FlyBase Allele Report: Dmel\p130CAS[1]

General Information

Symbol

Dmel\p130CAS¹

Species

D. melanogaster

Name

FlyBase ID

FBal0248552

Feature type

allele

Associated gene

Dmel\p130CAS

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

FLPase

Nature of the Allele

Allele class

Mutagen

FLPase

(Tikhmyanova et al., 2010)

Progenitor genotype

Cytology

Description

The complete p130CAS ORF is removed through excision of P{RS5}p130CAS^5-HA-2428 and PBac{WH}f00059

(Tikhmyanova et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:160,802..174,730 [+]

Comment:

Deletion of p130CAS resulting from FLP-catalyzed recombination using the FRT-containing transposons P{RS5}p130CAS^5-HA-2428 and PBac{WH}f00059.

(Tikhmyanova et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Tikhmyanova et al., 2010)

Phenotype Manifest In

embryo | dorsal closure stage

(Tikhmyanova et al., 2010)

Detailed Description

Statement

Reference

Approximately 10% of p130CAS¹ do not hatch but instead develop a "kink" at stage 13, and arrest at stage 15-16 of embryonic development. These embryos exhibit germ band retraction and dorsal closure defects, including an irregular leading edge of migrating cells and typically have an embryonal curvature and a posterior opening in the dorsal cuticle. Similar germ band retraction and dorsal closure defects are seen in 6% of p130CAS¹/Df(3L)Exel6083 and 1% of p130CAS¹/p130CAS^NP4466 embryos.

No lethality is seen in p130CAS¹ heterozygotes.

Germ band retraction and dorsal closure defects are seen in 1% of p130CAS¹/p130CAS^NP4466 embryos.

(Tikhmyanova et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Fak^CG1, p130CAS¹ has lethal | embryonic stage phenotype

(Tikhmyanova et al., 2010)

Src42A^k10108/Src42A[+], p130CAS¹ has partially lethal - majority live | embryonic stage phenotype

(Tikhmyanova et al., 2010)

mew[+]/mew^G0429, p130CAS¹ has partially lethal - majority live phenotype

(Tikhmyanova et al., 2010)

if^B2, p130CAS¹ has partially lethal - majority die | embryonic stage phenotype

(Tikhmyanova et al., 2010)

mys¹/mys[+], p130CAS¹ has partially lethal - majority die | embryonic stage phenotype

(Tikhmyanova et al., 2010)

Src42A^Jp45/Src42A[+], p130CAS¹ has partially lethal - majority die | embryonic stage phenotype

(Tikhmyanova et al., 2010)

mew^EY09631, p130CAS¹ has partially lethal - majority live phenotype

(Tikhmyanova et al., 2010)

Phenotype Manifest In

Other

Statement

Reference

Fak^CG1, p130CAS¹ has embryo | late embryonic stage phenotype

(Tikhmyanova et al., 2010)

Src42A^myri, p130CAS¹ has embryo | late embryonic stage phenotype

(Tikhmyanova et al., 2010)

Src42A^myri, p130CAS¹ has larva | first instar larval stage phenotype

(Tikhmyanova et al., 2010)

Src42A^k10108/Src42A[+], p130CAS¹ has wing phenotype

(Tikhmyanova et al., 2010)

Src42A^E1/Src42A[+], p130CAS¹ has wing phenotype

(Tikhmyanova et al., 2010)

if³, p130CAS¹ has wing phenotype

(Tikhmyanova et al., 2010)

mew[+]/mew^G0429, p130CAS¹ has wing phenotype

(Tikhmyanova et al., 2010)

Fak^CG1, p130CAS¹ has larva | first instar larval stage phenotype

(Tikhmyanova et al., 2010)

Src42A^k10108, p130CAS¹ has embryonic head phenotype

(Tikhmyanova et al., 2010)

Src42A^k10108, p130CAS¹ has embryo | late embryonic stage phenotype

(Tikhmyanova et al., 2010)

