FlyBase Allele Report: Dmel\ATP7[UAS.Tag:FLAG]

General Information

Symbol

Dmel\ATP7^UAS.Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0221296

Feature type

allele

Associated gene

Dmel\ATP7

Associated Insertion(s)

Carried in Construct

P{UAS-ATP7.FLAG}

Also Known As

UAS-ATP7^FLAG, UAS-ATP7

Key Links

Transgenic product class

cDNA

(Norgate et al., 2006)

wild_type

(Norgate et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Norgate et al., 2006)

wild_type

(Norgate et al., 2006)

Mutagen

in vitro construct

(Norgate et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-ATP7.FLAG}

Cytology

Description

Expression of ATP7 cDNA (amplified from S2 cell cDNA with primers: F1 5' gaGGTACCatgtccacggtgcgcctgcc 3' and R1 5' gcTCTAGAcagcttttgcagttcggtAct 3'), fused in frame at the c-terminus to the epitope flag Tag:FLAG, is under the control of UASt sequences.

(Norgate et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ATP7

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Menkes disease

CEA

(Hwang et al., 2014)

model of metal metabolism disorder

CEA

(Hartwig et al., 2021, Zlatic et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Menkes disease

modeled by ATP7^ΔP17

(Norgate et al., 2006)

model of metal metabolism disorder

is ameliorated by Uch^GD11265

(Zlatic et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^Act5C.PI

(Norgate et al., 2006)

abnormal body color, with Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016, Southon et al., 2008, Norgate et al., 2006)

abnormal body color | adult stage, with Scer\GAL4^pnr-MD237

(Binks et al., 2010)

chemical sensitive, with Scer\GAL4^elav-C155

(Hwang et al., 2014)

chemical sensitive, with Scer\GAL4^mex1.2.1

(Southon et al., 2013)

lethal | chemical conditional, with Scer\GAL4^mex1.2.1

(Zhang et al., 2020)

lethal | pupal stage, with Scer\GAL4^tracheal

(Norgate et al., 2006)

partially lethal - majority die | third instar larval stage, with Scer\GAL4^tracheal

(Norgate et al., 2006)

partially lethal - majority live, with Scer\GAL4^elav-C155

(Hwang et al., 2014)

visible, with Scer\GAL4^elav-C155

(Hwang et al., 2014)

visible, with Scer\GAL4^elav-C155, Scer\GAL80^CCAP.PL

(Hwang et al., 2014)

visible, with Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

visible | adult stage, with Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Phenotype Manifest In

adult abdomen, with Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

adult cuticle, with Scer\GAL4^pnr-MD237

(Binks et al., 2010)

adult thoracic sensillum, with Scer\GAL4^pnr-MD237

(Southon et al., 2008)

adult thorax, with Scer\GAL4^pnr-MD237

(Zhang et al., 2020, Mercer and Burke, 2016, Southon et al., 2008)

macrochaeta & mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Norgate et al., 2006)

macrochaeta | absent, with Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Norgate et al., 2006)

microchaeta | absent, with Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

scutellar bristle, with Scer\GAL4^ptc-559.1

(Norgate et al., 2006)

scutellum, with Scer\GAL4^pnr-MD237

(Norgate et al., 2006)

scutellum, with Scer\GAL4^ptc-559.1

(Norgate et al., 2006)

tergite, with Scer\GAL4^Act5C.PI

(Norgate et al., 2006)

trident, with Scer\GAL4^pnr-MD237

(Norgate et al., 2006)

wing, with Scer\GAL4^elav-C155

(Hwang et al., 2014)

wing, with Scer\GAL4^elav-C155, Scer\GAL80^CCAP.PL

(Hwang et al., 2014)

Detailed Description

Statement

Reference

Expression of ATP7^UAS.Tag:FLAG under the control of Scer\GAL4^pnr-MD237 leads to loss of pigmentation on the abdomen, loss of sensory micro and macrochaetae, and a thoracic cleft when compared to control adults.

