eys¹ is partially rescued by eys^UAS.cZa/Scer\GAL4^hs.PB

(Zelhof et al., 2006)

eys¹ is partially rescued by Scer\GAL4^ninaE.PD/eys^UAS.cZa

(Zelhof et al., 2006)

Comments

Expression of eys^Scer\UAS.cZa under the control of Scer\GAL4^hs.PB fully rescues the rhabdomere phenotype of eys¹ flies when eys^Scer\UAS.cZa expression is induced at 36-64 hours after puparium formation. Induction at about 72 hours, using either Scer\GAL4^hs.PB or Scer\GAL4^ninaE.PD, leads only to a partial rescue.

(Zelhof et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

99497

y¹ w^*; eys¹ P{neoFRT}40A cn¹ bw¹/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

eys¹

(Mahato et al., 2018, Nie et al., 2015, Gurudev et al., 2014, Nie et al., 2014, Nie et al., 2012)

spam

(Nie et al., 2012)

spam¹

(Gurudev et al., 2014, Sehadova et al., 2009, Zelhof, 2006, Zelhof et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (8)

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