FB2024_03 , released June 25, 2024
Allele: Dmel\Hus1-like37
Open Close
General Information
Symbol
Dmel\Hus1-like37
Species
D. melanogaster
Name
FlyBase ID
FBal0221553
Feature type
allele
Associated gene
Dmel\Hus1-like
Associated Insertion(s)
Carried in Construct
Also Known As
hus137
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Abdu et al., 2007)
Progenitor genotype
P{GT1}BG00590
(Abdu et al., 2007)
Cytology
Description

Mobilisation of P{GT1}BG00590, which is inserted 2kb downstream of Hus1-like, results in a 3297 base deletion, which removes the entire Hus1-like ORF but does not delete any other transcript.

(Abdu et al., 2007)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3R:4,806,605..4,809,903 [-]
Comment:

3297bp deletion resulting from the imprecise excision of P{GT1}BG00590.

(Abdu et al., 2007)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous Hus1-like37 flies show normal locomotor activity rhythms.

      (Lamaze et al., 2011)

      Mutant larvae show cell cycle arrest after a 500Rad irradiation exposure. They also show a normal reduction in BrdU incorporation after a 4000Rad irradiation exposure.

      (Klovstad et al., 2008)

      Hus1-like37 flies are viable although female sterile.

      Hus1-like37 mutant flies are sensitive to hydroxyurea and methyl methanesulfonate (MMS) but not to X-rays (2500 rad). Exposure to 10 or 20 mM hydroxyurea affects the survival of Hus1-like37 mutants, whereas treatment with 30 mM hydroxyurea eliminates almost all Hus1-like37 and Hus1-like37/Df(3R)110 mutant larvae. Relatively low doses of MMS (0.025%) causes almost 100% death of Hus1-like37 and Hus1-like37/Df(3R)110 mutant larvae. Most of the Hus1-like37mutants die as larvae. When Hus1-like37 homozygous first and early second instar larvae are separated from their heterozygous siblings before MMS treatment, only 19% survive to pupal stage, whereas 75% of their heterozygous siblings form pupae. For both genotypes around20% die as pharate adults.

      Approximately 15.% of Hus1-like37 mutant larvae treated with 0.025% methyl methanesulfonate exhibit aneuploid nuclei, an approximately four-fold increase as compared with wild-type.

      Treatment of Hus1-like37 mutant flies with 4000 rads of irradiation does not result in a decresase of homozygous flies relative to untreated controls. Similar to wild-type, Hus1-like37 mutant discs contain very few mitotic cells after irradiation. Four hours after irradiation, Hus1-like37 mutant discs exhibit wild-type levels of apoptosis, indicating that Hus1-like is not required for post-irradiation induction of apoptosis.

      Approximately 92% of eggs laid by Hus1-like37 mutant mothers exhibit nuclear defects, where the nucleus is found in a variety of conformations including the smooth spherical wild-type shape, oblong shape, or in several separate pieces along the nuclear periphery. Similar defects are generated from Hus1-like37/Df(3R)110 mothers.

      (Abdu et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      Hus1-like37/Hus1-like[+] is a suppressor of dorsal appendage phenotype of spn-BBU

      (Abdu et al., 2007)

      Hus1-like37/Hus1-like[+] is a suppressor of egg phenotype of spn-BBU

      (Abdu et al., 2007)

      Hus1-like37/Hus1-like[+] is a suppressor of karyosome phenotype of spn-BBU

      (Abdu et al., 2007)

      Hus1-like37 is a suppressor of dorsal appendage phenotype of spn-BBU

      (Abdu et al., 2007)

      Hus1-like37 is a suppressor of egg phenotype of spn-BBU

      (Abdu et al., 2007)

      Hus1-like37 is a suppressor of karyosome phenotype of spn-BBU

      (Abdu et al., 2007)

      Hus1-like37/Hus1-like[+] is a suppressor of nucleus phenotype of okrAA

      (Abdu et al., 2007)

      Hus1-like37/Hus1-like[+] is a suppressor of egg phenotype of okrAA

      (Abdu et al., 2007)

      Hus1-like37/Hus1-like[+] is a suppressor of oocyte phenotype of okrAA

      (Abdu et al., 2007)

      Hus1-like37/Hus1-like[+] is a suppressor of dorsal appendage phenotype of okrAA

      (Abdu et al., 2007)

      Hus1-like37 is a suppressor of nucleus phenotype of okrAA

      (Abdu et al., 2007)

      Hus1-like37 is a suppressor of egg phenotype of okrAA

      (Abdu et al., 2007)

      Hus1-like37 is a suppressor of oocyte phenotype of okrAA

      (Abdu et al., 2007)

      Hus1-like37 is a suppressor of dorsal appendage phenotype of okrAA

      (Abdu et al., 2007)
      NOT Suppressor of
      Statement
      Reference

      Hus1-like37 is a non-suppressor of karyosome phenotype of okrAA

      (Abdu et al., 2007)

      Hus1-like37/Hus1-like[+] is a non-suppressor of karyosome phenotype of okrAA

      (Abdu et al., 2007)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      A heterozygous Hus1-like37 background partially suppresses the abnormal egg phenotype for eggs laid by spn-BBU homozygous mutant mothers, with only 45% of eggs exhibiting partially or completely fused appendages or lacking appendages altogether, compared to 51% in okrAA mutants.

      A homozygous Hus1-like37 background completely suppresses the abnormal egg phenotype for eggs laid by spn-BBU homozygous mutant mothers.

      A heterozygous Hus1-like37 background has no effect on the abnormal karyosome phenotype associated with spn-BBU homozygous mutants.

      A homozygous Hus1-like37 background has no effect on the abnormal karyosome phenotype associated with spn-BBU homozygous mutants.

      A heterozygous Hus1-like37 background partially suppresses the abnormal egg phenotype for eggs laid by okrAA homozygous mutant mothers, with only 35% of eggs exhibiting partially or completely fused appendages or lacking appendages altogether, compared to 51% in okrAA mutants.

      A homozygous Hus1-like37 background completely suppresses the abnormal egg phenotype for eggs laid by okrAA homozygous mutant mothers.

      A heterozygous Hus1-like37 background has no effect on the abnormal karyosome phenotype associated with okrAA homozygous mutants.

      A homozygous Hus1-like37 background has no effect on the abnormal karyosome phenotype associated with okrAA homozygous mutants.

      (Abdu et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Hus1-like37 is rescued by Scer\GAL4Act5C.PU/Hus1-likefl.UASp

      (Abdu et al., 2007)
      Comments

      Expression of Hus1-likefl.Scer\UAS under the control of Scer\GAL4Act5C rescues the karyosome defects associated with Hus1-like37 mutants.

      (Abdu et al., 2007)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hus1-like37
      (Lamaze et al., 2011)
      hus137
      (Klovstad et al., 2008, Abdu et al., 2007)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)