FB2024_03 , released June 25, 2024
Allele: Dmel\COX5BGD6659
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General Information
Symbol
Dmel\COX5BGD6659
Species
D. melanogaster
Name
FlyBase ID
FBal0199243
Feature type
allele
Associated gene
Dmel\COX5B
Associated Insertion(s)
Carried in Construct
P{GD6659}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD6659}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD6659
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cytochrome-c oxidase deficiency disease
      CEA
      (Kemppainen et al., 2014)
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal developmental rate, with Scer\GAL4arm.PU

      (Klichko et al., 2014)

      abnormal developmental rate | RU486 conditional, with Scer\GAL4da.Switch.PT

      (Rera et al., 2010)

      abnormal locomotor behavior | adult stage, with Scer\GAL4arm.PU

      (Klichko et al., 2014)

      abnormal locomotor behavior | adult stage, with Scer\GAL4elav-C155

      (Kemppainen et al., 2014)

      abnormal stress response, with Scer\GAL4Act5C.Switch.PR

      (Zid et al., 2009)

      female sterile | RU486 conditional, with Scer\GAL4da.Switch.PT

      (Rera et al., 2010)

      increased mortality during development, with Scer\GAL4arm.PU

      (Klichko et al., 2014)

      lethal, with Scer\GAL4da.PU

      (Klichko et al., 2014)

      lethal, with Scer\GAL4Tub.PU

      (Klichko et al., 2014)

      lethal | RU486 conditional, with Scer\GAL4da.Switch.PT

      (Rera et al., 2010)

      lethal - all die during larval stage, with Scer\GAL4da.PU

      (Kemppainen et al., 2014)

      lethal - all die during P-stage, with Scer\GAL4G14

      (Kemppainen et al., 2014)

      lethal - all die during P-stage, with Scer\GAL4Mef2.PR

      (Schnorrer et al., 2010)

      partially lethal - majority die, with Scer\GAL4elav-C155

      (Kemppainen et al., 2014)

      partially lethal - majority die, with Scer\GAL4pnr-MD237

      (Mummery-Widmer et al., 2009)

      partially lethal - majority die | RU486 conditional, with Scer\GAL4αTub84B.Switch.PK

      (Kemppainen et al., 2014)

      short lived, with Scer\GAL4arm.PU

      (Klichko et al., 2014)

      short lived |  :nutrition conditional | RU486 conditional, with Scer\GAL4Act5C.Switch.PR

      (Zid et al., 2009)

      short lived | female | RU486 conditional, with Scer\GAL4da.Switch.PT

      (Rera et al., 2010)

      short lived | male, with Scer\GAL4elav-C155

      (Kemppainen et al., 2014)

      short lived | male | RU486 conditional, with Scer\GAL4da.Switch.PT

      (Rera et al., 2010)

      some die during pupal stage, with Scer\GAL4pnr-MD237

      (Mummery-Widmer et al., 2009)

      viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu

      (Neely et al., 2010)

      viable, with Scer\GAL4C57

      (Kemppainen et al., 2014)

      viable, with Scer\GAL4Ddc.PL

      (Kemppainen et al., 2014)

      viable, with Scer\GAL4elav.PLu

      (Kemppainen et al., 2014)

      viable, with Scer\GAL4nrv2.PS

      (Kemppainen et al., 2014)

      viable, with Scer\GAL4smid-C161

      (Kemppainen et al., 2014)

      viable, with Scer\GAL4tin.CΔ4

      (Neely et al., 2010)

      viable, with Scer\GAL4VGlut-OK371

      (Kemppainen et al., 2014)

      visible, with Scer\GAL4pnr-MD237

      (Mummery-Widmer et al., 2009)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of COX5BGD6659 under the control of Scer\GAL4ey.PU induces disruption to the patterning of ommatidia.

      (Choo et al., 2015)

      Expression of COX5BGD6659 driven by Scer\GAL4αTub84B.Switch.PK results in dosage-dependent lethality: 0.5uM RU486 results in zero eclosing flies, with survival increasing as dosage is decreased to 0.1uM. Scer\GAL4αTub84B.Switch.PK>COX5BGD6659 flies on 3uM RU486 fail to develop beyond the larval stage. Expression of COX5BGD6659 driven by Scer\GAL4da.PU leads to larval lethality. Expression of COX5BGD6659 driven by Scer\GAL4smid-C161, Scer\GAL4Ddc.PL, Scer\GAL4nrv2.PS, Scer\GAL4elav.PLu, Scer\GAL4C57 or Scer\GAL4VGlut-OK371 does not lead to lethality; progeny are also not bang sensitive. Expression of COX5BGD6659 mainly in thoracic muscles driven by Scer\GAL4G14 produces lethality during the late pupal stage or during attempted eclosion.

