FB2024_03 , released June 25, 2024
Allele: Dmel\psidin2
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General Information
Symbol
Dmel\psidin2
Species
D. melanogaster
Name
FlyBase ID
FBal0212218
Feature type
allele
Associated gene
Dmel\psidin
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Brennan et al., 2007)
Mutagen
ethyl methanesulfonate
(Brennan et al., 2007)
Progenitor genotype
Cytology
Description

Amino acid replacement: Q160term.

(Brennan et al., 2007)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:20,023,548..20,023,548 [+]
Nucleotide change:

C20023548T

Amino acid change:

Q160term | psidin-PA

Reported amino acid change:

Q160term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Brennan et al., 2007)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Ten to twenty percent of psidin2/Df(3R)H15 blood cells are enlarged.

      psidin2/Df(3R)H15 mutant blood cells engulf E.coli in a manner indistinguishable from that of wild-type. However, psidin2/Df(3R)H15 mutant blood cells fail to digest them. In some cases, the bacteria appear to have replicated in the phagosomes. At 4hrs post infection, in 50 wild-type cells, only a single bacterium is visible. In contrast, 12 of 50 psidin2/Df(3R)H15 mutant blood cells contain a single bacterium, and four contain two or more intracellular E.coli. Counts of colony-forming units show that wild-type animals clear an infection of 12,000 units within 5hrs, whereas mutant animals exhibit indistinguishable numbers of bacteria at 0 and 5hr after infection. The maintenance of stable numbers of bacteria suggest that the bacterial replication observed within some blood cells may be balanced by some bacterial killing.

      (Brennan et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      psidin2
      (Brennan, 2007)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)