FlyBase Allele Report: Dmel\prom[1]

General Information

Symbol

Dmel\prom¹

Species

D. melanogaster

Name

FlyBase ID

FBal0193674

Feature type

allele

Associated gene

Dmel\prom

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Zelhof, 2006)

Mutagen

Progenitor genotype

Cytology

Description

Lesion in the prom gene.

(Zelhof, 2008.6.16)

Nucleotide substitution: G764A.

(Zelhof, 2006)

The mutation affects the acceptor site.

(Zelhof, 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of retinal degeneration

CEA

(Gurudev et al., 2014)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Zelhof et al., 2006)

Phenotype Manifest In

eye

(Nie et al., 2012)

ommatidium

(Zelhof et al., 2006)

photoreceptor neuron | chemical conditional

(Gurudev et al., 2014)

photoreceptor neuron | conditional

(Gurudev et al., 2014)

rhabdomere

(Nie et al., 2012, Zelhof, 2006, Zelhof et al., 2006)

rhabdomere | P-stage

(Nie et al., 2015)

rhabdomere of eye photoreceptor cell

(Gurudev et al., 2014)

Detailed Description

Statement

Reference

prom¹/prom¹ mutant pupae display fusion of rhabdomeres and a reduction in size of the inter-rhabdomeral lumen.

(Nie et al., 2015)

prom¹ mutant ommatidia have fused rhabdomeres.

prom¹ mutant photoreceptor cells undergo light-dependent degeneration. The retinal degeneration can be prevented in the absence of dietary vitamin A.

(Gurudev et al., 2014)

prom¹ mutants exhibit fused rhabdomeres, with a marked loss of inter-rhabdomeral space.

(Nie et al., 2012)

prom¹ mutant adults have eyes with apparently normal microvilli. However, these eyes show a striking failure of the rhabdomeres to separate from each other, including a marked loss of inter-rhabdomeral space, irregular rhabdomere morphology and improper rhabdomeral contacts.

eyc^prom-1 mutant adults have eyes with apparently normal microvilli. However, these eyes show a striking failure of the rhabdomeres to separate from each other, including a marked loss of inter-rhabdomeral space, irregular rhabdomere morphology and improper rhabdomeral contacts.

(Zelhof et al., 2006)

In the eyes of eyc^prom-1 flies, almost all of the rhabdomeres fail to separate from one another, so that in most ommatidia only one cluster of rhabdomeres can be observed instead of 7 individual rhabdomeres.

In the eyes of prom¹ flies, almost all of the rhabdomeres fail to separate from one another, so that in most ommatidia only one cluster of rhabdomeres can be observed instead of 7 individual rhabdomeres.

(Zelhof, 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by +/Df(1)ED6829

(Nie et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by Act5C^G0245/Act5C[+]

(Nie et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by zip^HMS01618/Scer\GAL4^GMR.PU

(Nie et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by zip^DN.UAS.GFP/Scer\GAL4^GMR.PU

(Nie et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by sqh^HMS00437/Scer\GAL4^GMR.PU

(Nie et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by sna^HMS01252/Scer\GAL4^GMR.PU

(Nie et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by Rho1^JF02809/Scer\GAL4^GMR.PU

(Nie et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by Rok^JF03225/Scer\GAL4^GMR.PU

(Nie et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, enhanceable by Mbs^N300.UAS/Scer\GAL4^GMR.PU

(Nie et al., 2014)

chp[+]/chp², prom¹ has ommatidium phenotype, enhanceable by eys[+]/eys¹

(Zelhof et al., 2006)

chp², eyc[+]/prom¹ has rhabdomere phenotype, enhanceable by eys[+]/eys¹

(Zelhof et al., 2006)

chp², eyc[+]/prom¹ has ommatidium phenotype, enhanceable by eys[+]/eys¹

(Zelhof et al., 2006)

chp[+]/chp², prom¹ has rhabdomere phenotype, enhanceable by eys[+]/eys¹

(Zelhof et al., 2006)

Suppressed by

Statement

Reference

prom¹ has rhabdomere of eye photoreceptor cell phenotype, suppressible by crb^11A22

(Gurudev et al., 2014)

eys[+]/eys¹, prom¹ has rhabdomere of eye photoreceptor cell phenotype, suppressible by crb^11A22/crb[+]

(Gurudev et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype, suppressible by sqh^{E20E21.UAS.cCa}/Scer\GAL4^Pph13.1.6/Scer\GAL4^GMR.PU

(Nie et al., 2014)

prom¹ has rhabdomere phenotype, suppressible by Hsap\PROM1^UAS.cNa/Scer\GAL4^GMR.PF

(Nie et al., 2012)

prom¹ has eye phenotype, suppressible by Hsap\PROM1^UAS.cNa/Scer\GAL4^GMR.PF

(Nie et al., 2012)

prom¹ has rhabdomere phenotype, suppressible by Hsap\PROM1^ΔC.UAS.GFP/Scer\GAL4^GMR.PF