Expression of ATP7^UAS.Tag:FLAG under the control of Scer\GAL4^mex1.2.1 leads to an increase in survival on normal food but results in decreased survival on CuCl2-supplemented food when compared to controls.

(Zhang et al., 2020)

Expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} driven in the thoracic midline by Scer\GAL4^pnr-MD237 causes a mild thoracic cleft and hypo-pigmentation of the thorax and abdomen, compared to controls.

(Mercer and Burke, 2016)

Expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^elav-C155 does not significantly affect larval lethality compared to controls. Scer\GAL4^CCAP.PP>ATP7^{Scer\UAS.T:Zzzz\FLAG} third instar larval brains do not show a significant decrease in the density of axon branching or number of cell bodies or area of neurons compared to controls.

(Mercer et al., 2016)

Expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav-C155 results in reduced survival from first instar larval stage to adulthood, as compared with controls, with most fatalities occurring at the pharate adult stage, and a subset of adults display an unexpanded wing phenotype and tanning defects; but does not lead to any delay in developmental time. Lethality and the unexpanded wing phenotype are both enhanced as compared to controls upon dietary supplementation with the copper chelator bathocuproine disulfonate at 300uM. The expanded wing phenotype is not significantly ameliorated by the addition of a Scer\GAL80^CCAP.PL construct to inhibit expression in the CCAP cells.

Expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^CCAP.PP does not cause any significant increase in the number of adults with unexpanded wings, as compared to controls.

(Hwang et al., 2014)

Animals expressing ATP7^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^mex1.2.1 are sensitive to dietary copper compared to controls.

(Southon et al., 2013)

Expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^pnr-MD237 results in a hypopigmentation phenotype in the adult dorsal cuticle of the thorax and abdomen (in the domain of Scer\GAL4 expression).

Expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PFa has no effect on the eye.

(Binks et al., 2010)

Expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^pnr-MD237 causes hypopigmentation and loss of sensory bristles in the pnr region, and reduced thoracic width in adult flies.

(Southon et al., 2008)

ATP7^{Scer\UAS.T:Zzzz\FLAG}; Scer\GAL4^Act5C.PI grown on copper deficient medium have reduced abdominal pigmentation. This phenotype is largely rescued by adding back copper to the medium, but not by supplementing the copper deficient medium with iron or zinc. ATP7^{Scer\UAS.T:Zzzz\FLAG}; Scer\GAL4^pnr-MD237 adults have a non pigmented stripe running down the midline of the notum. They also have a cleft in the thorax, reduction in the scutellum and variable loss of thoracic bristles. This loss of pigmentation is independent of dietary copper levels.

ATP7^{Scer\UAS.T:Zzzz\FLAG}; Scer\GAL4^tracheal animals die as late larvae or early pupae.

ATP7^{Scer\UAS.T:Zzzz\FLAG}; Scer\GAL4^ptc-559.1 adults show reduction of the scutellum and partial or complete loss of scutellar bristles.

(Norgate et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, enhanceable by Mvl^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237

(Southon et al., 2008)

NOT Enhanced by

Statement

Reference

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has visible | adult stage phenotype, non-enhanceable by slmb^UAS.cGa, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, non-enhanceable by Mvl^97f/Mvl[+]

(Southon et al., 2008)

Suppressed by

Statement

Reference

ATP7^UAS.Tag:FLAG, Scer\GAL4^mex1.2.1 has chemical sensitive phenotype, suppressible by Psn^KK109329, Scer\GAL4^mex1.2.1

(Southon et al., 2013)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has abnormal body color | adult stage phenotype, suppressible by Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237

(Binks et al., 2010)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has abnormal body color | adult stage phenotype, suppressible by Ctr1B^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237

(Binks et al., 2010)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, suppressible by Ctr1A^{dN.UAS.Tag:FLAG}

(Norgate et al., 2006)

