FlyBase Allele Report: Hsap\LRRK2[UAS.cVa]

General Information

Symbol

Hsap\LRRK2^UAS.cVa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Venderova

FlyBase ID

FBal0244979

Feature type

allele

Associated gene

Hsap\LRRK2

Associated Insertion(s)

Carried in Construct

P{UAS-hLRRK2.WT}

Also Known As

UAS-hLRRK2 WT, UAS-hLRRK2

Key Links

Transgenic product class

wild_type

(Venderova et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Venderova et al., 2009)

Mutagen

in vitro construct

(Venderova et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-hLRRK2.WT}

Cytology

Description

UASt regulatory sequences drive expression of wild-type Hsap\LRRK2.

(Venderova et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\LRRK2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA

(Penney et al., 2016, Venderova et al., 2009)

model of Parkinson's disease 8

CEA

(Imai et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Parkinson's disease

is ameliorated by S6k^l-1

(Penney et al., 2016)

is ameliorated by eIF4E1^S058911

(Penney et al., 2016)

is ameliorated by Hsap\PARK7^UAS.cYa

(Venderova et al., 2009)

is ameliorated by Hsap\PINK1^UAS.cYa

(Venderova et al., 2009)

is ameliorated by Hsap\PRKN^UAS.cYa

(Venderova et al., 2009)

is exacerbated by Hsap\PINK1^UAS.cYa

(Venderova et al., 2009)

model of Parkinson's disease 8

is exacerbated by neur^{ΔRING.UAS.EGFP}

(Imai et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Hsap\LRRK2^Scer\UAS.cVa under the control of Scer\GAL4^Mhc.PW, Scer\GAL4^G14 or Scer\GAL4^how-24B does not result in any significant difference in amplitude of miniature excitatory junction currents (mEJCs), but does lead to an increase in amplitude of EJCs, and an increase in quantal content of larval neuromuscular junctions, and these phenotypes are present over a range of external calcium concentrations, as compared to controls.

Expression of Hsap\LRRK2^Scer\UAS.cVa under the control of Scer\GAL4^Mhc.PW does not result in a significant difference in number of synaptic boutons, presynaptic release sites, as compared to controls.

(Penney et al., 2016)

Expression of Hsap\LRRK2^Scer\UAS.cVa under the control of Scer\GAL4^ple.PU (together with tub-Gal80[ts] to limit the time of expression to adulthood) does not significantly change the number of dopaminergic neurons in the PPM1/2 cluster in the adult brain.

(Imai et al., 2015)

50 day old adults expressing Hsap\LRRK2^Scer\UAS.cVa under the control of Scer\GAL4^ple.PF at room temperature show loss of dopaminergic neurons in the dorsomedial posterior protocerebral clusters (PPM1/2) and in the dorsolateral posterior protocerebral cluster (PPL1) of the brain compared to control flies of the same age. A similar loss of neurons is seen in 20 day old adults expressing Hsap\LRRK2^Scer\UAS.cVa under the control of Scer\GAL4^ple.PF at 29[o]C.

Expression of Hsap\LRRK2^Scer\UAS.cVa under the control of either Scer\GAL4^elav.PU or Scer\GAL4^da.G32 at room temperature significantly extends the basal lifespan of flies compared to controls. Expression of Hsap\LRRK2^Scer\UAS.cVa under the control of Scer\GAL4^elav.PU at 29[o]C has no significant effect on basal lifespan.

Flies expressing Hsap\LRRK2^Scer\UAS.cVa under the control of Scer\GAL4^da.G32 have a significantly greater number of progeny compared with control flies.

Flies expressing Hsap\LRRK2^Scer\UAS.cVa under the control of Scer\GAL4^da.G32 at room temperature show a greater sensitivity to chronic exposure to low levels of rotenone then control flies.

Flies expressing Hsap\LRRK2^Scer\UAS.cVa under the control of Scer\GAL4^GMR.PF at 29[o]C show loss of pigmentation in the eyes. Significantly more black lesions are seen in the mutant eyes than in controls. Ultrastructurally, severe disruption of the regular trapezoidal arrangement of the photoreceptor cells is seen. The cell lattice between photoreceptor cell arrays of different ommatidia is completely absent and the ommatidia are sometimes fused together. Large holes are often seen in the eye sections.

