FlyBase Allele Report: Dmel\PGRP-LB[Δ]

General Information

Symbol

Dmel\PGRP-LB^Δ

Species

D. melanogaster

Name

FlyBase ID

FBal0267202

Feature type

allele

Associated gene

Dmel\PGRP-LB

Associated Insertion(s)

TI{TI}PGRP-LB^Δ

Carried in Construct

Also Known As

PGRP-LB^KO

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

gene targeting by homologous recombination

Nature of the Allele

Allele class

Mutagen

gene targeting by homologous recombination

(Paredes et al., 2011)

Progenitor genotype

Associated Insertion(s)

TI{TI}PGRP-LB^Δ

Cytology

Description

Part of the PGRP-LB locus has been deleted. A w^+mW.hs marker which is flanked by loxP sites is inserted in place of the deleted sequence.

(Paredes et al., 2011)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3R:11,452,842..11,460,620 [-]

Comment:

PGRP-LB sequences were deleted by homologous recombination. A w^+mW.hs marker which is flanked by loxP sites was inserted in place of the deleted sequence. The extentof the deletion was reported as 3R:7 ,278,564..7,286,342 in Release 5 coordinates.

(Paredes et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

PGRP-LB^Δ adults infected with Erwinia carotovora carotovora (Ecc) by injury exhibit a significant increase in the immune response (immune deficiency pathway activation) in fat body and midgut when they are infected by one time oral feeding, they exhibit a stronger significant increase in the immune response in fat body, proventriculus and midgut (in R1 and R4 domains (ectopically) and in R2 domain) compared to controls.

PGRP-LB^Δ adults chronically orally fed with Ecc exhibit lipid droplet depletion in fat body, organ (ovary) wasting, apoptosis in mid stage ovary follicles (pyknotic nuclei) and shortened life span compared to controls.

(Charroux et al., 2018)

PGRP-LB^Δ mutant adults have decreased lifespan and show reduced survival rate upon infection with Erwinia carotovora.

(Iatsenko et al., 2016)

Mutant flies infected with Erwinia carotovora carotovora die after 2 weeks, in contrast to wild type.

(Bosco-Drayon et al., 2012)

After septic injury with the gram-negative E. carotovora bacterium, PGRP-LB^Δ mutant flies exhibit a stronger and more sustained immune response than wild-type flies.

PGRP-LB^Δ mutant flies show a strong response to oral E. carotovora, similar to that observed after infection by septic injury with the same bacteria, in contrast to wild-type flies.

PGRP-LB^Δ flies infected with E. carotovora show reductions in mean lifespan compared to wild-type.

(Paredes et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

PGRP-LB^Δ has abnormal immune response | conditional phenotype, suppressible | partially by PGRP-LE¹¹²

(Charroux et al., 2018)

PGRP-LB^Δ has abnormal immune response | conditional phenotype, suppressible | partially by PGRP-LC^ΔE

(Charroux et al., 2018)

PGRP-LB^Δ has abnormal immune response | adult stage phenotype, suppressible by PGRP-SD^sk1

(Iatsenko et al., 2016)

PGRP-LB^Δ has short lived phenotype, suppressible by PGRP-SD^sk1

(Iatsenko et al., 2016)

PGRP-LB^Δ has abnormal immune response phenotype, suppressible by PGRP-LE¹¹²

(Bosco-Drayon et al., 2012)

NOT Suppressor of

Statement

Reference

PGRP-LB^Δ is a non-suppressor of abnormal immune response | conditional phenotype of PGRP-LE¹¹²

(Charroux et al., 2018)

Other

Statement

Reference

Df(2R)PGRP-SC^Δ, PGRP-LB^Δ, pirk^EY00723 has increased occurrence of cell division | adult stage phenotype

(Paredes et al., 2011)

PGRP-LB^Δ/Df(2R)PGRP-SC^Δ, Dredd¹, pirk^EY00723 has long lived phenotype

(Paredes et al., 2011)

PGRP-LB^Δ/Df(2R)PGRP-SC^Δ, Rel^E20, pirk^EY00723 has long lived phenotype

(Paredes et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

PGRP-LB^Δ has adult midgut | conditional phenotype, suppressible | partially by PGRP-LE¹¹²

(Charroux et al., 2018)

PGRP-LB^Δ has adult anterior midgut | conditional phenotype, suppressible | partially by PGRP-LC^ΔE

