FlyBase Allele Report: Hsap\UCP2[UAS.cFa]

General Information

Symbol

Hsap\UCP2^UAS.cFa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Fridell

FlyBase ID

FBal0256679

Feature type

allele

Associated gene

Hsap\UCP2

Associated Insertion(s)

Carried in Construct

P{UAS-UCP2.F}

Also Known As

UAS-hUCP2

Key Links

Transgenic product class

cDNA

(Fridell et al., 2005)

wild_type

(Fridell et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Fridell et al., 2005)

wild_type

(Fridell et al., 2005)

Mutagen

in vitro construct

(Fridell et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-UCP2.F}

Cytology

Description

UASt drives expression of a full-length Hsap\UCP2 cDNA.

(Fridell et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\UCP2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Besson et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^ple.PF

(Islam et al., 2012)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PF

(Hwang et al., 2014)

abnormal oxidative stress response | adult stage, with Scer\GAL4^Ilp2.PR

(Fridell et al., 2009)

abnormal starvation stress response | adult stage, with Scer\GAL4^Ilp2.PR

(Fridell et al., 2009)

abnormal stress response | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Fridell et al., 2005)

chemical resistant, with Scer\GAL4^ple.PF

(Islam et al., 2012)

chemical resistant | embryonic stage, with Scer\GAL4^ple.PF

(Hwang et al., 2014)

lethal, with Scer\GAL4^Act.PU

(Fridell et al., 2005)

lethal, with Scer\GAL4^Tub.PU

(Fridell et al., 2005)

long lived, with Scer\GAL4^Ilp2.PR

(Fridell et al., 2009)

long lived, with Scer\GAL4^ple.PF

(Islam et al., 2012)

long lived | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Fridell et al., 2005)

Phenotype Manifest In

dopaminergic neuron | adult stage, with Scer\GAL4^ple.PF

(Hwang et al., 2014)

dopaminergic neuron | embryonic stage, with Scer\GAL4^ple.PF

(Hwang et al., 2014)

mitochondrion | adult stage, with Scer\GAL4^ple.PF

(Hwang et al., 2014)

mitochondrion | embryonic stage, with Scer\GAL4^ple.PF

(Hwang et al., 2014)

mitochondrion | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Fridell et al., 2005)

Detailed Description

Statement

Reference

Embryonic dopaminergic neurons in primary culture expressing Hsap\UCP2^Scer\UAS.cFa under the control of Scer\GAL4^ple.PF display resistance to rotenone-treatment induced lethality and mitochondrial fragmentation, and increased mitochondrial motility, as compared to control neurons.

Dopaminergic neurons in flies expressing Hsap\UCP2^Scer\UAS.cFa under the control of Scer\GAL4^ple.PF exhibit mitochondrial aggregation and fusion, as compared to controls.

Flies expressing Hsap\UCP2^Scer\UAS.cFa under the control of Scer\GAL4^GMR.PU do not exhibit any discernible eye phenotype, as compared to controls.

(Hwang et al., 2014)

Flies expressing Hsap\UCP2^Scer\UAS.cFa under the control of Scer\GAL4^ple.PF and raised on rotenone show reduced loss of PAL, PPM1/2, PPM3 and PPL1 dopaminergic neurons compared to wild-type flies raised under the same conditions. The mutant flies also show better survival compared to controls when raised on rotenone.

The progressive loss of climbing ability seen when flies are raised on rotenone is ameliorated if the flies are expressing Hsap\UCP2^Scer\UAS.cFa under the control of Scer\GAL4^ple.PF.

Flies expressing Hsap\UCP2^Scer\UAS.cFa under the control of Scer\GAL4^ple.PF and raised under normal culture conditions have a modest but significant increase in median lifespan compared to controls.

Flies expressing Hsap\UCP2^Scer\UAS.cFa under the control of Scer\GAL4^ple.PF and raised under normal culture conditions have significantly improved climbing ability (from 15 to 30 days of age) compared to age-matched controls.

Flies expressing Hsap\UCP2^Scer\UAS.cFa under the control of Scer\GAL4^ple.PF and raised under normal culture conditions show no change in mitochondrial DNA/nuclear DNA ratio compared to controls.

(Islam et al., 2012)

Under fasting conditions, the hemolymph of Scer\GAL4^Ilp2.PR Hsap\UCP2^Scer\UAS.cFa flies has up to a 29% increase in circulating sugars (glucose + trehalose) compared to controls.

Scer\GAL4^Ilp2.PR-mediated expression of Hsap\UCP2^Scer\UAS.cFa extends adult lifespan and renders flies more resistant to oxidative and starvation stress compared to controls. Average egg laying activities and spontaneous physical activity are comparable to controls.

Scer\GAL4^Ilp2.PR Hsap\UCP2^Scer\UAS.cFa flies have an average of 26% increase in glycogen content but no noticeable increase in lipid content.

(Fridell et al., 2009)

Expression of Hsap\UCP2^Scer\UAS.cFa using Scer\GAL4^{elav.Switch.PO} in the presence of RU-486 extends life span on average 28% in females and 11% in males. Life span is still extended when expression is induced on day 10 of adult life, whereas induction later than day 20 has little effect.