(Venderova et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hsap\LRRK2^UAS.cVa, Scer\GAL4^Mhc.PW has abnormal neurophysiology | third instar larval stage phenotype, suppressible | partially by eIF4E1^S058911/eIF4E1[+]

(Penney et al., 2016)

Hsap\LRRK2^UAS.cVa, Scer\GAL4^Mhc.PW has abnormal neurophysiology | third instar larval stage phenotype, suppressible by S6k[+]/S6k^l-1

(Penney et al., 2016)

Enhancer of

Statement

Reference

Hsap\LRRK2^UAS.cVa/Scer\GAL4^nSyb.PP is an enhancer of decreased cell number | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Hsap\LRRK2^UAS.cVa/Scer\GAL4^nSyb.PP is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Hsap\LRRK2^UAS.cVa/Scer\GAL4^dpp.PU is an enhancer of visible | adult stage phenotype of neur^{ΔRING.UAS.EGFP}

(Imai et al., 2015)

Hsap\LRRK2^UAS.cVa, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, foxo^UAS.cKb

(Kanao et al., 2010)

NOT Enhancer of

Statement

Reference

Hsap\LRRK2^UAS.cVa, Scer\GAL4^dpp.PU is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^dpp.PU, Ser^UAS.cUa

(Imai et al., 2015)

Hsap\LRRK2^UAS.cVa/Scer\GAL4^dpp.PU is a non-enhancer of visible | adult stage phenotype of N^55e11

(Imai et al., 2015)

NOT Suppressor of

Statement

Reference

Hsap\LRRK2^UAS.cVa/Scer\GAL4^how-24B is a non-suppressor of abnormal neurophysiology | third instar larval stage phenotype of GluRIIA^SP16/Df(2L)cl-h4

(Penney et al., 2016)

Hsap\LRRK2^UAS.cVa, Scer\GAL4^Mhc.PW is a non-suppressor of abnormal neurophysiology | third instar larval stage phenotype of Lrrk^UAS.cIa, Scer\GAL4^Mhc.PW

(Penney et al., 2016)

Hsap\LRRK2^UAS.cVa, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Hsap\PRKN^UAS.cYa, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Other

Statement

Reference

Delta^UAS.cUa, Hsap\LRRK2^UAS.cVa, Scer\GAL4^dpp.PU has visible | adult stage phenotype

(Imai et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsap\LRRK2^UAS.cVa, Scer\GAL4^Mhc.PW has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible | partially by eIF4E1^S058911/eIF4E1[+]

(Penney et al., 2016)

Hsap\LRRK2^UAS.cVa, Scer\GAL4^Mhc.PW has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by S6k[+]/S6k^l-1

(Penney et al., 2016)

Hsap\LRRK2^UAS.cVa, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by Hsap\PINK1^UAS.cYa, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

NOT suppressed by

Statement

Reference

Hsap\LRRK2^UAS.cVa, Scer\GAL4^GMR.PF has pigment cell phenotype, non-suppressible by Hsap\PINK1^UAS.cYa, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Enhancer of

Statement

Reference

Hsap\LRRK2^UAS.cVa/Scer\GAL4^nSyb.PP is an enhancer of outer medulla | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Hsap\LRRK2^UAS.cVa/Scer\GAL4^nSyb.PP is an enhancer of medullary intrinsic neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Hsap\LRRK2^UAS.cVa/Scer\GAL4^nSyb.PP is an enhancer of dopaminergic medulla neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Hsap\LRRK2^UAS.cVa/Scer\GAL4^dpp.PU is an enhancer of wing margin phenotype of neur^{ΔRING.UAS.EGFP}

(Imai et al., 2015)

Hsap\LRRK2^UAS.cVa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, foxo^UAS.cKb

(Kanao et al., 2010)

Hsap\LRRK2^UAS.cVa, Scer\GAL4^GMR.PF is an enhancer of interommatidial bristle phenotype of Hsap\PINK1^UAS.cYa, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

