FlyBase Allele Report: Dmel\Dscam1[unspecified]

General Information

Symbol

Dmel\Dscam1^unspecified

Species

D. melanogaster

Name

FlyBase ID

FBal0195706

Feature type

allele

Associated gene

Dmel\Dscam1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Down syndrome

modeled by Fmr1^unspecified

(Cvetkovska et al., 2013)

modeled by Fmr1^RNAi.UAS.cUa

(Cvetkovska et al., 2013)

ameliorates fragile X syndrome

modeled by Fmr1^unspecified

(Cvetkovska et al., 2013)

modeled by Fmr1^RNAi.UAS.cUa

(Cvetkovska et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult antennal lobe projection neuron DL1 adPN | somatic clone

(Zhu et al., 2006)

adult mushroom body

(Hattori et al., 2007)

antennal lobe

(Hattori et al., 2007)

antennal lobe | somatic clone

(Zhu et al., 2006)

antennal lobe glomerulus

(Hattori et al., 2007)

antennal lobe glomerulus DA1 | somatic clone

(Zhu et al., 2006)

antennal lobe glomerulus VA1v | somatic clone

(Zhu et al., 2006)

dendritic tree | somatic clone

(Zhu et al., 2006)

mushroom body

(Yu et al., 2009)

mushroom body, with Dscam1^{A-17.2-19-23.UAS.GFP}, Scer\GAL4^ey-OK107

(Yu et al., 2009)

mushroom body, with Dscam1^{A-17.2-19+23.UAS.GFP}, Scer\GAL4^ey-OK107

(Yu et al., 2009)

mushroom body, with Dscam1^{A-17.2+19-23.UAS.GFP}, Scer\GAL4^ey-OK107

(Yu et al., 2009)

mushroom body, with Dscam1^{A-17.2+19+23.UAS.GFP}, Scer\GAL4^ey-OK107

(Yu et al., 2009)

neuroblast BLV | somatic clone

(Zhu et al., 2006)

olfactory receptor neuron

(Hattori et al., 2007)

Detailed Description

Statement

Reference

Single cell MARCM clones of Dscam1^unspecified mutant mushroom body alpha/beta neurons do not effectively segregate their sister branches - multiple neurites of the same cellular origin extend into the same mushroom body lobe in 97% of clones.

Scer\GAL4^ey-OK107-mediated expression of Dscam1^{A-17.2-19-23.Scer\UAS.T:Avic\GFP} in Dscam1^unspecified clones (using the MARCM/GAL80 system) show the multibranch/lobe phenotype in 22% of cases, and a single-branch/neuron phenotype in 13% of cases.

Scer\GAL4^ey-OK107-mediated expression of Dscam1^{A-17.2-19+23.Scer\UAS.T:Avic\GFP} in Dscam1^unspecified clones (using the MARCM/GAL80 system) show the multibranch/lobe phenotype in 16% of cases, and a single-branch/neuron phenotype in 11% of cases.

Scer\GAL4^ey-OK107-mediated expression of Dscam1^{A-17.2+19-23.Scer\UAS.T:Avic\GFP} in Dscam1^unspecified clones (using the MARCM/GAL80 system) show the multibranch/lobe phenotype in 25% of cases, and a single-branch/neuron phenotype in 5% of cases.

Scer\GAL4^ey-OK107-mediated expression of Dscam1^{A-17.2+19+23.Scer\UAS.T:Avic\GFP} in Dscam1^unspecified clones (using the MARCM/GAL80 system) show the multibranch/lobe phenotype in 22% of cases, and a single-branch/neuron phenotype in 5% of cases.

(Yu et al., 2009)

Dscam null mutant homozygotes show loss of glomerular boundaries in the antennal lobe, such that axons of a given olfactory receptor neuron class target more than one glomerulus in each antennal lobe. Discrete glomeruli are still formed, but their stereotyped arrangement within the antennal lobe is highly disrupted.

Sister branches of single Dscam^unspecified mushroom body neurons in an otherwise wild-type background show a high frequency of segregation defects, often failing to segregate into the two mushroom body lobes.

(Hattori et al., 2007)

Homozygous mutant v'ada neuron clones do not have a significantly different territory size from that of control clones.

(Matthews et al., 2007)

Analysis of somatic clones does not reveal obvious mis-targeting errors in Dscam^unspecified mutant projection neurons. The dendrites of Dscam^unspecified mutant cells innervate the correct DL1 glomerulus. However, compared with controls, Dscam^unspecified mutant single cell clones for projection neurons innervating adult antennal lobe DL1 display reduced dendritic elaboration, often only partially innervating the DL1 glomerulus.

In Dscam^unspecified mutant vNb clones, the innervation of DA1 and VA1lm is strongly reduced and it is often only partial within the glomeruli.

In Dscam^unspecified mutant vNb or single-cell clones, the extent of the dendritic field is substantially reduced: dendrites only innervate the medial part of the antennal lobe. As in uni-glomerular projection neurons, the dendrites are frequently clumped.

All the wild-type single-cell clones analysed elaborate a uniform distribution of dendrites across the entire antennal lobe. By contrast, single Dscam^unspecified mutant cells do not evenly innervate all glomeruli. Dendrites in lateral regions are more affected than those in the medial region. Dscam^unspecified mutant neuroblast clones containing 5-10 local interneurons also show severe clumping defects of dedritic patterning compared with controls. A massive aggregate of dendrites form as a condensed sphere-like structure within the antennal lobe.