(Charroux et al., 2018)

PGRP-LB^Δ has adult fat body | conditional phenotype, suppressible | partially by PGRP-LC^ΔE

(Charroux et al., 2018)

NOT suppressed by

Statement

Reference

PGRP-LB^Δ has adult fat body | conditional phenotype, non-suppressible by PGRP-LE¹¹²

(Charroux et al., 2018)

PGRP-LB^Δ has adult posterior midgut | conditional phenotype, non-suppressible by PGRP-LC^ΔE

(Charroux et al., 2018)

PGRP-LB^Δ has adult midgut region R3 | conditional phenotype, non-suppressible by PGRP-LC^ΔE

(Charroux et al., 2018)

PGRP-LB^Δ has adult proventriculus | conditional phenotype, non-suppressible by PGRP-LE¹¹²

(Charroux et al., 2018)

NOT Suppressor of

Statement

Reference

PGRP-LB^Δ is a non-suppressor of adult proventriculus | conditional phenotype of PGRP-LE¹¹²

(Charroux et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Dredd^D55 PGRP-LB^Δ double mutant adults infected with Erwinia carotovora carotovora by oral feeding exhibit an absence of immune response (immune deficiency pathway activation) in midgut and fat body compared to controls.

(Charroux et al., 2018)

The decreased lifespan at 29[o]C as well as the precocious lethality upon Erwinia carotovora infection characteristic for PGRP-LB^Δ mutant adults is suppressed by combination with PGRP-SD^sk1.

(Iatsenko et al., 2016)

The reduced survival of PGRP-LB^Δ flies after infection with Erwinia carotovora carotovora is suppressed if they are also mutant for PGRP-LE¹¹².

(Bosco-Drayon et al., 2012)

After oral infection with E. carotovora, the addition of pirk^EY00723 to PGRP-LB^Δ; Df(3L)PGRP-SC1^Δ results in an enhancement of the immune response locally and systemically. As with septic injury, pirk^EY00723, PGRP-LB^Δ; Df(3L)PGRP-SC1^Δ flies beginning to die within 4 days of oral infection with E. carotovora.

pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ flies begin to die within 4 days of oral infection with E. carotovora. Although pirk^EY00723 Df(2R)PGRP-SC^Δ flies show the same level of response as pirk^EY00723 flies, it is important to note that the response of pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ flies is significantly higher than that of pirk^EY00723 Df(2R)PGRP-SC^Δ flies.

The stronger immune responses to E. carotovora oral infection of Df(2R)PGRP-SC^Δ PGRP-LB^Δ flies is correlated with a further decrease in lifespan compared to single mutant flies. pirk^EY00723 Df(2R)PGRP-SC^Δ; PGRP-LB^Δ mutant flies do not simply show an incremental reduction in their lifespan, but rather die rapidly with oral E. carotovora infection, decreasing 50% after only 5 days. These flies are also susceptible to oral infection with dead sonicated E. carotovora, demonstrating that is it not bacteria that are killing the fly but rather its own excessive immune response.

Dredd¹; pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ and pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ, Rel^E20 flies, with impaired imd pathway activity due to the presence of Dredd or Rel mutations, exhibit an increased lifespan upon oral infection with E. carotovora bacteria, compared to pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ flies.

pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ and Df(2R)PGRP-SC^Δ; PGRP-LB^Δ flies in unchallenged conditions exhibit a marked reduction in lifespan.

The level of epithelium renewal, as evidenced by the number of mitotic cells along the midgut, is very high in pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ flies in the absence of bacterial infection, approaching the level seen in infected wild-type guts. The mitotic index of these flies doubles between unchallenged to E. carotovora oral infection conditions, suggesting that the level of epithelium renewal in the triple mutant is approaching the limit of cells available to undergo mitosis.

pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ and pirk^EY00723, Df(2R)PGRP-SC^Δ; PGRP-LB^Δ, Df(3L)PGRP-SB^Δ5 flies are viable, although not fully fertile.

