FB2024_03 , released June 25, 2024
Allele: Dmel\ykiUAS.cZa
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General Information
Symbol
Dmel\ykiUAS.cZa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Zhang
FlyBase ID
FBal0239893
Feature type
allele
Associated gene
Dmel\yki
Associated Insertion(s)
Carried in Construct
P{UAS-yki.Z}
Also Known As
UAS-Yki
Key Links
Transgenic product class
wild_type
(Zhang et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Zhang et al., 2008)
Mutagen
in vitro construct
(Zhang et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-yki.Z}
Cytology
Description

UAS regulatory sequences drive expression of yki.

(Zhang et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
yki
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of ykiUAS.cZa under the control of Scer\GAL4moody.PU induces a compromised blood-brain barrier, as dye injected into the third instar larval body cavity is able to diffuse into the brain. In agreement, larval subperineurial glial cells show disrupted/discontinuous septate junctions (Dlg immunostaining) and significant increases in nuclear size and ploidy, as compared to controls. There is also a significant increase in larval brain volume , as compared to controls.

      (Li et al., 2017)

      Expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU induces adult eye overgrowth.

      (Hu et al., 2016)

      Expression of ykiScer\UAS.cZa under the control of Scer\GAL4esg-NP5130 (restricted to the adult stages using Scer\GAL80ts.αTub84B) results in an increase in intestinal stem cell division.

      Expression of ykiScer\UAS.cZa in enterocytes (ECs) under the control of Scer\GAL4Myo31DF-NP0001 (restricted to the adult stages using Scer\GAL80ts.αTub84B) results in an increase in intestinal stem cell division.

      Intestinal stem cell clones expressing ykiScer\UAS.cZa contain more cells than control clones.

      (Huang et al., 2014)

      Expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU results in enlarged eyes.

      (Huang et al., 2013)

      An increased number of intestinal stem cells (ISCs) and enteroblasts are seen in the posterior midguts of adult flies expressing ykiScer\UAS.cZa under the control of Scer\GAL4esg-NP5130. The number of mitotic cells is also increased, resulting in pronounced hyperplasia of the intestine with a thicker intestinal epithelium composed of a multi-layer of tightly packed cells.

      Overexpression of ykiScer\UAS.cZa in intestinal stem cell clones under the control of Scer\GAL4tub.PU results in a significant increase in cell numbers and in the formation of large clones.

      Expression of ykiScer\UAS.cZa in adult enterocytes under the control of Scer\GAL4Myo31DF.PU results in an increased number of intestinal stem cells and enteroblasts.

      Expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU results in dramatic eye overgrowth.

      (Jin et al., 2013)

      Expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU results in an enlarged eye.

      (Jin et al., 2012)

      Expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF results in eye overgrowth.

      (Yue et al., 2012)

      Overexpression of ykiScer\UAS.cZa in adult intestine stem cells and enteroblasts using Scer\GAL4esg-NP5130 leads to an increase in the number of precursor cells.

      Overexpression of ykiScer\UAS.cZa in absorptive enterocytes in the adult midgut under the control of Scer\GAL4Myo31DF-NP0001 and Scer\GAL80ts.αTub84B induces a dramatic increase in intestine stem cell proliferation.

      (Ren et al., 2010)

      Expression of ykiScer\UAS.cZa under the control of Scer\GAL4hh-Gal4 results in increased size of the posterior compartment in the wing disc.

      Expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF results in overgrown eyes.

      The eye overgrowth phenotype caused by expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF is suppressed by co-expression of either ykidsRNA.N.Scer\UAS or ykidsRNA.C.Scer\UAS.

      (Zhang et al., 2008)

      Expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF results in enlarged eyes.

      Ectopic BrdU incorporation posterior to the second mitotic wave is seen in the eye discs of animals expressing ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF.

