FlyBase Allele Report: Dmel\bsk[GD10555]

General Information

Symbol

Dmel\bsk^GD10555

Species

D. melanogaster

Name

FlyBase ID

FBal0209184

Feature type

allele

Associated gene

Dmel\bsk

Associated Insertion(s)

Carried in Construct

P{GD10555}

Also Known As

UAS-bsk^RNAi, UAS-Bsk RNAi, bsk^RNAi, UAS-JNK-RNAi, bsk RNAi, bsk-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10555}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10555

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates colorectal cancer

modeled by SH3PX1^JF02730

(Zhang et al., 2019)

exacerbates Huntington's disease

modeled by Hsap\HTT^{Q91.ex1.QUAS.mCherry}

(Pearce et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ey-OK107

(Rallis et al., 2010)

abnormal planar polarity, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

abnormal stress response, with Scer\GAL4^da.G32

(Hull-Thompson et al., 2009)

abnormal stress response, with Scer\GAL4^Ilp2.PR

(Karpac et al., 2009)

abnormal stress response | RU486 conditional, with Scer\GAL4^{Ilp2.Switch.PK}

(Karpac et al., 2009)

chemical resistant, with Scer\GAL4^ple.PF

(Ortega-Arellano et al., 2013)

decreased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Biteau et al., 2010)

decreased occurrence of cell division | adult stage, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Biteau et al., 2010)

decreased occurrence of cell division | adult stage | RU486 conditional, with Scer\GAL4^bun-GSG5961

(Biteau et al., 2010)

long lived | RU486 conditional, with Scer\GAL4^bun-GSG5961

(Biteau et al., 2010)

short lived, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Biteau et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

adult gut, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Biteau et al., 2010)

adult gut | RU486 conditional, with Scer\GAL4^bun-GSG5961

(Biteau et al., 2010)

axon, with Scer\GAL4^ey-OK107

(Rallis et al., 2010)

intestinal stem cell | adult stage, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Biteau et al., 2010)

Kenyon cell, with Scer\GAL4^ey-OK107

(Rallis et al., 2010)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

midgut | adult stage, with Scer\GAL4^Act.PU

(Wang et al., 2014)

Detailed Description

Statement

Reference

Expressing bsk^GD10555 under the control of Scer\GAL4^C587 (together with Dicer-2, for a more efficient RNAi) does not induce any obvious L3 spermatogenesis defects.

(La Marca et al., 2019)

The expression of bsk^GD10555 under the control of Scer\GAL4^GMR.long does not induce any eye phenotype (overall morphology, ommatidial array, interommatidial bristles), as compared to controls; eye discs do not show any apoptosis.

(Yan et al., 2019)

Expression of bsk^GD10555 under the control of Scer\GAL4^Su(H).GBE (using tub-gal80[ts] to limit the time of expression) does not significantly affect cell proliferation in the adult gut.

(Wang et al., 2015)

6 days of expression of bsk^GD10555 in clones under the combined control of Scer\GAL4^Act.PU and Gal80[ts] leads to a decrease in the proportion of mitotic cells in the adult midgut, as compared to control clones.

(Wang et al., 2014)

Expression of bsk^GD10555 in the posterior compartment of the imaginal wing disc, under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU does not affect levels of cell death.

(Wong et al., 2014)

Expression of bsk^GD10555 under the control of Scer\GAL4^ple.PF does not lead to a significant difference in survival or climbing capability as compared to controls under normal conditions; however, these flies exhibit increased resistance to paraquat toxicity, with a significant increase in survival and climbing performance when exposed to paraquat, as compared to control flies exposed to the same treatment.

(Ortega-Arellano et al., 2013)

Transient expression of bsk^GD10555 in adults under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B strongly inhibits proliferation of intestinal stem cells compared with controls, and prevents tissue regeneration.

Transient expression of bsk^GD10555 in adults under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B results in significant lifespan shortening compared with wild-type.

