A 2684 base pair fragment from the flanking non-coding or intronic region of nAcRalpha-7E is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.
Optogenetic activation (using Crei\ChR2H134R.UAS.mCherry with blue light) of Scer\GAL4GMR20D01 cells (in a blind norpA7 background) in adults results in an inhibitory response in vertical system cells, relative to baseline.
Inhibition (using Ctet\tetXTNT.UAS) of Scer\GAL4GMR20D01 cells in adults does not affect depolarization of vertical system cells in response to downwards moving visual stimuli, but blocks hyperpolarization in response to upwards moving visual stimuli, compared to controls.