Src42A^k10108, p130CAS¹ has larva | first instar larval stage phenotype

(Tikhmyanova et al., 2010)

Src42A^E1, p130CAS¹ has embryonic head phenotype

(Tikhmyanova et al., 2010)

Src42A^E1, p130CAS¹ has embryo | late embryonic stage phenotype

(Tikhmyanova et al., 2010)

Src42A^E1, p130CAS¹ has larva | first instar larval stage phenotype

(Tikhmyanova et al., 2010)

Src42A^myri, p130CAS¹ has embryonic head phenotype

(Tikhmyanova et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Fak56D^CG1/+; p130CAS¹/Df(3L)Exel6083 adults do not emerge, indicating complete lethality. Analysis of the Fak56D^CG1/Fak56D^CG1; p130CAS¹/+ lethal phenotype indicates that most (95%) of the embryos do not hatch. The few escapers survive to pupal stages, but do not emerge. Only 5% of Fak56D^CG1; p130CAS¹ double homozygotes produce cuticles and almost all are marked by dorsal and/or ventral holes, indicating dorsal closure defects. These cuticles also show fused or missing denticle belts.

Src42A^k10108/+; p130CAS¹/Df(3L)Exel6083 mutants are semi-viable, emerging at approximately 50% of the rate of phenotypically normal adult siblings from the same cross.

The low percentage of Src42A^k10108/+; p130CAS¹/p130CAS¹ adult escapers manifest wing blister defects.

Approximately 90% of Src42A^k10108; p130CAS¹ double mutants have holes in or completely absent head cuticles and 10% of these embryos exhibit germ band retraction cuticle defects.

The low percentage of Src42A^E1/+; p130CAS¹/p130CAS¹ adult escapers manifest wing blister defects.

Approximately 90% of Src42A^E1; p130CAS¹ double mutants have holes in or completely absent head cuticles and 10% of these embryos exhibit germ band retraction cuticle defects.

Approximately 90% of Src42A^myri; p130CAS¹ double mutants have holes in or completely absent head cuticles and 10% of these embryos exhibit germ band retraction cuticle defects.

Approximately 27% of if³; p130CAS¹ double homozygous mutants and 25% of if³/if³; p130CAS¹/+ mutants emerge as adults with significantly exacerbated wing blistering defects.

Approximately 56% of if^B2/+; p130CAS¹/p130CAS¹ double mutants emerge as adults.

Approximately 24% of mys¹/+; p130CAS¹/p130CAS¹ double mutants emerge as adults.

Approximately 29% of Src42A^Jp45/+; p130CAS¹/p130CAS¹ double mutants emerge as adults.

p130CAS¹ heterozygotes and homozygotes exhibit a dramatic reduction in the viability of adult mew^EY09631 homozygotes. Moreover, 2% of mew^EY09631/+; p130CAS¹/p130CAS¹ flies have wing blisters and smaller, more rounded wings.

mew^G0429/+; p130CAS¹/p130CAS¹ flies exhibit 29% viability, with escaper flies exhibit wing blisters and smaller, more rounded wings.

(Tikhmyanova et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

p130CAS¹ is rescued by p130CAS^UAS.GFP/Scer\GAL4^hs.PB

(Tikhmyanova et al., 2010)

p130CAS^NP4466/p130CAS¹ is rescued by p130CAS^UAS.GFP/Scer\GAL4^hs.PB

(Tikhmyanova et al., 2010)

Comments

Expression of p130CAS^{Scer\UAS.T:Avic\GFP} (under the control of Scer\GAL4^hs.PB) completely rescues the embryonic germ band retraction and dorsal closure defects observed in p130CAS¹ homozygotes.

Expression of p130CAS^{Scer\UAS.T:Avic\GFP} (under the control of Scer\GAL4^hs.PB) completely rescues the embryonic germ band retraction and dorsal closure defects observed in p130CAS¹/p130CAS^NP4466 trans-heterozygotes.

(Tikhmyanova et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Dcas¹

(Tikhmyanova et al., 2010)

p130CAS¹

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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