Compared with controls, female flies expressing bsk^GD10555 under the control of Scer\GAL4^bun-GSG5961 show a moderate, but significant, reduction in intestinal cell proliferation at old age. These flies are significantly longer lived when exposed to RU486 than isogenic siblings exposed to mock treatment.

(Biteau et al., 2010)

Adults expressing bsk^GD10555 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression of bsk^GD10555 using Scer\GAL4^ey-OK107 results in axon defasciculation, axon degeneration and axon overextension phenotypes. These phenotypes are not as frequent if bsk^GD10555 expression is restricted to shorter periods of development (using the TARGET system).

(Rallis et al., 2010)

Flies expressing bsk^GD10555 under the control of Scer\GAL4^da.G32 more sensitive to dry starvation than wild-type controls.

(Hull-Thompson et al., 2009)

Flies expressing bsk^GD10555 under the control of Scer\GAL4^Ilp2.PR show a significantly reduced repression of growth (assayed by measuring wing area) when shifted from 25[o]C to 29[o]C compared to wild-type flies.

Flies expressing bsk^GD10555 under the control of Scer\GAL4^Ilp2.PR are significantly more sensitive to paraquat than control flies.

Flies expressing bsk^GD10555 under the control of Scer\GAL4^{Ilp2.Switch.PK} show increased sensitivity to paraquat compared to controls in the presence of RU486.

(Karpac et al., 2009)

Expression under the control of Scer\GAL4^pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 40-50% or 50-60% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in mild or strong thorax closure defects, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in an enlarged notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10555

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ey-OK107, bsk^GD10555 has abnormal neuroanatomy phenotype, enhanceable by Scer\GAL4^ey-OK107/kay^Fbz.UAS

(Rallis et al., 2010)

Scer\GAL4^ey-OK107, bsk^GD10555 has abnormal neuroanatomy phenotype, enhanceable by Jra^Jbz.UAS, Scer\GAL4^ey-OK107

(Rallis et al., 2010)

Enhancer of

Statement

Reference

Scer\GAL4^da.PU/bsk^GD10555 is an enhancer of abnormal cell cycle phenotype of Scer\GAL4^da.PU, brat^HMS01121

(Hussain et al., 2017)

Suppressor of

Statement

Reference

Scer\GAL4^C587/bsk^GD10555 is a suppressor | partially of abnormal size | recessive | male | third instar larval stage phenotype of Strip^jam

(La Marca et al., 2019)

bsk^GD10555, Scer\GAL4^C587 is a suppressor | partially of abnormal size | male | third instar larval stage phenotype of Cka^GD12225, Scer\GAL4^C587

(La Marca et al., 2019)

bsk^GD10555, Scer\GAL4^GMR.long is a suppressor | partially of visible | adult stage phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

bsk^GD10555, Scer\GAL4^GMR.long is a suppressor | partially of increased cell death | third instar larval stage phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

bsk^GD10555, Scer\GAL4^esg.PU is a suppressor | partially of increased occurrence of cell division | adult stage | progressive phenotype of SH3PX1^HMJ21552, Scer\GAL4^esg.PU

(Zhang et al., 2019)

bsk^GD10555, Scer\GAL4^elav.PU is a suppressor of increased cell death | pupal stage phenotype of Myd88^UAS.cMa, Scer\GAL4^elav.PU

(Foldi et al., 2017)

bsk^GD10555, Scer\GAL4^elav.PU is a suppressor of increased cell death | pupal stage phenotype of Sarm^EP3610, Scer\GAL4^elav.PU

(Foldi et al., 2017)

Scer\GAL4^da.PU/bsk^GD10555 is a suppressor of abnormal size phenotype of Scer\GAL4^da.PU, brat^HMS01121

(Hussain et al., 2017)

bsk^GD10555, Scer\GAL4^cv-c-C5 is a suppressor | partially of abnormal size | adult stage phenotype of Dad1^GD1006, Scer\GAL4^cv-c-C5