Expression of Hsap\UCP2^Scer\UAS.cFa using Scer\GAL4^{Mhc.Switch.PO} in the presence of RU-486 has no positive effect on life span.

Mitochondria from heads of Hsap\UCP2^Scer\UAS.cFa, Scer\GAL4^{elav.Switch.PO} long-lived flies exhibit increased state 4 respiration rates and a lower respiratory control ratio.

Mitochondria from heads and thoraces of Hsap\UCP2^Scer\UAS.cFa, Scer\GAL4^{elav.Switch.PO} long-lived flies exhibit a 40% decrease in hydrogen peroxide production and a 32% decrease in 4-hydroxy-2-nonenal (a measure of oxidative damage).

Hsap\UCP2^Scer\UAS.cFa, Scer\GAL4^{elav.Switch.PO} long-lived flies have an increased resistance to paraquat, but are more sensitive to other stresses such as starvation, compared to controls.

There is no difference in egg laying, spontaneous physical activity or climbing speed between long-lived Hsap\UCP2^Scer\UAS.cFa, Scer\GAL4^{elav.Switch.PO} flies and controls.

(Fridell et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsap\UCP2^UAS.cFa, Scer\GAL4^ple.PF is a suppressor of chemical sensitive | adult stage phenotype of Drp1^UAS.Tag:HA, Scer\GAL4^ple.PF

(Hwang et al., 2014)

Hsap\UCP2^UAS.cFa, Scer\GAL4^repo is a suppressor | partially of abnormal locomotor behavior phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^repo

(Besson et al., 2010)

Hsap\UCP2^UAS.cFa, Scer\GAL4^repo is a suppressor | partially of bang sensitive phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^repo

(Besson et al., 2010)

Hsap\UCP2^UAS.cFa, Scer\GAL4^repo is a suppressor of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^repo

(Besson et al., 2010)

Hsap\UCP2^UAS.cFa, Scer\GAL4^Eaat1.PR is a suppressor of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR

(Besson et al., 2010)

Other

Statement

Reference

Hsap\UCP2^UAS.cFa, Marf^UAS.Tag:FLAG, Scer\GAL4^GMR.PU has visible phenotype

(Hwang et al., 2014)

Hsap\UCP2^UAS.cFa, Opa1^UAS.Tag:FLAG, Scer\GAL4^GMR.PU has visible phenotype

(Hwang et al., 2014)

Drp1^JF02762, Hsap\UCP2^UAS.cFa, Scer\GAL4^GMR.PU has visible phenotype

(Hwang et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

Hsap\UCP2^UAS.cFa, Scer\GAL4^ple.PF is a suppressor of dopaminergic PPL1 neuron | adult stage phenotype of Drp1^UAS.Tag:HA, Scer\GAL4^ple.PF

(Hwang et al., 2014)

Other

Statement

Reference

Hsap\UCP2^UAS.cFa, Marf^UAS.Tag:FLAG, Scer\GAL4^GMR.PU has eye phenotype

(Hwang et al., 2014)

Hsap\UCP2^UAS.cFa, Opa1^UAS.Tag:FLAG, Scer\GAL4^GMR.PU has eye phenotype

(Hwang et al., 2014)

Drp1^JF02762, Hsap\UCP2^UAS.cFa, Scer\GAL4^GMR.PU has eye phenotype

(Hwang et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\UCP2^Scer\UAS.cFa and Marf^{Scer\UAS.T:Zzzz\FLAG}, or Hsap\UCP2^Scer\UAS.cFa and Opa1^{Scer\UAS.T:Zzzz\FLAG}, or Hsap\UCP2^Scer\UAS.cFa and Drp1^JF02762 (but not Hsap\UCP2^Scer\UAS.cFa and Drp1^{Scer\UAS.T:Ivir\HA1}, Hsap\UCP2^Scer\UAS.cFa and Opa1^HMS00349, Hsap\UCP2^Scer\UAS.cFa and Marf^JF01650, Hsap\UCP2^Scer\UAS.cFa and park^Scer\UAS.cLa, or Hsap\UCP2^Scer\UAS.cFa and Pink1^{Scer\UAS.T:Ivir\HA1}), under the control of Scer\GAL4^GMR.PU results in a rough eye phenotype.

Co-expression of Hsap\UCP2^Scer\UAS.cFa suppresses the increased rotenone sensitivity seen in dopaminergic neurons of flies expressing Drp1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ple.PF.

(Hwang et al., 2014)

When Hsap\UCP2^Scer\UAS.cFa is co-expressed in the Hsap\HTT^{Q93.ex1p.Scer\UAS}, Scer\GAL4^repo background, the locomotor (climbing ability), bang sensitivity and reduced life span phenotypes are all suppressed.

When Hsap\UCP2^Scer\UAS.cFa is co-expressed in the Hsap\HTT^{Q93.ex1p.Scer\UAS}, Scer\GAL4^Eaat1.PR background, the reduced life span phenotype is suppressed.

(Besson et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

95271

w¹¹¹⁸; P{UAS-UCP2.F}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\UCP2^Scer\UAS.cFa

Hsap\UCP2^UAS.cFa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Fridell

Secondary FlyBase IDs

References (5)

Report Sections

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