FlyBase Allele Report: Dmel\pyd[ex147]

General Information

Symbol

Dmel\pyd^ex147

Species

D. melanogaster

Name

FlyBase ID

FBal0248975

Feature type

allele

Associated gene

Dmel\pyd

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Djiane et al., 2011)

Progenitor genotype

P{GawB}pyd^NP4400

(Djiane et al., 2011)

Cytology

Description

Imprecise excision of the progenitor insertion, P{GawB}pyd^NP4400, results in a deletion encompassing a large region of pyd.

(Djiane et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Carrasco-Rando et al., 2019, Djiane et al., 2011)

majority die during embryonic stage (with pyd^B12)

(Choi et al., 2011)

some die during embryonic stage

(Choi et al., 2011)

some die during embryonic stage (with pyd^B12)

(Choi et al., 2011)

viable

(Carrasco-Rando et al., 2019, Djiane et al., 2011)

visible (with Df(3R)p-XT103)

(Djiane et al., 2011)

visible (with pyd^tam)

(Djiane et al., 2011)

Phenotype Manifest In

basement membrane | embryonic stage 16

(Carrasco-Rando et al., 2019)

dorsocentral bristle | increased number (with Df(3R)p-XT103)

(Djiane et al., 2011)

embryo | dorsal closure stage

(Choi et al., 2011)

embryo | dorsal closure stage (with pyd^B12)

(Choi et al., 2011)

embryonic garland cell | embryonic stage 16

(Carrasco-Rando et al., 2019)

embryonic head

(Choi et al., 2011)

embryonic head (with pyd^B12)

(Choi et al., 2011)

embryonic leading edge cell

(Choi et al., 2011)

embryonic leading edge cell (with pyd^B12)

(Choi et al., 2011)

female germline stem cell | increased number (with Df(3R)p-XT103)

(Djiane et al., 2011)

germarium cap cell | increased number

(Djiane et al., 2011)

germarium cap cell | increased number (with Df(3R)p-XT103)

(Djiane et al., 2011)

macrochaeta | increased number

(Carrasco-Rando et al., 2019)

septate junction | ectopic | embryonic stage 16

(Carrasco-Rando et al., 2019)

slit diaphragm | embryonic stage 16

(Carrasco-Rando et al., 2019)

thorax

(Carrasco-Rando et al., 2019)

ventral head epidermis

(Choi et al., 2011)

ventral head epidermis (with pyd^B12)

(Choi et al., 2011)

wing (with Df(3R)p-XT103)

(Djiane et al., 2011)

wing (with pyd^tam)

(Djiane et al., 2011)

Detailed Description

Statement

Reference

The nephrocytes of pyd^ex147 homozygous stage 16 embryos are abnormally agglutinated, with electron-dense regions at membranes contact sites, thickening of the basement membrane, and complete absence of slit diaphragms; as in the wild-type controls, the only junctions between adjacent nephrocytes are found at the most external region contacting the basement membrane; however, the septate junction markers Dlg, Fas3 and Lgl localize at ectopic junctional complexes.

Nearly all pyd^ex147 adults exhibit extra dorsal thoracic macrochaeta.

(Carrasco-Rando et al., 2019)

Embryos maternally and zygotically mutant for pyd^ex180 exhibit 86% embryonic lethality.

Substantial embryonic lethality (43-58%) is found in flies from pyd^ex147 mothers crossed with pyd^B12 fathers.

The ventral epidermis is intact in pyd^ex147 maternal/zygotic mutant embryos and the CNS is not obviously hypertrophied. While approximately 26% of pyd^ex147 maternal/zygotic mutant embryos appear wild-type, most mutant embryos (45%) exhibit defects in head involution that result in defective head skeletons, whereas the most severe (29%) display complete failure of head involution, leading to a hole in the cuticle. In addition, some show minor defects in dorsal closure.

The ventral epidermis is intact in pyd^B12/pyd^ex147 maternal/zygotic mutant embryos and the CNS is not obviously hypertrophied. While approximately 17% of pyd^B12/pyd^ex147 maternal/zygotic mutant embryos appear wild-type, most mutant embryos (64%) exhibit defects in head involution that result in defective head skeletons, whereas the most severe (19%) display complete failure of head involution, leading to a hole in the cuticle. In addition, some show minor defects in dorsal closure.

(Choi et al., 2011)

Over 90% of pyd^ex147/Df(3R)p-XT103 flies display more than four dorsocentral macrochaetae.