(Paredes et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

PGRP-LB^Δ is rescued by Scer\GAL4^da.G32/PGRP-LB^UAS.PA.EGFP

(Charroux et al., 2018)

PGRP-LB^Δ is rescued by Scer\GAL4^{Act5C.CoinFLP-FRT}/PGRP-LB^UAS.PA.EGFP

(Charroux et al., 2018)

PGRP-LB^Δ is rescued by PGRP-LB^UAS.PC.EGFP/Scer\GAL4^r4

(Charroux et al., 2018)

PGRP-LB^Δ is rescued by PGRP-LB^UAS.PC.EGFP/Scer\GAL4^Desat1.PB

(Charroux et al., 2018)

PGRP-LB^Δ is rescued by PGRP-LB^UAS.PC.EGFP/Scer\GAL4^da.G32

(Charroux et al., 2018)

PGRP-LB^Δ is rescued by PGRP-LB^UAS.PD.EGFP/Scer\GAL4^da.G32

(Charroux et al., 2018)

PGRP-LB^Δ is rescued by PGRP-LB^UAS.PD.EGFP/Scer\GAL4^{Act5C.CoinFLP-FRT}

(Charroux et al., 2018)

PGRP-LB^Δ/PGRP-LB^PCΔ6A is rescued by PGRP-LB^UAS.PC.EGFP/Scer\GAL4^mex1.PU

(Charroux et al., 2018)

PGRP-LB^Δ/PGRP-LB^PCΔ6A is rescued by PGRP-LB^UAS.PC.EGFP/Scer\GAL4^r4

(Charroux et al., 2018)

PGRP-LB^Δ/PGRP-LB^PCΔ10A is rescued by PGRP-LB^UAS.PC.EGFP/Scer\GAL4^mex1.PU

(Charroux et al., 2018)

PGRP-LB^Δ/PGRP-LB^PCΔ10A is rescued by PGRP-LB^UAS.PC.EGFP/Scer\GAL4^r4

(Charroux et al., 2018)

PGRP-LB^Δ is rescued by Scer\GAL4^NP0001/PGRP-LB^UAS.cGa

(Paredes et al., 2011)

PGRP-LB^Δ is rescued by PGRP-LB^UAS.cGa/Scer\GAL4^c564

(Paredes et al., 2011)

PGRP-LB^Δ is rescued by PGRP-LB^UAS.cGa/Scer\GAL4^da.G32

(Paredes et al., 2011)

Partially rescued by

PGRP-LB^Δ is partially rescued by PGRP-LB^UAS.PC.EGFP/Scer\GAL4^mex1.PU

(Charroux et al., 2018)

PGRP-LB^Δ is partially rescued by PGRP-LB^UAS.PC.EGFP/Scer\GAL4^Mef2.PR

(Charroux et al., 2018)

PGRP-LB^Δ is partially rescued by PGRP-LB^UAS.PC.EGFP/Scer\GAL4^Hml.PU

(Charroux et al., 2018)

PGRP-LB^Δ is partially rescued by PGRP-LB^UAS.PC.EGFP/Scer\GAL4^elav.PU

(Charroux et al., 2018)

Not rescued by

PGRP-LB^Δ is not rescued by PGRP-LB^{UAS.PA-C160S.EGFP}/Scer\GAL4^mex1.PU

(Charroux et al., 2018)

PGRP-LB^Δ is not rescued by Scer\GAL4^Desat1.PB/PGRP-LB^UAS.PA.EGFP

(Charroux et al., 2018)

PGRP-LB^Δ is not rescued by Scer\GAL4^Mef2.PR/PGRP-LB^UAS.PA.EGFP

(Charroux et al., 2018)

PGRP-LB^Δ is not rescued by PGRP-LB^UAS.PA.EGFP/Scer\GAL4^r4

(Charroux et al., 2018)

PGRP-LB^Δ is not rescued by Scer\GAL4^mex1.PU/PGRP-LB^UAS.PA.EGFP

(Charroux et al., 2018)

PGRP-LB^Δ is not rescued by Scer\GAL4^elav.PU/PGRP-LB^UAS.PA.EGFP

(Charroux et al., 2018)

PGRP-LB^Δ is not rescued by PGRP-LB^UAS.PA.EGFP/Scer\GAL4^Hml.PU

(Charroux et al., 2018)

PGRP-LB^Δ is not rescued by Scer\GAL4^mex1.PU/PGRP-LB^{UAS.PC-C177S.EGFP}

(Charroux et al., 2018)

PGRP-LB^Δ is not rescued by Scer\GAL4^Desat1.PB/PGRP-LB^{UAS.PC-C177S.EGFP}

(Charroux et al., 2018)

