FB2024_03 , released June 25, 2024
Allele: Dmel\NGD144
Open Close
General Information
Symbol
Dmel\NGD144
Species
D. melanogaster
Name
FlyBase ID
FBal0207691
Feature type
allele
Associated gene
Dmel\N
Associated Insertion(s)
Carried in Construct
P{GD144}
Also Known As
Notch-IR, UAS-Notch RNAi, UAS-Notch-RNAi, N-IR
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD144}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD144
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  alcohol use disorder
      CEA
      (Petruccelli et al., 2018)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of NGD144 under the control of Scer\GAL4en-e16E leads to increased cell death in third instar larval wing discs.

      (Sun et al., 2020)

      The clonal expression of NGD144 under the control of Scer\GAL4Act.PU leads to several polar/stalk cell defects compared to controls: decreased number of polar/stalk cells; stalk cells do not show the normal intercalated morphology; and high frequency of fused egg chambers.

      (Dai et al., 2017)

      Expression of NGD144 under the control of Scer\GAL4ple.PU as well as the Scer\GAL4elav.PU driver in combination with Scer\GAL80ple.PK (all together with tub-Gal80[ts] to limit the time of expression to adulthood) results in significantly decreased adult lifespan.

      (Imai et al., 2015)

      Expression of NGD144 under the control of Scer\GAL4insc-Mz1407 results in the loss of type II neuroblasts.

      (Lee et al., 2013)

      Expression of NGD144 under the control of Scer\GAL4esg-NP7397 in the adult intestine (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit expression) at 22[o]C results in a mild increase in the density of both enteroendocrine cells and enterocytes, although the ratio of these two differentiated cell types is normal. At 25 and 28[o]C, there is a dramatic increase in the number of intestinal stem cell-like cells, as well as an increase in enteroendocrine cells and concomitant loss of enterocytes.

      (Perdigoto et al., 2011)

      NGD144 driven by Scer\GAL4sev.PU leads to significant cone cell loss in third instar larvae eye discs, but no defects are observed in the photoreceptors.

      Adult flies expressing NGD144 driven by Scer\GAL4sev.PU have glossy retinas and the eyes contain black patches of tissues indicating necrosis.

      (Cordero and Cagan, 2010)

      Adults expressing NGD144 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)

      Expression under the control of Scer\GAL4Mef2.PR results in late pupal lethality.

      (Schnorrer et al., 2010)

      Expression under the control of Scer\GAL4pnr-MD237 results in extra bristles on the notum in 10-20% of the Scer\GAL4pnr-MD237 expression domain.

      Expression under the control of Scer\GAL4pnr-MD237 results in loss of bristles on the notum in 90-100% of the Scer\GAL4pnr-MD237 expression domain.

      (Mummery-Widmer et al., 2009)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Phenotype Manifest In
      Suppressed by
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The co-expression of NGD144 (together with Dicer-2 transgene, for efficient RNAi) fully suppresses the increased number of type II neuroblasts in third instar larval brains resulting from the co-expression of lilliScer\UAS.SR.T:Zzzz\FLAG and Su(Tpl)Scer\UAS.SR.T:Hsap\MYC under the control of Scer\GAL4insc-Mz1407, even to the point of near absence of type II neuroblasts, as compared to controls; a similar suppression is not achieved when the phenotype arises from the expression of E(spl)mγ-HLHScer\UAS.cLb alone.

      (Liu et al., 2017)

      Co-expression of either MarfmiRNA.cUa.Scer\UAS or dmScer\UAS.cZa partially rescues the loss of type II neuroblasts seen in animals expressing NGD144 under the control of Scer\GAL4insc-Mz1407, while simultaneous co-expression of both MarfmiRNA.cUa.Scer\UAS and dmScer\UAS.cZa together fully rescues the phenotype.

      (Lee et al., 2013)

      wgl-17/+ suppresses the necrosis seen in adult eyes when NGD144 is driven by Scer\GAL4sev.PU.

      wgl-17/+ suppresses the cone cell loss phenotype seen in the third instar eye discs when NGD144 is driven by Scer\GAL4sev.PU.

      pygoS123/+ suppresses the necrosis seen in adult eyes when NGD144 is driven by Scer\GAL4sev.PU.

      pygoS123/+ suppresses the cone cell loss seen in the third instar eye discs when NGD144 is driven by Scer\GAL4sev.PU.

      (Cordero and Cagan, 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      NGD144
      NotchKD
      (Dai et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (22)