Wings from pyd^ex147/Df(3R)p-XT103 flies are broader compared to control wings. A 10% increase in the number of cells found between the L3 and L4 veins is observed. In third instar larval wing discs, mutant cells appear smaller, and there are 15% more cells per surface unit compared to controls.

Wings from pyd^ex147/pyd^tam flies are broader compared to control wings.

Both pyd^ex147/+ and pyd^ex147/Df(3R)p-XT103 mutants display an increase in the number of ovarian niche cap cells.

An increase in the number of stem cells per ovariole is observed in pyd^ex147/Df(3R)p-XT103 flies.

(Djiane et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

pyd^tam/pyd^ex147 has visible phenotype, non-enhanceable by N[+]/N^55e11

(Djiane et al., 2011)

NOT Enhancer of

Statement

Reference

pyd^ex147/pyd[+] is a non-enhancer of visible | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

NOT Suppressor of

Statement

Reference

pyd^ex147/pyd[+] is a non-suppressor of visible | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

pyd^ex147/Df(3R)p-XT103 has dorsocentral bristle | increased number phenotype, enhanceable by N[+]/N^55e11

(Djiane et al., 2011)

pyd^tam/pyd^ex147 has wing phenotype, enhanceable by ex[+]/ex^e1

(Djiane et al., 2011)

NOT Enhanced by

Statement

Reference

pyd^tam/pyd^ex147 has wing phenotype, non-enhanceable by N[+]/N^55e11

(Djiane et al., 2011)

pyd^tam/pyd^ex147 has wing phenotype, non-enhanceable by Pten^dj189/Pten[+]

(Djiane et al., 2011)

pyd^tam/pyd^ex147 has wing phenotype, non-enhanceable by Pten^c494/Pten[+]

(Djiane et al., 2011)

pyd^tam/pyd^ex147 has wing phenotype, non-enhanceable by Su(dx)^sp/Df(2L)Exel6006

(Djiane et al., 2011)

pyd^tam/pyd^ex147 has wing phenotype, non-enhanceable by Su(H)⁸/Su(H)[+]

(Djiane et al., 2011)

pyd^tam/pyd^ex147 has wing phenotype, non-enhanceable by Su(dx)^sp/Su(dx)[+]

(Djiane et al., 2011)

pyd^tam/pyd^ex147 has wing phenotype, non-enhanceable by yps[+]/yps^JM2

(Djiane et al., 2011)

pyd^tam/pyd^ex147 has wing phenotype, non-enhanceable by Cdk4³/Cdk4[+]

(Djiane et al., 2011)

pyd^tam/pyd^ex147 has wing phenotype, non-enhanceable by Cdk4^k06503/Cdk4[+]

(Djiane et al., 2011)

pyd^tam/pyd^ex147 has wing phenotype, non-enhanceable by Tor[+]/mTor^ΔP

(Djiane et al., 2011)

Suppressed by

Statement

Reference

pyd^ex147 has germarium cap cell | increased number phenotype, suppressible by N[+]/N^55e11

(Djiane et al., 2011)

pyd^ex147 has germarium cap cell | increased number phenotype, suppressible by Su(dx)⁵⁶/Su(dx)³²

(Djiane et al., 2011)

Enhancer of

Statement

Reference

pyd^ex147/pyd[+] is an enhancer of dorsocentral bristle | increased number phenotype of Scer\GAL4^dpp.PU, Su(dx)^UAS.cCa

(Djiane et al., 2011)

NOT Enhancer of

Statement

Reference

pyd^ex147/pyd[+] is a non-enhancer of eye | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

NOT Suppressor of

Statement

Reference

pyd^ex147/pyd[+] is a non-suppressor of eye | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype observed in adults expressing Magi^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PU is not modified by combination with single copy of pyd^ex147.

(Zaessinger et al., 2015)

100% of N^55e11/+; pyd^ex147/Df(3R)p-XT103 flies display more than four dorsocentral macrochaetae.

The broader wings observed in pyd^ex147/pyd^tam flies are not enhanced in a N^55e11/+ background.

N^55e11/+ suppresses the increased ovarian cap cell numbers observed in pyd^ex147/+ mutants.

The increase in the number of ovarian cap cells observed in pyd^ex147/+ animals is suppressed in a Su(dx)³²/Su(dx)⁵⁶ background.

The increase in the number of ovarian cap cells observed in pyd^ex147/pyd^ex147 animals is not suppressed in a Su(dx)³²/Su(dx)⁵⁶ background.

