FB2024_03 , released June 25, 2024
Allele: Dmel\Tctph59
Open Close
General Information
Symbol
Dmel\Tctph59
Species
D. melanogaster
Name
FlyBase ID
FBal0197208
Feature type
allele
Associated gene
Dmel\Tctp
Associated Insertion(s)
Carried in Construct
Also Known As
dTCTPh59
Key Links
Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Hsu et al., 2007)
Mutagen
P-element activity
(Hsu et al., 2007)
Progenitor genotype
TctpEY09182
(Hsu et al., 2007)
Cytology
Description

Scer\UAS regulatory sequences drive expression of the full-length Tctp coding region and 5' Kozak sequence.

(Hsu et al., 2007)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Tctph59 germline mosaic clones display loss of germline stem cells in adult testes.

      (Liu et al., 2016)

      TctpEY09182/Tctph59 flies show higher sensitivity to gamma irradiation compared to controls. Mitotic chromosomes from both larval brain neuroblasts and adult wing hair cells show more than twofold higher levels of double-strand break (DSB) repair defects after gamma irradiation in TctpEY09182/Tctph59 animals compared to controls.

      G2/M arrest after gamma irradiation is much reduced in TctpEY09182/Tctph59 mutants compared to controls, with the difference being most pronounced at a low dose of radiation (5 Gy). The intra-S checkpoint (as assayed by BrdU incorporation in the larval brain after irradiation) is reduced in TctpEY09182/Tctph59 mutants compared to wild type.

      DSBs induced by radiation result in an approximately two-fold increase cell death in TctpEY09182/Tctph59 mutants compared to controls at 5, 10 and 20 Gy of irradiation.

      (Hong and Choi, 2013)

      Homozygous clones have a growth disadvantage compared to their wild-type twin spots; homozygous clones induced in the larval imaginal disc are similar in size to their twin spots 24 hours after induction, much reduced in size compared to their twin spots 48 hours after induction and most homozygous clones are eliminated by 60 hours after induction.

      Mosaic flies in which the eye tissue is mostly homozygous for Tctph59 (generated using the EGUF/Hid technique) have either small eyes or no eyes.

      (Hsu et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Tctph59 has lethal phenotype, suppressible | partially by Scer\GAL4Act.PU/Hsap\TPT1UAS.cHa

      (Hsu et al., 2007)
      Other
      Statement
      Reference

      Tctph59/Tctp[+], tefuatm-3 has abnormal developmental rate | dominant phenotype

      (Hong and Choi, 2013)
      Phenotype Manifest In
      Suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      tefuatm-3/Tctph59 double heterozygotes show a 2 day delay in the start of pupation compared to either wild-type controls or either single heterozygote.

      (Hong and Choi, 2013)

      Expression of CycEScer\UAS.cRa under the control of Scer\GAL4tub results in four times more Tctph59 cells being present in clones 72 hours after their induction.

      Animals in which the eye is doubly mutant for Tsc1Q600X and Tctph59 have a small or no-eye phenotype indistinguishable from the Tctph59 single mutant phenotype.

      (Hsu et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Hsap\TPT1Scer\UAS.cHa under the control of Scer\GAL4Act.PU rescues Tctph59 homozygotes to the pupal or adult stage.

      (Hsu et al., 2007)
      Complementation and Rescue Data
      Rescued by

      Tctph59/TctpEY09182 is rescued by Tctp+tGe

      (Hong and Choi, 2013)

      Tctph59 is rescued by Tctp+t3

      (Hsu et al., 2007)
      Partially rescued by

      Tctph59 is partially rescued by TctpUAS.cHa/Scer\GAL4Act.PU

      (Hsu et al., 2007)
      Comments

      Tctp+tGe rescues the increased sensitivity of TctpEY09182/Tctph59 flies to gamma irradiation.

      (Hong and Choi, 2013)

      Expression of TctpScer\UAS.cHa under the control of Scer\GAL4Act.PU rescues Tctph59 homozygotes to the pupal or adult stage.

      (Hsu et al., 2007)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Tctph59
      (Kim et al., 2023, Hong and Choi, 2016)
      Tctph5p
      (Liu et al., 2016)
      dTCTPh59
      (Hong and Choi, 2013, Hsu et al., 2007)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)