FB2024_03 , released June 25, 2024
Allele: Dmel\ScoxGD898
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General Information
Symbol
Dmel\ScoxGD898
Species
D. melanogaster
Name
FlyBase ID
FBal0206030
Feature type
allele
Associated gene
Dmel\Scox
Associated Insertion(s)
Carried in Construct
P{GD898}
Also Known As
scox KD
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD898}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD898
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Ubiquitous knockdown of ScoxGD898 driven by Scer\GAL4da.PU causes developmental arrest at the early third instar larval stage and larvae are smaller than wild type.

      Flies with cardiac-specific knockdown of ScoxGD898 driven by Scer\GAL4tin.CΔ4 have a shorter lifespan (begin to die after 2 weeks) than controls, and show heart dysfunction (aggravated with age and dose-dependent). With one or two copies of ScoxGD898, diastolic interval is significantly increased and fractional shortening (heart tube contractility) is significantly decreased in both 1 or 2 week old flies, compared to controls; two copies significantly increases heart period (reduces heart rate) in 1 or 2 week old flies (one copy significantly increases heart period in 2 week old flies); two copies (but not one) significantly decreases both diastolic and systolic diameter in 1 or 2 week old flies. Cardiac-specific knockdown of ScoxGD898 (two copies) also leads to myofibrillar disorganization (including gaps between myofibrils, disorganization is strongest in the posterior half) and a narrowing of the heart tube (in 1 week old, and even more so 2 week old flies); conical chamber diameter is also significantly increased in 1 week old flies, compared to controls. Pupal heart structure appears normal. Scer\GAL4tin.CΔ4>ScoxGD898 hearts show increases in reactive oxygen species production; they also show signs of apoptosis (seen at 1 week and more so at 2 weeks old).

      (Martínez-Morentin et al., 2015)

      No apparent difference in morphology is observed with larvae and adults expressing Scer\GAL4Act.PU>ScoxGD898. However, the expression of ScoxGD898 is associated with lethality at the larval stage. The transgenic larvae survive for 12 days after hatching, but demonstrate reduced motility day by until death. The larvae stay within the medium with low mobility or no climbing of the walls of culture tubes, in clear contrast to wild-type flies.

      (Nguyen et al., 2014)

      Expression of ScoxGD898 under the control of Scer\GAL4pnr-MD237 results in a mild thoracic cleft, a reduction in the scutellum and thin bristles, typical of "Minute" mutations.

      Expression of ScoxGD898 under the control of Scer\GAL4GMR.PFa has no effect on adult eye morphology.

      (Binks et al., 2010)

      Adults expressing ScoxGD898 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)

      Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in early pupal lethality or late pupal lethality.

      (Schnorrer et al., 2010)

      Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 100% of the Scer\GAL4pnr-MD237 expression domain.

      (Mummery-Widmer et al., 2009)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4pnr-MD237, ScoxGD898 has visible phenotype, non-suppressible by Ctr1AdN.UAS.Tag:FLAG, Scer\GAL4pnr-MD237

      (Binks et al., 2010)
      NOT Suppressor of
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      NOT Suppressor of
      Statement
      Reference

      ScoxGD898, Scer\GAL4pnr-MD237 is a non-suppressor of adult cuticle phenotype of ATP7UAS.Tag:FLAG, Scer\GAL4pnr-MD237

      (Binks et al., 2010)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Flies with co-expression of p53Scer\UAS.cUa along with Scer\GAL4tin.CΔ4>ScoxGD898 have no heartbeat and show significant heart degeneration, with complete loss of cardiac myofibrils.

      Co-expression of rprScer\UAS.cUa increases myofibril disorganization seen in adult hearts of Scer\GAL4tin.CΔ4>ScoxGD898 flies (defects in 1 week old flies look similar to 2 week old Scer\GAL4tin.CΔ4>ScoxGD898 flies).

      Co-expression of p53DN.Scer\UAS.cUa suppresses heart function and structure defects seen in Scer\GAL4tin.CΔ4>ScoxGD898 flies: diastolic interval, heart period and fractional shortening return to control levels and there are no obvious morphological heart defects (including correct alignment of cardiomyocyte myofibrils). Presence of p535A-1-4/p535A-1-4 suppresses heart structural defects seen in Scer\GAL4tin.CΔ4>ScoxGD898 flies: there are no obvious morphological heart defects (including correct alignment of cardiomyocyte myofibrils).

      Co-expression of Diap1Scer\UAS.cUa rescues heart structural defects seen in Scer\GAL4tin.CΔ4>ScoxGD898 flies.

      (Martínez-Morentin et al., 2015)

      Co-expression of Ctr1AScer\UAS.T:Zzzz\FLAG has no effect on the "Minute"-like phenotype caused by expression of ScoxGD898 under the control of Scer\GAL4pnr-MD237.

      Co-expression of ScoxGD898 does not suppress the cuticle hypopigmentation phenotype caused by expression of ATP7Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4pnr-MD237.

      (Binks et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of BacA\p35Scer\UAS.cUa rescues heart structural defects seen in Scer\GAL4tin.CΔ4>ScoxGD898 flies.

      (Martínez-Morentin et al., 2015)
      Complementation and Rescue Data
      Rescued by

      ScoxGD898 is rescued by ScoxUAS.Tag:FLAG/Scer\GAL4pnr-MD237

      (Binks et al., 2010)
      Comments

      Co-expression of ScoxScer\UAS.T:Zzzz\FLAG rescues the "Minute"-like defects caused by expression of ScoxGD898 under the control of Scer\GAL4pnr-MD237.

      (Binks et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CG8885GD898
      ScoxGD898
      Name Synonyms
      Secondary FlyBase IDs
        References (10)