FlyBase Allele Report: Dmel\Lgr3[GL01056]

Expression of Lgr3^GL01056 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of either Scer\GAL4^αTub84B.PL or Scer\GAL4^elav.PU (but not Scer\GAL4^repo or Scer\GAL4^Ptth.cMa) abrogates the growth inhibition of undamaged (anterior) tissues - eye discs - in third instar larvae (104 hr after egg laying, AEL) following targeted irradiation (limited to posterior tissues and administered in late second instar, at 80 hr AEL) which is observed in wild-type larvae and also strongly reduces the developmental delay the regenerative growth of damaged tissues normally produces. Lgr3^GL01056 expression driven by Scer\GAL4^GMR18A01 or Scer\GAL4^phm.PO combined with Scer\GAL80^elav.PY or Scer\GAL4^nSyb.PS also prevents the growth inhibition of undamaged tissue in targeted irradiation larvae, however Scer\GAL4^phm.PO-controlled expression does not decrease the irradiation damage-induced pupariation delay compared to controls.

In the absence of expression of tissue damage, expression of Lgr3^GL01056 controlled by either Scer\GAL4^αTub84B.PL or Scer\GAL4^phm.PO does not inhibit eye disc tissue growth, with the Scer\GAL4^αTub84B.PL driver as the discs are actually larger relative to age-matched controls.

(Jaszczak et al., 2016)

Expression of Lgr3^GL01056 under the control of either Scer\GAL4^nSyb.PU, Scer\GAL4^{Lgr3.T:Hsim\VP16} or Scer\GAL4^GMR19B09 results in a significant increase in fluctuating asymmetry of left and right wing areas compared to controls. Expression under the control of Scer\GAL4^P0206 has no significant effect on fluctuating asymmetry.

Expression of Lgr3^GL01056 under the control of Scer\GAL4^tub.PU results in a mild reduction in wing disc size compared to controls.

(Colombani et al., 2015)

Expression of Lgr3^GL01056 under the control of Scer\GAL4^αTub84B.PL, Scer\GAL4^arm.PS, Scer\GAL4^ChAT.7.4, Scer\GAL4^phm.PU, Scer\GAL4^GMR19B09, Scer\GAL4^GMR17G11, Scer\GAL4^Mz699 or Scer\GAL4^elav.PU does not lead to any significant delay to pupariation, as compared to controls.

Expression of Lgr3^GL01056 under the control of Scer\GAL4^elav.PU leads to an increase in fluctuating asymmetry in adult wing area, as compared to controls.

(Garelli et al., 2015)

Expression of Lgr3^GL01056 driven by Scer\GAL4^tub.PU (but not Scer\GAL4^elav.PU or Scer\GAL4^GMR9F04) accelerates pupariation.

Expression of Lgr3^GL01056 driven by Scer\GAL4^elav.PU leads to increased fluctuating asymmetry, with significant left-right variations in the size of wings.

(Vallejo et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^GMR19B09/Lgr3^GL01056 is a suppressor of abnormal developmental rate phenotype of Ilp8^{αTub84B.Tag:FLAG}

(Heredia et al., 2021, Vallejo et al., 2015)

Lgr3^GL01056, Scer\GAL4^αTub84B.PL is a suppressor of abnormal size | third instar larval stage phenotype of Ilp8^UAS.Tag:FLAG, Scer\GAL4^αTub84B.PL

(Jaszczak et al., 2016)

Lgr3^GL01056, Scer\GAL4^αTub84B.PL is a suppressor of abnormal developmental rate | larval stage phenotype of Ilp8^UAS.Tag:FLAG, Scer\GAL4^αTub84B.PL

(Jaszczak et al., 2016)

Scer\GAL4^nSyb.PU/Lgr3^GL01056 is a suppressor of abnormal developmental rate phenotype of Ecol\lexA^rn-89-lexA, Ilp8^lexAop.cCa

(Colombani et al., 2015)

Lgr3^GL01056/Scer\GAL4^VP16.Lgr3 is a suppressor of abnormal developmental rate phenotype of Ecol\lexA^rn-89-lexA, Ilp8^lexAop.cCa

