FlyBase Allele Report: Dmel\Atg1[UAS.Tag:MYC]

General Information

Symbol

Dmel\Atg1^UAS.Tag:MYC

Species

D. melanogaster

Name

FlyBase ID

FBal0221500

Feature type

allele

Associated gene

Dmel\Atg1

Associated Insertion(s)

Carried in Construct

P{UAS-Myc-Atg1}

Also Known As

UAS-Myc-Atg1, UAS-Atg1

Key Links

Transgenic product class

wild_type

(Chang and Neufeld, 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Chang and Neufeld, 2009)

Mutagen

in vitro construct

(Chang and Neufeld, 2009)

Progenitor genotype

Carried in construct

P{UAS-Myc-Atg1}

Cytology

Description

UASt regulatory sequences drive expression of the Atg1 coding region, which is tagged with six copies of Tag:MYC.

(Chang and Neufeld, 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Atg1

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | third instar larval stage, with Scer\GAL4^GMR.PU

(Kim et al., 2013)

Phenotype Manifest In

eye disc, with Scer\GAL4^GMR.PU

(Kim et al., 2013)

Detailed Description

Statement

Reference

Expression of Atg1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PU results in the accumulation of acidic vesicles in most of the eye imaginal disc cells posterior to the morphogenetic furrow. There is also ectopic cell death in this area of the eye imaginal disc.

(Kim et al., 2013)

Expression of Atg1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^hs.PB using a 1 hour heat shock results in a weak induction of autophagy; a few, small autophagosomes are seen, mostly confined to the perinuclear region.

Expression of Atg1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP in fat body cell clones has no appreciable effect on cell size.

Expression of Atg1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF has no discernible effect on eye development.

Expression of Atg1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Cg.PA does not affect larval growth or developmental timing.

(Chang and Neufeld, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible | adult stage phenotype, suppressible by Idit^JF02930, Scer\GAL4^GMR.PF

(Cobb et al., 2023)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has increased cell death | adult stage phenotype, suppressible by Idit^JF02930, Scer\GAL4^GMR.PF

(Cobb et al., 2023)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has visible phenotype, suppressible | partially by Gyf^HM05106, Scer\GAL4^GMR.PU

(Kim et al., 2015)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has visible phenotype, suppressible by Atg1^JF02273, Scer\GAL4^GMR.PU

(Kim et al., 2015)

Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has increased cell death | third instar larval stage phenotype, suppressible by Atg17^HMS01611, Scer\GAL4^GMR.PU

(Kim et al., 2013)

NOT suppressed by

Statement

Reference

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has visible phenotype, non-suppressible by eIF4EHP^GL01035, Scer\GAL4^GMR.PU

(Kim et al., 2015)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has visible phenotype, non-suppressible by pico^GD7437, Scer\GAL4^GMR.PU

(Kim et al., 2015)

Suppressor of

Statement

Reference

Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Tyra et al., 2020)

Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor of increased size | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Tyra et al., 2020)

Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor of increased mortality during development phenotype of Scer\GAL4^GMR.PF, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Tyra et al., 2020)

Scer\GAL4^hs.PB/Atg1^UAS.Tag:MYC is a suppressor | partially of lethal | recessive phenotype of Atg13^unspecified

(Chang and Neufeld, 2009)

Other

Statement

Reference

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible | adult stage phenotype

(Cobb et al., 2023)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has increased cell death | adult stage phenotype

(Cobb et al., 2023)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has fertile phenotype

(Kim et al., 2015)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has increased cell death phenotype

(Kim et al., 2015)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has visible phenotype

(Kim et al., 2015)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has sterile phenotype

(Kim et al., 2015)

Atg13^UAS.cCa, Atg1^UAS.Tag:MYC, Scer\GAL4^Act5C.PP has decreased cell size phenotype

(Chang and Neufeld, 2009)

Atg13^UAS.cCa, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype

(Chang and Neufeld, 2009)

Atg13^UAS.cCa, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has lethal | first instar larval stage phenotype

(Chang and Neufeld, 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Idit^JF02930, Scer\GAL4^GMR.PF

(Cobb et al., 2023)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has autolysosome | increased number | adult stage phenotype, suppressible by Idit^JF02930, Scer\GAL4^GMR.PF

