FlyBase Allele Report: Dmel\Trpγ[1]

General Information

Symbol

Dmel\Trpγ¹

Species

D. melanogaster

Name

FlyBase ID

FBal0240386

Feature type

allele

Associated gene

Dmel\Trpγ

Associated Insertion(s)

TI{TI}Trpγ¹

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Akitake et al., 2015)

Mutagen

ends-out gene targeting

(Akitake et al., 2015)

Progenitor genotype

Associated Insertion(s)

TI{TI}Trpγ¹

Cytology

Description

Deletion removing 155 nucleotides from Trpγ¹ exon 8. This removes sequence encoding amino acid residues 605-656. (Revision of data reported in FBrf0228530).

(Akitake and Montell, 2016.6.1)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:16,666,930..16,667,084 [-]

Comment:

A deletion generated by homologous recombination. Deletion extent determined from primer sequences.

(Akitake and Montell, 2016.6.1)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of autism spectrum disorder

CEA with Trpγ^G4

(Palacios-Muñoz et al., 2022)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage

(Akitake et al., 2015)

fertile

(Akitake et al., 2015)

uncoordinated | adult stage

(Akitake et al., 2015)

uncoordinated | adult stage (with Trpγ^G4)

(Akitake et al., 2015)

viable

(Akitake et al., 2015)

Phenotype Manifest In

scolopale cell

(Akitake et al., 2015)

Detailed Description

Statement

Reference

Trpγ¹ mutant adults show normal preference for less solid sucrose-agarose food than a harder one (higher agarose conc., same sucrose content) one in a two-way food choice assay.

(Zhang et al., 2016)

Adult mutant males and females show significantly decreased locomotor activity compared to wild type (both average daily activity and peak activity are reduced). The flies also show a small but significant defect in negative geotaxis.

Mutant flies require four times as long as wild-type flies to complete the righting reflect.

Mutant flies show significantly reduced speed and precision of movements during walking compared to controls.

Homozygous and Trpγ^G4/Trpγ¹ flies initiate crossing of gaps at a frequency similar to controls, but their ability to successfully traverse larger gaps is impaired compared to wild type. The defect in homozygous is due to an inability to perform all the normal fine postural adjustments while attempting to cross the gap.

Homozygous and Trpγ^G4/Trpγ¹ tibial extensor muscles show a significant reduction in the action potential frequency in response to tibia deflections compared to controls.

(Akitake et al., 2015)

The gustatory aversion of mutant flies to 6mM camphor in a two way-choice test is not significantly different from that seen in wild-type flies.

(Zhang et al., 2013)

Mutant flies exhibit the wild type avoidance of aristolochic acid in food choice assays.

(Kim et al., 2010)

Mutant adults show normal avoidance of 1% citronellal in a direct airborne repellent test (DART) assay.

(Kwon et al., 2010)

Mutant third instar larvae show a slight, but statistically insignificant decrease in the preference for 17.5[o]C over 14[o]C in a two-way choice test compared to wild-type controls.

(Kwon et al., 2010)

trpγ¹ mutants retain a preference for 18[o]C (i.e. are thermotactic).

(Kwon et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Trpγ¹ has uncoordinated | adult stage phenotype, suppressible by Calx^B

(Akitake et al., 2015)

Suppressor of

Statement

Reference

Trpγ¹ is a suppressor of uncoordinated | adult stage phenotype of Calx^B

(Akitake et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Double mutant Trpγ¹ ; Calx^B flies have a significantly less severe defect in their ability to successfully traverse large gaps of 3.5mm compared to either single mutant.

(Akitake et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Trpγ¹/Trpγ^G4 is rescued by Trpγ^UAS.cAa/Scer\GAL4^Trpγ-G4

(Akitake et al., 2015)

Trpγ¹ is rescued by Trpγ^UAS.cAa/Scer\GAL4^iav.PU

(Akitake et al., 2015)

Trpγ¹ is rescued by Trpγ^UAS.cAa/Scer\GAL4^elav.PU

(Akitake et al., 2015)

Trpγ¹ is rescued by Trpγ^CH322-162M11

(Akitake et al., 2015)

Partially rescued by

Trpγ¹ is partially rescued by Trpγ^UAS.cAa/Scer\GAL4^nompA.cK

(Akitake et al., 2015)

Not rescued by

Trpγ¹ is not rescued by Trpγ^UAS.cAa/Scer\GAL4^nompC.PP

(Akitake et al., 2015)

Comments

Trpγ^CH322-162M11 rescues defects in fine motor coordination which are seen in Trpγ¹ flies.

Trpγ^CH322-162M11 rescues the action potential frequency of tibial extensor muscles in response to tibia deflections to wild type in Trpγ¹ flies.

Expression of Trpγ^Scer\UAS.cAa under the control of Scer\GAL4^iav.PU, but not Scer\GAL4^nompC.PP, in Trpγ¹ flies rescues the action potential frequency of tibial extensor muscles in response to tibia deflections to wild type.

Expression of Trpγ^Scer\UAS.cAa under the control of Scer\GAL4^Trpγ-G4 in Trpγ^G4/Trpγ¹ flies rescues the action potential frequency of tibial extensor muscles in response to tibia deflections to wild type and also rescues their ability to successfully traverse large gaps of 3.5mm to wild-type levels.

Expression of Trpγ^Scer\UAS.cAa under the control of either Scer\GAL4^elav.PU or Scer\GAL4^iav.PU (neuronal drivers) rescues the reduced ability of Trpγ¹ flies to successfully traverse large gaps of 3.5mm compared to controls. The defect is partially rescued if Trpγ^Scer\UAS.cAa expression is driven by Scer\GAL4^nompA.cK.

The vacuolar volume of the scolopale cells of the chordotonal organs is reduced compared to wild-type in homozygous animals. Other morphological features of the mutant scolopidia appear ultrastructurally normal.

(Akitake et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

64311

w¹¹¹⁸; TI{TI}Trpγ¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Trpγ¹

trpγ¹

(Pradhan et al., 2024, Dhakal et al., 2022, Palacios-Muñoz et al., 2022, Tian et al., 2022, Rimal and Lee, 2019, Zhang et al., 2016, Akitake et al., 2015, Zhang et al., 2013, Kim et al., 2010, Kwon et al., 2010, Kwon et al., 2010, Kwon et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (15)

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