      (Zhang et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      Scer\GAL4GMR.PU, ykiUAS.cZa has visible | adult stage phenotype, enhanceable by AckUAS.Tag:V5, Scer\GAL4GMR.PU

      (Hu et al., 2016)

      Scer\GAL4GMR.PU, ykiUAS.cZa has visible phenotype, enhanceable by par-1L-484/Scer\GAL4GMR.PU

      (Huang et al., 2013)

      Scer\GAL4GMR.PU, ykiUAS.cZa has visible phenotype, enhanceable by par-1L-507/Scer\GAL4GMR.PU

      (Huang et al., 2013)

      Scer\GAL4GMR.PU, ykiUAS.cZa has visible phenotype, enhanceable by par-1F-727/Scer\GAL4GMR.PU

      (Huang et al., 2013)

      Scer\GAL4GMR.PU, ykiUAS.cZa has visible phenotype, enhanceable by par-1UAS.Tag:MYC.cHa/Scer\GAL4GMR.PU

      (Huang et al., 2013)

      Scer\GAL4GMR.PU, ykiUAS.cZa has visible phenotype, enhanceable by par-1KD.UAS.Tag:MYC/Scer\GAL4GMR.PU

      (Huang et al., 2013)

      Scer\GAL4GMR.PU, ykiUAS.cZa has visible phenotype, enhanceable by brmD718A.UAS.Tag:FLAG/Scer\GAL4GMR.PU

      (Jin et al., 2013)

      Scer\GAL4NP5130, ykiUAS.cZa has increased occurrence of cell division | adult stage phenotype, enhanceable by brmUAS.Tag:FLAG/Scer\GAL4NP5130

      (Jin et al., 2013)

      Scer\GAL4GMR.PU, ykiUAS.cZa has visible phenotype, enhanceable by hpoK71R.UAS.Tag:FLAG/Scer\GAL4GMR.PU

      (Jin et al., 2012)

      Scer\GAL4GMR.PU, ykiUAS.cZa has visible phenotype, enhanceable by hpoT195A.UAS.Tag:FLAG/Scer\GAL4GMR.PU

      (Jin et al., 2012)

      Scer\GAL4GMR.PU, ykiUAS.cZa has visible phenotype, enhanceable by hpoM242E.UAS.Tag:FLAG/Scer\GAL4GMR.PU

      (Jin et al., 2012)

      Scer\GAL4GMR.PU, ykiUAS.cZa has visible phenotype, enhanceable by hpoΔ238-246.UAS.Tag:FLAG/Scer\GAL4GMR.PU

      (Jin et al., 2012)

      Scer\GAL4GMR.PF, ykiUAS.cZa has visible | adult stage phenotype, enhanceable by edGD2575/Scer\GAL4GMR.PF

      (Yue et al., 2012)

      Scer\GAL4GMR.PF, ykiUAS.cZa has abnormal size | adult stage phenotype, enhanceable by edGD2575/Scer\GAL4GMR.PF

      (Yue et al., 2012)
      NOT Enhanced by
      Statement
      Reference

      Scer\GAL4GMR.PU, ykiUAS.cZa has visible | adult stage phenotype, non-enhanceable by AckKD.UAS.Tag:V5, Scer\GAL4GMR.PU

      (Hu et al., 2016)
      Suppressed by
      Suppressor of
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Statement
      Reference

      Scer\GAL4GMR.PU, ykiUAS.cZa has eye | adult stage phenotype, non-enhanceable by AckKD.UAS.Tag:V5, Scer\GAL4GMR.PU

      (Hu et al., 2016)
      Suppressed by
      Suppressor of
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The eye overgrowth observed in adult flies expressing ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU is increased further by co-expression of AckScer\UAS.T:SV5\V5 (which on its own also promotes eye growth) but not AckKD.Scer\UAS.T:SV5\V5.

      (Hu et al., 2016)

      Expression of bansponge.Scer\UAS.T:Disc\RFP does not suppress the increase in intestinal stem cell division when ykiScer\UAS.cZa is expressed in enterocytes (ECs) under the control of Scer\GAL4Myo31DF-NP0001 (restricted to the adult stages using Scer\GAL80ts.αTub84B).

      Expression of bansponge.Scer\UAS.T:Disc\RFP suppresses the increase in intestinal stem cell division seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4esg-NP5130 (restricted to the adult stages using Scer\GAL80ts.αTub84B).

      Df(3L)banΔ1 suppresses the increase in intestinal stem cell clone size seen when ykiScer\UAS.cZa is expressed.

      (Huang et al., 2014)

      Expression of par-1L-484 enhances the eye overgrowth phenotype seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PU.

      Expression of par-1L-507 enhances the eye overgrowth phenotype seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PU.

      Expression of par-1F-727 enhances the eye overgrowth phenotype seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PU.