      Expression of COX5BGD6659 driven by Scer\GAL4elav-C155 (no effect seen with Scer\GAL4elav.PLu) leads to almost complete lethality, with males more affected than heterozygous females; surviving flies also show locomotor impairments (more severe in males) and males are short lived.

      (Kemppainen et al., 2014)

      Expression of COX5BGD6659 under the control of either Scer\GAL4da.PU or Scer\GAL4tub.PU leads to lethality.

      (Klichko et al., 2014)

      Adults expressing COX5BGD6659 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)

      Expression of CG11015GD6659 under the control of Scer\GAL4da.Switch.PT in the presence of 20μ/ml RU486 results in lethality.

      Expression of CG11015GD6659 under the control of Scer\GAL4da.Switch.PT in the presence of 1μ/ml RU486 results in a prolonged developmental phase compared to controls.

      Expression of CG11015GD6659 under the control of Scer\GAL4da.Switch.PT in the presence of 1μ/ml RU486 results in a significant decrease in female fertility.

      Expression of CG11015GD6659 under the control of Scer\GAL4da.Switch.PT in the presence of 1μ/ml RU486 does not result in a significant change in body weight in male flies.

      Expression of CG11015GD6659 under the control of Scer\GAL4da.Switch.PT in the presence of 1μ/ml RU486 significantly reduces adult lifespan compared to controls in both males and females.

      (Rera et al., 2010)

      Expression under the control of Scer\GAL4Mef2.PR results in early pupal lethality.

      (Schnorrer et al., 2010)

      Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 20-30% of the Scer\GAL4pnr-MD237 expression domain.

      Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 80-90% of the Scer\GAL4pnr-MD237-expressing area of the notum.

      (Mummery-Widmer et al., 2009)

      Under rich nutritional conditions the lifespan of CG11015GD6659 knockdown flies (under the control of Scer\GAL4Act5C.Switch.PR and RU486) is indistinguishable from control flies. However, upon dietary restriction, knockdown diminishes lifespan extension in both male and female flies compared to their respective controls.

      (Zid et al., 2009)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      NOT suppressed by
      Other
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      COX5BGD6659, Scer\GAL4ey.PU has eye phenotype, enhanceable by WwoxKK108459, Scer\GAL4ey.PU

      (Choo et al., 2015)
      Suppressed by
      Statement
      Reference

      COX5BGD6659, Scer\GAL4ey.PU has eye phenotype, suppressible | partially by WwoxUAS.cCa, Scer\GAL4ey.PU

      (Choo et al., 2015)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Zzzz\AOXαTub84B.PK significantly partially suppresses dosage-dependent lethality (with 0.1 to 0.5uM RU486) in flies with expression of COX5BGD6659 driven by Scer\GAL4αTub84B.Switch.PK; further suppression is seen with two copies of Zzzz\AOXαTub84B.PK. Scer\GAL4αTub84B.Switch.PK>COX5BGD6659 flies with 4 copies of Zzzz\AOXαTub84B.PK on 3uM RU486 reach pupal stage (with 12% of pupae eclosing); these flies are not bang sensitive. Co-expression of Zzzz\AOXScer\UAS.cFa (but not Scer\NDI1Scer\UAS.cSa) enables development to proceed to the pupal stage (and a few flies eclose) in flies with expression of COX5BGD6659 driven by Scer\GAL4da.PU. Co-expression of Zzzz\AOXScer\UAS.cFa rescues pupal lethality in flies with expression of COX5BGD6659 driven by Scer\GAL4G14, however flies rescued in this way show severe locomotor defects and die within 2 weeks as a result of becoming trapped in the food; multiple copies of Zzzz\AOXαTub84B.PK also partially rescue lethality.

      Co-expression of Zzzz\AOXScer\UAS.cFa (but not Scer\NDI1Scer\UAS.cSa) partially rescues lethality in flies with expression of COX5BGD6659 driven by Scer\GAL4elav-C155. Co-expression of Zzzz\AOXScer\UAS.cFa partially rescues shortened lifespan in surviving males with expression of COX5BGD6659 driven by Scer\GAL4elav-C155; whereas co-expression of Scer\NDI1Scer\UAS.cSa leads to a shortened lifespan in females and further enhances the shortened lifespan in males.

      (Kemppainen et al., 2014)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      CG11015GD6659
      CG11015v30892
      (Rera et al., 2010)
      COX5BGD6659
      CoVbGD6659
      CoVbRNAi
      (Choo et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (12)