Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ may result in mild defects in the shape of ganglion mother cells in the larval brain (depending on the insertion line used).

Scer\GAL4^da.G32>JhI-1^GD10927 embryos are completely viable. They do not show any patterning defects and they hatch into larvae at the same time and size as wild-type control animals. Initially, the knockdown larvae show a healthy development and by the end of the second day AED (after egg deposition) they are able to molt successfully into the second instar. However, from the very beginning, knockdown larvae grow more slowly than the control and after the first molt almost stop gaining weight. They survive to another 3-4 days only reaching about a 15-fold increase in mass over newly hatched larvae. The control animals develop normally reaching about 200-fold increase in mass by the end of larval development and pupariate 5-6 days AED. At approximately the same time, a small portion of the Scer\GAL4^da.G32>JhI-1^GD10927 larvae die as second instars (as demonstrated by the morphology of the mouth hooks), but most knockdown animals manage to initiate the transition to third instar and die during molting either with both second and third instar mouth hooks attached or with a partially shed cuticle. Practically the same phenotype is observed for with another ubiquitous driver, Scer\GAL4^αTub84B.PL.

Scer\GAL4^hs.PB>JhI-1^GD10927 animals are completely viable, though upon pupariation, knockdown larvae are unable to adhere themselves tightly to the wall of the vial. Salivary glands dissected from knockdown white prepupae are significantly smaller than those from wild-type control animals of the same age, and rather have the size of the glands from the second instar. There is no significant difference in the total number of cells in salivary glands from knockdown larvae as compared to controls. However, Scer\GAL4^hs.PB>JhI-1^GD10927 causes a decrease in cell size, nuclear size and DNA content. Although knockdown nuclei fail to attain normal size, they are noticeably bigger and packed tighter than control nuclei of the second instar. At around 72 hours AED, the nuclei of Scer\GAL4^hs.PB>JhI-1^GD10927 knockdown salivary glands do not show any growth, but do undergo an additional round of endoreplication reaching ploidy of 64C. During second instar (72 hours AED), endoreplication is practically normal in Scer\GAL4^hs.PB>JhI-1^GD10927 animals. During third instar, knockdown cells show a significant reduction in DNA synthesis, although several endocycling nuclei are still detected.

Males and females expressing JhI-1^GD10927 under the control of Scer\GAL4^A9 appear normal. The number of knockdown females is significantly smaller than expected. About 67% of them die as late pupae or pharate adults. The wing blades of female escapers are bent upward suggesting that the dorsal surface of the wing is reduced.

All the animals expressing JhI-1^GD10927 under the control of Scer\GAL4^ap-md544 dies during metamorphosis. Dissected Scer\GAL4^ap-md544>JhI-1^GD10927 pharate adults reveal a whole range of dramatic phenotypes in the thoracic segment: tiny wing blades, absent scutellum, and split notum.

About 76% of animals expressing JhI-1^GD10927 under the control of Scer\GAL4^ey.PH die as pharate adults. The surviving 24% display a range of phenotypes in one or both eyes, such as small eye, split eye, or no eye. Knockdown animals with small eyes display a significant reduction of the ommatidial number and a severe disruption of the regular ommatidial array, compared with wild-type. The mean size of the ommatidia is not significantly reduced in knockdowns, indicating that cell size is not affected by expression of JhI-1^GD10927 in mitotic cells. When animals exhibiting pupal lethality are dissected from their puparia, the pharate adults reveal a headless phenotype, missing all or most of the structures derived from eye-antennal imaginal discs, resulting in reduced head capsules.

Expression of JhI-1^GD10927 in the predominantly postmitotic cells posterior to the morphogenetic furrow of the developing eye under the control of Scer\GAL4^GMR.PF does not reduce viability. The eyes of flies expressing JhI-1^GD10927 under the control of Scer\GAL4^GMR.PF do not show any eye abnormalities. Even 30 days post eclosion, eye size and ommatidial integrity appear similar in knockdown and control flies.

Adults expressing JhI-1^GD10927 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD10927} insertion line used.

Adults expressing JhI-1^GD10927 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show no significant change in the kinetics of high temperature (46[o]C) induced paralysis compared to control flies.

Expression under the control of Scer\GAL4^Mef2.PR results in early pupal lethality.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 40-50% or 90-100% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 10-20% or 60-70% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 50-60% of the Scer\GAL4^pnr-MD237-expressing area of the notum.