(Colombani et al., 2015)

Scer\GAL4^GMR19B09/Lgr3^GL01056 is a suppressor of abnormal developmental rate phenotype of Ecol\lexA^rn-89-lexA, Ilp8^lexAop.cCa

(Colombani et al., 2015)

Lgr3^GL01056, Scer\GAL4^αTub84B.PL is a suppressor of abnormal developmental rate phenotype of Ilp8^UAS.Tag:FLAG, Scer\GAL4^αTub84B.PL

(Garelli et al., 2015)

Lgr3^GL01056, Scer\GAL4^elav.PU is a suppressor of abnormal developmental rate phenotype of Ilp8^UAS.Tag:FLAG, Scer\GAL4^elav.PU

(Garelli et al., 2015)

Lgr3^GL01056, Scer\GAL4^Tub.PU is a suppressor of abnormal developmental rate phenotype of Ilp8^UAS.Tag:FLAG, Scer\GAL4^Tub.PU

(Vallejo et al., 2015)

Scer\GAL4^elav.PU/Lgr3^GL01056 is a suppressor of abnormal developmental rate phenotype of Ilp8^{αTub84B.Tag:FLAG}

(Vallejo et al., 2015)

NOT Suppressor of

Statement

Reference

Lgr3^GL01056/Scer\GAL4^GMR18A01 is a non-suppressor of abnormal developmental rate phenotype of Ilp8^{αTub84B.Tag:FLAG}

(Heredia et al., 2021)

Lgr3^GL01056/Scer\GAL4^GMR17G11 is a non-suppressor of abnormal developmental rate phenotype of Ilp8^{αTub84B.Tag:FLAG}

(Heredia et al., 2021)

Lgr3^GL01056/Scer\GAL4^GMR18C07 is a non-suppressor of abnormal developmental rate phenotype of Ilp8^{αTub84B.Tag:FLAG}

(Heredia et al., 2021)

Lgr3^GL01056/Scer\GAL4^GMR17H01 is a non-suppressor of abnormal developmental rate phenotype of Ilp8^{αTub84B.Tag:FLAG}

(Heredia et al., 2021)

Lgr3^GL01056, Scer\GAL4^phtm.PO is a non-suppressor of abnormal size | third instar larval stage phenotype of Nos^UAS.cUa, Scer\GAL4^phtm.PO

(Jaszczak et al., 2016)

Scer\GAL4^elav.PU/Lgr3^GL01056 is a non-suppressor of abnormal size | third instar larval stage | temperature conditional phenotype of Mmus\Nos2^hs.PK

(Jaszczak et al., 2016)

Lgr3^GL01056/Scer\GAL80^elav.PU, Scer\GAL4^αTub84B.PL is a non-suppressor of abnormal developmental rate phenotype of Ilp8^UAS.Tag:FLAG, Scer\GAL4^αTub84B.PL

(Garelli et al., 2015)

Scer\GAL4^GMR9F04/Lgr3^GL01056 is a non-suppressor of abnormal developmental rate phenotype of Ilp8^{αTub84B.Tag:FLAG}

(Vallejo et al., 2015)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Lgr3^GL01056, Nos^UAS.cUa has eye disc | third instar larval stage phenotype, non-suppressible by Lgr3^GL01056/Scer\GAL4^phtm.PO

(Jaszczak et al., 2016)

Suppressor of

Statement

Reference

Lgr3^GL01056, Scer\GAL4^αTub84B.PL is a suppressor of eye disc | third instar larval stage phenotype of Ilp8^UAS.Tag:FLAG, Scer\GAL4^αTub84B.PL

(Jaszczak et al., 2016)

Lgr3^GL01056/Scer\GAL4^VP16.Lgr3 is a suppressor of wing disc phenotype of Ecol\lexA^rn-89-lexA, Ilp8^lexAop.cCa

(Colombani et al., 2015)

Scer\GAL4^GMR19B09/Lgr3^GL01056 is a suppressor of wing disc phenotype of Ecol\lexA^rn-89-lexA, Ilp8^lexAop.cCa