(Zhang et al., 2016)

bsk^GD10555, Scer\GAL4^cv-c-C5 is a suppressor | partially of visible | adult stage phenotype of Dad1^GD1006, Scer\GAL4^cv-c-C5

(Zhang et al., 2016)

bsk^GD10555, Scer\GAL4^en.PU is a suppressor of increased cell death | third instar larval stage phenotype of Dad1^GD1006, Scer\GAL4^en.PU

(Zhang et al., 2016)

bsk^GD10555/Scer\GAL4^en.PU is a suppressor of increased cell number | third instar larval stage phenotype of Scer\GAL4^en.PU, vtd^GL00522

(Liu et al., 2015)

bsk^GD10555/Scer\GAL4^en.PU is a suppressor of increased cell death | third instar larval stage phenotype of Scer\GAL4^en.PU, vtd^GL00522

(Liu et al., 2015)

Scer\GAL4^Su(H).GBE/bsk^GD10555 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^Su(H).GBE, Xbp1^GD4745

(Wang et al., 2015)

bsk^GD10555/Scer\GAL4^Bx-MS1096 is a suppressor of increased cell death phenotype of Scer\GAL4^Bx-MS1096, mrn^GD4089, p53^Ct.GUS

(Villicaña et al., 2013)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.PU/bsk^GD10555 is a non-suppressor of neoplasia phenotype of Scer\GAL4^da.PU, brat^HMS01121

(Hussain et al., 2017)

bsk^GD10555, Scer\GAL4^en.PU is a non-suppressor of increased occurrence of cell division | third instar larval stage phenotype of Dad1^GD1006, Scer\GAL4^en.PU

(Zhang et al., 2016)

Other

Statement

Reference

Scer\GAL4^hh.PU, bsk^GD10555, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^GD10555, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Dark^VDRC.cUa, Scer\GAL4^hh.PU, bsk^GD10555, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Dark^VDRC.cUa, Scer\GAL4^en.PU, bsk^GD10555, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ey-OK107, bsk^GD10555 has Kenyon cell phenotype, enhanceable by Scer\GAL4^ey-OK107/kay^Fbz.UAS

(Rallis et al., 2010)

Scer\GAL4^ey-OK107, bsk^GD10555 has axon phenotype, enhanceable by Scer\GAL4^ey-OK107/kay^Fbz.UAS

(Rallis et al., 2010)

Scer\GAL4^ey-OK107, bsk^GD10555 has Kenyon cell phenotype, enhanceable by Jra^Jbz.UAS, Scer\GAL4^ey-OK107

(Rallis et al., 2010)

Scer\GAL4^ey-OK107, bsk^GD10555 has axon phenotype, enhanceable by Jra^Jbz.UAS, Scer\GAL4^ey-OK107

(Rallis et al., 2010)

Suppressed by

Statement

Reference

Scer\GAL4^Act.PU, bsk^GD10555 has midgut | somatic clone | adult stage phenotype, suppressible | somatic clone by Scer\GAL4^Act.PU/Xbp1^GD4745

(Wang et al., 2014)

Suppressor of

Statement

Reference

Scer\GAL4^C587/bsk^GD10555 is a suppressor | partially of gonad | male | third instar larval stage phenotype of Strip^jam

(La Marca et al., 2019)

Scer\GAL4^C587/bsk^GD10555 is a suppressor | partially of male germline cyst | third instar larval stage phenotype of Strip^jam

(La Marca et al., 2019)

Scer\GAL4^C587/bsk^GD10555 is a suppressor | partially of spermatogonium | third instar larval stage phenotype of Strip^jam

(La Marca et al., 2019)

Scer\GAL4^C587/bsk^GD10555 is a suppressor | partially of spermatocyte | third instar larval stage phenotype of Strip^jam

(La Marca et al., 2019)