PGRP-LB^Δ is not rescued by Scer\GAL4^Mef2.PR/PGRP-LB^{UAS.PC-C177S.EGFP}

(Charroux et al., 2018)

PGRP-LB^Δ is not rescued by Scer\GAL4^mex1.PU/PGRP-LB^{UAS.PD-C200S.EGFP}

(Charroux et al., 2018)

PGRP-LB^Δ is not rescued by Scer\GAL4^Desat1.PB/PGRP-LB^UAS.PD.EGFP

(Charroux et al., 2018)

PGRP-LB^Δ is not rescued by PGRP-LB^UAS.PD.EGFP/Scer\GAL4^r4

(Charroux et al., 2018)

PGRP-LB^Δ is not rescued by PGRP-LB^UAS.PD.EGFP/Scer\GAL4^Mef2.PR

(Charroux et al., 2018)

PGRP-LB^Δ is not rescued by Scer\GAL4^mex1.PU/PGRP-LB^UAS.PD.EGFP

(Charroux et al., 2018)

PGRP-LB^Δ is not rescued by Scer\GAL4^elav.PU/PGRP-LB^UAS.PD.EGFP

(Charroux et al., 2018)

PGRP-LB^Δ is not rescued by PGRP-LB^UAS.PD.EGFP/Scer\GAL4^Hml.PU

(Charroux et al., 2018)

Comments

Expression of PGRP-LB^UAS.PA.EGFP under the control of Scer\GAL4^mex1.PU partially rescues the immune system response in midgut but not in fat body phenotype of PGRP-LB^Δ adults infected with Erwinia carotovora carotovora (Ecc) by oral feeding. It fails to rescue the lipid droplets in fat body and the life span phenotype of PGRP-LB^Δ adults.

Expression of PGRP-LB^UAS.PA.EGFP under the control of Scer\GAL4^da.G32 strongly rescues the lipid droplets in fat body, the ovary wasting, the apoptosis in ovary follicles and the life span phenotype while it fails to rescue the immune system response in fat body and proventriculus phenotype of PGRP-LB^Δ adults infected with Ecc by oral feeding.

Expression of PGRP-LB^UAS.PC.EGFP under the control of Scer\GAL4^mex1.PU rescues the lipid droplets in fat body phenotype while it partially rescues the immune system response in midgut and fat body and the life span phenotype of PGRP-LB^Δ adults infected with Ecc by oral feeding.

Expression of PGRP-LB^UAS.PC.EGFP under the control of Scer\GAL4^Desat1.PB or Scer\GAL4^Mef2.PR rescues the immune system response in fat body phenotype; its expression under the control of Scer\GAL4^Mef2.PR but not Scer\GAL4^Desat1.PB partially rescues the same phenotype in midgut; and its expression under the control of Scer\GAL4^Mef2.PR partially rescues the life span phenotype of PGRP-LB^Δ adults infected with Ecc by oral feeding.

Expression of PGRP-LB^UAS.PD.EGFP under the control of Scer\GAL4^mex1.PU rescues the immune system response in midgut but not in fat body; its expression under the control of Scer\GAL4^Mef2.PR partially rescues the same phenotype in fat body but not in midgut; and its expression under the control of Scer\GAL4^mex1.PU or Scer\GAL4^Mef2.PR fails to rescue the life span and the lipid droplets in fat body (only with Scer\GAL4^mex1.PU) phenotype of PGRP-LB^Δ adults infected with Ecc by oral feeding.

Expression of PGRP-LB^UAS.PD.EGFP under the control of Scer\GAL4^da.G32 strongly rescues the lipid droplets in fat body, the ovary wasting, the apoptosis in ovary follicles and the life span phenotype while it fails to rescue the immune system response in fat body and proventriculus phenotype of PGRP-LB^Δ adults infected with Ecc by oral feeding.

(Charroux et al., 2018)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

55715

w^*; TI{TI}PGRP-LB^Δ

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

PGRP-LB KO

(Liu et al., 2022)

PGRP-LB^KO

(Tavignot et al., 2017)

PGRP-LB^ko

(Masuzzo et al., 2022)

PGRP-LB^Δ

(Charroux and Royet, 2022, Charroux et al., 2018, Iatsenko et al., 2018, Kurz et al., 2017, Guillou et al., 2016, Iatsenko et al., 2016, Gendrin et al., 2013, Bosco-Drayon et al., 2012, Neyen et al., 2012, Paredes et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (13)

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