(Colombani et al., 2015)

Scer\GAL4^da.PU/Lgr3^GL01056 is a suppressor of phenotype of Ilp8^{αTub84B.Tag:FLAG}

(Vallejo et al., 2015)

NOT Suppressor of

Statement

Reference

Lgr3^GL01056/Scer\GAL4^phtm.PO is a non-suppressor of eye disc | third instar larval stage phenotype of Lgr3^GL01056, Nos^UAS.cUa

(Jaszczak et al., 2016)

Scer\GAL4^elav.PU/Lgr3^GL01056 is a non-suppressor of eye disc | third instar larval stage | temperature conditional phenotype of Mmus\Nos2^hs.PK

(Jaszczak et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The eye disc growth inhibition as well as the pupariation delay (in the absence of tissue damage) observed in third instar larvae expressing Ilp8^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^αTub84B.PL are suppressed by co-expression of Lgr3^GL01056 (together with UAS-Dicer2 to enhance RNAi efficiency).

Similar eye disc growth inhibition is also observed in larvae expressing Nos^Scer\UAS.cUa driven by Scer\GAL4^phm.PO and this phenotype also cannot be suppressed by co-expression of Lgr3^GL01056.

(Jaszczak et al., 2016)

Expression of Lgr3^GL01056 in neurons under the control of either Scer\GAL4^nSyb.PU, Scer\GAL4^{Lgr3.T:Hsim\VP16} or Scer\GAL4^GMR19B09 completely suppresses the developmental delay caused by expression of Ilp8^{Ecol\lexAop.cCa} in the imaginal tissues under the control of Ecol\lexA^rn-89-lexA.

Expression of Lgr3^GL01056 under the control of either Scer\GAL4^{Lgr3.T:Hsim\VP16} or Scer\GAL4^GMR19B09 completely rescues the reduction in wing disc size caused by expression of Ilp8^{Ecol\lexAop.cCa} under the control of Ecol\lexA^rn-89-lexA.

(Colombani et al., 2015)

Co-expression of Lgr3^GL01056 suppresses the developmental delay seen in flies expressing Ilp8^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PU.

Co-expression of Lgr3^GL01056 suppresses, but co-expression of both Lgr3^GL01056 and Scer\GAL80^elav.PU fails to suppress, the developmental delay seen in flies expressing Ilp8^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^αTub84B.PL.

(Garelli et al., 2015)

Co-expression of Lgr3^GL01056 suppresses the delay in pupariation seen with Ilp8^{Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^tub.PU.

Expression of Lgr3^GL01056 driven by Scer\GAL4^da.PU suppresses the increased weight phenotype seen in Ilp8^{αTub84B.T:Zzzz\FLAG} male flies.

Expression of Lgr3^GL01056 driven by Scer\GAL4^elav.PU or Scer\GAL4^GMR19B09 (but not Scer\GAL4^GMR9F04) suppresses the delay in pupariation seen in Ilp8^{αTub84B.T:Zzzz\FLAG} male flies.

(Vallejo et al., 2015)

Xenogenetic Interactions

Statement

Reference

The inhibition of eye disc growth (decreased size) in the absence of tissue damage in third instar larvae resulting from heat-shock induced ectopic expression of Mmus\Nos2^hs.PK cannot be suppressed by Scer\GAL4^elav.PU-driven expression of Lgr3^GL01056.

(Jaszczak et al., 2016)

Complementation and Rescue Data

Rescued by

Lgr3^GL01056 is rescued by Lgr3^UAS.cVa/Scer\GAL4^elav.PU

(Vallejo et al., 2015)

Comments

Co-expression of Lgr3^Scer\UAS.cVa rescues the increased wing size asymmetry seen in male flies with Lgr3^GL01056 driven by Scer\GAL4^elav.PU.

(Vallejo et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

36887

y¹ sc^* v¹ sev²¹; P{TRiP.GL01056}attP2/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Lgr3^GL01056

Name Synonyms

Secondary FlyBase IDs

References (6)

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