Scer\GAL4^C587/bsk^GD10555 is a suppressor | partially of cyst cell of testis | third instar larval stage phenotype of Strip^jam

(La Marca et al., 2019)

bsk^GD10555, Scer\GAL4^C587 is a suppressor | partially of gonad | male | third instar larval stage phenotype of Cka^GD12225, Scer\GAL4^C587

(La Marca et al., 2019)

bsk^GD10555, Scer\GAL4^C587 is a suppressor | partially of male germline stem cell | third instar larval stage phenotype of Cka^GD12225, Scer\GAL4^C587

(La Marca et al., 2019)

bsk^GD10555, Scer\GAL4^C587 is a suppressor | partially of spermatogonium | third instar larval stage phenotype of Cka^GD12225, Scer\GAL4^C587

(La Marca et al., 2019)

bsk^GD10555, Scer\GAL4^C587 is a suppressor | partially of male germline cyst | third instar larval stage phenotype of Cka^GD12225, Scer\GAL4^C587

(La Marca et al., 2019)

bsk^GD10555, Scer\GAL4^C587 is a suppressor | partially of cyst cell of testis | third instar larval stage phenotype of Cka^GD12225, Scer\GAL4^C587

(La Marca et al., 2019)

bsk^GD10555, Scer\GAL4^GMR.long is a suppressor | partially of eye phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

bsk^GD10555, Scer\GAL4^GMR.long is a suppressor | partially of ommatidium phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

bsk^GD10555, Scer\GAL4^GMR.long is a suppressor | partially of interommatidial bristle phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

bsk^GD10555, Scer\GAL4^GMR.long is a suppressor | partially of eye disc phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

bsk^GD10555, Scer\GAL4^elav.PU is a suppressor of larval ventral nerve cord | pupal stage phenotype of Myd88^UAS.cMa, Scer\GAL4^elav.PU

(Foldi et al., 2017)

bsk^GD10555, Scer\GAL4^elav.PU is a suppressor of larval ventral nerve cord | pupal stage phenotype of Sarm^EP3610, Scer\GAL4^elav.PU

(Foldi et al., 2017)

bsk^GD10555, Scer\GAL4^cv-c-C5 is a suppressor | partially of wing phenotype of Dad1^GD1006, Scer\GAL4^cv-c-C5

(Zhang et al., 2016)

bsk^GD10555, Scer\GAL4^en.PU is a suppressor of wing disc | third instar larval stage phenotype of Dad1^GD1006, Scer\GAL4^en.PU

(Zhang et al., 2016)

bsk^GD10555/Scer\GAL4^en.PU is a suppressor of wing disc posterior compartment | third instar larval stage phenotype of Scer\GAL4^en.PU, vtd^GL00522

(Liu et al., 2015)

bsk^GD10555/Scer\GAL4^en.PU is a suppressor of wing disc | third instar larval stage phenotype of Scer\GAL4^en.PU, vtd^GL00522

(Liu et al., 2015)

bsk^GD10555/Scer\GAL4^en.PU is a suppressor of hemocyte | third instar larval stage phenotype of Scer\GAL4^en.PU, vtd^GL00522

(Liu et al., 2015)

Scer\GAL4^Su(H).GBE/bsk^GD10555 is a suppressor of adult gut phenotype of Scer\GAL4^Su(H).GBE, Xbp1^GD4745

(Wang et al., 2015)

bsk^GD10555/Scer\GAL4^NP5130 is a suppressor | somatic clone of midgut | somatic clone | adult stage phenotype of Scer\GAL4^NP5130, Xbp1^GD4745

(Wang et al., 2014)

NOT Suppressor of

Statement

Reference

bsk^GD10555, Scer\GAL4^en.PU is a non-suppressor of wing disc | third instar larval stage phenotype of Dad1^GD1006, Scer\GAL4^en.PU

(Zhang et al., 2016)

Other

Statement

Reference

Scer\GAL4^da.PU, brat^HMS01121, bsk^GD10555 has embryonic/larval brain phenotype

(Hussain et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The increased cell death (number of apoptotic Dcp-1-positive cells) in the ventral nerve cord of pupae expressing either Ect4^EP3610 or Myd88^Scer\UAS.cMa under the control of Scer\GAL4^elav.PU can be suppressed by co-expression of bsk^GD10555.

