FlyBase Allele Report: Dmel\Gug[UAS.cCa]

General Information

Symbol

Dmel\Gug^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Charroux

FlyBase ID

FBal0196227

Feature type

allele

Associated gene

Dmel\Gug

Associated Insertion(s)

Carried in Construct

P{UAS-Atro.C}

Also Known As

UAS-Atro, Atro wt

Key Links

Transgenic product class

wild_type

(Charroux et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Charroux et al., 2006)

Mutagen

in vitro construct

(Charroux et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Atro.C}

Cytology

Description

UASt regulatory sequences drive expression of the entire open reading frame of Gug.

(Charroux et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Gug

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

autophagosome, with Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

eye, with Scer\GAL4^ninaE.PT

(Nisoli et al., 2010)

eye photoreceptor cell, with Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

eye photoreceptor cell, with Scer\GAL4^ninaE.PT

(Nisoli et al., 2010)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Charroux et al., 2006)

mitochondrion, with Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

ommatidium, with Scer\GAL4^GMR.PF

(Charroux et al., 2006)

retina | temperature conditional, with Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

tarsal segment, with Scer\GAL4^dpp.blk1

(Charroux et al., 2006)

wing, with Scer\GAL4^C-580

(Charroux et al., 2006)

wing vein, with Scer\GAL4^C-580

(Charroux et al., 2006)

Detailed Description

Statement

Reference

Expression of Gug^Scer\UAS.cCa in the adult eye under the control of Scer\GAL4^ninaE.PT results in mild degeneration over time, characterised by loss of photoreceptor cells.

14 day old eyes from animals expressing Gug^Scer\UAS.cCa under the control of Scer\GAL4^GMR.PU display large autophagolysosomes in the degenerating photoreceptors. Many mitochondria show abnormal morphology, although they are hardly present in strongly degenerated tissue.

Expression of Gug^Scer\UAS.cCa in the adult eye under the control of Scer\GAL4^GMR.PU, aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue.

(Nisoli et al., 2010)

Expression of Gug^Scer\UAS.cCa in prospective wing vein tissue, driven by Scer\GAL4^C-580, leads to a substantial loss of wing veins and a reduction in wing size.

Expression of Gug^Scer\UAS.cCa under the control of Scer\GAL4^dpp.blk1 causes fusion of the tarsus in the legs.

Expression of Gug^Scer\UAS.cCa under the control of Scer\GAL4^GMR.PF causes a rough eye phenotype.

(Charroux et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Gug^UAS.cCa, Scer\GAL4^ey.PH is a non-suppressor of visible phenotype of Scer\GAL4^ey.PH, mir-8^SC1

(Vallejo et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Gug^UAS.cCa, Scer\GAL4^GMR.PU has retina | temperature conditional phenotype, enhanceable by Atg1^GS10797, Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

Gug^UAS.cCa, Scer\GAL4^GMR.PU has retina | temperature conditional phenotype, enhanceable by Atg5^RNAi.UAS, Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

Gug^UAS.cCa, Scer\GAL4^GMR.PU has retina | temperature conditional phenotype, enhanceable by mTor^TED.UAS, Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

Gug^UAS.cCa, Scer\GAL4^GMR.PF has interommatidial bristle phenotype, enhanceable by aop^UAS.cRa, Scer\GAL4^GMR.PF

(Charroux et al., 2006)

Gug^UAS.cCa, Scer\GAL4^GMR.PF has ommatidium phenotype, enhanceable by aop^UAS.cRa, Scer\GAL4^GMR.PF

(Charroux et al., 2006)

Gug^UAS.cCa, Scer\GAL4^GMR.PF has interommatidial bristle phenotype, enhanceable by pnt¹²⁷⁷/pnt^Δ88

(Charroux et al., 2006)

Gug^UAS.cCa, Scer\GAL4^GMR.PF has ommatidium phenotype, enhanceable by pnt¹²⁷⁷/pnt⁰⁷⁸²⁵

(Charroux et al., 2006)

Enhancer of

Statement

Reference

Gug^UAS.cCa, Scer\GAL4^GMR.PF is an enhancer of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, aop^UAS.cRa

(Charroux et al., 2006)

Gug^UAS.cCa, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, aop^UAS.cRa

(Charroux et al., 2006)

Gug^UAS.cCa/Scer\GAL4^GMR.PF is an enhancer of interommatidial bristle phenotype of aos^GMR.PC

(Charroux et al., 2006)

Gug^UAS.cCa/Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of aos^GMR.PC

(Charroux et al., 2006)