(Nie et al., 2012)

prom¹ has eye phenotype, suppressible by Hsap\PROM1^ΔC.UAS.GFP/Scer\GAL4^GMR.PF

(Nie et al., 2012)

eys¹, prom¹ has rhabdomere phenotype, suppressible by Hsap\PROM1^UAS.cNa/Scer\GAL4^GMR.PF/Hsap\EYS^UAS.cNa

(Nie et al., 2012)

eys¹, prom¹ has eye phenotype, suppressible by Hsap\PROM1^UAS.cNa/Scer\GAL4^GMR.PF/Hsap\EYS^UAS.cNa

(Nie et al., 2012)

prom¹ has rhabdomere phenotype, suppressible by chp[+]/chp²

(Zelhof et al., 2006)

prom¹ has ommatidium phenotype, suppressible by chp[+]/chp²

(Zelhof et al., 2006)

NOT suppressed by

Statement

Reference

prom¹ has rhabdomere phenotype, non-suppressible by Scer\GAL4^GMR.PF/Hsap\PROM1^mut1-7.UAS

(Nie et al., 2012)

prom¹ has eye phenotype, non-suppressible by Scer\GAL4^GMR.PF/Hsap\PROM1^mut1-7.UAS

(Nie et al., 2012)

prom¹ has rhabdomere phenotype, non-suppressible by Hsap\PROM1^R373C.UAS/Scer\GAL4^GMR.PF

(Nie et al., 2012)

prom¹ has eye phenotype, non-suppressible by Hsap\PROM1^R373C.UAS/Scer\GAL4^GMR.PF

(Nie et al., 2012)

Other

Statement

Reference

eys[+]/eys¹, prom¹ has rhabdomere of eye photoreceptor cell phenotype

(Gurudev et al., 2014)

eys¹, prom[+]/prom¹ has retina phenotype

(Nie et al., 2014)

eys¹, prom¹ has eye phenotype

(Nie et al., 2012)

eys¹, prom¹ has rhabdomere phenotype

(Nie et al., 2012)

eys¹, eyc[+]/prom¹ has ommatidium phenotype

(Zelhof et al., 2006)

eys¹, eyc[+]/prom¹ has rhabdomere phenotype

(Zelhof et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The prom¹ ommatidia phenotype is largely suppressed by crb^11A22. Most of the rhabdomeres are individualised and a single, continuous inter-rhabdomeral space is formed.

Flies trans-heterozygous for prom¹ and eys¹ show partial fusion of rhabdomeres. The phenotype is variable along the length of the retina.

The prom¹/+, eys¹/+ fusion phenotype is suppressed along the entire length of the rhabdomeres by heterozygous crb^11A22.

(Gurudev et al., 2014)

One copy of both eys¹ and prom¹ results in a partial failure to form a continuous open inter-rhabdomeral space (retinal lumen), exhibited by the presence of juxtaposed rhabdomeres. This phenotype is significantly enhanced by one copy of either Df(1)ED6829 or Act5C^G0245.

Expression of zip^HMS01618 under the control of Scer\GAL4^GMR.PU enhances the rhabdomere fusion seen in eys¹ prom¹ double heterozygotes.

Expression of zip^{DN.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PU enhances the rhabdomere fusion seen in eys¹ prom¹ double heterozygotes.

Expression of sqh^HMS00437 under the control of Scer\GAL4^GMR.PU enhances the rhabdomere fusion seen in eys¹ prom¹ double heterozygotes.

Expression of sqh^{E20E21.Scer\UAS.cCa} under the control of Scer\GAL4^GMR.PU and Scer\GAL4^Pph13.1.6 suppresses the rhabdomere fusion seen in eys¹ prom¹ double heterozygotes.

Expression of sna^HMS01252 under the control of Scer\GAL4^GMR.PU enhances the rhabdomere fusion seen in eys¹ prom¹ double heterozygotes.

Expression of Rho1^JF02809 under the control of Scer\GAL4^GMR.PU enhances the rhabdomere fusion seen in eys¹ prom¹ double heterozygotes.

Expression of Rok^JF03225 under the control of Scer\GAL4^GMR.PU enhances the rhabdomere fusion seen in eys¹ prom¹ double heterozygotes.

Expression of Mbs^{N300.Scer\UAS} under the control of Scer\GAL4^GMR.PU enhances the rhabdomere fusion seen in eys¹ prom¹ double heterozygotes.

(Nie et al., 2014)

Although the rhabdomere phenotype is recessive in eys¹ and eyc^prom-1 single mutants, eys¹/+, eyc^prom-1/+ double mutant eyes show a failure of rhabdomere separation and loss of a continuous inter-rhabdomeral space.

eyc^prom-1/eyc^prom-1; chp²/+ double mutant flies show suppression of the eyc^prom-1/eyc^prom-1 rhabdomeral phenotype. However, in eyc^prom-1/eyc^prom-1, eys¹/+; chp²/+ flies the rhabdomeres are stuck together, so the mild eyc^prom-1/eyc^prom-1; chp²/+ phenotype is enhanced in eyc^prom-1/eyc^prom-1, eys¹/+; chp²/+ flies.