      Expression of par-1Scer\UAS.T:Hsap\MYC.cHa enhances the eye overgrowth phenotype seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PU.

      Expression of par-1KD.Scer\UAS.T:Hsap\MYC does not dramatically enhance the eye overgrowth phenotype seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PU.

      Expression of ykiScer\UAS.cZa suppresses the shrunken P-compartment phenotype seen in wing discs when par-1HMS00405 is expressed under the control of Scer\GAL4hh-Gal4.

      (Huang et al., 2013)

      Expression of brmGD4507 greatly suppresses the intestinal stem cell overproliferation and hyperplasia seen when ykiScer\UAS.cZa is expressed in adult flies under the control of Scer\GAL4esg-NP5130.

      brm2 completely suppresses the significant increase in cell numbers seen in intestinal stem cell clones overexpressing of ykiScer\UAS.cZa under the control of Scer\GAL4tub.PU.

      Expression of ykiScer\UAS.cZa enhances the increased intestinal stem cell proliferation seen when brmD718A.Scer\UAS.T:Zzzz\FLAG is expressed in adult flies under the control of Scer\GAL4esg-NP5130.

      Expression of brmN.Scer\UAS.T:Zzzz\FLAG enhances the increased intestinal stem cell proliferation seen when ykiScer\UAS.cZa is expressed in adult flies under the control of Scer\GAL4esg-NP5130.

      Expression of brmC.Scer\UAS.T:Zzzz\FLAG enhances the increased intestinal stem cell proliferation seen when ykiScer\UAS.cZa is expressed in adult flies under the control of Scer\GAL4esg-NP5130.

      Expression of brmScer\UAS.T:Zzzz\FLAG does not significantly affect the eye overgrowth seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PU.

      Expression of brmD718A.Scer\UAS.T:Zzzz\FLAG enhances the eye overgrowth seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PU.

      (Jin et al., 2013)

      Co-expression of hpoScer\UAS.WT.T:Zzzz\FLAG and ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU results in small, rough eyes.

      Co-expression of hpoT195E.Scer\UAS.T:Zzzz\FLAG and ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU results in small, rough eyes.

      Co-expression of hpoI634D.Scer\UAS.T:Zzzz\FLAG suppresses the overgrowth phenotype caused by expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU, with the resulting flies having rough eyes.

      Co-expression of hpoK71R.Scer\UAS.T:Zzzz\FLAG in flies expressing ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU results in a dramatic increase in eye size.

      Co-expression of either hpoT195A.Scer\UAS.T:Zzzz\FLAG, hpoM242E.Scer\UAS.T:Zzzz\FLAG or hpoΔ238-246.Scer\UAS.T:Zzzz\FLAG enhances the eye overgrowth phenotype caused by expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU.

      (Jin et al., 2012)

      Expression of edGD2575 enhances the eye overgrowth seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PF.

      (Yue et al., 2012)

      Coexpression of sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS with ykiScer\UAS.cZa in adult intestine stem cells and enteroblasts using Scer\GAL4esg-NP5130 suppresses the intestine stem cell proliferation seen upon expression of ykiScer\UAS.cZa alone.

      Co-expression of sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS with ykiScer\UAS.cZa in absorptive enterocytes in the adult midgut under the control of Scer\GAL4Myo31DF-NP0001 and Scer\GAL80ts.αTub84B suppresses the proliferation increase in intestine stem cells seen upon expression of ykiScer\UAS.cZa alone.

      (Ren et al., 2010)

      The eye overgrowth phenotype caused by expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF is enhanced by co-expression of sdScer\UAS.T:Ivir\HA1. The eyes are depigmented in these flies.

      The amount of ectopic BrdU incorporation posterior to the second mitotic wave that is seen in the eye discs of flies expressing ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF is enhanced by co-expression of sdScer\UAS.T:Ivir\HA1.

      The enlarged eye phenotype caused by expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF is suppressed by co-expression of either sddsRNA.N.Scer\UAS or sddsRNA.C.Scer\UAS (or both co-expressed together). The ectopic incorporation of BrdU seen in the eye discs of animals expressing ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF is also suppressed by simultaneous co-expression of both sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS.

      (Zhang et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      ykiScer\UAS.cZa
      ykiUAS.cZa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Zhang
      Secondary FlyBase IDs
        References (14)