NOT suppressed by

Statement

Reference

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has visible | adult stage phenotype, non-suppressible by slmb^UAS.cGa, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has abnormal body color | adult stage phenotype, non-suppressible by Scox^GD898, Scer\GAL4^pnr-MD237

(Binks et al., 2010)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, non-suppressible by Mvl^97f/Mvl[+]

(Southon et al., 2008)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, non-suppressible by Ccs^UAS.Tag:FLAG

(Norgate et al., 2006)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, non-suppressible by Atox1^UAS.Tag:FLAG

(Norgate et al., 2006)

Enhancer of

Statement

Reference

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 is an enhancer of visible phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

ATP7^UAS.Tag:FLAG, Scer\GAL4^elav-C155 is an enhancer of majority die during larval stage phenotype of Gclc^GD9767, Scer\GAL4^elav-C155

(Mercer et al., 2016)

ATP7^UAS.Tag:FLAG, Scer\GAL4^CCAP.PP is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Gclc^GD9767, Scer\GAL4^CCAP.PP

(Mercer et al., 2016)

ATP7^UAS.Tag:FLAG, Scer\GAL4^CCAP.PP is an enhancer of decreased cell size | third instar larval stage phenotype of Gclc^GD9767, Scer\GAL4^CCAP.PP

(Mercer et al., 2016)

Suppressor of

Statement

Reference

ATP7^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa is a suppressor of visible phenotype of Ctr1B^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa

(Binks et al., 2010)

NOT Suppressor of

Statement

Reference

ATP7^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa is a non-suppressor of visible phenotype of Ctr1A^GD16726, Scer\GAL4^GMR.PFa

(Binks et al., 2010)

Other

Statement

Reference

ATP7^UAS.Tag:FLAG, Ctr1A^GD16726, Scer\GAL4^CCAP.PP has visible phenotype

(Hwang et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has adult thorax phenotype, enhanceable by Mvl^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237

(Southon et al., 2008)

NOT Enhanced by

Statement

Reference

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has adult thorax phenotype, non-enhanceable by slmb^UAS.cGa, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has macrochaeta | absent phenotype, non-enhanceable by slmb^UAS.cGa, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has microchaeta | absent phenotype, non-enhanceable by slmb^UAS.cGa, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has adult thorax phenotype, non-enhanceable by Mvl^97f/Mvl[+]

(Southon et al., 2008)

Suppressed by

Statement

Reference

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has adult cuticle phenotype, suppressible by Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237

(Binks et al., 2010)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has adult cuticle phenotype, suppressible by Ctr1B^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237

(Binks et al., 2010)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has trident phenotype, suppressible by Ctr1A^{dN.UAS.Tag:FLAG}

(Norgate et al., 2006)

NOT suppressed by

Statement

Reference

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has macrochaeta | absent phenotype, non-suppressible by slmb^UAS.cGa, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has microchaeta | absent phenotype, non-suppressible by slmb^UAS.cGa, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has adult thorax phenotype, non-suppressible by slmb^UAS.cGa, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has adult cuticle phenotype, non-suppressible by Scox^GD898, Scer\GAL4^pnr-MD237

(Binks et al., 2010)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 has adult thorax phenotype, non-suppressible by Mvl^97f/Mvl[+]

(Southon et al., 2008)

Enhancer of

Statement

Reference

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 is an enhancer of adult thorax phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 is an enhancer of scutellum phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

ATP7^UAS.Tag:FLAG, Scer\GAL4^CCAP.PP is an enhancer of embryonic/larval brain | third instar larval stage phenotype of Gclc^GD9767, Scer\GAL4^CCAP.PP

(Mercer et al., 2016)

ATP7^UAS.Tag:FLAG, Scer\GAL4^CCAP.PP is an enhancer of axon | third instar larval stage phenotype of Gclc^GD9767, Scer\GAL4^CCAP.PP

(Mercer et al., 2016)

Suppressor of

Statement

Reference

ATP7^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa is a suppressor of eye phenotype of Ctr1B^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa

(Binks et al., 2010)

NOT Suppressor of

Statement

Reference

ATP7^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa is a non-suppressor of eye phenotype of Ctr1A^GD16726, Scer\GAL4^GMR.PFa

(Binks et al., 2010)

Other

Statement

Reference

ATP7^UAS.Tag:FLAG, Ctr1A^GD16726, Scer\GAL4^CCAP.PP has wing phenotype

(Hwang et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} enhances the thoracic cleft and scutellum loss seen in flies with expression of Grx1^GD10587 driven by Scer\GAL4^pnr-MD237 (compared to expression of either alone).

(Mercer and Burke, 2016)

Co-expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} significantly enhances larval lethality seen with expression of Gclc^GD9767 driven by Scer\GAL4^elav-C155. Co-expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} significantly exacerbates the decrease of axon branching and Scer\GAL4^CCAP.PP neuron area in third instar larval brains with expression of Gclc^GD9767 driven by Scer\GAL4^CCAP.PP.

(Mercer et al., 2016)

Co-expression of Ctr1A^GD16726 and ATP7^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^CCAP.PP causes a highly penetrant unexpanded wing phenotype, as compared to controls.

(Hwang et al., 2014)

Co-expression of Psn^KK109329 suppresses the copper sensitivity seen in animals expressing ATP7^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^mex1.2.1.

(Southon et al., 2013)

Co-expression of either Ctr1A^{Scer\UAS.T:Zzzz\FLAG} or Ctr1B^{Scer\UAS.T:Zzzz\FLAG} rescues the cuticle hypopigmentation phenotype caused by expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^pnr-MD237.

Co-expression of Scox^GD898 does not suppress the cuticle hypopigmentation phenotype caused by expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^pnr-MD237.

Co-expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} suppresses the rough eye phenotype caused by expression of Ctr1B^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PFa.

Co-expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} has no effect on the eye phenotype caused by expression of Ctr1A^GD16726 under the control of Scer\GAL4^GMR.PFa.

(Binks et al., 2010)

Expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^pnr-MD237 in a Mvl[97f]/+ background does not significantly affect pigmentation or thoracic development compared to overexpression in a wildtype background.

Simultaneous overexpression of ATP7^{Scer\UAS.T:Zzzz\FLAG} and Mvl^{Scer\UAS.T:Zzzz\FLAG} by Scer\GAL4^pnr-MD237 causes severe hypopigmentation and a further reduction in thorax width compared to overexpression of ATP7^{Scer\UAS.T:Zzzz\FLAG} alone.

(Southon et al., 2008)

The loss of pigmentation seen in a stripe down the midline of the notum of ATP7^{Scer\UAS.T:Zzzz\FLAG}; Scer\GAL4^pnr-MD237 adults is suppressed by Ctr1A^{Scer\UAS.T:Zzzz\FLAG} but not by CCS^{Scer\UAS.T:Zzzz\FLAG} or Atox1^{Scer\UAS.T:Zzzz\FLAG}.

(Norgate et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^pnr-MD237/ATP7^UAS.Tag:FLAG rescues ATP7^GD3322

(Binks et al., 2010)

Scer\GAL4^Act5C.PI/ATP7^UAS.Tag:FLAG rescues ATP7^ΔP17

(Norgate et al., 2006)

Comments

Co-expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} rescues the cuticle hypopigmentation phenotype caused by expression of ATP7^GD3322 under the control of Scer\GAL4^pnr-MD237.

(Binks et al., 2010)

Lethality due to ATP7^ΔP17/Y is rescued by ATP7^{Scer\UAS.T:Zzzz\FLAG}; Scer\GAL4^Act5C.PI.

(Norgate et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ATP7^{Scer\UAS.T:Zzzz\FLAG}

ATP7^UAS.Tag:FLAG

Name Synonyms

Secondary FlyBase IDs

References (10)

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