Although the rhabdomere phenotype is recessive in eys¹ and prom¹ single mutants, eys¹/+, prom¹/+ double mutant eyes show a failure of rhabdomere separation and loss of a continuous inter-rhabdomeral space.

prom¹/prom¹; chp²/+ double mutant flies show suppression of the prom¹/prom¹ rhabdomeral phenotype. However, in prom¹/prom¹, eys¹/+; chp²/+ flies the rhabdomeres are stuck together, so the mild prom¹/prom¹; chp²/+ phenotype is enhanced in prom¹/prom¹, eys¹/+; chp²/+ flies.

(Zelhof et al., 2006)

In prom¹/prom¹; chp²/+ double mutant flies, the proportion of flies with one aberrant fused cluster of rhabdomeres per ommatidium is smaller than in prom¹/prom¹ single mutants. In the double mutant flies, some ommatidia show the wild-type pattern of 7 individual rhabdomeres, while most ommatidia show between 2 and 5 individual rhabdomeres, and a small proportion show one fused cluster of rhabdomeres. In prom¹/prom¹, eys¹/+; chp²/+ triple mutant flies, the rescue of the prom¹/prom¹ phenotype is reduced compared to the prom¹/prom¹; chp²/+ flies, with most ommatidia in the triple mutants showing between 0 and 3 individual rhabdomeres and no ommatidia showing more than 3 individual rhabdomeres.

In eyc^prom-1/eyc^prom-1; chp²/+ double mutant flies, the proportion of flies with one aberrant fused cluster of rhabdomeres per ommatidium is smaller than in eyc^prom-1/eyc^prom-1 single mutants. In the double mutant flies, some ommatidia show the wild-type pattern of 7 individual rhabdomeres, while most ommatidia show between 2 and 5 individual rhabdomeres, and a small proportion show one fused cluster of rhabdomeres. In eyc^prom-1/eyc^prom-1, eys¹/+; chp²/+ triple mutant flies, the rescue of the eyc^prom-1/eyc^prom-1 phenotype is reduced compared to the eyc^prom-1/eyc^prom-1; chp²/+ flies, with most ommatidia in the triple mutants showing between 0 and 3 individual rhabdomeres and no ommatidia showing more than 3 individual rhabdomeres.

(Zelhof, 2006)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\EYS^Scer\UAS.cNa and Hsap\PROM1^Scer\UAS.cNa in eys¹, prom¹ double mutants results in rescue of the eye phenotype, with separation of the rhabdomeres and the presence of inter-rhabdomeral space.

Expression of Hsap\PROM1^Scer\UAS.cNa in photoreceptor cells under the control of Scer\GAL4^Pph13 or Scer\GAL4^GMR.PF suppresses the prom¹ mutant phenotype.

Expression of Hsap\PROM1^{mut1-7.Scer\UAS} in the developing eye under the control of Scer\GAL4^GMR.PF fails to suppress the prom¹ mutant eye phenotype.

Expression of Hsap\PROM1^{ΔC.Scer\UAS.T:Avic\GFP} in the developing eye under the control of Scer\GAL4^GMR.PF suppresses the prom¹ mutant eye phenotype.

Expression of Hsap\PROM1^{R373C.Scer\UAS} in the developing eye, under the control of Scer\GAL4^GMR.PF, is not sufficient to suppress the rhabdomere phenotype in prom¹ mutants.

(Nie et al., 2012)

Complementation and Rescue Data

Fails to complement

prom²

(Zelhof, 2008.6.16)

prom^04012b

(Zelhof, 2008.6.16)

Rescued by

prom¹ is rescued by prom^UAS.cNa/Scer\GAL4^GMR.PF

(Nie et al., 2012)

prom¹ is rescued by prom^ΔC.UAS.GFP/Scer\GAL4^Pph13.1.6

(Nie et al., 2012)

Partially rescued by

prom¹ is partially rescued by prom^UAS.cZa/Scer\GAL4^hs.PB

(Zelhof, 2006)

Not rescued by

prom¹ is not rescued by Scer\GAL4^Pph13.1.6/prom^mut1-4.UAS

(Nie et al., 2012)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

FlyBase curator comment: The prom¹ allele was previously referred to as "eyc^prom-1" in FlyBase due to confusion over which transcription unit corresponds to the "eyc" complementation group. However, FBrf0198649 shows that the lesion in the mutant maps within the prom transcription unit, and thus the allele is now listed as an allele of prom in FlyBase.

(FlyBase, 1992-)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

prom¹

(Mahato et al., 2018, Nie et al., 2015, Gurudev et al., 2014, Nie et al., 2014, Nie et al., 2012, Zelhof, 2008.6.16, Zelhof, 2006, Zelhof et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (10)

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