(Zhu et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Dscam1^unspecified is a non-enhancer of abnormal neuroanatomy | embryonic stage phenotype of mud³

(Cate et al., 2016)

Suppressor of

Statement

Reference

Dscam1^unspecified/Dscam1[+] is a suppressor of abnormal touch response phenotype of Fmr1^RNAi.UAS.cUa, Scer\GAL4^455.2

(Cvetkovska et al., 2013)

Dscam1^unspecified/Dscam1[+] is a suppressor of abnormal touch perception phenotype of Fmr1^RNAi.UAS.cUa, Scer\GAL4^455.2

(Cvetkovska et al., 2013)

Dscam1^unspecified/Dscam1[+] is a suppressor of abnormal neuroanatomy phenotype of Fmr1^unspecified

(Cvetkovska et al., 2013)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Dscam1^unspecified is a non-enhancer of larval ventral nerve cord | embryonic stage phenotype of mud³

(Cate et al., 2016)

Dscam1^unspecified is a non-enhancer of larval ventral nerve cord commissure | embryonic stage phenotype of mud³

(Cate et al., 2016)

Suppressor of

Statement

Reference

Dscam1^unspecified/Dscam1[+] is a suppressor of mechanosensory neuron phenotype of Fmr1^unspecified

(Cvetkovska et al., 2013)

Dscam1^unspecified/Dscam1[+] is a suppressor of axon | ectopic phenotype of Fmr1^unspecified

(Cvetkovska et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Dscam1^unspecified does not enhance the midline crossing defects of mud³ mutant embryos.

(Cate et al., 2016)

'Dscam[null]'/+ reduces the frequency and type of pSc axonal arbor targeting errors in 'Fmr1[null]' homozygotes.

'Dscam[null]'/+ returns the cleaning response rate of Fmr1^{dsRNA.Scer\UAS.cUa} Scer\GAL4^455.2 flies (following stimulation of the posterior scutellar bristles) back to control levels.

(Cvetkovska et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Dscam1^unspecified is rescued by Dscam1^+t73.3

(Venken et al., 2006)

Partially rescued by

Dscam1^unspecified is partially rescued by Dscam1^{A-17.2-19-23.UAS.GFP}/Scer\GAL4^ey-OK107

(Yu et al., 2009)

Dscam1^unspecified is partially rescued by Scer\GAL4^ey-OK107/Dscam1^{A-17.2-19+23.UAS.GFP}

(Yu et al., 2009)

Dscam1^unspecified is partially rescued by Scer\GAL4^ey-OK107/Dscam1^{A-17.2+19-23.UAS.GFP}

(Yu et al., 2009)

Dscam1^unspecified is partially rescued by Scer\GAL4^ey-OK107/Dscam1^{A-17.2+19+23.UAS.GFP}

(Yu et al., 2009)

Dscam1^unspecified is partially rescued by Scer\GAL4^elav-C155/Dscam1^{UAS.1.30.30.2}

(Zhan et al., 2004)

Dscam1^unspecified is partially rescued by Scer\GAL4^elav-C155/Dscam1^{UAS.11.31.25.2}

(Zhan et al., 2004)

Not rescued by

Dscam1^unspecified is not rescued by Scer\GAL4^elav-C155/Dscam1^{7.27.25.1.UAS.cZa}

(Zhan et al., 2004)

Dscam1^unspecified is not rescued by Scer\GAL4^elav-C155/Dscam1^{UAS.1.30.30.1}

(Zhan et al., 2004)

Dscam1^unspecified is not rescued by Scer\GAL4^elav-C155/Dscam1^{UAS.11.31.25.1}

(Zhan et al., 2004)

Dscam1^unspecified is not rescued by Scer\GAL4^elav-C155/Dscam1^{12.20.19.1.UAS}

(Zhan et al., 2004)

Comments

Scer\GAL4^ey-OK107-mediated expression of Dscam1^{A-17.2-19-23.Scer\UAS.T:Avic\GFP} in Dscam1^unspecified clones (using the MARCM/GAL80 system) reduces the multibranch/lobe phenotype from 97% to 22% of cases.

Scer\GAL4^ey-OK107-mediated expression of Dscam1^{A-17.2-19+23.Scer\UAS.T:Avic\GFP} in Dscam1^unspecified clones (using the MARCM/GAL80 system) reduces the multibranch/lobe phenotype from 97% to 16% of cases.

Scer\GAL4^ey-OK107-mediated expression of Dscam1^{A-17.2+19-23.Scer\UAS.T:Avic\GFP} in Dscam1^unspecified clones (using the MARCM/GAL80 system) reduces the multibranch/lobe phenotype from 97% to 25% of cases.

Scer\GAL4^ey-OK107-mediated expression of Dscam1^{A-17.2+19+23.Scer\UAS.T:Avic\GFP} in Dscam1^unspecified clones (using the MARCM/GAL80 system) reduces the multibranch/lobe phenotype from 97% to 22% of cases.

(Yu et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dscam1^unspecified

Dscam^null

(Cvetkovska et al., 2013)

Dscam^unspecified

Name Synonyms

Secondary FlyBase IDs

References (9)

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