Amino acid replacement: W111term.
G11788056A
W111term | HisCl1-PA; W111term | HisCl1-PB; W111term | HisCl1-PC; W111term | HisCl1-PD
W111term
G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation
(exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase base
on reported amino acid change.
HisCl1T2/Df(3R)E79 flies are paralysed quicker than control flies at 41[o]C. The mutant flies also take longer to recover when returned to 20[o]C than control flies.
HisCl1T2/Df(3R)E79 flies show no significant difference in the time that exposure to halothane results in lethality compared to controls. The mutant flies lose their postural control faster than controls on exposure to halothane, but they show a normal time of recovery from anaesthesia after exposure to halothane.
The amplitudes of the on- and off-transients of the electroretinogram are higher in HisCl1T2/Df(3R)E79 flies than in wild-type controls. The 0.5mV thresholds of both on- and off-transients of the mutant flies are lower than those of the wild-type controls, indicating a higher absolute sensitivity of both transients in the mutants. The sensitivity of the on- and off-responses is increased to a similar degree.
HisCl1T2/Df(3R)E79 adults show significantly increased survival compared to controls after treatment with 0.5μM or 2.5μM ivermectin.
The electroretinograms of HisCl1T2/Df(3R)E79 flies have the same general shape as those of control flies, and the mean amplitude of the photoreceptor component is similar to that of controls over the entire range of light intensities. However, the amplitude of the on-transient is greater in the mutant flies than in controls over a range of light intensities, while the amplitude of the off-transient is larger than normal in the mutant flies at log Is (-3) and (-2).
The electroretinograms of HisCl1T2/Df(3R)E79 flies appear comparatively resistant to ivermectin, with the transient components apparent in more recordings than in wild-type controls.