The increased number of dorsocentral macrochaetae in animals expressing Su(dx)^Scer\UAS.cCa under the control of Scer\GAL4^dpp.PU is enhanced in a pyd^ex147/+ background.

Wings from pyd^ex147/pyd^tam flies are broader compared to control wings, and this phenotype is enhanced in a ex^e1/+ background.

Wings from pyd^ex147/pyd^tam flies are broader compared to control wings, and this phenotype is not modified in a Su(H)⁸/+, Su(dx)^sp/+, yps^JM2/+, Cdk4³/+, Cdk4^k06503/+, Tor^ΔP/+, Pten^dj189/+, or Pten^c494/+ background.

Wings from pyd^ex147/pyd^tam flies are broader compared to control wings, and this phenotype is not modified in a Su(dx)^sp/Df(2L)Exel6006 background.

(Djiane et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

pyd^tam/pyd^ex147 is rescued by Scer\GAL4^Act5C.PU/pyd^UAS.GFP

(Djiane et al., 2011)

pyd^tam/pyd^ex147 is rescued by pyd^{ΔEx6.UAS.GFP}/Scer\GAL4^Act5C.PU

(Djiane et al., 2011)

pyd^tam/pyd^ex147 is rescued by Scer\GAL4^Act5C.PU/pyd^{ΔPDZ1.UAS.GFP}

(Djiane et al., 2011)

pyd^tam/pyd^ex147 is rescued by Scer\GAL4^Act5C.PU/pyd^{ΔSH3.UAS.GFP}

(Djiane et al., 2011)

Partially rescued by

pyd^ex147 is partially rescued by Scer\GAL4^Act.PU/pyd^UAS.GFP

(Carrasco-Rando et al., 2019)

pyd^ex147 is partially rescued by pyd^UAS.P/Scer\GAL4^pros.PU

(Carrasco-Rando et al., 2019)

pyd^ex147 is partially rescued by pyd^ΔCt.UAS.P/Scer\GAL4^pros.PU

(Carrasco-Rando et al., 2019)

pyd^tam/pyd^ex147 is partially rescued by Scer\GAL4^Act5C.PU/pyd^{ΔPDZ3.UAS.GFP}

(Djiane et al., 2011)

Not rescued by

pyd^ex147 is not rescued by pyd^ΔCC.UAS.P/Scer\GAL4^pros.PU

(Carrasco-Rando et al., 2019)

pyd^tam/pyd^ex147 is not rescued by Scer\GAL4^Act5C.PU/pyd^{ΔPro-Rich.UAS.GFP}

(Djiane et al., 2011)

pyd^tam/pyd^ex147 is not rescued by Scer\GAL4^Act5C.PU/pyd^{ΔPDZ2.UAS.GFP}

(Djiane et al., 2011)

pyd^tam/pyd^ex147 is not rescued by Scer\GAL4^Act5C.PU/pyd^{ΔGUK.UAS.GFP}

(Djiane et al., 2011)

Comments

The increased size of adult wings in pyd^ex147/pyd^tam mutant flies is restored in animals expressing pyd^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Act5C.PU.

The increased size of adult wings in pyd^ex147/pyd^tam mutant flies is restored in animals expressing pyd^{ΔPDZ1.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Act5C.PU.

The increased size of adult wings in pyd^ex147/pyd^tam mutant flies is restored in animals expressing pyd^{ΔEx6.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Act5C.PU.

The increased size of adult wings in pyd^ex147/pyd^tam mutant flies is not restored in animals expressing pyd^{ΔPDZ2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Act5C.PU.

The increased size of adult wings in pyd^ex147/pyd^tam mutant flies is not restored in animals expressing pyd^{ΔSH3.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Act5C.PU.

The increased size of adult wings in pyd^ex147/pyd^tam mutant flies is not restored in animals expressing pyd^{ΔGUK.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Act5C.PU.

The increased size of adult wings in pyd^ex147/pyd^tam mutant flies is not restored in animals expressing pyd^{ΔPro-Rich.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Act5C.PU.

The increased size of adult wings in pyd^ex147/pyd^tam mutant flies is partially restored in animals expressing pyd^{ΔPDZ3.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Act5C.PU.

(Djiane et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

pyd¹⁴⁷

(Shimizu et al., 2017)

pyd^EX147

(Carrasco-Rando et al., 2023)

pyd^ex147

(Malin et al., 2022, Carrasco-Rando et al., 2019, Zaessinger et al., 2015, Choi et al., 2011, Djiane et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (7)

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