Suppressor of

Statement

Reference

Gug^UAS.cCa/aop^UAS.cRa, Scer\GAL4^GMR.PF is a suppressor of wing vein | increased number phenotype of Scer\GAL4^GMR.PF, btl::Egfr^λ.UAS

(Charroux et al., 2006)

Gug^UAS.cCa/Scer\GAL4^GMR.PF is a suppressor of interommatidial bristle phenotype of rho^hs.sev

(Charroux et al., 2006)

Gug^UAS.cCa/Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of rho^hs.sev

(Charroux et al., 2006)

NOT Suppressor of

Statement

Reference

Gug^UAS.cCa, Scer\GAL4^ey.PH is a non-suppressor of eye ventral compartment phenotype of Scer\GAL4^ey.PH, mir-8^SC1

(Vallejo et al., 2011)

Gug^UAS.cCa/Scer\GAL4^GMR.PU is a non-suppressor of eye | somatic clone phenotype of Atg1^Δ3D

(Nisoli et al., 2010)

Gug^UAS.cCa, Scer\GAL4^GMR.PF is a non-suppressor of wing vein | increased number phenotype of Scer\GAL4^GMR.PF, btl::Egfr^λ.UAS

(Charroux et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Gug^Scer\UAS.cCa does not suppress the loss of ventral eye tissue caused by expression of mir-8^SC1 under the control of Scer\GAL4^ey.PH.

(Vallejo et al., 2011)

Expression of Gug^Scer\UAS.cCa under the control of Scer\GAL4^GMR.PU in Atg1^Δ3D mutant eyes fails to rescue cell degeneration.

Expression of Gug^Scer\UAS.cCa in the adult eye under the control of Scer\GAL4^GMR.PU, aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is enhanced by co-expression of Atg5^{dsRNA.Scer\UAS}.

(Nisoli et al., 2010)

Expression of Gug^Scer\UAS.cCa, under the control of Scer\GAL4^GMR.PF, suppresses the rough eye phenotype of rho^hs.sev-expressing flies.

Expression of Gug^Scer\UAS.cCa, under the control of Scer\GAL4^GMR.PF, enhances the rough eye phenotype seen in flies that express argos^GMR.PC.

Flies that coexpress Gug^Scer\UAS.cCa and aop^Scer\UAS.cRa, under the control of Scer\GAL4^GMR.PF, show an enhanced rough eye phenotype, compared to flies expressing either single transgene. The third instar discs of flies expressing both transgenes are normal, suggesting that the effects observed in the adult eyes occur during the pupal stage.

The rough eye phenotype of flies expressing Gug^Scer\UAS.cCa under the control of Scer\GAL4^GMR.PF is enhanced in both an pnt¹²⁷⁷/pnt^Δ88 and an pnt¹²⁷⁷/pnt⁰⁷⁸²⁵ background.

Expression of either Gug^Scer\UAS.cCa or aop^Scer\UAS.cRa has very little effect on the extra wing vein material produced in flies that overexpress btl::Egfr^{Scer\UAS.T:λ\cI-DD} under the control of Scer\GAL4^GMR.PF. However, this wing vein phenotype is suppressed when Gug^Scer\UAS.cCa and aop^Scer\UAS.cRa are both expressed along with btl::Egfr^{Scer\UAS.T:λ\cI-DD} (all under the control of Scer\GAL4^GMR.PF).

(Charroux et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Gug^UAS.cCa/Scer\GAL4^dpp.blk1 rescues Gug^RNAi.1.UAS

(Wang et al., 2008)

Gug^UAS.cCa/Scer\GAL4^dpp.blk1 rescues Gug^RNAi.2.UAS

(Wang et al., 2008)

Gug^UAS.cCa/Scer\GAL4^arm.PS rescues Gug³⁵

(Charroux et al., 2006)

Gug^UAS.cCa/Scer\GAL4^arm.PS rescues Gug⁰³⁹²⁸

(Charroux et al., 2006)

Comments

Expression of Gug^{dsRNA.1.Scer\UAS} in the L3 and L4 inter-vein region under the control of Scer\GAL4^dpp.blk1 results in ectopic wing vein formation, which is rescued by co-expression of Gug^Scer\UAS.cCa.

Expression of Gug^{dsRNA.2.Scer\UAS} in the L3 and L4 inter-vein region under the control of Scer\GAL4^dpp.blk1 results in ectopic wing vein formation, which is rescued by co-expression of Gug^Scer\UAS.cCa.

(Wang et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Gug^Scer\UAS.cCa

Gug^UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Charroux

Secondary FlyBase IDs

References (6)

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