NOT Enhancer of

Statement

Reference

Hsap\LRRK2^UAS.cVa, Scer\GAL4^dpp.PU is a non-enhancer of wing margin bristle | increased number phenotype of Scer\GAL4^dpp.PU, Ser^UAS.cUa

(Imai et al., 2015)

Hsap\LRRK2^UAS.cVa/Scer\GAL4^dpp.PU is a non-enhancer of wing vein | adult stage phenotype of N^55e11

(Imai et al., 2015)

NOT Suppressor of

Statement

Reference

Hsap\LRRK2^UAS.cVa/Scer\GAL4^how-24B is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of GluRIIA^SP16/Df(2L)cl-h4

(Penney et al., 2016)

Hsap\LRRK2^UAS.cVa, Scer\GAL4^Mhc.PW is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Lrrk^UAS.cIa, Scer\GAL4^Mhc.PW

(Penney et al., 2016)

Hsap\LRRK2^UAS.cVa, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\PRKN^UAS.cYa, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Other

Statement

Reference

Delta^UAS.cUa, Hsap\LRRK2^UAS.cVa, Scer\GAL4^dpp.PU has wing margin phenotype

(Imai et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

S6k^l-1/+ fully suppresses, and eIF4E1^S058911/+ partially suppresses the increase in excitatory junction current amplitude and quantal content seen in larvae NMJs expressing Hsap\LRRK2^Scer\UAS.cVa under the control of Scer\GAL4^Mhc.PW.

Expression of Hsap\LRRK2^Scer\UAS.cVa under the control of Scer\GAL4^how-24B fails to suppress the increased quantal content observed in GluRIIA^SP16/Df(2L)cl-h4 mutant larval neuromuscular junctions.

Co-expression of Hsap\LRRK2^Scer\UAS.cVa fails to suppress the increase in excitatory junction current amplitude and quantal content seen in larvae NMJs expressing Lrrk^Scer\UAS.cIa under the control of Scer\GAL4^Mhc.PW.

(Penney et al., 2016)

The ectopic wing margin bristle phenotype characteristic for adult flies expressing Ser^Scer\UAS.cUa under the control of Scer\GAL4^dpp.PU is not significantly modified by co-expression of Hsap\LRRK2^Scer\UAS.cVa.

Flies expressing Dl^Scer\UAS.cUa under the control of Scer\GAL4^dpp.PU and kept at 18[o]C do not display any overt wing phenotype but show notching phenotype upon co-expression of Hsap\LRRK2^Scer\UAS.cVa.

The vein thickening and ectopic veins phenotype characteristic for N^55e11 mutant adults is not modified by expression of Hsap\LRRK2^Scer\UAS.cVa under the control of Scer\GAL4^dpp.PU in the mutant background.

The wing notching phenotype characteristic for adults expressing neur^{ΔRING.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^dpp.PU is exacerbated by co-expression of Hsap\LRRK2^Scer\UAS.cVa.

(Imai et al., 2015)

The severity of the eye defects caused by expression of foxo^Scer\UAS.cKb under the control of Scer\GAL4^GMR.PF are enhanced by co-expression of Hsap\LRRK2^Scer\UAS.cVa.

(Kanao et al., 2010)

Co-expression of Hsap\PINK1^Scer\UAS.cYa does not significantly suppress the loss of pigmentation in the eye, the roughness of the eye surface or the formation of holes in eye sections that are seen in flies expressing Hsap\LRRK2^Scer\UAS.cVa under the control of Scer\GAL4^GMR.PF at 29[o]C, but the formation of black lesions in the eye is partially suppressed. The loss of interommatidial bristles that is seen in flies expressing Hsap\PINK1^Scer\UAS.cYa under the control of Scer\GAL4^GMR.PF at 29[o]C is enhanced by co-expression of Hsap\LRRK2^Scer\UAS.cVa.

(Venderova et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\LRRK2^Scer\UAS.cVa

Hsap\LRRK2^UAS.cVa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Venderova

Secondary FlyBase IDs

References (6)

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