(Foldi et al., 2017)

The larval brain overgrowth exhibited upon expression of brat^HMS01121 under the control of Scer\GAL4^da.PU is suppressed when bsk^GD10555 is co-expressed. The proportion of defective anaphases seen in larval brains expressing brat^HMS01121 under the control of Scer\GAL4^da.PU is significantly elevated upon co-expression of bsk^GD10555, but the level of cell death in these brains does not significantly change. The tumor growth rate of larval brain explants expressing brat^HMS01121 under the control of Scer\GAL4^da.PU is not suppressed upon co-expression bsk^GD10555.

(Hussain et al., 2017)

The small wing size induced by Scer\GAL4^C5-controlled expression of Dad1^GD1006 is partially suppressed by the co-expression of bsk^GD10555. Co-expression of bsk^GD10555 suppresses the apoptosis, but not the compensatory cell division, induced by Scer\GAL4^en.PU-controlled expression of Dad1^GD1006 in third instar larval wing discs.

(Zhang et al., 2016)

Visual system knockdown of bsk, through expression of bsk^GD10555 under the control of Scer\GAL4^ey.PH in a sicily^E or Marf^B mutant background significantly reduces lipid droplet accumulation.

(Liu et al., 2015)

The expression of vtd^GL00522 under the control of Scer\GAL4^en.PU (together with Dicer-2, for efficient RNAi) leads to a significant increase in apoptosis in the posterior compartment of the third instar larval wing disc, which is greatly suppressed by co-expressing bsk^GD10555.

Expression of vtd^GL00522 under the control of Scer\GAL4^en.PU (together with Dicer-2, for efficient RNAi) results in increased numbers of hemocytes on the surface of third instar larval wing discs, as compared to controls, which are suppressed by co-expressing bsk^GD10555.

Expression of vtd^GL00522 under the control of Scer\GAL4^en.PU (together with Dicer-2, for efficient RNAi) increases the level of reactive oxygen species in the posterior compartment of the third instar larval wing disc, as compared to controls, which are not decreased by co-expressing bsk^GD10555.

(Liu et al., 2015)

The increased cell proliferation in the adult gut characteristic for flies expressing Xbp1^GD4745 under the control of Scer\GAL4^Su(H).GBE (using tub-Gal80[ts] to limit the time of expression) is suppressed by co-expression of bsk^GD10555.

(Wang et al., 2015)

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when bsk^GD10555 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. This leads to an increase in cell death.

Knockdown of Dark, through expression of Dark^VDRC.cUa results in significantly reduced levels of cell death in imaginal wing disc cells expressing bsk^GD10555 and mad2^GD16427.

(Wong et al., 2014)

The massive apoptosis seen in wing discs co-expressing p53^Ct.GUS and mrn^GD4089 under the control of Scer\GAL4^Bx-MS1096 is practically abolished by co-expression of bsk^GD10555.

(Villicaña et al., 2013)

Co-expression of either one copy of kay^Fbz.Scer\UAS or two copies of Jra^Jbz.Scer\UAS strongly enhances the axonal defects seen in Scer\GAL4^ey-OK107, bsk^GD10555 animals.

(Rallis et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v34138

w¹¹¹⁸; P{GD10555}v34138

v34139

w¹¹¹⁸; P{GD10555}v34139/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10555

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

JNK^RNAi

(Chen et al., 2020)

bsk^GD10555

bsk^RNAi2

(Pearce et al., 2015)

bsk^RNAi3

(Pearce et al., 2015)

bsk^RNAi

(Wang et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (35)

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