(Cobb et al., 2023)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by Gyf^HM05106, Scer\GAL4^GMR.PU

(Kim et al., 2015)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Atg1^JF02273, Scer\GAL4^GMR.PU

(Kim et al., 2015)

Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has eye disc phenotype, suppressible by Atg17^HMS01611, Scer\GAL4^GMR.PU

(Kim et al., 2013)

NOT suppressed by

Statement

Reference

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by eIF4EHP^GL01035, Scer\GAL4^GMR.PU

(Kim et al., 2015)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by pico^GD7437, Scer\GAL4^GMR.PU

(Kim et al., 2015)

Suppressor of

Statement

Reference

Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Tyra et al., 2020)

Other

Statement

Reference

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype

(Cobb et al., 2023)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has autolysosome | increased number | adult stage phenotype

(Cobb et al., 2023)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has eye phenotype

(Kim et al., 2015)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has eye disc phenotype

(Kim et al., 2015)

Atg13^UAS.cCa, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has ommatidium phenotype

(Chang and Neufeld, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Atg1^{Scer\UAS.T:Hsap\MYC} and Atg13^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PU at 18[o]C results in reduced viability and fertility, as well as eye degeneration. Raising the flies at 18[o]C restores fertility, whilst maintaining the eye degeneration. Retinal cells such as photoreceptors and cone cells exhibit degeneration and there is complete disarray of the ommatidia structure and accumulation of membrane-dense vacuoles. After 4 weeks the retinal structure has completely degenerated except for the corneal lens exocuticle. Prominent cell death is seen in the differentiation ommatidia in eye disc and autophagic flux is increased.

Expression of Gyf^HM05106 dramatically suppresses the eye degeneration and eye disc cell death seen when Atg1^{Scer\UAS.T:Hsap\MYC} and Atg13^{Scer\UAS.T:Avic\GFP-EGFP} are co-expressed under the control of Scer\GAL4^GMR.PU at 18[o]C. However, the photoreceptor morphology is not fully restored. The increased autophagic flux in eye discs is also suppressed.

Expression of Atg1^JF02273 completely suppresses the eye degeneration seen when Atg1^{Scer\UAS.T:Hsap\MYC} and Atg13^{Scer\UAS.T:Avic\GFP-EGFP} are co-expressed under the control of Scer\GAL4^GMR.PU at 18[o]C.

Expression of 4EHP^GL01035 does not suppress the eye degeneration seen when Atg1^{Scer\UAS.T:Hsap\MYC} and Atg13^{Scer\UAS.T:Avic\GFP-EGFP} are co-expressed under the control of Scer\GAL4^GMR.PU at 18[o]C.

Expression of pico^GD7437 does not suppress the eye degeneration seen when Atg1^{Scer\UAS.T:Hsap\MYC} and Atg13^{Scer\UAS.T:Avic\GFP-EGFP} are co-expressed under the control of Scer\GAL4^GMR.PU at 18[o]C.

(Kim et al., 2015)

Co-expression of Atg17^HMS01611 almost completely suppresses the accumulation of acidic vesicles and increased cell death seen posterior to the morphogenetic furrow in the eye discs of larvae expressing Atg1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PU.

(Kim et al., 2013)

Co-expression of Atg13^Scer\UAS.cCa results in a marked increase in the number, size, and distribution of autophagosomes in animals expressing Atg1^{Scer\UAS.T:Hsap\MYC} under the control of low levels of Scer\GAL4^hs.PB (using a 1 hour heat shock).

Autophagy is no longer induced by expression of Atg1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Cg.PA in clones of cells that are also homozygous for Atg13^Δ74.

Co-expression of Atg13^Scer\UAS.cCa and Atg1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP in fat body cell clones results in cells that are markedly reduced in size compared to neighbouring control cells.

Co-expression of Atg13^Scer\UAS.cCa and Atg1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF leads to disruption of ommatidial patterning.

Expression of Atg1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^hs.PB can partially rescue the lethality, but not the autophagy defect, of Atg13^unspecified homozygotes.

(Chang and Neufeld, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Atg1^{Scer\UAS.T:Hsap\MYC}

Atg1^UAS.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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