Unlike controls, cry⁰¹ individuals maintain circadian rhythmicity under constant light.

I-LNv neurons in cry⁰¹ individuals show a severely impaired electrophysiological response to light stimulus, as compared to controls (i.e. Scer\GAL4^cry.PZ>cry^UAS.EGFP, cry⁰¹ rescued individuals): UV and blue light stimulation induces membrane potential hyperpolarization during/after stimulation instead of the expected depolarization, and induces a much lower firing frequency after stimulation, although the response duration is unaffected; red light stimulus fails to induce the expected membrane potential depolarization during/after stimulation, but does not change the firing frequency after stimulation.

When cry⁰¹ individuals are rescued by Scer\GAL4^cry.PZ>cry^{W420Y.UAS.EGFP}, I-LNv neurons show membrane potential depolarization to UV, blue and red light stimulation, but of much lower amplitude than controls; there is a much lower firing frequency after UV and blue light stimulation, dispute of a similar response duration, as compared to controls. These individuals predominantly arrhythmic under moderate-low constant light, as controls, but not under very low light.

When cry⁰¹ individuals are rescued by Scer\GAL4^cry.PZ>cry^{W397Y.UAS.EGFP}, I-LNv neurons show lower membrane potential depolarization to UV light stimulation than controls (i.e. Scer\GAL4^cry.PZ>cry^UAS.EGFP, cry⁰¹ rescued individuals), and unaffected lower attenuated duration of response, despite of unaffected firing frequency, as compared to controls; the electrophysiological responses to blue and red light are unaffected as compared to controls. These individuals predominantly arhythmic under moderate-low or very low constant light, as controls.

cry⁰¹ homozygous adults reared under 12h:12h white light:dark cycles exhibit a significantly earlier morning activity onset and significantly later evening activity onset, as compared to controls. These mutants show an apparently normal resynchronization to a 6h delay in 12h:12h white light:dark cycle, as well as no obvious rhythmicity defects after a few days of either constant light or darkness, as compared to controls.

cry⁰¹ homozygous adults previously trained under 12h:12h light:dark cycles present a subtle decrease in circadian rhythmicity under constant darkness conditions, fail to become arrhythmic under constant light conditions, present a severe decrease in circadian phase shifting in response to light pulses during the night period, present a significant delay in circadian phase shifting in response to a 8h delay in the light:dark transition, and present defective arousal - i.e. a significant decrease in the frequency of arousal and a significant delay in arousal - in response to a light pulse during the night period, as compared to controls; the large ventrolateral neurons (l-LNv) of the adult brain in these mutants exhibit a severe decrease in the frequency of firing in response to white light, which is less severe in response to blue light (405nm) than to orange light (550-1000nm), as compared to controls.

cry⁰¹ mutants exhibit normal light-dependent temperature preference. Similarly to wild-type, they prefer higher temperature in the light than in the dark.

cry⁰¹ mutant class IV dendritic arborization neurons do not exhibit any defects in light response.

Homozygous cry⁰¹ flies do not show a naive preference to a magnetic field, or an enhanced preference to a magnetic field after training, under full-spectrum light conditions. cry⁰¹/Canton-S flies do show a significant response under the same conditions.

Transheterozygous cry^b/cry⁰¹ flies do not show significant naive or trained responses to a magnetic field.

Homozygous flies show robust periodic locomotion in two free-running conditions; constant darkness (DD) and constant light (LL) (a condition that causes genetically normal flies to go arrhythmic). In DD, most cry⁰¹ flies show solidly rhythmic locomotion at 25^o or 29^oC. Period values are generally in the normal range, except that approximately 20% of the mutant flies tested at 25^oC give shorter than normal values. At 18^oC, cry⁰¹ flies show weak rhythmicity, although the rhythmic individuals have normal period values. In LL, most cry⁰¹ flies behave rhythmically at 25^oC. Most of the rhythmic individuals have two rhythmic components within a given behavioural record in LL, the average for the relatively short free-running period is 21-22 hours and that of the longer is 25-26 hours. At 29^oC and at 18^oC in LL, cry⁰¹ flies show a relatively high degree of arrhythmicity. At 25^oC in LL, when periodic components can be reliably analysed, approximately 60% of the flies show two periodicities whose average values are 20 and 24 hours. At 18^oC in LL, the rhythmic mutant flies show only one periodic component, of approximately 24 hours.

Most cry⁰¹/Df(3R)Dl-BX12 flies behave rhythmically under constant light conditions at 25^oC, approximately one-third of the mutant flies have one periodic component (approximately 24 hours) and the remainder have two periodic components per individual (approximately 22 and 25 hours).

cry⁰¹ flies entrained to 12 hours light: 12 hour dark cycles and then exposed to 8 hour delayed light-dark (LD) cycles are able to resynchronise to the new light regime.

cry⁰¹ flies that are entrained to 12 hour light: 12 hour dark cycles ( 12:12 LD) and are then shifted to 9:9 , 13:13 or 14:14 LD cycles routinely re-entrain to behave in synchrony with the novel non-24 hour cycles.

cry⁰¹ has abnormal locomotor rhythm | adult stage phenotype, enhanceable by Rh7⁰/Rh7⁰

cry⁰¹ has abnormal circadian rhythm | adult stage phenotype, enhanceable by norpA^P24/norpA^P24

cry⁰¹ has photoperiod response variant | adult stage phenotype, enhanceable by Rh7¹/Rh7¹

cry⁰¹ has abnormal locomotor rhythm | adult stage phenotype, suppressible by Rh7⁰/Rh7⁰

cry⁰¹ has abnormal circadian rhythm | adult stage phenotype, suppressible by Rh5²/Rh5²/Rh6¹/Rh6¹

cry⁰¹, norpA^P24 has abnormal circadian rhythm | adult stage phenotype, suppressible by trp^MB03672/trp^MB03672

cry⁰¹, norpA^P24 has abnormal circadian rhythm | adult stage phenotype, suppressible by trpl^MB10553/trpl^MB10553

cry⁰¹/cry⁰¹ is an enhancer of abnormal locomotor rhythm | adult stage phenotype of Rh7⁰

cry⁰¹/cry⁰¹ is an enhancer of photoperiod response variant | adult stage phenotype of Rh7¹

cry⁰¹, norpA^P41 has abnormal circadian behavior | adult stage phenotype

Rh7¹, cry⁰¹ has abnormal circadian rhythm | adult stage phenotype

cry⁰¹, norpA^P24 has abnormal locomotor rhythm phenotype